公开(公告)号：US20220297031A1

公开(公告)日：2022-09-22

申请号：US17608940

申请日：2020-05-12

Applicant: Puridify Ltd

Inventor： Shameer Subratty ,  Klaus Gebauer ,  Anindya Sengupta ,  Anji Venna ,  Hannes Hjorter ,  Adam Pinnock ,  Ralph Stankowski

IPC: B01D15/22 ,  B01D15/26 ,  B01D15/18 ,  B01J20/26 ,  B01J20/28 ,  B01J20/285

Abstract: A chromatography device (1; 101) comprising: —at least one chromatography material unit (3), wherein said chromatography material unit comprises a convection-based chromatography material; —at least one fluid distribution system (7) which is configured to distribute fluid into and out from the at least one chromatography material unit (3); —an inlet (15); —at least one inlet fluid channel (17a, 17b) connecting the inlet (15) with each chromatography material unit (3) via the fluid distribution system (7); —an outlet (19); and —at least one outlet fluid channel (21) connecting the outlet (19) with each chromatography material unit (3) via the fluid distribution system (7), wherein at least some parts of said chromatography device (1; 101) are overmolded and sealed together by plastic or elastomer leaving at least the inlet (15) and the outlet (19) open.

公开(公告)号：US11353433B2

公开(公告)日：2022-06-07

申请号：US16327985

申请日：2017-08-25

Applicant: Puridify Ltd.

Inventor： Michael Williams ,  Iwan Roberts ,  Oliver Hardick ,  Matthew Townsend ,  Christopher Morris ,  Shameer Subratty

IPC: G01N30/38 ,  G01N30/60 ,  G01N30/84

Abstract: A chromatography system for at least one of tangential flow chromatography and lateral flow chromatography comprising: an inlet; a functionalised adsorbent chromatography medium downstream of the inlet; an outlet downstream of the adsorbent chromatography medium; and a flow guide downstream of the inlet and upstream of the adsorbent chromatography medium and configured to distribute a flow of a liquid from the inlet across a width of the adsorbent chromatography medium; wherein the flow guide comprises a pattern of channels providing flow paths from the inlet to different parts of the adsorbent chromatography medium along the width of the adsorbent chromatography medium, wherein the pattern of channels is provided so as to reduce a difference in arrival time and/or flow velocity of liquid reaching the adsorbent chromatography medium across the width of the adsorbent chromatography medium.

公开(公告)号：USD951477S1

公开(公告)日：2022-05-10

申请号：US29756775

申请日：2020-10-30

Applicant: Puridify Ltd

Designer： Shameer Subratty ,  Adam Pinnock ,  Hannes Hjorter ,  Lars Rosengren ,  Anji Reddy Venna ,  Nirmal Kumar Thanikachalam ,  Gabriel Zenkner

公开(公告)号：US11103853B2

公开(公告)日：2021-08-31

申请号：US16314943

申请日：2017-07-14

Applicant: Puridify Ltd.

Inventor： Ian Scanlon

IPC: B01J20/285 ,  A61P35/00 ,  A61K48/00 ,  B01J20/28 ,  C12N7/00 ,  C12N7/02

Abstract: The present invention provides a process for recovering a viral product from a composition comprising said product and product-related impurities, which process comprises contacting the composition with a functionalised chromatography medium comprising one or more polymer nanofibres, wherein the viral product comprises a plurality of viruses, virus particles/virions, viral cores, membrane-stripped viruses, viral cores with outer membrane(s) removed and/or capsids removed, or proviruses, each of which contains one or more polynucleotides, and wherein the product-related impurities comprise a plurality of viruses, virus particles/virions, virus-like particles, viral cores, membrane-stripped viruses, viral cores with outer membrane(s) removed and/or capsids removed or proviruses, each of which is substantially devoid of polynucleotides.

公开(公告)号：US11097252B2

公开(公告)日：2021-08-24

申请号：US16315088

申请日：2017-07-14

Applicant: Puridify Ltd.

Inventor： Marc Hummersone ,  Benjamin Wallis ,  Frederic Leroux ,  William Law

IPC: B01D15/20 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/286 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07K1/16

Abstract: The present invention provides a functionalised polymeric chromatography medium, prepared by a process which comprises (i) providing a substrate formed of one or more polymer nanofibres, (ii) grafting one or more neutral polymer chains from the substrate, and (iii) contacting the grafted product with a reagent which functionalises the product of step (ii) as a chromatography medium, wherein step (ii) comprises reacting a plurality of compounds of formula and/or its enantiomers, and/or its derivatives of formula (I) and/or enantiomers and/or diastereomers thereof: with one or more functional groups present on the nanofibre substrate, wherein R1, R2, R3, R4 and R5 may be the same or different, and are chosen from H, halogen, C1-C4 alkyl, or C1-C4 alkoxy provided that at least one of R1, R2, R3, R4 or R5 is not hydrogen.

公开(公告)号：US11008364B2

公开(公告)日：2021-05-18

申请号：US16909678

申请日：2020-06-23

Applicant: Puridify Ltd.

