公开(公告)号：US20170065977A1

公开(公告)日：2017-03-09

申请号：US15183406

申请日：2016-06-15

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Kosar B. Parizi ,  Mark F. Oldham

IPC: B01L3/00 ,  B01L7/00 ,  B03C5/02 ,  B03C5/00 ,  B03C1/30 ,  C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other.

Abstract translation: 本发明的方面包括通过利用未标记的非可合并核苷酸或通过重新构建基于集落的测序反应来提高测序反应的准确性和读取长度的方法。 其他方面包括用于改进与珠粒相关的生物反应测量的系统和装置，其可以通过与所述珠粒相关联的抗衡离子的电流测量方法，由于存在与所述珠粒结合或相关联的反应物，其中用于产生和 测量所述电流可以在所述焊道的德拜长度内。 本发明的其它方面包括用于确定输入样品的浓度的方法，用于重复使用阵列的装置，用于分离具有不同电荷水平的珠子的方法和装置。

公开(公告)号：US09562837B2

公开(公告)日：2017-02-07

申请号：US13855318

申请日：2013-04-02

Applicant: Raindance Technologies, Inc.

Inventor： Darren R. Link

IPC: B01L3/00 ,  G01N1/38 ,  C12Q1/68 ,  B01J19/00 ,  B01F13/00 ,  B03C5/00 ,  B03C5/02 ,  B01F3/08 ,  C12Q1/00 ,  G01N35/08 ,  G01N15/14 ,  C12N15/10 ,  G01N1/10 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00

CPC classification number: C12Q1/6848 ,  B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326 ,  C12Q2535/101 ,  C12Q2563/149

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

Abstract translation: 本发明一般涉及用于从容器移动液滴的组件，其有利于液滴的收集和转移同时最小化样品损失。 在某些方面，组件包括至少一个液滴形成模块，其中模块被配置成形成由不混溶流体包围的液滴。 组件还包括至少一个包括出口的室，其中室被构造成接收液滴和不混溶的流体，并且其中出口构造成仅基本上容纳液滴。 组件还包括通道，其构造成使得液滴形成模块和腔室经由通道彼此流体连通。 在其他方面，组件包括多个中空构件，其中中空构件是通道，构件中构件与容器相互作用。 多个中空构件包括构造成排出与容器中的液滴不混溶的流体的第一构件和构造成基本上仅从容器中滴下的第二构件。 组件还包括主通道，其中第二构件与主通道流体连通。 组件还包括连接到主通道的至少一个分析模块。

公开(公告)号：US20170028408A1

公开(公告)日：2017-02-02

申请号：US15302408

申请日：2015-04-02

Applicant: APOCELL, INC.

Inventor： Anoop MENACHERY ,  Ronald PETHIG

IPC: B03C5/00 ,  G01N33/487 ,  G01N27/447

CPC classification number: B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N27/44713 ,  G01N27/44721 ,  G01N33/48707

Abstract: The present invention provides a new method for accurately identifying DEP cross-over frequencies of one or more particles in a sample, and quickly and efficiently conveying that information to assist in the separation, e.g., DEP separation, or analysis of the one of more particles under examination or investigation. The present invention also provides an apparatus and method for monitoring the dielectrophoretic response of one or more particles and determining the DEP cross-over frequency of particles of interest.

Abstract translation: 本发明提供了一种用于准确地识别样品中一种或多种颗粒的DEP交叉频率的新方法，并且快速有效地传达该信息以帮助分离，例如DEP分离或分析更多的颗粒之一 正在考察或调查。 本发明还提供了一种用于监测一种或多种颗粒的介电电泳响应并确定感兴趣的颗粒的DEP交叉频率的装置和方法。

公开(公告)号：US09546392B2

公开(公告)日：2017-01-17

申请号：US14976895

申请日：2015-12-21

Applicant: BIOCEP LTD.

Inventor： Aharon Lamish ,  Ofer Klein

IPC: G01N30/00 ,  B01J19/08 ,  G01N31/00 ,  C12Q1/24 ,  B03C1/033 ,  B03C1/28 ,  C12M1/00 ,  C12N1/02

CPC classification number: C12N1/02 ,  B03C1/0335 ,  B03C1/288 ,  B03C2201/26 ,  C12M47/02 ,  C12Q1/24

Abstract: A magnetic separation system configured to separate with high qualitative and quantitative yield magnetized cells from cell mixtures, comprising at least one electromagnet structured to generate a magnetic field flux about a plurality of separation zones and sufficient to attract a majority of the magnetized cells in the mixture, and a pump to drive the cell mixture at a controlled flow rate through a tube disposed within the zones thereby separating a majority of the magnetized cells from the mixture. The system is particularly useful to retrieve rare cells from a fluid mixture of cells having low abundance of the rare cells relative to the rest of the cells while sustaining viability of the cells.

