公开(公告)号：WO2011094577A3

公开(公告)日：2011-11-03

申请号：PCT/US2011022973

申请日：2011-01-28

Applicant: MICRONICS INC ,  BATTRELL C FREDERICK ,  SPRAGUE ISAAC ,  BRAGD MATTHEW SCOTT ,  CAPODANNO JASON

Inventor： BATTRELL C FREDERICK ,  SPRAGUE ISAAC ,  BRAGD MATTHEW SCOTT ,  CAPODANNO JASON

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/52 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0684 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0638 ,  G01N21/03 ,  G01N21/0332 ,  G01N21/17 ,  G01N21/64 ,  G01N21/6428 ,  Y10T137/0318

Abstract: A microfluidic cartridge and methods for performing a diagnostic, molecular or biochemical assay thereon, where all dried and/or liquid reagents necessary for the assay are contained in the cartridge and the assay requires only the addition of sample. Pneumohydraulic features, chamber and diaphragm technologies are introduced for overcoming the problems of bubble interference and reagent washout during operation of a microfluidic cartridge. The cartridges are inserted into a host instrument for performance of an assay and the cartridge is supplied as a consumable.

Abstract translation: 微流控筒和用于在其上进行诊断，分子或生物化学测定的方法，其中所述测定所需的所有干燥和/或液体试剂都包含在所述盒中，并且所述测定仅需添加样品。 引入气动液压特性，腔室和隔膜技术，以克服在微流控筒操作期间气泡干扰和试剂冲洗的问题。 将盒式磁盘插入到主仪器中以进行测定，并且将盒作为消耗品提供。

公开(公告)号：WO2014100743A2

公开(公告)日：2014-06-26

申请号：PCT/US2013077269

申请日：2013-12-20

Applicant: MICRONICS INC

Inventor： SPRAGUE ISAAC ,  KAY JUSTIN L ,  BRAGD MATTHEW SCOTT ,  BATTRELL C FREDERICK

IPC: F04B9/02

CPC classification number: B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2400/06 ,  F04B19/006 ,  F04B43/043 ,  F16K99/0015 ,  F16K99/0055 ,  F16K2099/0084

Abstract: Microfluidic circuit elements, such as a microvalve, micropump or microvent, formed of a microcavity divided by a diaphragm web into a first subcavity bounded by a first internal wall and a second subcavity bounded by a second internal wall, where the diaphragm web is characterized as a thin film having a first state contacting the first internal wall and a second state contacting the second internal wall and exhibiting essentially no elasticity in moving between the first state and the second state, the thin film web having been stretched beyond its yield point before or during use are provided. The disclosed elements enable faster and more efficient cycling of the diaphragm in the microcavity and increases the diaphragm surface area. In a preferred embodiment, the microfluidic circuit element is pneumatically driven and controls the motion of fluids in a microassay device.

Abstract translation: 微流体回路元件，例如微型阀，微型泵或微型阀，其由被隔膜腹板分成的微腔形成为由第一内壁和由第二内壁界定的第二子腔界定的第一子腔，其中隔膜腹板的特征在于 具有与第一内壁接触的第一状态和与第二内壁接触并且在第一状态和第二状态之间移动时基本上没有弹性的第二状态的薄膜，所述薄膜卷材在之前已经被拉伸超过其屈服点或 在使用期间提供。 所公开的元件能够更快速和更有效地使微腔中的隔膜循环并且增加隔膜表面积。 在一个优选实施例中，微流体回路元件是气动驱动的并且控制微测装置中的流体的运动。

公开(公告)号：CA2786569A1

公开(公告)日：2011-08-04

申请号：CA2786569

申请日：2011-01-28

Applicant: MICRONICS INC

Inventor： BATTRELL C FREDERICK ,  SPRAGUE ISAAC ,  BRAGD MATTHEW SCOTT ,  CAPODANNO JASON

IPC: B01L3/00

Abstract: A microfluidic cartridge and methods for performing a diagnostic, molecular or biochemical assay thereon, where all dried and/or liquid reagents necessary for the assay are contained in the cartridge and the assay requires only the addition of sample. Pneumohydraulic features, chamber and diaphragm technologies are introduced for overcoming the problems of bubble interference and reagent washout during operation of a microfluidic cartridge. The cartridges are inserted into a host instrument for performance of an assay and the cartridge is supplied as a consumable.

公开(公告)号：WO2010144683A3

公开(公告)日：2011-02-03

申请号：PCT/US2010038141

申请日：2010-06-10

Applicant: MICRONICS INC ,  BATTRELL C FREDERICK ,  HOEKSTRA DENISE MAXINE ,  HAAB JOAN ,  WILLIFORD JOHN R ,  GATES SABRINA N ,  LEBER ELIZABETH R ,  SPRAGUE ISAAC

Inventor： BATTRELL C FREDERICK ,  HOEKSTRA DENISE MAXINE ,  HAAB JOAN ,  WILLIFORD JOHN R ,  GATES SABRINA N ,  LEBER ELIZABETH R ,  SPRAGUE ISAAC

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification.

