公开(公告)号：US20240210321A1

公开(公告)日：2024-06-27

申请号：US18567981

申请日：2022-06-07

Applicant: CytoVeris Inc.

Inventor： Rishikesh Pandey ,  Alan Kersey ,  Gary Root ,  Aditya Shirvalkar

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/645 ,  G01N2021/6417 ,  G01N2201/1296

Abstract: A method and system of analyzing an ex-vivo tissue sample is provided. The method includes interrogating the tissue sample a plurality of times, each interrogation using at least one excitation light centered on a wavelength distinct from the others, at least one excitation light produces AF emissions from one or more biomolecules associated with the tissue sample, and another is produces diffuse reflectance signals from the tissue sample; b) using a photodetector to detect the AF emissions or diffuse reflectance signals from the tissue sample, producing photodetector signals representative thereof; c) processing the photodetector signals attributable to the AF emissions using a first trained classifier to determine first data sets indicative of biomolecules; d) processing the photodetector signals attributable to the diffuse reflectance signals using a second trained classifier to determine one or more second data sets; and e) determining a type of the tissue sample.

公开(公告)号：US20220283093A1

公开(公告)日：2022-09-08

申请号：US17688792

申请日：2022-03-07

Applicant: CytoVeris Inc.

Inventor： Rishikesh Pandey ,  Alan Kersey

IPC: G01N21/65 ,  G01N21/64

Abstract: A method and system for classifying a tissue specimen is provided. The method includes: a) interrogating a tissue specimen with a first interrogation light; b) detecting first light from the tissue specimen resulting from the interrogation, wherein the first light includes a first scattered light component and a first fluorescence light component, and producing first signals; c) interrogating the tissue specimen with a second interrogation light at a second excitation wavelength, wherein the first and second excitation wavelengths are within about 2 nm of each other; d) detecting second light from the tissue specimen resulting from the interrogation, wherein the second light includes a second scattered light component and a second fluorescence light component, and producing second signals; e) determining a difference between the first and second lights; f) determining a fluorescence spectrum produced by the interrogation of the tissue specimen; and g) classifying the tissue specimen.

公开(公告)号：US20220196557A1

公开(公告)日：2022-06-23

申请号：US17601631

申请日：2020-04-06

Applicant: CYTOVERIS INC.

Inventor： Guoan Zheng ,  Alan Kersey ,  Rishikesh Pandey ,  David Fournier

IPC: G01N21/65 ,  G01N21/64 ,  G01J3/44

Abstract: An apparatus and method for analyzing a tissue sample to provide depth-selective information includes at least one light source, collection light optics, and a light detector. The light source is configured to produce a light beam having one or more wavelengths of light that cause a tissue sample to produce Raman light signals upon interrogation of the tissue sample. The light beam is oriented to impinge on an exposed surface of the tissue sample at a point of incidence (POI), and oriented so that the light beam enters the tissue sample at an oblique angle relative to the exposed surface of the tissue sample. The collection light optics are configured to collect the Raman light signals emanating from the tissue sample at one or more predetermined lateral distances from the point of incidence. The light detector is configured to receive the Raman light signals from the collection light optics.

公开(公告)号：US20230346983A1

公开(公告)日：2023-11-02

申请号：US17796536

申请日：2021-02-01

Applicant: CytoVeris Inc.

Inventor： Alan Kersey ,  Rishikesh Pandey ,  Patrick Curry

IPC: A61K49/00 ,  A61B5/00 ,  A61K41/00

CPC classification number: A61K49/001 ,  A61B5/0075 ,  A61B5/0091 ,  A61K41/0052

Abstract: A method and system for determining the presence of a mass of cancerous cells in vivo within a tissue body is provided. The method includes: a) performing an examination of the tissue body using a diagnostic method operable to determine the presence of a suspect tissue mass, and determining a location of the same; b) administering a solution containing “RR-CTEs”, the RR-CTEs configured to target and bind with cancerous cells; c) interrogating the tissue body with a beam of light, wherein the RR-CTEs are configured to produce Raman scattered light upon impingement; d) collecting the Raman scattered light; e) processing the collected Raman scattered light to determine a presence or an absence of the a Raman signature; and f) comparing the determined location of the suspect tissue mass with the determined location of the mass of cancerous cells to determine the presence of the mass of cancerous cells.

公开(公告)号：US20230266170A1

公开(公告)日：2023-08-24

申请号：US18017812

申请日：2021-07-26

Applicant: CytoVeris Inc.

Inventor： Alan Kersey ,  David Fournier ,  Rishikesh Pandey ,  Gary Root

IPC: G01J3/44 ,  G01J3/12 ,  G01J3/02 ,  G01J3/08 ,  G01J3/10

CPC classification number: G01J3/44 ,  G01J3/12 ,  G01J3/0208 ,  G01J3/08 ,  G01J3/10 ,  G01J3/0218 ,  G01J2003/102 ,  G01J2003/1213 ,  G01N21/65

Abstract: A system and method for analyzing a sample using Raman spectral light includes a light source, a light detector, a narrow band pass filter and an analyzer. Within the system, excitation light is directed to interrogate the sample. The narrow band pass filter is positioned to receive Raman scattered light produced as a result of the interrogation. The light detector is positioned to receive the Raman scattered light that has passed through the at least one narrow band pass filter. The analyzer contains stored instructions that when executed cause the processor to a) control the light source; and b) process signals produced by the light detector to analyze the sample material, the signals representative of the intensity of the Raman scattered light received by the at least one light detector corresponding to one or more wavenumbers in a high wavenumber region of a Raman signal.

