公开(公告)号：US20160237474A1

公开(公告)日：2016-08-18

申请号：US15016713

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey Marks

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2201/04 ,  G01N2201/068 ,  G01N2201/12746

Abstract: The present teachings relate to a method and system for normalizing spectra across multiple instruments. In an embodiment of the present invention, the method comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors for each filter pair to result in corrected pure dye spectra, then normalized and the multicomponenting data can be extracted.

Abstract translation: 本教导涉及用于在多个仪器上归一化光谱的方法和系统。 在本发明的一个实施例中，该方法包括至少一个参考仪器和测试仪器。 每个仪器包括至少一个激励滤波器和成对布置的至少一个发射滤波器。 每个仪器还包括包含多个孔的纯染料板。 每个孔含有多种染料，其中每种染料包含荧光组分。 从每个仪器获得的荧光光谱可以通过多种滤光片组合为每种染料获得，从而有助于参考仪器的纯染料矩阵Mref和测试仪器的纯染料矩阵M. 然后可以将纯染料光谱乘以每个过滤器对的校正因子，以产生校正的纯染料光谱，然后进行归一化，并且可以提取多组分数据。

公开(公告)号：US20150368702A1

公开(公告)日：2015-12-24

申请号：US14750463

申请日：2015-06-25

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Stephen J. Gunstream

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G16B30/00 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (RT-PCR) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same RT-PCR thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample. A set of maxima values and a set of minimum values, and/or other calibration information useful for adjusting emission data for sample dyes can be recorded, for example, for 10 cycles, 20 cycles, or each cycle of a complete RT-PCR run. Such testing of dye response under realistic operating conditions can enable more accurate characterization of plate, dye, filter, or instrument response and therefore more accurate calibration corrections and other and/or adjustments.

Abstract translation: 本教导涉及在实时聚合酶链反应（RT-PCR）或其他扩增反应期间产生校准信息的方法。 样品孔板或其它载体可以含有一种或多种经受相同RT-PCR热循环或用于在生物样品上进行扩增或其他反应的其他条件的染料或其它参考物质。 可以记录一组最大值和一组最小值，和/或可用于调整样品染料的发射数据的其他校准信息，例如10个循环，20个循环或完整RT-PCR运行的每个循环 。 在真实的操作条件下对染料响应的这种测试可以实现板，染料，过滤器或仪器响应的更精确的表征，因此可以进行更精确的校准校正和其他和/或调整。

公开(公告)号：US09167179B2

公开(公告)日：2015-10-20

申请号：US13371414

申请日：2012-02-11

Applicant: Deron L. Linsacum ,  Nils A. Lavik ,  Greg Kwan

Inventor： Deron L. Linsacum ,  Nils A. Lavik ,  Greg Kwan

IPC: G01D18/00 ,  G12B13/00 ,  H04N5/33 ,  H04N5/365 ,  G01J5/52 ,  H04N5/361 ,  G01J5/00 ,  G01J5/20 ,  G01J5/10

CPC classification number: H04N5/33 ,  G01J5/522 ,  G01J2005/0048 ,  G01J2005/0077 ,  G01J2005/106 ,  G01J2005/202 ,  G01N2201/1211 ,  G01N2201/12707 ,  G01N2201/12715 ,  G01N2201/12746 ,  G01N2201/12753 ,  H04N5/361 ,  H04N5/3651 ,  H04N5/3655

Abstract: On-board non-uniformity correction calibration methods for a microbolometer focal plane array in a thermal camera are disclosed. The methods include performing first calculations in the processor unit of the thermal camera to generate and apply a set of coarse correction bias voltages to the detector elements. The method also includes performing calculations in the external computer based on image data collected by the thermal camera with the coarse correction bias voltages applied to the detector elements to generate a set of fine correction bias voltages. The method also includes downloading the fine correction bias voltages to the thermal camera and applying the fine correction voltages to the detector elements to establish a fine calibration of the microbolometer focal plane array.

Abstract translation: 公开了一种用于热照相机中的微测光计焦平面阵列的车载非均匀性校正校准方法。 这些方法包括在热像仪的处理器单元中执行第一次计算，以生成并将一组粗校正偏置电压应用于检测器元件。 该方法还包括基于由热摄像机收集的图像数据在外部计算机中执行计算，其中施加到检测器元件的粗略校正偏置电压以产生一组精细校正偏置电压。 该方法还包括将精细校正偏置电压下载到热摄像机并将精细校正电压施加到检测器元件以建立微测光计焦平面阵列的精细校准。

公开(公告)号：US20150260648A1

公开(公告)日：2015-09-17

申请号：US14213064

申请日：2014-03-14

Applicant: DATACOLOR HOLDING AG

Inventor： Zhiling Xu

IPC: G01N21/47

CPC classification number: G01N21/59 ,  G01N21/4738 ,  G01N21/94 ,  G01N2021/4707 ,  G01N2021/4735 ,  G01N2021/4773 ,  G01N2201/0633 ,  G01N2201/0634 ,  G01N2201/065 ,  G01N2201/12746

Abstract: The present invention is directed to an apparatus and method for measuring the haze value of transmissive samples. The apparatus comprises a first light source selectively configurable to emit a first light beam, a second light source selectively configurable to emit a second light beam, an integrating sphere having an outer surface and an inner surface, the inner surface configured to reflect light incident upon the inner surface, the inner surface further enclosing an interior volume. The integrating sphere is further equipped with an exit port configured to emit light from the interior volume of the integrating sphere. The exit port is positioned such that light from the first light source exits the integrating sphere without obstruction, and light from the second light source is diffused on the interior surface of the integrating sphere prior to exiting the exit port. A light detector is also included and is configured to generate a light-intensity signal when light exiting the integrating sphere has passed through a sample and is incident on the light detector. The apparatus further includes a processor configured to receive the light-intensity signal and generate an output signal to a user indicating the haze value. The present invention is also directed to a method of calculating the haze value of a sample using a stored calibration value, a diffuse transmission value (sample-absent diffuse light-intensity value), a direct transmission value (sample-absent direct light-intensity value), a measured diffuse transmission value (sample-present diffuse light-intensity value) and a measured direct transmission value (sample-present direct light-intensity value).

