公开(公告)号：US20060103841A1

公开(公告)日：2006-05-18

申请号：US11211619

申请日：2005-08-26

Applicant: Kazushi Ohishi ,  Hiroshi Ohta

Inventor： Kazushi Ohishi ,  Hiroshi Ohta

IPC: G01J3/28

CPC classification number: G01J3/1256 ,  G01J3/02 ,  G01J3/0205 ,  G01J3/0224 ,  G01J3/06 ,  G01J3/1804 ,  G01J2003/064

Abstract: An object of the invention is to realize an optical spectrum analyzer capable of performing high-speed waveform sweep. The invention is to make improvements to an optical spectrum analyzer for measuring a spectrum of light to be measured by collimating light to be measured by collimator means, spectroscopically separating the collimated light incident from the collimator means according to an incident angle by a diffraction grating, and detecting the light spectroscopically separated by the diffraction grating by a photodetector via a slit. The device is characterized by including an acoustooptic deflector provided between the collimator means and the diffraction grating for deflecting the collimated light to be measured and changing the incident angle on the diffraction grating.

Abstract translation: 本发明的目的是实现能够执行高速波形扫描的光谱分析仪。 本发明是为了对光谱分析仪进行改进，用于通过准直器准备待测光来测量待测光的光谱，通过衍射光栅根据入射角光谱分离入射到准直器装置的准直光， 并且通过光栅检测由光电检测器通过狭缝光谱地被衍射光栅分离的光。 该装置的特征在于包括设置在准直器装置和衍射光栅之间的声光偏转器，用于偏转待测量的准直光并改变衍射光栅上的入射角。

公开(公告)号：US20060044556A1

公开(公告)日：2006-03-02

申请号：US11209693

申请日：2005-08-24

Applicant: Yoshihiro Kawano

Inventor： Yoshihiro Kawano

IPC: G01J3/30 ,  G01N21/64

CPC classification number: G01N21/6456 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/10 ,  G01J3/1256 ,  G01J3/4406 ,  G01J2003/1221 ,  G01J2003/1286 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6463

Abstract: Fluorescence emitted from a tissue can be detected efficiently, and excitation light with a plurality of wavelengths can be simultaneously emitted to simultaneously detect fluorescence with a plurality of wavelengths. A laser-scanning fluoroscopy apparatus includes a laser light source of a plurality of wavelengths; a spectroscopic device for splitting laser beams according to wavelength; a focusing lens for focusing the split laser beams; and a wavelength-selecting reflection device which includes a plurality of reflection sections disposed near the focal positions, spaced out at predetermined intervals in a split direction to reflect the laser beams with different wavelengths and a transmission section arranged adjacent to the reflection sections. The laser-scanning fluoroscopy apparatus further includes a diffraction grating for combining the reflected laser beams; a scanning section for two-dimensionally scanning the combined laser beams; an objective optical system for focusing the scanned laser beams onto a tissue; and a photodetector for detecting fluorescence emitted from the tissue. A width dimension, as measured along the split direction, of each reflection section of the wavelength-selecting reflection device is smaller than a width dimension of the transmission section.

Abstract translation: 可以有效地检测从组织发出的荧光，并且可以同时发射具有多个波长的激发光，以同时检测多个波长的荧光。 激光扫描透视设备包括多个波长的激光光源; 用于根据波长分割激光束的分光装置; 用于聚焦分束激光束的聚焦透镜; 以及波长选择反射装置，其包括设置在聚焦位置附近的多个反射部分，以分割方向以预定间隔隔开以反射具有不同波长的激光束，以及布置在反射部分附近的透射部分。 激光扫描透视装置还包括用于组合反射的激光束的衍射光栅; 用于二维扫描组合的激光束的扫描部分; 用于将扫描的激光束聚焦到组织上的物镜光学系统; 以及用于检测从组织发出的荧光的光电检测器。 波长选择反射装置的每个反射部分沿分割方向测量的宽度尺寸小于透射部分的宽度尺寸。

公开(公告)号：US20050264808A1

公开(公告)日：2005-12-01

申请号：US10967075

申请日：2004-10-15

Applicant: Xiaomei Wang

Inventor： Xiaomei Wang

IPC: G01J3/06 ,  G01J3/12 ,  G01J3/18 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01N21/39 ,  G01N21/65

CPC classification number: G01J3/18 ,  G01J3/0256 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/44 ,  G01J2003/068 ,  G01J2003/1247 ,  G01N21/39 ,  G01N21/65

