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公开(公告)号：WO2013138767A1
公开(公告)日：2013-09-19
申请号：PCT/US2013/032420
申请日：2013-03-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： PALLAS, Michael, C. , NURSE, James , LIM, Gary , STRAUB, Theodore , GHENCIU, Eliodor , FOSTER, Evan , FONSECA, Jorge , MAHER, Kevin
CPC classification number: B01L3/50857 , B01L3/50 , B01L3/50851 , B01L2200/141 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , C12Q1/6806 , C12Q1/686 , G03F7/2002
Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.
Abstract translation: 提供了用于进行生物反应的系统。 该系统包括包括基底和多个反应位点的芯片。 多个反应位点各自被配置为包括至多一个纳升级的液体样品。 此外，该系统包括被配置为启动液体样品内的生物反应的控制系统。 该系统还包括被配置为检测芯片上的生物反应的检测系统。 根据各种实施方案，该芯片包括至少20000个反应位点。 在其它实施方案中，芯片包括至少30000个反应位点。
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公开(公告)号：WO2011127386A3
公开(公告)日：2011-10-13
申请号：PCT/US2011/031750
申请日：2011-04-08
Applicant: LIFE TECHNOLOGIES CORPORATION , CONNER, Thomas, A , LIM, Chee Kiong , PALLAS, Michael, C. , CHING, Chee Wee
Inventor： CONNER, Thomas, A , LIM, Chee Kiong , PALLAS, Michael, C. , CHING, Chee Wee
Abstract: A method for performing polymerase chain reactions (PCR) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. The method further includes calculating, by a thermoelectric controller, a difference in temperature between the first temperature and the second temperature and adjusting, by the thermoelectric controller, the first temperature of the first sample block sector based on the difference in temperature by using one or more thermoelectric coolers. The one or more thermoelectric coolers is configured to heat or cool the first sample block sector by adjusting power output from the thermoelectric controller.
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公开(公告)号：EP2825314A1
公开(公告)日：2015-01-21
申请号：EP13713049.8
申请日：2013-03-15
Applicant: Life Technologies Corporation
Inventor： PALLAS, Michael, C. , NURSE, James , LIM,Gary , STRAUB, Theodore , GHENCIU, Eliodor , FOSTER, Evan , FONSECA, Jorge , MAHER, Kevin
CPC classification number: B01L3/50857 , B01L3/50 , B01L3/50851 , B01L2200/141 , B01L2300/0893 , B01L2300/0896 , B01L2300/161 , C12Q1/6806 , C12Q1/686 , G03F7/2002
Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.
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