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公开(公告)号：US11193875B2
公开(公告)日：2021-12-07
申请号：US15581395
申请日：2017-04-28
Applicant: Abbott Laboratories
Inventor： Martin Krockenberger , Jiong Wu , Bodo Roemer , Giacomo Vacca
Abstract: A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.
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公开(公告)号：US20210123912A1
公开(公告)日：2021-04-29
申请号：US16998776
申请日：2020-08-20
Applicant: Abbott Laboratories
Inventor： Jiong Wu
IPC: G01N33/569 , G01N15/14
Abstract: Aspects of the invention include WBC analysis reagents, systems and methods that can be used for analyzing a sample of whole blood to identify, classify, and/or quantify white blood cells (WBC) and WBC sub-populations in the sample. The WBC analysis reagents of the present disclosure generally include at least one membrane-permeable fluorescent dye, a WBC protecting reagent, and a surfactant. In some embodiments, the WBC reagents include a suitable amount of an osmolality adjusting component to adjust the osmolality of the WBC reagent into a desired range.
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公开(公告)号：US20200309802A1
公开(公告)日：2020-10-01
申请号：US16849534
申请日：2020-04-15
Applicant: Abbott Laboratories
Inventor： Roger W. Accurso , Jiong Wu , Richard G. Kendall , James A. Janik , Ronald J. Shanafelter , Robert Fahey , Svitlana Y. Berezhna , Trevor David Shields , Mahmoud Janbakhsh , Daryl Wong , Jorge Haro , Michael J. Kennedy
Abstract: Aspects of the present disclosure include systems and methods. According to certain embodiments, provided is an integrated analysis system that includes a first module including a sample analysis component and a first internal container conveyor system. The integrated analysis system further includes a second module including a second internal container conveyor system. The first and second modules are positioned adjacent each other such that the first and second internal container conveyor systems are aligned and adapted to transport containers from the first module to the second module. Also provided are methods of analyzing and preparing samples (e.g., blood and body fluid samples), as well as components that find use within the analysis systems of the present disclosure.
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公开(公告)号：US10775379B2
公开(公告)日：2020-09-15
申请号：US14137309
申请日：2013-12-20
Applicant: Abbott Laboratories
Inventor： Jiong Wu
IPC: G01N33/569 , G01N15/14 , G01N15/10 , G01N15/00
Abstract: Aspects of the invention include WBC analysis reagents, systems and methods that can be used for analyzing a sample of whole blood to identify, classify, and/or quantify white blood cells (WBC) and WBC sub-populations in the sample. The WBC analysis reagents of the present disclosure generally include at least one membrane-permeable fluorescent dye, a WBC protecting reagent, and a surfactant. In some embodiments, the WBC reagents include a suitable amount of an osmolality adjusting component to adjust the osmolality of the WBC reagent into a desired range.
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公开(公告)号：US20140273060A1
公开(公告)日：2014-09-18
申请号：US14137309
申请日：2013-12-20
Applicant: Abbott Laboratories
Inventor： Jiong Wu
IPC: G01N33/50
Abstract: Aspects of the invention include WBC analysis reagents, systems and methods that can be used for analyzing a sample of whole blood to identify, classify, and/or quantify white blood cells (WBC) and WBC sub-populations in the sample. The WBC analysis reagents of the present disclosure generally include at least one membrane-permeable fluorescent dye, a WBC protecting reagent, and a surfactant. In some embodiments, the WBC reagents include a suitable amount of an osmolality adjusting component to adjust the osmolality of the WBC reagent into a desired range.
Abstract translation: 本发明的方面包括可以用于分析全血样品以识别，分类和/或定量样品中的白细胞（WBC）和WBC亚种群的WBC分析试剂，系统和方法。 本公开的WBC分析试剂通常包括至少一种透膜荧光染料，WBC保护试剂和表面活性剂。 在一些实施方案中，WBC试剂包括适量的渗透压浓度调节组分以将WBC试剂的重量摩尔渗透压浓度调节至所需范围。
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Patent Agency Ranking
公开(公告)号：US20130164740A1
公开(公告)日：2013-06-27
申请号：US13720615
申请日：2012-12-19
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Emily H. Lin , Jihping Yang
IPC: C12Q1/04
CPC classification number: C12Q1/04 , G01N33/5094
Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
Abstract translation: 本文提供了一种简单，可靠和准确的血液分析仪细胞分析方法。 在各个方面，所述方法通过利用荧光染料选择性地染色WBCs使得它们发射更强的荧光信号来提供红细胞（RBC）和白细胞（WBC）之间的分离和/或分化。 该方法提供了对WBC和RBCs的最佳检测限，从而允许对具有稀疏细胞浓度的样品进行分析。 为了简化体液分析，可以使用少量一种试剂来制备用于体液分析的单一稀释液。 使用本公开内容中描述的无裂解方法获得对WBC的最小损伤。
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公开(公告)号：US20210130866A1
公开(公告)日：2021-05-06
申请号：US17002089
申请日：2020-08-25
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Emily H. Lin , Jihping Yang
Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
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公开(公告)号：US10481072B2
公开(公告)日：2019-11-19
申请号：US15704974
申请日：2017-09-14
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Marilou Coleman , Emily H. Lin , Michael R. Buhl , Giacomo Vacca
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
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公开(公告)号：US09970045B2
公开(公告)日：2018-05-15
申请号：US13720615
申请日：2012-12-19
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Emily H. Lin , Jihping Yang
CPC classification number: C12Q1/04 , G01N33/5094
Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
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公开(公告)号：US20180128732A1
公开(公告)日：2018-05-10
申请号：US15704974
申请日：2017-09-14
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Marilou Coleman , Emily H. Lin , Michael R. Buhl , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/147 , G01N21/6428 , G01N33/49 , G01N33/80 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
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