公开(公告)号：WO2020198614A1

公开(公告)日：2020-10-01

申请号：PCT/US2020/025296

申请日：2020-03-27

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： LI, Stephen K.H. ,  MAJONIS, Daniel ,  BARANOV, Vladimir ,  ORNATSKY, Olga ,  ALLO, Bedilu ,  BANDURA, Dmitry ,  THOM, Colin ,  STELZER, Greg ,  LOBODA, Alexander

IPC: A61K9/19 ,  G01N15/10 ,  G01N27/62 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532

Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.

公开(公告)号：WO2021127435A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/066012

申请日：2020-12-18

Applicant: FLUIDIGM CANADA INC.

Inventor： ALLO, Bedilu ,  LOU, Xudong ,  LIU, Peng ,  MAJONIS, Daniel ,  MAVROPOULOS, Anastasia ,  GHEIRATMAND, Ladan

IPC: C08F20/10 ,  C08F20/56 ,  C08F220/36 ,  C08F220/54 ,  C08F220/56

Abstract: Described herein are reagents and methods for improving signal in imaging mass cytometry. Aspects include mass tags with a large number of labeling atoms, chemical modifications to mass tags and additional reagents to reduce background and/or maintain target binding of mass tagged specific binding partners (SBPs), and schemes for associating a plurality of mass tags with a single SBP. As such, embodiments include any combination of one or more reagents and their use. The reagents, kits and methods herein may be used for mass cytometry, including imaging mass cytometry. In some aspects, reagents, kits or methods may be used for delivery of a large number of radioisotopes to a target analyte, for example for therapeutic use or radiometric detection. In certain aspects, only non-radioactive isotopes may be used for mass cytometry.

公开(公告)号：WO2019089088A1

公开(公告)日：2019-05-09

申请号：PCT/US2018/036622

申请日：2018-06-08

Applicant: FLUIDIGM CANADA INC. ,  CHANG, Qing ,  ALLO, Bedilu ,  LUO, Xudong ,  BOUZEKRI, Alexander

Inventor： CHANG, Qing ,  ALLO, Bedilu ,  LUO, Xudong ,  BOUZEKRI, Alexander ,  ORNATSKY, Olga

IPC: C07K1/13 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/68

Abstract: The present disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.

公开(公告)号：EP3704133A1

公开(公告)日：2020-09-09

申请号：EP18874977.4

申请日：2018-06-08

Applicant: Fluidigm Canada Inc. ,  Chang, Qing ,  Allo, Bedilu ,  Luo, Xudong ,  Bouzekri, Alexander

Inventor： CHANG, Qing ,  ALLO, Bedilu ,  LUO, Xudong ,  BOUZEKRI, Alexander ,  ORNATSKY, Olga

IPC: C07K1/13 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/68

公开(公告)号：EP3946274A1

公开(公告)日：2022-02-09

申请号：EP20779263.1

申请日：2020-03-27

Applicant: Fluidigm Canada Inc.

Inventor： LI, Stephen K.H. ,  MAJONIS, Daniel ,  BARANOV, Vladimir ,  ORNATSKY, Olga ,  ALLO, Bedilu ,  BANDURA, Dmitry ,  THOM, Colin ,  STELZER, Greg ,  LOBODA, Alexander

IPC: A61K9/19 ,  G01N15/10 ,  G01N27/62 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532

公开(公告)号：EP3784768A1

公开(公告)日：2021-03-03

申请号：EP19793849.1

申请日：2019-04-26

Applicant: Fluidigm Canada Inc.

Inventor： LOBODA, Alexander V. ,  ORNATSKY, Olga ,  BARANOV, Vladimir ,  ZABINYAKOV, Nikita ,  MAVROPOULOS, Anastasia ,  MAJONIS, Daniel ,  ALLO, Bedilu ,  CLOSSON, Taunia

IPC: C12M1/00 ,  C12M1/34 ,  G01N35/00 ,  G01N35/10 ,  H01J49/00 ,  H01J49/02