Abstract: A spectrometer that provides the ability to combine the advantages of high resolution, compactness, ruggedness, and low-power consumption of Fabry-Perot (FP) tunable filter spectrometer, with the multi-channel multiplexing advantage of FT and/or grating/detector array. The key concept is to design and operate a tunable FP filter in a multiple-order condition. This filter is then followed by a “low-resolution” fixed grating, which disperses the filtered n-order signal into a preferably matched N-element detector array for parallel detection. The spectral resolution in this system is determined by the FP filter, which can be designed to have very high resolution. The N-order parallel detection scheme reduces the total integration or scan time by a factor of N to achieve the same signal to noise ratio (SNR) at the same resolution as the single channel tunable filter method. This design is also very flexible, allowing spectrometer systems with appropriate order N to thereby optimize the system performance for spectral resolution and scan integration time. In addition to the significant reduction in scan integration time, there are two other advantages to this approach. The first, because the FP tunable filter is designed and operated under n-orders, the fabrication tolerances of the FP filter cavity and operating conditions are significantly loosened.

Abstract translation: 光谱仪提供了结合Fabry-Perot（FP）可调谐滤光片的高分辨率，紧凑性，坚固性和低功耗优点的能力，以及FT和/或光栅/检测器阵列的多通道复用优势 。 关键概念是设计和操作多阶可调FP滤波器。 该滤波器之后是“低分辨率”固定光栅，其将滤波后的n阶信号分散到优选匹配的N元件检测器阵列中用于并行检测。 该系统中的光谱分辨率由FP滤光片确定，可以设计成具有非常高的分辨率。 N级并行检测方案将总积分或扫描时间减少N倍，以与单通道可调滤波器方法相同的分辨率实现相同的信噪比（SNR）。 该设计也非常灵活，允许光谱仪系统具有适当的次序N，从而优化系统性能，以实现光谱分辨率和扫描积分时间。 除了显着降低扫描积分时间之外，还有另外两个优点。 首先，由于FP可调谐滤波器是按n阶设计和操作的，FP滤波器腔的制造公差和工作条件明显松动。

公开(公告)号：US06965431B2

公开(公告)日：2005-11-15

申请号：US10376965

申请日：2003-02-28

Applicant: Tuan Vo-Dinh ,  Alan Wintenberg

Inventor： Tuan Vo-Dinh ,  Alan Wintenberg

IPC: G01J3/12 ,  G01J3/28 ,  G01J3/44 ,  G01N21/65 ,  G01V3/44

CPC classification number: G01J3/44 ,  G01J3/1256 ,  G01J3/2889 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/656

Abstract: A scanning tunable detection system and related method for analyzing samples includes a source of time varying excitation signals and a tunable optical filter for selectively transmitting time-varying optical signals emanated from a sample following irradiation with the time varying excitation signals. A detector is provided for converting the time-varying optical signals to electrical detection signals. The system can identify components in a sample using phase sensitive or time sensitive detection. A slew scan mode can be used to permit slow scanning through spectral regions rich in information but quickly in regions without such information.

Abstract translation: 用于分析样本的扫描可调检测系统和相关方法包括时变激励信号源和可调谐光学滤波器，用于选择性地传输随时间变化的激发信号照射后从样品发出的时变光信号。 提供了用于将时变光信号转换为电检测信号的检测器。 该系统可以使用相敏或时间敏感检测来识别样品中的成分。 可以使用旋转扫描模式来允许在没有这种信息的区域中快速扫描富含信息的频谱区域，但是快速地扫描。

公开(公告)号：US06687007B1

公开(公告)日：2004-02-03

申请号：US09736916

申请日：2000-12-14

Applicant: Andrew D. Meigs

Inventor： Andrew D. Meigs

IPC: G01B902

CPC classification number: G01J3/453 ,  G01J3/0205 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/1256 ,  G01J3/2889

Abstract: Optical instruments having, inter alia, optics to process wavelengths of electromagnetic radiation to produce an interferogram. The instruments include at least one optical path and optical elements positioned along this path for splitting the electromagnetic radiation and spectrally dispersing the wavelengths to produce first and second sets of spectrally dispersed beams which interfere with each other to produce a plurality of different fringes of different wavelengths. The optics for dispersing the wavelengths includes a matched pair of gratings. The gratings may be reflective or they may be transmissive. The optics also includes a beam splitter and first and second mirrors. The gratings may be positioned in a variety of locations along the optical path. The instruments can also include a detector for detecting the interferogram and means for processing the detected interferogram to produce spectral information.