Abstract translation: 本发明涉及一种用于测量透射样品的雾度值的装置和方法。 该装置包括可选择性地配置为发射第一光束的第一光源，可选择地配置为发射第二光束的第二光源，具有外表面和内表面的积分球，所述内表面被配置为反射入射到其上的光 内表面，内表面还包围内部体积。 积分球还配备有出口端口，其被配置为从积分球的内部空间发射光。 出口端口被定位成使得来自第一光源的光不会阻塞地离开积分球，并且来自第二光源的光在离开出口之前扩散到积分球的内表面上。 还包括光检测器，并且被配置为当离开积分球的光已经通过样品并入射到光检测器上时产生光强度信号。 该装置还包括处理器，其被配置为接收光强度信号并且向用户生成指示雾度值的输出信号。 本发明还涉及一种使用存储的校准值，漫射透射值（无采样扩散光强度值），直接透射值（无采样直接光强度）的样品的雾度值来计算雾度值的方法 值），测量的漫射透射值（样品存在的漫射光强度值）和测量的直接透射值（样品存在的直接光强度值）。

公开(公告)号：US20150160134A1

公开(公告)日：2015-06-11

申请号：US14562060

申请日：2014-12-05

Applicant: Quidel Corporation

Inventor： David Dickson Booker ,  Jhobe Steadman

IPC: G01N21/64 ,  G01N33/543

CPC classification number: G01N21/645 ,  G01N21/274 ,  G01N21/278 ,  G01N33/54386 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/10 ,  G01N2201/12746 ,  G01N2201/12753 ,  G01N2201/12761

Abstract: Disclosed herein is a method for improving the precision of a test result from an instrument with an optical system that detects a signal. The method comprises including in the instrument a normalization target disposed directly or indirectly in the optical path of the optical system. Also disclosed are instruments comprising a normalization target, and systems comprising such an instrument and a test device that receives a sample suspected of containing an analyte.

Abstract translation: 这里公开了一种用于提高具有检测信号的光学系统的仪器的测试结果的精度的方法。 该方法包括在仪器中包括直接或间接地设置在光学系统的光路中的归一化目标。 还公开了包括归一化目标的仪器，以及包含这样的仪器和接收怀疑含有分析物的样品的测试装置的系统。

公开(公告)号：US08742327B2

公开(公告)日：2014-06-03

申请号：US14051132

申请日：2013-10-10

Applicant: Bayer HealthCare LLC

Inventor： Russell J Micinski ,  Andrew J Dosmann ,  Steven C Charlton

IPC: G12B13/00 ,  G01N21/27 ,  A61B5/1495

CPC classification number: A61B5/1495 ,  G01N21/274 ,  G01N21/8483 ,  G01N27/4163 ,  G01N33/48771 ,  G01N2021/8494 ,  G01N2021/8645 ,  G01N2201/12746

Abstract: A method of determining auto-calibrating information of a test sensor includes providing an optical read head that includes a light source, a light guide and a detector. The read head forms an opening that is sized to receive a test sensor. The detector includes a linear-detector array or single detector. A test sensor is provided having apertures formed therein. The test sensor is placed in the opening of the optical read head. Light is transmitted from the light source through the apertures. The light transmitted through the apertures using the detector or detecting the absence of light being transmitted through the test sensor using the detector is detected. The detected light or the absence of detected light information from the detector is used to determine the auto-calibration information of the test sensor.

Abstract translation: 确定测试传感器的自动校准信息的方法包括提供包括光源，光导和检测器的光学读取头。 读取头形成一个开口，其大小用于接收测试传感器。 检测器包括线性检测器阵列或单个检测器。 提供具有形成在其中的孔的测试传感器。 测试传感器被放置在光学读取头的开口中。 光从光源通过孔传输。 检测通过检测器透过孔的光，或检测不使用检测器透过测试传感器的光。 使用来自检测器的检测到的光或不存在检测到的光信息来确定测试传感器的自动校准信息。

公开(公告)号：US20130041595A1

公开(公告)日：2013-02-14

申请号：US13584950

申请日：2012-08-14

Applicant: Stephen J. GUNSTREAM

Inventor： Stephen J. GUNSTREAM

IPC: G06F19/00

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G16B30/00 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。

公开(公告)号：US08285492B2

公开(公告)日：2012-10-09

申请号：US12534742

申请日：2009-08-03

Applicant: Stephen J. Gunstream

Inventor： Stephen J. Gunstream

IPC: G01N21/17 ,  G01N21/01 ,  G01N21/31 ,  G01B9/08

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G06F19/22 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。

公开(公告)号：US20110231132A1

公开(公告)日：2011-09-22

申请号：US13070273

申请日：2011-03-23

Applicant: Stephen J. GUNSTREAM

Inventor： Stephen J. GUNSTREAM

IPC: G06F17/18 ,  G06F19/00

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G06F19/22 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。

公开(公告)号：US20100198525A1

公开(公告)日：2010-08-05

申请号：US12534742

申请日：2009-08-03

Applicant: Stephen J. Gunstream

Inventor： Stephen J. Gunstream

IPC: G01N31/00 ,  G06F19/00

CPC classification number: C12Q1/6851 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2201/12746 ,  G06F19/22 ,  C12Q2545/113 ,  C12Q2545/101

Abstract: Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

Abstract translation: 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。