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    웨스트나일바이러스 유지 및 검출용 배지와 이를 이용한 웨스트나일바이러스 검출 또는 역가검정법
    2.
    发明公开
    웨스트나일바이러스 유지 및 검출용 배지와 이를 이용한 웨스트나일바이러스 검출 또는 역가검정법 无效
    用于检测和维护西尼病毒的介质和使用其的西兰病毒的检测和测序方法

    公开(公告)号：KR1020120086024A

    公开(公告)日：2012-08-02

    申请号：KR1020110007213

    申请日：2011-01-25

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 예정용 ,  박지용 ,  이지혜 ,  박희수 ,  문진산 ,  조인수

    IPC: C12Q1/70 ,  C12Q1/04 ,  C12N7/00

    CPC classification number: C12Q1/045 ,  C12N7/00 ,  G01N31/221 ,  G01N33/52

    Abstract: PURPOSE: A medium for maintainig and determining west nile is provided to reduce the exposure by high risk virus and to shorten the use of harmful reagents. CONSTITUTION: A medium for maintaining and detecting west nile contains alpha-MEM or DMEM containing 1-10 g/L of glucose and pH indicator. The pH indicator is phenol red. A potency assay method for detecting west nile comprises: a step of culturing west nile host cells and a sample in the medium; and a step of measuring the pH concentration of the medium to detecting west nile virus and tittering west nile in a sample. The host cells are monkey kidney cells.

    Abstract translation: 目的：提供维护和确定西尼罗河的介质，以减少高风险病毒的暴露并缩短有害试剂的使用。 构成：用于维持和检测西尼罗河的培养基含有含有1-10克/升葡萄糖和pH指示剂的α-MEM或DMEM。 pH指示剂为酚红。 用于检测西尼罗河的效力测定方法包括：培养西尼罗河宿主细胞和培养基中的样品的步骤; 以及测定介质中检测西尼罗病毒并滴定样品中西尼罗河的pH浓度的步骤。 宿主细胞是猴肾细胞。

    구제역 바이러스 억제를 위한 ｓｉＲＮＡ를 발현하는 재조합 아데노바이러스
    3.
    发明公开
    구제역 바이러스 억제를 위한 ｓｉＲＮＡ를 발현하는 재조합 아데노바이러스 有权
    重组腺病毒表达小干扰RNA以抑制病毒性和病毒性病毒

    公开(公告)号：KR1020100059035A

    公开(公告)日：2010-06-04

    申请号：KR1020080117663

    申请日：2008-11-25

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 김수미 ,  박종현 ,  이광녕 ,  고영준 ,  박지용 ,  주이석

    IPC: C12N15/86 ,  C12N15/40 ,  C12N15/10 ,  C12N15/09

    Abstract: PURPOSE: A recombinant adenovirus expressing siRNA for suppressing and preventing foot-and-mouth virus is provided to generate siRNA targeting foot-and-mouth virus 2B and 3C genes and to ensure antiviral effect. CONSTITUTION: A recombinant adenovirus comprises a nucleotide of sequence number 3 or 5 and complementary nucleotide thereof(sequence number 4 or 6). An siRNA which specifically binds to the foot-and-mouth virus is synthesized from a DNA having a nucleotide of sequence number 1 or 2, or complementary nucleotide thereof. An antiviral formulation for foot-and-mouth contains the recombinant adenovirus expressing siRNA as an active ingredient.

    Abstract translation: 目的：提供用于抑制和预防口蹄疫病毒的表达siRNA的重组腺病毒，以产生针对口蹄疫病毒2B和3C基因的siRNA，并确保抗病毒效果。 构成：重组腺病毒包含序列号3或5及其互补核苷酸（序列号4或6）的核苷酸。 特异性结合口蹄疫病毒的siRNA由序列号1或2的核苷酸或其互补核苷酸的DNA合成。 用于口蹄疫的抗病毒制剂含有表达siRNA作为活性成分的重组腺病毒。

    곤충세포 발현 재조합 3ＡＢＣ 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법
    9.
    发明公开
    곤충세포 발현 재조합 3ＡＢＣ 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법 有权
    使用在INSEC细胞和单克隆抗体中表达的重组蛋白3ABC非结构蛋白的足球疾病的诊断方法

    公开(公告)号：KR1020030052858A

    公开(公告)日：2003-06-27

    申请号：KR1020010082976

    申请日：2001-12-21

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 권창희 ,  현방훈 ,  고영준 ,  이광녕 ,  구복경 ,  엄재구 ,  나진주 ,  남향미 ,  박지용 ,  계수정 ,  김인중 ,  이세영 ,  최광석 ,  손현주 ,  주이석

    IPC: C12N15/33

    CPC classification number: C12N15/866 ,  C07K14/005 ,  C07K16/1009 ,  C07K2317/14 ,  C12N5/163 ,  C12N2770/32131 ,  G01N33/56983 ,  G01N33/577 ,  G01N2333/09

    Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.

    Abstract translation: 目的：提供使用在昆虫细胞中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速，准确地诊断口蹄疫。 构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组体3ABC非结构蛋白的基​​因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒通过 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。重组体3ABC非结构蛋白用重组杆状病毒感染昆虫细胞表达。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白质导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 口蹄疫的诊断方法包括以下步骤：（1）在包被缓冲溶液中稀释重组体3ABC非结构蛋白，将稀释液倒入平板上; （2）使测试血清与板反应; （3）使与测试血清中的口蹄疫病毒抗体结合并具有酶，放射性物质或荧光材料的缀合物与测试血清反应; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。

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