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公开(公告)号：US11287382B2
公开(公告)日：2022-03-29
申请号：US16689004
申请日：2019-11-19
Applicant: Quantum-Si Incorporated
Inventor： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid , Benjamin Cipriany , Jack Jewell , Lawrence C. West , Michael Ferrigno , Paul E. Glenn , Adam Ezra Cohen , Anthony Bellofiore
IPC: G01N21/64 , C12Q1/6874 , G01N21/77 , B01L3/00 , C12Q1/6869
Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.
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公开(公告)号：US11237326B2
公开(公告)日：2022-02-01
申请号：US16042968
申请日：2018-07-23
Applicant: Quantum-Si Incorporated
Inventor： Jonathan M. Rothberg , Gerard Schmid , Alexander Gondarenko , James Beach , Kyle Preston , Farshid Ghasemi , Jeremy Lackey , Jack Jewell , Keith G. Fife , Ali Kabiri
Abstract: An integrated device and related instruments and systems for analyzing samples in parallel are described. The integrated device may include sample wells arranged on a surface of where individual sample wells are configured to receive a sample labeled with at least one fluorescent marker configured to emit emission light in response to excitation light. The integrated device may further include photodetectors positioned in a layer of the integrated device, where one or more photodetectors are positioned to receive a photon of emission light emitted from a sample well. The integrated device further includes one or more photonic structures positioned between the sample wells and the photodetectors, where the one or more photonic structures are configured to attenuate the excitation light relative to the emission light such that a signal generated by the one or more photodetectors indicates detection of photons of emission light.
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13.发明授权公开(公告)号：US11224878B2
公开(公告)日：2022-01-18
申请号：US15971493
申请日：2018-05-04
Applicant: Quantum-Si Incorporated
Inventor： Jonathan M. Rothberg , Jeremy Lackey , Guojun Chen
IPC: B01L3/00 , C12Q1/6848 , C09D5/16 , C09D187/00 , C09D171/02
Abstract: Methods of preparing surfaces of sample wells are provided. In some aspects, methods of preparing a sample well surface involve contacting the sample well with a block copolymer to form an antifouling overlay over a metal oxide surface of the sample well. In some aspects, methods of passivating and/or selectively functionalizing a sample well surface are provided.
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公开(公告)号：US20200209257A1
公开(公告)日：2020-07-02
申请号：US16709077
申请日：2019-12-10
Applicant: Quantum-Si Incorporated
Inventor： Brian Reed , Jeremy Lackey , Thomas Christian , Roger Nani , David Dodd , Kathren Croce , Alexander Goryaynov , Jonathan M. Rothberg
Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
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公开(公告)号：US20190211389A1
公开(公告)日：2019-07-11
申请号：US16239789
申请日：2019-01-04
Applicant: Quantum-Si Incorporated
Inventor： Guojun CHEN , Jeremy Lackey , Alex Goryanyov , Gerard Schmid , Ali Kabiri , Jonathan M. Rothberg , Todd Rearick , Jonathan C. Schultz , Farshid Ghasemi , Keith G. Fife
IPC: C12Q1/6869 , C12Q1/6806 , B01L3/00 , C08L1/02 , G01N21/64 , G01N27/447
CPC classification number: C12Q1/6869 , B01L3/502761 , B01L3/50857 , B01L2200/0647 , B01L2300/0645 , B01L2300/0829 , B01L2300/0864 , B01L2400/0415 , B01L2400/0421 , C08L1/02 , C12Q1/6806 , G01N21/6428 , G01N27/44743 , G01N27/44782
Abstract: Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.
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Patent Agency Ranking
16.发明授权公开(公告)号：US10288566B2
公开(公告)日：2019-05-14
申请号：US15845996
申请日：2017-12-18
Applicant: Quantum-Si Incorporated
Inventor： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid , Lawrence C. West , Keith G. Fife , Benjamin Cipriany , Farshid Ghasemi
IPC: G01N21/64 , C12Q1/6874 , C12Q1/6869
Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes at least one waveguide configured to propagate excitation energy to the sample wells from a region of the integrated device configured to couple with an excitation energy source. A pixel may also include at least one element for directing the emission energy towards a sensor within the pixel. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device. One of multiple markers distinguishable by temporal parameters of the emission energy may label the sample and configuration of the sensor within a pixel may allow for detection of a temporal parameter associated with the marker labeling the sample.
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公开(公告)号：US20180362941A9
公开(公告)日：2018-12-20
申请号：US15846967
申请日：2017-12-19
Applicant: Quantum-Si Incorporated
Inventor： Brian Reed , Mohammad Wadud Bhuiya , Manjula Pandey , Jeremy Lackey , Jonathan M. Rothberg
IPC: C12N9/12 , C12Q1/6869
Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.
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18.发明申请公开(公告)号：US20180348132A1
公开(公告)日：2018-12-06
申请号：US15960751
申请日：2018-04-24
Applicant: Quantum-Si Incorporated
Inventor： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid
IPC: G01N21/64 , B01L3/00 , C12Q1/6869 , C12Q1/6874 , G01N21/77
Abstract: An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.
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19.发明授权公开(公告)号：US09885657B2
公开(公告)日：2018-02-06
申请号：US14821688
申请日：2015-08-07
Applicant: Quantum-Si Incorporated
Inventor： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid , Lawrence C. West , Keith G. Fife , Benjamin Cipriany , Farshid Ghasemi
CPC classification number: G01N21/6408 , C12Q1/6869 , C12Q1/6874 , G01N21/6428 , G01N21/6454 , G01N21/648 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6463 , G01N2201/0696 , G01N2201/08 , G01N2201/12
Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes at least one waveguide configured to propagate excitation energy to the sample wells from a region of the integrated device configured to couple with an excitation energy source. A pixel may also include at least one element for directing the emission energy towards a sensor within the pixel. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device. One of multiple markers distinguishable by temporal parameters of the emission energy may label the sample and configuration of the sensor within a pixel may allow for detection of a temporal parameter associated with the marker labeling the sample.
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公开(公告)号：US12259324B2
公开(公告)日：2025-03-25
申请号：US18356150
申请日：2023-07-20
Applicant: Quantum-Si Incorporated
Inventor： Jonathan M. Rothberg , Ali Kabiri , Gerard Schmid , Keith G. Fife , James Beach , Jason W. Sickler , Lawrence C. West , Paul E. Glenn , Kyle Preston , Farshid Ghasemi , Benjamin Cipriany , Jeremy Lackey
IPC: G01N21/64 , C12Q1/6869 , C12Q1/6874 , G01N21/77 , G01N33/543
Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device may include multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes a surface having a trench region recessed from a portion of the surface and an array of sample wells, disposed in the trench region. The integrated device also includes a waveguide configured to couple excitation energy to at least one sample well in the array and positioned at a first distance from a surface of the trench region and at a second distance from the surface in a region separate from the trench region. The first distance is smaller than the second distance. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device.
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