Inventor： Daniel Gilbert Bracewell ,  Robert Stevens ,  Oliver Hardick

IPC: C07K1/16 ,  B01D15/36 ,  B01J20/28 ,  B01J20/30 ,  C07K1/18 ,  C07K1/22 ,  B01J20/24 ,  B82Y30/00 ,  B01D15/18 ,  B01D15/38 ,  B01J20/285

Abstract: The present invention provides a chromatography medium comprising one or more electrospun polymer nanofibres which in use form a stationary phase comprising a plurality of pores through which a mobile phase can permeate and use of the same.

公开(公告)号：US20210060527A1

公开(公告)日：2021-03-04

申请号：US16959373

申请日：2019-01-07

Applicant: Puridify Ltd.

Inventor： Marc Hummersone ,  Benjamin Wallis ,  Frederic Leroux ,  William Howard Albert Law

IPC: B01J20/26 ,  B01J20/32 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07 ,  B01J20/285 ,  B01D15/32 ,  B01D15/36

Abstract: The invention discloses a functionalised chromatography medium, comprising: i) at least one non-woven layer (10) of polymeric nano fibres (20) comprising a plurality of nanofibre-nano fibre fusion points (30); ii) a grafted polymer coating covering the polymeric nanofibres and the nanofibre-nanofibre fusion points; iii) a plurality of ligand groups covalently bound to the grafted polymer coating, wherein the ligand groups are capable of interacting with a target biomolecule.

公开(公告)号：US10850259B2

公开(公告)日：2020-12-01

申请号：US15091158

申请日：2016-04-05

Applicant: Puridify Ltd.

Inventor： Oliver Hardick ,  Daniel Gilbert Bracewell ,  Stewart Dods

IPC: B01J20/26 ,  B01D15/32 ,  B01J20/24 ,  B01D39/16 ,  B01D15/38 ,  B29C65/02 ,  B32B37/06 ,  C07K1/18 ,  B01J20/285 ,  B01J20/30 ,  B01D15/36 ,  B01J20/28 ,  B01J39/05 ,  B01J41/13 ,  B01J41/07 ,  B01J39/19 ,  B01D39/14 ,  B01J20/287 ,  B01J20/288 ,  B01J39/26 ,  B01J41/20 ,  B29C65/00 ,  B32B5/02 ,  B32B27/08 ,  B32B27/12 ,  B32B27/32 ,  B32B37/10 ,  B32B37/18 ,  C07K1/16 ,  C07K1/20 ,  C07K1/22 ,  C12M1/12 ,  B29K601/00 ,  B29K627/18 ,  B29L9/00

Abstract: The present invention provides a process for preparing a functionalised polymeric chromatography medium, which process comprises (I) providing two or more non-woven sheets stacked one on top of the other, each said sheet comprising one or more polymer nanofibres, (II) simultaneously heating and pressing the stack of sheets to fuse points of contact between the nanofibres of adjacent sheets, and (III) contacting the pressed and heated product with a reagent which functionalises the product of step (II) as a chromatography medium.

公开(公告)号：US20190276491A1

公开(公告)日：2019-09-12

申请号：US16422102

申请日：2019-05-24

Applicant: Puridify Ltd.

Inventor： Daniel Gilbert Bracewell ,  Robert Stevens ,  Oliver Hardick

IPC: C07K1/16 ,  B82Y30/00 ,  B01J20/24 ,  B01D15/38 ,  B01D15/18 ,  B01J20/28 ,  B01D15/36 ,  C07K1/18 ,  B01J20/30 ,  B01J20/285 ,  C07K1/22

Abstract: The present invention provides a chromatography medium comprising one or more electrospun polymer nanofibres which in use form a stationary phase comprising a plurality of pores through which a mobile phase can permeate and use of the same.

公开(公告)号：US20190234914A1

公开(公告)日：2019-08-01

申请号：US16327985

申请日：2017-08-25

Applicant: Puridify Ltd.

Inventor： Michael Williams ,  Iwan Roberts ,  Oliver Hardick ,  Matthew Townsend ,  Christopher Morris ,  Shameer Subratty

IPC: G01N30/38 ,  G01N30/60 ,  G01N30/84

CPC classification number: G01N30/38 ,  G01N30/6017 ,  G01N30/84 ,  G01N2030/386

Abstract: A chromatography system for at least one of tangential flow chromatography and lateral flow chromatography comprising: an inlet; a functionalised adsorbent chromatography medium downstream of the inlet; an outlet downstream of the adsorbent chromatography medium; and a flow guide downstream of the inlet and upstream of the adsorbent chromatography medium and configured to distribute a flow of a liquid from the inlet across a width of the adsorbent chromatography medium; wherein the flow guide comprises a pattern of channels providing flow paths from the inlet to different parts of the adsorbent chromatography medium along the width of the adsorbent chromatography medium, wherein the pattern of channels is provided so as to reduce a difference in arrival time and/or flow velocity of liquid reaching the adsorbent chromatography medium across the width of the adsorbent chromatography medium.