Abstract translation: 光学仪器尤其具有用于处理电磁辐射波长以产生干涉图的光学装置。 这些仪器包括至少一个光路和沿该路径定位的光学元件，用于分离电磁辐射并光谱地分散波长，以产生彼此干涉以产生不同波长的多个不同条纹的第一和第二组光谱分散光束 。 用于分散波长的光学器件包括一对匹配的光栅。 光栅可以是反射性的或它们可以是透射的。 光学器件还包括分束器和第一和第二反射镜。 光栅可以沿着光路定位在各种位置。 仪器还可以包括用于检测干涉图的检测器和用于处理检测到的干涉图以产生光谱信息的装置。

公开(公告)号：US20030179372A1

公开(公告)日：2003-09-25

申请号：US10393607

申请日：2003-03-21

Applicant: Leica Microsystems Heidelberg GmbH

Inventor： Werner Knebel

IPC: G01N021/64

CPC classification number: G01N21/6458 ,  G01J3/1256 ,  G01J3/32 ,  G01J3/36 ,  G01N2021/6421 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/16 ,  G02B21/365

Abstract: A method for spectral analysis of the light proceeding from a specimen using a multi-band detector comprises the steps of defining an overall spectral region; from the overall spectral region, defining a first spectral subregion and defining at least a second spectral subregion; simultaneously detecting the light proceeding from the specimen in the first and the second spectral subregion, and generating detection values; displacing the first spectral subregion and displacing the second spectral subregion within the overall spectral region; and repeating steps c) and d) until the light has been detected over the entire overall spectral region.

Abstract translation: 用于使用多频带检测器从样本进行光的光谱分析的方法包括以下步骤：定义整个光谱区域; 从整个光谱区域，定义第一光谱子区域并限定至少第二光谱子区域; 同时检测从第一和第二频谱子区域中的样本进行的光，并产生检测值; 移位所述第一频谱子区域并且移位所述整个频谱区域内的所述第二频谱子区域; 并重复步骤c）和d），直到在整个光谱区域上检测到光。

公开(公告)号：US06624889B1

公开(公告)日：2003-09-23

申请号：US10135256

申请日：2002-04-29

Applicant: Shifang Li

Inventor： Shifang Li

IPC: G01J400

CPC classification number: G01J3/1256 ,  G01J3/447 ,  H04J14/02 ,  H04J14/06

Abstract: An optical channel monitor (OCM) or filter for analyzing an incident light carrying a number of narrow band signal channels such as WDM or DWDM channels. The OCM or filter use an acousto-optic tunable filter to receive and refract from an incident light a refracted light such that the refracted light contains a test channel with a center frequency &ngr;0. A first birefringent element is provided for filtering from the refracted light a first polarized light and a second polarized light orthogonal to the first polarized light. The transmission curves are engineered such that the transmissions of the first and second polarized light are substantially equal at the center frequency &ngr;0 of the test channel. The OCM or filter has a second birefringent element for filtering from the first polarized light a first polarized portion and a second polarized portion. The transmission curves of the second birefringent element are set such that the transmissions of the first and second polarized portions are substantially equal at a first offset &dgr;1&ngr; from the center frequency &ngr;0.

Abstract translation: 用于分析携带许多窄带信号通道（如WDM或DWDM通道）的入射光的光通道监视器（OCM）或滤波器。 OCM或滤波器使用声光可调谐滤波器从入射光接收和折射折射光，使得折射光包含具有中心频率nu0的测试通道。 提供了第一双折射元件，用于从折射光中滤出与第一偏振光正交的第一偏振光和第二偏振光。 传输曲线被设计成使得第一和第二偏振光的传输在测试通道的中心频率nu0处基本相等。 OCM或滤波器具有用于从第一偏振光第一偏振部分和第二偏振部分滤波的第二双折射元件。 第二双折射元件的透射曲线被设定为使得第一和第二偏振部分的透射在与中心频率nu0相距的第一偏移量Δ1nu处基本相等。

公开(公告)号：US6007996A

公开(公告)日：1999-12-28

申请号：US122704

申请日：1998-07-27

Applicant: George McNamara ,  Dirk Soenksen ,  Dario Cabib ,  Robert A. Buckwald

Inventor： George McNamara ,  Dirk Soenksen ,  Dario Cabib ,  Robert A. Buckwald

IPC: G01B11/00 ,  A61B3/00 ,  A61B3/12 ,  A61B5/00 ,  A61B10/00 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/443 ,  G01J3/45 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G02B21/36 ,  G06K9/00 ,  G06K9/76 ,  G01N33/53

CPC classification number: G01N21/6458 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6423

Abstract: A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.