公开(公告)号：US20230162410A1

公开(公告)日：2023-05-25

申请号：US17992613

申请日：2022-11-22

Applicant: CytoVeris Inc.

Inventor： Rishikesh Pandey ,  Guoan Zheng ,  Alan Kersey

IPC: G06T11/00 ,  G06T7/30 ,  G06T3/40 ,  G01N21/64

CPC classification number: G06T11/001 ,  G01N21/6486 ,  G06T3/40 ,  G06T7/30 ,  G06T2207/10064 ,  G06T2207/10152 ,  G06T2207/20016 ,  G06T2207/30024 ,  G06T2210/41

Abstract: A system for and method of producing a virtually stained histological tissue sample is provided that includes: a) acquiring a plurality of autofluorescence (AF) images of an unstained tissue sample, each AF image of the plurality of images produced by interrogating the tissue sample at an AF excitation wavelength configured to produce AF emissions at an AF emission wavelength, wherein the AF excitation wavelength and the AF emission wavelength used to produce each AF image of the plurality of AF images is different from the AF excitation wavelength and the AF emission wavelength used to produce the other AF images of the plurality of AF images; b) virtually staining the tissue sample using the plurality of AF images using artificial intelligence to represent a coloration of at least one histological stain; and c) producing a virtually stained histological tissue sample from the virtual staining.

公开(公告)号：US20220079450A1

公开(公告)日：2022-03-17

申请号：US17478513

申请日：2021-09-17

Applicant: CytoVeris Inc.

Inventor： David Fournier ,  Rishikesh Pandey ,  Michael A. Sapack ,  Patrick M. Curry ,  Alan Kersey

IPC: A61B5/00

Abstract: A system and method for determining the presence of cancerous tissue within a tissue cavity is provided. The system includes one or more excitation light sources, a photodetector, a probe, and a system controller. The probe includes an optically transparent probe body configured to fit within the tissue cavity. The system controller is in communication with the excitation light sources, the photodetector, and a memory storing instructions. The instructions when executed cause the system controller to a) control the excitation light sources to produce excitation light beams within the probe body, the excitation light beams operable to produce a response of the tissue to the interrogation and control the photodetector to detect the response and produce signals representative thereof; and b) produce information indicative of a presence of the cancerous tissue using the signals representative of the response.

公开(公告)号：US20220057263A1

公开(公告)日：2022-02-24

申请号：US17408976

申请日：2021-08-23

Applicant: CytoVeris Inc.

Inventor： Alan Kersey ,  Rishikesh Pandey ,  David Fournier ,  Gary Root

IPC: G01J3/02 ,  G01J3/44 ,  G02B26/08 ,  G01N21/65

Abstract: A system for processing Raman scattering light from a sample is provided. The system includes a source, a digital mirror device (DMD), a detector, and an analyzer. The DMD is configured to reflect Raman scattering light and includes micromirrors selectively controllable between ON and OFF states. The detector is configured to detect Raman scattering light and to produce signals representative of the Raman scattering light. The analyzer is in communication with the light source, the DMD, the detector, and a memory storing instructions, which instructions when executed cause the processor to: a) control the light source to produce a beam of light for interrogating the sample; b) control the DMD to place in an ON or OFF state based on one or more known spectral shapes stored in the memory; and c) process the Raman scattering light reflected by the micromirrors in the ON state.

公开(公告)号：US20250082205A1

公开(公告)日：2025-03-13

申请号：US18882678

申请日：2024-09-11

Applicant: CytoVeris Inc.

Inventor： Alan Kersey ,  Rishikesh Pandey

IPC: A61B5/00

Abstract: A method and system for analyzing a tissue, the method includes: administering a fluorescent agent to the tissue; producing a first excitation light configured to produce an autofluorescence emission from a biomolecule present within the tissue, and a diffuse reflectance signal from the tissue; producing a second excitation light configured to produce a fluorescence emission from the fluorescent agent administered to the tissue; using a photodetector to detect the autofluorescence emissions, the diffuse reflectance signals and produce first signals representative of the autofluorescence emission and the diffuse reflectance; using the photodetector to detect the fluorescence emissions and produce second signals representative of the fluorescence emission; producing a first image using the first signals; producing a second image using the second signals; and analyzing the tissue using the first image and the second image to identify the presence of diseased tissue within the tissue.

公开(公告)号：US12163891B2

公开(公告)日：2024-12-10

申请号：US17627287

申请日：2020-07-16

Applicant: CytoVeris Inc.

Inventor： Alan Kersey ,  Rishikesh Pandey

IPC: G01N21/65 ,  G02B26/08 ,  G02B26/10 ,  A61N5/06

Abstract: A system and method for mapping a tissue sample is provided. The system includes a light source, a scanner, a digital mirror device (DMD), a light detector, and an analyzer. The DMD has an array of micromirrors. The analyzer controls the light source, controls the scanner, controls the DMD to have on-state micromirrors aligned with a light beam, and other micromirrors in an off-state. The on-state micromirrors direct the light beam to a tissue sample. The analyzer assigns one or more location codes to the on-state micromirrors, controls the light detector to receive Raman light emitted from the tissue sample, correlates the location codes of the on-state micromirrors with light detector signals representative of the Raman emitted light, and produces a spatial map of the Raman emitted light.