Abstract translation: 一种磁分离系统，其被配置为以高定性和定量的方式从细胞混合物中分离产生磁化细胞，其包括至少一个电磁体，其被构造为在多个分离区周围产生磁场通量并且足以吸引混合物中的大部分磁化细胞 以及泵，其以受控的流速通过设置在所述区域内的管驱动细胞混合物，从而将大部分磁化细胞与混合物分离。 该系统特别可用于从维持细胞活力的细胞中相对于细胞的其余部分从具有低丰度稀有细胞的细胞的流体混合物中检索稀有细胞。

公开(公告)号：US20160370279A1

公开(公告)日：2016-12-22

申请号：US15179484

申请日：2016-06-10

Applicant: IMEC VZW

Inventor： Richard Min ,  Liesbet Lagae ,  Chengxun Liu ,  Chengjun Huang

IPC: G01N15/10 ,  B01L3/00

CPC classification number: G01N15/1031 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/06 ,  B01L2300/0864 ,  B01L2300/12 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N15/1056 ,  G01N35/0098 ,  G01N2015/1081 ,  G01N2015/149

Abstract: A microfluidic magnetic selector comprises a microfluidic channel comprising at least one bifurcation, forming a selection portion of the selector and splitting the microfluidic channel into a main channel and at least one selection channel; at least one magnetic flux concentrator for concentrating a magnetic flux at the level of the bifurcation, and means for generating a magnetic field within the magnetic flux concentrator, and a controller for controlling magnetic pulses through the magnetic flux concentrator.

Abstract translation: 微流体磁选择器包括包含至少一个分叉的微流体通道，形成选择器的选择部分并将微流体通道分成主通道和至少一个选择通道; 用于将磁通集中在分叉水平的至少一个磁通集中器，以及用于在磁通量集中器内产生磁场的装置，以及用于控制通过磁通量集中器的磁脉冲的控制器。

公开(公告)号：US09517474B2

公开(公告)日：2016-12-13

申请号：US13873424

申请日：2013-04-30

Applicant: University of Georgia Research Foundation, Inc.

Inventor： Leidong Mao ,  Taotao Zhu ,  Mark A. Eiteman

IPC: B03C1/32 ,  B03C1/28 ,  G01N27/74

CPC classification number: B03C1/288 ,  B03C1/32 ,  B03C2201/18 ,  B03C2201/26 ,  C12N13/00 ,  G01N27/74

Abstract: Embodiments of the present disclosure provide for devices, methods for separating particles, and the like. In general, embodiments of the present disclosure include non-uniform magnetic field-assisted processes and devices for the separation of particles (e.g., cells) within a magnetic fluid. Under non-uniform magnetic fields, particles such as cells can experience the generated magnetic field direction to produce a magnetic buoyancy force, analogous to buoyancy force, as magnitude of the force is proportional to the volume of cell. This force can be used to spatially separate cells of different sizes. In some embodiments, devices for separating particles are provided having a magnetic device configured to direct a non-uniform magnetic force onto the magnetic fluid and the particles; and a plurality of outlets, wherein the non-uniform magnetic force causes the types of particles to be separated and flow into different outlets.

Abstract translation: 本公开的实施例提供了用于分离颗粒的装置，方法等。 通常，本公开的实施例包括用于分离磁性流体内的颗粒（例如，电池）的非均匀磁场辅助方法和装置。 在非均匀磁场下，诸如电池的颗粒可以经历所产生的磁场方向以产生类似于浮力的磁浮力，因为力的大小与电池的体积成比例。 该力可用于空间分离不同大小的细胞。 在一些实施例中，提供了用于分离颗粒的装置，其具有被配置成将非均匀磁力引导到磁性流体和颗粒上的磁性装置; 和多个出口，其中所述非均匀磁力使所述颗粒的类型分离并流入不同的出口。

公开(公告)号：US20160320378A1

公开(公告)日：2016-11-03

申请号：US15210634

申请日：2016-07-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543 ,  C12N15/10 ,  B03C1/30 ,  B03C5/00 ,  B03C1/28 ,  G01N33/561 ,  B01L3/00

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

公开(公告)号：US20160299138A1

公开(公告)日：2016-10-13

申请号：US15094045

申请日：2016-04-08

Applicant: The Curators of the University of Missouri

Inventor： Mahmoud Almasri ,  Shibajyoti Ghosh Dastider ,  Shuping Zhang ,  Majed El Dweik ,  Nuh Sadi Yuksek ,  Ibrahem Jasim

IPC: G01N33/569 ,  G01N27/27 ,  B01L3/00 ,  B03C5/02 ,  G01N33/543 ,  G01N27/447 ,  B03C5/00

CPC classification number: G01N33/56916 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0424 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N27/06 ,  G01N33/5438 ,  G01N2333/245 ,  G01N2333/255

Abstract: Disclosed herein are example embodiments of a transformative sensor apparatus that is capable of detecting and quantifying the presence of a substance of interest such as a specified bacteria within a sample via changes in impedance exhibited by a detection electrode array. In an example embodiment, sensitivity is improved by including a focusing electrode array in a rampdown channel to focus a concentration of the substance of interest into a detection region. The focusing electrodes include an opposing pair of electrodes in a rampdown orientation. The focusing electrode may also include tilted thin film finger electrodes extending from the rampdown electrodes. In another example embodiment, trapping electrodes are positioned to trap a concentration of the substance of interest onto the detection electrode array.

