公开(公告)号：US10682640B2

公开(公告)日：2020-06-16

申请号：US15581569

申请日：2017-04-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jiali Liao ,  Russell Frost

IPC: B01J41/20 ,  C07K1/16 ,  B01J20/22 ,  B01D15/30 ,  B01J20/281 ,  B01D15/26 ,  C07K1/14 ,  B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/286 ,  B01J20/289 ,  B01J20/32 ,  B01J41/09 ,  B01J20/26 ,  B01J20/28 ,  B01J20/288 ,  B01J41/04 ,  C07K16/00

Abstract: Solid supports and ligands are provided for purification of biomolecules by mixed-mode anion exchange-hydrophobic chromatography. Compositions can have the formula Support-(X)—N(R1, R2)-R3-L-Ar, or a salt thereof, wherein: Support is a chromatographic solid support; X is a spacer or absent; R1 and R2 are each selected from hydrogen and an alkyl comprising 1-6 carbons; R3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons; L is NR4, O, or S; wherein R4 is hydrogen or an alkyl comprising 1-6 carbons; and Ar is an aryl. Methods are also provided for using solid supports and ligands to purify biomolecules such as monomeric antibodies.

公开(公告)号：US20200086233A1

公开(公告)日：2020-03-19

申请号：US16689677

申请日：2019-11-20

Applicant: W. L. Gore & Associates, Inc.

Inventor： Michael C. McManaway

IPC: B01D15/38 ,  C07K16/00 ,  C07K1/22 ,  B01D69/02 ,  B01D63/10 ,  B01D63/08 ,  B01J20/28 ,  B01J20/10 ,  B01J20/26 ,  B01J20/06 ,  B01D69/14 ,  B01D15/22 ,  B01J20/286

Abstract: The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include at least one polymer membrane that contains therein inorganic particles. The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices may have a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.

公开(公告)号：US10544239B2

公开(公告)日：2020-01-28

申请号：US14966015

申请日：2015-12-11

Applicant: ExxonMobil Research and Engineering Company

Inventor： Quanchang Li ,  David Charles Calabro ,  Mobae Amin Afeworki ,  Simon Christopher Weston ,  Matcheld Maria Wilfried Mertens ,  Tilman Wolfram Beutel ,  Wenyih Frank Lai ,  Paul Podsiadlo ,  Jean Willem Lodewijk Beeckman

IPC: C10G45/52 ,  B01D15/00 ,  C08F10/02 ,  B01J31/06 ,  B01J35/10 ,  C08G77/58 ,  C08G77/50 ,  B01J20/08 ,  B01J20/28 ,  B01J37/03 ,  B01J20/06 ,  B01J20/10 ,  B01J20/26 ,  B01J20/286 ,  B01J20/30 ,  B01J20/32 ,  B01D53/22 ,  B01J23/42 ,  B01J31/12 ,  C01B37/00 ,  C01B39/00 ,  C08G77/60 ,  C08F2/00 ,  C08F2/10 ,  C08F2/42 ,  C10G25/00 ,  C10G35/06 ,  C10G45/00 ,  C10G45/04 ,  C10G45/34 ,  C10G45/44 ,  C10G45/46 ,  C10G45/60 ,  C10G45/64 ,  C10G47/02 ,  C10G50/00 ,  C10M177/00 ,  C10K1/32 ,  B01J29/03 ,  B01D53/04 ,  B01D53/047 ,  B01D53/02 ,  B01J23/44 ,  C10M101/02 ,  C07F7/00 ,  C08F4/659 ,  C08F4/6592

Abstract: Organosilica materials, which are a polymer of at least one independent monomer of Formula [Z1OZ2OSiCH2]3 (I), wherein each Z1 and Z2 independently represent a hydrogen atom, a C1-C4 alkyl group or a bond to a silicon atom of another monomer and at least one other trivalent metal oxide monomer are provided herein. Methods of preparing and processes of using the organosilica materials, e.g., for catalysis etc., are also provided herein.

公开(公告)号：US20190263856A1

公开(公告)日：2019-08-29

申请号：US16348534

申请日：2017-11-14

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Johan Ohman ,  Mats Ander ,  Therese Graner ,  Anneli Jorsback ,  Jinyu Zou

IPC: C07K1/22 ,  B01J20/24 ,  B01J20/285 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38

Abstract: The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.

公开(公告)号：US20190257801A1

公开(公告)日：2019-08-22

申请号：US16310969

申请日：2017-06-21

Applicant: Purdue Research Foundation

Inventor： Mary J. Wirth ,  Alexis Huckabee ,  Rachel Jacobson ,  Tse-Hong Chen

IPC: G01N30/56 ,  B01J20/286 ,  B01J20/32 ,  B01D15/20

Abstract: The present disclosure relates to a chromatographic stationary phase having a uniform polymer density, and related methods. In particular, the present disclosure relates to a method of forming a uniformly dense stationary phase inside a chromatography column.

公开(公告)号：US20190201812A1

公开(公告)日：2019-07-04

申请号：US16311750

申请日：2017-06-20

Applicant: CLEMSON UNIVERSITY

Inventor： R. Kenneth MARCUS ,  LIUWEI JIANG

IPC: B01D15/20 ,  B01J20/26 ,  B01J20/286 ,  B01J20/32 ,  B01J20/30 ,  B01J20/28

CPC classification number: B01D15/20 ,  B01D15/22 ,  B01D15/305 ,  B01D15/361 ,  B01D15/3804 ,  B01D63/02 ,  B01D67/0093 ,  B01D69/082 ,  B01D71/56 ,  B01D2323/38 ,  B01J20/264 ,  B01J20/28023 ,  B01J20/286 ,  B01J20/3078 ,  B01J20/3212 ,  B01J20/3278 ,  B01J20/328 ,  B01J39/26 ,  B01J41/20 ,  B01J47/127 ,  B01J2220/58 ,  G01N33/68

Abstract: Chromatography devices and methods for forming and using the devices are described. The devices include a polyimide-based support phase and a polymer grafted to a surface of the polyimide-based support phase. A microwave-assisted graft polymerization protocol is described to form the polymer at the surface of the support phase. Devices can be utilized in high-efficiency separation of macromolecules such as proteins.

