公开(公告)号：DE69121635T2

公开(公告)日：1997-04-03

申请号：DE69121635

申请日：1991-06-03

Applicant: ABBOTT LAB

Inventor： GARNER WILLIAM D ,  CODACOVI LYNN MARIE ,  MATTINGLY PHILLIP GREGORY ,  WELCH CHRISTOPHER JOSEPH ,  KUEMMERLE STEVEN C ,  DELBY SUZANNE M ,  LANE THERESA L

IPC: C12Q1/42 ,  C07K17/08 ,  G01N33/543 ,  G01N33/82 ,  G01N33/546 ,  G01N33/545

Abstract: The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase linked to affinity purified intrinsic factor with a first linking group at least three angstroms long. The first conjugate and the bound cobalamins in the sample are then exposed to a second conjugate of a cobalamin linked to a detectable enzyme to produce second conjugate bound to first conjugate and unbound second conjugate. The enzyme activity associated either with the solid phase first conjugate or the unbound second conjugate is then detected. By linking affinity purified intrinsic factor to a latex or latex-like solid phase with a first linking group at least three angstroms long, an enzyme assay can be performed which detects levels of cobalamins in patient samples within or below normal ranges found in such samples.

公开(公告)号：CA2135887A1

公开(公告)日：1993-12-23

申请号：CA2135887

申请日：1993-06-09

Applicant: ABBOTT LAB

Inventor： BAKER HAROLD N ,  ENG KATHERINE K ,  GARNER WILLIAM D ,  MASSEI MICHAEL K ,  NECKLAWS ELIZABETH C ,  OSIKOWICZ EUGENE W ,  RAMP SALLY K ,  TRACH PAULA

IPC: G01N33/53 ,  C07J1/00 ,  C07J41/00 ,  C12N9/16 ,  C12N9/96 ,  G01N33/535 ,  G01N33/543 ,  G01N33/58 ,  G01N33/74 ,  C12N9/14 ,  G01N33/547

Abstract: 2135887 9325672 PCTABS00028 The present invention relates to the measurement of estradiol using competitive immunoassay methods. The inventors unexpectedly discovered that estrone and its derivatives conjugated to a label is a particularly effective tracer when used in conjunction with estradiol specific antibodies to determine estradiol levels in fluid samples. The present invention also utilizes 5.alpha.-dihydrotestosterone to enhance the assay performance.

公开(公告)号：ES2093655T3

公开(公告)日：1997-01-01

申请号：ES91109036

申请日：1991-06-03

Applicant: ABBOTT LAB

Inventor： GARNER WILLIAM D ,  CODACOVI LYNN MARIE ,  MATTINGLY PHILLIP GREGORY ,  WELCH CHRISTOPHER JOSEPH ,  KUEMMERLE STEVEN C ,  DELBY SUZANNE M

IPC: C12Q1/42 ,  C07K17/08 ,  G01N33/543 ,  G01N33/82 ,  G01N33/546 ,  G01N33/545

Abstract: The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase linked to affinity purified intrinsic factor with a first linking group at least three angstroms long. The first conjugate and the bound cobalamins in the sample are then exposed to a second conjugate of a cobalamin linked to a detectable enzyme to produce second conjugate bound to first conjugate and unbound second conjugate. The enzyme activity associated either with the solid phase first conjugate or the unbound second conjugate is then detected. By linking affinity purified intrinsic factor to a latex or latex-like solid phase with a first linking group at least three angstroms long, an enzyme assay can be performed which detects levels of cobalamins in patient samples within or below normal ranges found in such samples.

公开(公告)号：DE69121635D1

公开(公告)日：1996-10-02

申请号：DE69121635

申请日：1991-06-03

Applicant: ABBOTT LAB

Inventor： GARNER WILLIAM D ,  CODACOVI LYNN MARIE ,  MATTINGLY PHILLIP GREGORY ,  WELCH CHRISTOPHER JOSEPH ,  KUEMMERLE STEVEN C ,  DELBY SUZANNE M ,  LANE THERESA L

IPC: C12Q1/42 ,  C07K17/08 ,  G01N33/543 ,  G01N33/82 ,  G01N33/546 ,  G01N33/545

Abstract: The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase linked to affinity purified intrinsic factor with a first linking group at least three angstroms long. The first conjugate and the bound cobalamins in the sample are then exposed to a second conjugate of a cobalamin linked to a detectable enzyme to produce second conjugate bound to first conjugate and unbound second conjugate. The enzyme activity associated either with the solid phase first conjugate or the unbound second conjugate is then detected. By linking affinity purified intrinsic factor to a latex or latex-like solid phase with a first linking group at least three angstroms long, an enzyme assay can be performed which detects levels of cobalamins in patient samples within or below normal ranges found in such samples.

公开(公告)号：CA2043862A1

公开(公告)日：1991-12-07

申请号：CA2043862

申请日：1991-06-04

Applicant: ABBOTT LAB

Inventor： GARNER WILLIAM D ,  CODACOVI LYNN M ,  MATTINGLY PHILLIP G ,  WELCH CHRISTOPHER J ,  KUEMMERLE STEVEN C ,  DELBY SUZANNE M ,  LANE THERESA L

IPC: C12Q1/42 ,  C07K17/08 ,  G01N33/543 ,  G01N33/82 ,  G01N33/544 ,  G01N33/535

Abstract: The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase linked to affinity purified intrinsic factor with a first linking group at least three angstroms long. The first conjugate and the bound cobalamins in the sample are then exposed to a second conjugate of a cobalamin linked to a detectable enzyme to produce second conjugate bound to first conjugate and unbound second conjugate. The enzyme activity associated either with the solid phase first conjugate or the unbound second conjugate is then detected. By linking affinity purified intrinsic factor to a latex or latex-like solid phase with a first linking group at least three angstroms long, an enzyme assay can be performed which detects levels of cobalamins in patient samples within or below normal ranges found in such samples.