公开(公告)号：WO2020198614A1

公开(公告)日：2020-10-01

申请号：PCT/US2020/025296

申请日：2020-03-27

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： LI, Stephen K.H. ,  MAJONIS, Daniel ,  BARANOV, Vladimir ,  ORNATSKY, Olga ,  ALLO, Bedilu ,  BANDURA, Dmitry ,  THOM, Colin ,  STELZER, Greg ,  LOBODA, Alexander

IPC: A61K9/19 ,  G01N15/10 ,  G01N27/62 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532

Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.

公开(公告)号：WO2014190429A1

公开(公告)日：2014-12-04

申请号：PCT/CA2014/050474

申请日：2014-05-22

Applicant: FLUIDIGM CANADA INC.

Inventor： BANDURA, Dmitry ,  BARANOV, Vladimir ,  LOBODA, Alexander ,  ORNATSKY, Olga ,  TANNER, Scott

IPC: G01N27/62 ,  G01N33/483

CPC classification number: G01N33/54366 ,  G01N15/1031 ,  G01N15/1404 ,  G01N15/1459 ,  G01N27/622 ,  G01N27/624 ,  G01N33/483 ,  G01N33/532 ,  G01N33/54313 ,  G01N2015/1006 ,  G01N2458/15

Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.

Abstract translation: 本发明涉及用于分子细胞计数的方法和装置。 分子光谱仪用于分子标签标记颗粒的分析。 在分子细胞仪中，附着于粒子（如细胞）的挥发性分子标记作为分子标签的基团释放，并通过软电离方法离子化以产生相应的分子离子组。 分子细胞仪有两个检测器，一个用于检测分子离子组的存在，另一个用于检测迁移率分离的分子离子。 迁移率分离的分子离子被同步以对应于分子离子的组。

公开(公告)号：WO2021127657A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/066488

申请日：2020-12-21

Applicant: FLUIDIGM CANADA INC.

Inventor： CAREW, Adam ,  SANDKUIJL, Daaf ,  LOBODA, Alexander ,  WEEL, Matthew ,  BANDURA, Dmitry

IPC: G01N15/10 ,  H01J49/26 ,  G01N15/00 ,  G01N27/62 ,  H01J49/04 ,  H01J49/10

Abstract: Described herein are systems and methods for imaging mass spectrometry, including imaging mass cytometry. Aspects of the subject application include apparatus and methods for imaging mass spectrometry (IMS) that improve speed of sample acquisition, signal sensitivity, and/or signal stability. Systems and methods described herein may minimize the transfer time and/or may minimize the spread of plumes of sample material ablated from a sample to be transferred to the components of the imaging mass spectrometer or mass cytometer that ionize and analyze the sample material.

公开(公告)号：EP3946274A1

公开(公告)日：2022-02-09

申请号：EP20779263.1

申请日：2020-03-27

Applicant: Fluidigm Canada Inc.

Inventor： LI, Stephen K.H. ,  MAJONIS, Daniel ,  BARANOV, Vladimir ,  ORNATSKY, Olga ,  ALLO, Bedilu ,  BANDURA, Dmitry ,  THOM, Colin ,  STELZER, Greg ,  LOBODA, Alexander

IPC: A61K9/19 ,  G01N15/10 ,  G01N27/62 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532

公开(公告)号：EP3004860A1

公开(公告)日：2016-04-13

申请号：EP14804873.9

申请日：2014-05-22

Applicant: Fluidigm Canada Inc.

Inventor： BANDURA, Dmitry ,  BARANOV, Vladimir ,  LOBODA, Alexander ,  ORNATSKY, Olga ,  TANNER, Scott

IPC: G01N27/62 ,  G01N33/483

CPC classification number: G01N33/54366 ,  G01N15/1031 ,  G01N15/1404 ,  G01N15/1459 ,  G01N27/622 ,  G01N27/624 ,  G01N33/483 ,  G01N33/532 ,  G01N33/54313 ,  G01N2015/1006 ,  G01N2458/15

Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.

Abstract translation: 本发明涉及用于分子细胞计数的方法和装置。 分子细胞仪用于分子标签标记颗粒的分析。 在分子细胞仪中，附着于颗粒（如细胞）的挥发性分子标签作为分子标签的基团释放，并通过软电离方法离子化，产生相应的分子离子组。 分子细胞仪有两个检测器，一个用于检测分子离子组的存在，另一个用于检测迁移率分离的分子离子。 迁移率分离的分子离子被同步以与分子离子的组对应。