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    곤충세포 발현 재조합 3ＡＢＣ 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법
    1.
    发明授权
    곤충세포 발현 재조합 3ＡＢＣ 비구조단백질 항원 및 단클론항체를 이용한 구제역 진단방법 有权
    AB세포발현재조합3ABC비구조단백질항원및단클론항체를이용한구제역진단방곤충

    公开(公告)号：KR100430934B1

    公开(公告)日：2004-05-14

    申请号：KR1020010082976

    申请日：2001-12-21

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 권창희 ,  현방훈 ,  고영준 ,  이광녕 ,  구복경 ,  엄재구 ,  나진주 ,  남향미 ,  박지용 ,  계수정 ,  김인중 ,  이세영 ,  최광석 ,  손현주 ,  주이석

    IPC: C12N15/33

    Abstract: PURPOSE: Diagnostic methods of foot-and-mouth disease using recombinant 3ABC non-structural protein expressed in insect cells and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. A recombinant baculovirus to be expressed in insect cells is prepared by co-transfection with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed by infection of the insect cells with the recombinant baculovirus. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the diluate on a plate; (2) reacting the testing serum with the plate; (3) reacting a conjugate, which binds with an antibody for foot-and-mouth disease virus in the testing serum and has an enzyme, a radioactive material or a fluorescent material, with the testing serum; and (4) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.

    Abstract translation: 目的：提供使用在昆虫细胞和单克隆抗体中表达的重组3ABC非结构蛋白的口蹄疫诊断方法，从而比现有方法更快速和准确地诊断口蹄疫。 构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基​​因具有SEQ ID NO：1的核苷酸序列。在昆虫细胞中表达的重组杆状病毒由 用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体共转染。通过用重组杆状病毒感染昆虫细胞表达重组3ABC非结构蛋白。 通过将在大肠杆菌中表达的重组3ABC非结构蛋白导入动物中，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合来制备杂交瘤细胞系3F-11（KCTC 10138BP）。 从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。 一种口蹄疫诊断方法，包括以下步骤：（1）将重组3ABC非结构蛋白稀释于包被缓冲液中，并将稀释液倒入平板中; （2）使测试血清与平板反应; （3）使与试验血清中的口蹄疫病毒抗体结合的缀合物与测试血清具有酶，放射性物质或荧光物质; 和（4）测量酶反应的强度，荧光反应或与缀合物的辐射反应。

    흑염소의 프리온 단백질을 코딩하는 유전자를 포함하는 발현 벡터 및 이에 의하여 형질도입된 세포
    5.
    发明公开
    흑염소의 프리온 단백질을 코딩하는 유전자를 포함하는 발현 벡터 및 이에 의하여 형질도입된 세포 有权
    表达载体包含来自CAPRA AEGAGRUS HIRCUS和转移细胞的基因编码蛋白质

    公开(公告)号：KR1020140081447A

    公开(公告)日：2014-07-01

    申请号：KR1020120151209

    申请日：2012-12-21

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 손현주 ,  이윤희 ,  김민정 ,  김효진 ,  박후창 ,  윤은임 ,  조인수

    IPC: C12N15/63 ,  C12N15/12 ,  C12N5/10 ,  C07K14/47

    Abstract: The present invention relates to an expression vector comprising a gene encoding a prion protein of Capra hircus and a cell transduced by the vector. The present invention can be used for manufacturing a cell having polymorphism, which represents Capra hircus bred in Korea, and a transduced mouse; and can be effectively used for developing an animal experimental model for studies including diagnosis of prion diseases which include scrapie occurred in goat family animals, and for confirming if domestic TSE diseases interspecifically spread or not.

    Abstract translation: 本发明涉及一种表达载体，其包含编码Capra hircus的朊病毒蛋白的基因和由载体转导的细胞。 本发明可用于制造具有多形性的细胞，其代表在韩国繁殖的Capra hircus和转导的小鼠; 可以有效地用于研究包括诊断朊病毒疾病的动物实验模型，包括山羊家族动物发生的瘙痒病，以及确认国内TSE疾病是否特异性传播。

    복분자 추출물을 포함하는 소해면상뇌증의 예방 또는 치료용 조성물
    6.
    发明公开
    복분자 추출물을 포함하는 소해면상뇌증의 예방 또는 치료용 조성물 无效
    包含RUBI FRUCTUS提取物的组合物，用于预防或治疗BOVINE SPONIFORM ENCEPHALOPATHY

    公开(公告)号：KR1020120075678A

    公开(公告)日：2012-07-09

    申请号：KR1020100137439

    申请日：2010-12-29

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

    Inventor： 탁동섭 ,  김효진 ,  김민정 ,  손현주 ,  이윤희 ,  윤은임 ,  이원용 ,  조인수

    IPC: A61K36/73 ,  A61P25/28 ,  A61P25/00

    CPC classification number: A61K36/73 ,  A23V2200/322 ,  A61K2236/331 ,  A61K2236/333

    Abstract: PURPOSE: A composition containing Rubi Fructus extract is provided to inhibit modified prion production and to prevent or treat BSE. CONSTITUTION: A veterinary composition for preventing or treating BSE contains Rubi Fructus extract. The extract is prepared using water, low alcohol of C1-C4, and a mixture solvent thereof. The concentration of the extract is 6.25ug/ml. The daily dose of the composition is 0.0001-100 mg/kg. A feed additive composition for preventing or treating BSE contains the extract. A feed contains the extract.

    Abstract translation: 目的：提供含有Rubi Fructus提取物的组合物，以抑制修饰的朊病毒生成并预防或治疗BSE。 组成：用于预防或治疗疯牛病的兽用组合物含有Rubi Fructus提取物。 提取物是使用水，C1-C4的低级醇及其混合溶剂制备的。 提取物的浓度为6.25ug / ml。 组合物的日剂量为0.0001-100mg / kg。 用于预防或治疗BSE的饲料添加剂组合物含有提取物。 Feed包含提取物。

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