Abstract translation: 一种生物样品的原位分析方法，包括以下步骤：（a）染色具有N染色的生物样品，其中第一染色选自第一免疫组织化学染色，第一组织染色和第一DNA倍体 染色，第二染色选自第二免疫组织化学染色，第二组织学染色和第二DNA倍性染色，其规定N为大于3的整数，并且（i）如果第一染色是 第一次免疫组织化学染色，第二次染色是第二次组织染色或第二次DNA倍体染色; （ii）如果第一染色是第一组织染色，则第二染色是第二免疫组织化学染色或第二DNA倍体染色; 而（iii）如果第一个染色是第一个DNA倍性染色，则第二次染色是第二次免疫组化染色或第二次组织染色; 和（b）使用用于收集来自生物样品的光谱数据的光谱数据采集装置，选择光谱数据采集装置和N染色，使得与每个N染色体相关联的光谱分量是可收集的。

公开(公告)号：US5936731A

公开(公告)日：1999-08-10

申请号：US575191

申请日：1995-12-20

Applicant: Dario Cabib ,  Robert A. Buckwald ,  David Wine ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen ,  Moshe Lavi

Inventor： Dario Cabib ,  Robert A. Buckwald ,  David Wine ,  Yuval Garini ,  Nir Katzir ,  Dirk G. Soeknsen ,  Moshe Lavi

IPC: G01B9/02 ,  A61B3/12 ,  A61B5/00 ,  C12Q1/68 ,  G01B11/06 ,  G01J3/02 ,  G01J3/12 ,  G01J3/26 ,  G01J3/28 ,  G01J3/44 ,  G01J3/453 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58 ,  G06K9/00 ,  G06K9/76

CPC classification number: G01N21/6458 ,  A61B3/12 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6883 ,  G01B11/0675 ,  G01J3/12 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J3/4535 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G06K9/00127 ,  G06K9/76 ,  A61B3/1241 ,  C12Q2600/156 ,  G01J2003/2866 ,  G01J3/02 ,  G01N2021/6417 ,  G01N2021/6441

Abstract: A fluorescent in situ hybridization method comprising the steps of (a) providing a cell nuclei having chromosomes hybridized with at least one nucleic acid probe including at least one nucleic acid molecule labeled with at least one fluorophore; (b) viewing the cell nuclei through a fluorescence microscope optically connected to an imaging spectrometer for obtaining a spectrum of each pixel of the cell nuclei by (i) collecting incident collimated light simultaneously from all pixels of the cell nuclei; (ii) passing the incident collimated light through an interferometer system so that the light is first split into two coherent beams and then recombine to interfere and form an exiting light beam; (iii) focusing the exiting light beam on a detector having an array of detector elements, so that at each instant each of the elements is the image of one and always the same pixel for the entire duration of the measurement and so that each of the elements produces a signal which is a particular linear combination of light intensity emitted by the pixel at different wavelengths; (iv) rotating or translating one or more of the elements of the interferometer, so that the optical path difference is scanned simultaneously for all the pixels of the cell nuclei; and (v) recording signals of each of the detector elements as function of time using a recording device to form a first spectral cube of data; and (c) interpreting the first spectral cube of data using a mathematical algorithm.

Abstract translation: 荧光原位杂交方法包括以下步骤：（a）提供具有染色体的细胞核，所述染色体与至少一种核酸探针杂交，所述核酸探针包括至少一个用至少一个荧光团标记的核酸分子; （b）通过光学连接到成像光谱仪的荧光显微镜观察细胞核，通过（i）从细胞核的所有像素同时收集入射准直光，以获得细胞核的每个像素的光谱; （ii）使入射的准直光通过干涉仪系统，使得光首先分裂成两个相干光束，然后重组以干涉并形成出射的光束; （iii）将出射的光束聚焦在具有检测器元件阵列的检测器上，使得在每个时刻，在整个测量持续时间内，每个元件是一个并且总是相同像素的图像，并且使得每个 元件产生信号，其是由不同波长的像素发射的光强度的特定线性组合; （iv）旋转或平移干涉仪的一个或多个元件，使得对于细胞核的所有像素同时扫描光程差; 以及（v）使用记录装置记录每个检测器元件的信号作为时间的函数，以形成数据的第一光谱立方体; 和（c）使用数学算法解释数据的第一个频谱立方体。

公开(公告)号：US5821536A

公开(公告)日：1998-10-13

申请号：US978133

申请日：1997-11-25

Applicant: John W. Pettit

Inventor： John W. Pettit

IPC: G01J3/12 ,  G01N21/35 ,  G01N21/86

CPC classification number: G01J3/1256 ,  G01N21/3559

Abstract: A solid state moisture gauge is disclosed in which a quartz-halogen bulb is used with an acousto-optic tunable filter to launch light of specific desired wavelengths into a parallel plate cavity device which contains or is adjacent to material having a moisture content which is to be measured. The light exiting from the parallel plate device is detected, converted to a digital signal and then stored in a computer for later comparison to the detection of light exiting from the parallel plate device of a different wavelength.

Abstract translation: 公开了一种固态湿度计，其中石英卤素灯泡与声光可调谐滤光器一起使用以将特定期望波长的光发射到平行板空腔装置中，该平行板腔装置包含或邻近具有水分含量的材料 被测量。 从平行板装置出射的光被检测，转换为数字信号，然后存储在计算机中，以便与从不同波长的平行板装置出射的光的检测进行比较。