公开(公告)号：US20190169567A1

公开(公告)日：2019-06-06

申请号：US16208163

申请日：2018-12-03

Applicant: Hideaki Sakai

Inventor： Hideaki SAKAI ,  Masa-aki KAKIMOTO ,  Yu SUDO

IPC: C12N5/00 ,  B01J20/32 ,  B01J20/26 ,  B01J20/286 ,  C08L51/00 ,  C08L101/04

Abstract: By using a graft polymer comprising a dendritic polymer with a styrene skeleton and a hydrophilic polymer grafted to a terminal thereof, a temperature-responsive substrate for cell culture having a temperature-responsive surface for cell culture that allows cells to be cultured with high efficiency and which yet allows cultured cells to be exfoliated in a short period of time and with high efficiency by simply changing the temperature of the substrate surface can be prepared conveniently. If this temperature-responsive substrate for cell culture is used, cells obtained from a variety of tissues can be cultured with high efficiency. If this culture method is utilized, cultured cells can be exfoliated intact in a short amount of time with high efficiency. In addition, by using this graft polymer, a wide range of peptides and proteins can also be separated by simply changing the temperature of a chromatographic carrier. This allows for convenient separation procedure and improves the efficiency of separating operations. What is more, the stereoregularity of the dendritic polymer per se may be utilized to enable separation of solutes based on differences in their molecular structures.

公开(公告)号：US20190162720A1

公开(公告)日：2019-05-30

申请号：US16314477

申请日：2017-07-03

Applicant: KAO CORPORATION

Inventor： Tsuyoshi OBA ,  Sayaka NOJI

IPC: G01N33/543 ,  G01N33/536 ,  B01J20/286 ,  B01J20/26

Abstract: A solid phase support capable of orienting the recognition site of an antibody for detection toward a measurement sample side and of immobilizing the antibody in a measurement area is provided. The solid phase support for protein analysis, wherein a first antibody is immobilized on a surface of a substrate, and a second antibody having an affinity to a substance to be analyzed is linked to the first antibody via protein A or G or a variant thereof is provided.

公开(公告)号：US10294306B2

公开(公告)日：2019-05-21

申请号：US15166761

申请日：2016-05-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Achim Knappik ,  Stefan Paschen

IPC: C07K1/22 ,  C07K16/42 ,  B01J20/32 ,  B01D15/38 ,  C07K16/06 ,  B01J20/286

Abstract: Affinity ligands useful for mild elution affinity chromatography, including affinity ligands specific for immunoglobulins M, A, and E, are disclosed as are method of identifying and using such affinity ligands.

公开(公告)号：US10266622B2

公开(公告)日：2019-04-23

申请号：US15526529

申请日：2015-12-11

Applicant: ExxonMobil Research and Engineering Company

Inventor： Matthew W. Holtcamp ,  Matthew S. Bedoya ,  Charles J. Harlan ,  Quanchang Li ,  Machteld M. W. Mertens

IPC: C08F4/76 ,  C08F4/64 ,  C08F10/02 ,  B01J31/06 ,  B01J35/10 ,  C08G77/58 ,  C08G77/50 ,  B01J20/08 ,  B01J20/28 ,  B01D53/04 ,  B01D53/047 ,  B01J20/10 ,  B01D53/02 ,  B01D15/00 ,  B01J37/03 ,  B01J20/06 ,  B01J20/26 ,  B01J20/286 ,  B01J20/30 ,  B01J20/32 ,  B01D53/22 ,  B01J23/42 ,  B01J23/44 ,  B01J31/12 ,  C01B37/00 ,  C01B39/00 ,  C08G77/60 ,  C08F2/00 ,  C08F2/10 ,  C08F2/42 ,  C10G25/00 ,  C10G35/06 ,  C10G45/00 ,  C10G45/04 ,  C10G45/34 ,  C10G45/44 ,  C10G45/46 ,  C10G45/60 ,  C10G45/64 ,  C10G47/02 ,  C10G50/00 ,  C10M177/00 ,  C10K1/32 ,  C10M101/02 ,  C07F7/00 ,  C08F4/659 ,  C08F4/6592 ,  B01J29/03

Abstract: A catalyst system comprising a combination of: 1) one or more catalyst compounds having at least one nitrogen linkage and at least one oxygen linkage to a transition metal; 2) a support comprising an organosilica material, which is a mesoporous organosilica material; and 3) an optional activator. Useful catalysts include ONNO-type transition metal catalysts, ONYO-Type transition metal catalysts, and/or oxadiazole transition metal catalysts. The organosilica material is a polymer of at least one monomer of Formula [z′OZ2 SiCH2]3(l), where Z1 represents a hydrogen atom, a C1-C4alkyl group, or a bond to a silicon atom of another monomer and Z2 represents a hydroxyl group, a C1-C4alkoxy group, a C1-C6alkyl group, or an oxygen atom bonded to a silicon atom of another monomer. This invention further relates to processes to polymerize olefins comprising contacting one or more olefins with the above catalyst system.