Abstract translation: 本文公开了一种变换传感器装置的示例性实施例，其能够通过检测电极阵列所呈现的阻抗变化来检测和定量样品中特定细菌的存在。 在示例性实施例中，通过将聚焦电极阵列包括在下降通道中来将感兴趣物质的浓度聚焦到检测区域来提高灵敏度。 聚焦电极包括处于倾斜方向的相对电极对。 聚焦电极还可以包括从斜坡电极延伸的倾斜的薄膜指状电极。 在另一示例性实施例中，定位俘获电极以将感兴趣物质的浓度捕获到检测电极阵列上。

公开(公告)号：US20160266026A1

公开(公告)日：2016-09-15

申请号：US15163890

申请日：2016-05-25

Applicant: Yale University

Inventor： Hur Koser

IPC: G01N15/10 ,  C12M1/00 ,  B01L3/00 ,  C12N13/00

CPC classification number: G01N15/1056 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/06 ,  B01L2300/0848 ,  B03C1/023 ,  B03C1/23 ,  B03C1/253 ,  B03C1/288 ,  B03C1/32 ,  B03C2201/18 ,  B03C2201/24 ,  B03C2201/26 ,  C12M47/04 ,  C12N13/00 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081

Abstract: A device for separating a sample of cells suspended in a bio-compatible ferrofluid is described, The device includes a microfluidic channel having a sample inlet, at least one outlet and a length between the same inlet and the at least one outlet, wherein a sample can be added to the sample inlet and flow along the microfluidic channel length to the at least one outlet. The device includes a plurality of electrodes and a power source for applying a current to the plurality of electrodes to create a magnetic field pattern along the microfluidic channel length. The present invention also includes a method of using said device for separating at least one cell type.

公开(公告)号：US20160250637A1

公开(公告)日：2016-09-01

申请号：US15031308

申请日：2014-10-27

Applicant: MONASH UNIVERSITY

Inventor： Adrian NEILD ,  Tuncay ALAN ,  David John COLLINS

IPC: B01L3/00 ,  G01N27/447 ,  G01N15/10

CPC classification number: B01L3/502715 ,  B01D21/283 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/06 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0436 ,  B01L2400/0496 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/0255 ,  G01N15/1031 ,  G01N15/1056 ,  G01N27/4473 ,  G01N27/44791 ,  G01N2001/4038 ,  G01N2015/0288 ,  G01N2015/1081

Abstract: A microfluidic device for separating or sorting particles in a fluid including: a substrate; a plurality of interdigital transducers on the substrate; a microfluidic channel adapted to have fluid flow within, located over the interdigital transducers, the microfluidic channel having a width, wherein: the interdigital transducers are located within the width of the microfluidic channel; and application of a signal to the interdigital transducers produces a force field at an angle to the fluid flow direction within the microfluidic channel. In addition, a method for separating or sorting particles using a device having a plurality of interdigital transducers on a substrate and a microfluidic channel located over the interdigital transducers, the method including: positioning the interdigital transducers within the microfluidic channel width; inserting into the microfluidic channel a solution having particles with various properties; and applying a signal to the interdigital transducers to produce a force field at an angle to a fluid flow direction within the microfluidic channel to sort and/or physically separate the particles into groups of particles with the same property.

Abstract translation: 一种用于分离或分选流体中的颗粒的微流体装置，包括：基底; 在所述基板上的多个叉指换能器; 微流体通道，其适于在所述叉指式换能器之间具有流体流动，所述微流体通道具有宽度，其中：所述叉指换能器位于所述微流体通道的宽度内; 并且将信号施加到叉指换能器产生与微流体通道内的流体流动方向成一定角度的力场。 另外，使用在基板上具有多个叉指换能器的装置和位于叉指换能器之上的微流体通道的分离或分选颗粒的方法，所述方法包括：将所述叉指式换能器定位在所述微流体通道宽度内; 将具有各种性质的颗粒的溶液插入到微流体通道中; 以及将信号施加到叉指换能器以产生与微流体通道内的流体流动方向成一定角度的力场，以将颗粒分类和/或物理地分离成具有相同性质的颗粒组。