Abstract translation: 描述制造方法和组合物用于生产具有用于分子生物学测试的载体试剂的自含微流控盒装置。 敏感试剂以干燥形式储存而不进行冻干或冷冻，并在使用时与生物样品或样品洗脱液在使用时重构。 制造方法包括片和卷制造方法，其中将试剂印刷在适当位置并在凝胶玻璃化之前在单个微流体盒内密封。

公开(公告)号：WO2013106458A3

公开(公告)日：2013-11-07

申请号：PCT/US2013020869

申请日：2013-01-09

Applicant: MICRONICS INC

Inventor： BATTRELL C FREDERICK ,  CLEMMENS JOHN ,  SPRAGUE ISAAC

IPC: B01L3/00 ,  B01F11/02

CPC classification number: B01F11/0071 ,  B01L3/50273 ,  B01L2200/04 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/043 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2400/043 ,  B01L2400/0439 ,  B01L2400/0487 ,  B01L2400/049

Abstract: A compact device for operatively coupling a solid planar substrate, for example a glass slide, to a microfluidic circuit and performing a reaction or reactions on organic matter bound to the face of the planar substrate. Typical reactions include binding, staining and/or labeling reactions. In use, a sealed reaction chamber is formed, the chamber enclosing the organic matter and at least a part of the solid substrate. Headspace in the sealed chamber between the solid substrate is generally of microfluidic dimensions, and diaphragm pump members are used to inject, exchange and/or mix the fluids in the chamber.

Abstract translation: 用于将固体平面基底（例如载玻片）可操作地耦合到微流体回路并且对结合到平面基底的面上的有机物质执行一个或多个反应的紧凑装置。 典型的反应包括结合，染色和/或标记反应。 在使用中，形成密封的反应室，该室封闭有机物质和至少一部分固体基质。 固体基质之间的密封室中的顶空通常具有微流体尺寸，隔膜泵构件用于注入，交换和/或混合室内的流体。

公开(公告)号：CA2786569C

公开(公告)日：2019-04-09

申请号：CA2786569

申请日：2011-01-28

Applicant: MICRONICS INC

Inventor： BATTRELL C FREDERICK ,  SPRAGUE ISAAC ,  BRAGD MATTHEW SCOTT ,  CAPODANNO JASON

IPC: B01L3/00

Abstract: A microfluidic cartridge and methods for performing a diagnostic, molecular or biochemical assay thereon, where all dried and/or liquid reagents necessary for the assay are contained in the cartridge and the assay requires only the addition of sample. Pneumohydraulic features, chamber and diaphragm technologies are introduced for overcoming the problems of bubble interference and reagent washout during operation of a microfluidic cartridge. The cartridges are inserted into a host instrument for performance of an assay and the cartridge is supplied as a consumable.

公开(公告)号：EP2934751A4

公开(公告)日：2016-11-16

申请号：EP13865758

申请日：2013-12-20

Applicant: MICRONICS INC

Inventor： SPRAGUE ISAAC ,  KAY JUSTIN L ,  BRAGD MATTHEW SCOTT ,  BATTRELL C FREDERICK

IPC: B01L3/00

CPC classification number: B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2400/06 ,  F04B19/006 ,  F04B43/043 ,  F16K99/0015 ,  F16K99/0055 ,  F16K2099/0084

Abstract: Microfluidic circuit elements, such as a microvalve, micropump or microvent, formed of a microcavity divided by a diaphragm web into a first subcavity bounded by a first internal wall and a second subcavity bounded by a second internal wall, where the diaphragm web is characterized as a thin film having a first state contacting the first internal wall and a second state contacting the second internal wall and exhibiting essentially no elasticity in moving between the first state and the second state, the thin film web having been stretched beyond its yield point before or during use are provided. The disclosed elements enable faster and more efficient cycling of the diaphragm in the microcavity and increases the diaphragm surface area. In a preferred embodiment, the microfluidic circuit element is pneumatically driven and controls the motion of fluids in a microassay device.

Abstract translation: 微流体电路元件，例如微阀，微型泵或微量元件，由微腔分隔成由第一内壁限定的第一子空腔和由第二内壁限定的第二子腔，微腔由隔膜腹板分隔，其中隔膜腹板的特征在于 具有接触第一内壁的第一状态的薄膜和接触第二内壁的第二状态，并且在第一状态和第二状态之间基本上没有弹性移动，薄膜纤维网已被拉伸超过其屈服点之前或 在使用期间提供。 所公开的元件使得隔膜在微腔中更快且更有效地循环并增加隔膜表面积。 在优选实施例中，微流体电路元件被气动驱动并且控制微量测量装置中的流体的运动。

公开(公告)号：EP2935908A4

公开(公告)日：2016-11-23

申请号：EP13865265

申请日：2013-12-20

Applicant: MICRONICS INC

Inventor： KOLB ANDREW ,  SPRAGUE ISAAC ,  KAY JUSTIN L ,  BRAGD MATTHEW SCOTT ,  STONE MARTHA

IPC: F15C3/04

CPC classification number: B01L3/50273 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  B29C43/021 ,  B29C65/16 ,  B29K2101/00 ,  B29L2009/00 ,  B29L2031/7496 ,  B81B2201/052 ,  B81C1/00119 ,  F04B43/043 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0086 ,  Y10T156/1026

Abstract: Fluidic cartridges, and manufacture thereof, having a plurality of circuit element subtypes containing pneumatically operated diaphragm members, where the diaphragm materials are selected for yield point, chemical resistance, breathability and other properties individually according to the fluidic element subtype are provided. A process of in-situ edge-bonded decoupage for forming diaphragm members inside a cartridge, and fluidic circuits having diaphragm members as active and passive circuit elements, including pumps, valves, vents, waste receptacles, reagent reservoirs, and cuvettes with optical windows, where the material composition of each individual diaphragm member may be selected from an assortment of materials during manufacture are also provided.

Abstract translation: 提供具有包含气动操作的隔膜部件的多个电路元件亚型的流体盒及其制造，其中根据流体元件亚型分别选择隔膜材料用于屈服点，耐化学性，透气性和其它性质。 用于在筒内形成隔膜构件的原位边缘粘合去耦的过程，以及具有作为主动和被动电路元件的隔膜构件的流体回路，包括泵，阀，通风口，废物容器，试剂储存器和具有光学窗口的比色杯， 其中还提供了每个单独隔膜构件的材料组成可以在制造期间从各种材料中选择。