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公开(公告)号：AU2009251883A1
公开(公告)日：2009-12-03
申请号：AU2009251883
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： BJORNSON KEITH , EMIG ROBIN , PELUSO PAUL , HE MOLLY , CLARK SONYA , BIBILLO AREK , LEE HAROLD , JIA LEI , CHRISTIANS FRED , PARK INSIL
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerase. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
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公开(公告)号：AU2006330947B2
公开(公告)日：2012-04-12
申请号：AU2006330947
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： BIBILLO AREK , MITSIS PAUL , PHAM THANG , CHRISTIANS FRED C , MURPHY DEVON , PARK INSIL , HANZEL DAVID K , LYLE JOHN , RANK DAVID R , CLARK SONYA , PELUSO PAUL , OTTO GEOFF
IPC: C12Q1/68
Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
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公开(公告)号：CA2720046A1
公开(公告)日：2009-12-03
申请号：CA2720046
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
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公开(公告)号：AU2006330947A1
公开(公告)日：2007-07-05
申请号：AU2006330947
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： BIBILLO AREK , MITSIS PAUL , PHAM THANG , CHRISTIANS FRED C , MURPHY DEVON , PARK INSIL , HANZEL DAVID K , LYLE JOHN , RANK DAVID R , CLARK SONYA , PELUSO PAUL , OTTO GEOFF
IPC: C12Q1/68
Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
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公开(公告)号：WO2007076057A3
公开(公告)日：2008-06-26
申请号：PCT/US2006049122
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA , HANZEL DAVID K , OTTO GEOFF , MURPHY DEVON , PELUSO PAUL , PHAM THANG , RANK DAVID R , MITSIS PAUL , CHRISTIANS FRED C , BIBILLO AREK , PARK INSIL , CLARK SONYA , LYLE JOHN
Inventor： HANZEL DAVID K , OTTO GEOFF , MURPHY DEVON , PELUSO PAUL , PHAM THANG , RANK DAVID R , MITSIS PAUL , CHRISTIANS FRED C , BIBILLO AREK , PARK INSIL , CLARK SONYA , LYLE JOHN
IPC: C12Q1/68
CPC classification number: C12N9/1252 , C07K2319/20 , C12P19/34 , C12Q1/6827 , C12Q1/6869 , C12Y207/07007 , Y02P20/52
Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
Abstract translation: 提供了包含具有改善核苷酸类似物进入活性位点区域并与活性位点区域中的核苷酸类似物配位的特征的聚合酶的组合物。 还提供了制备聚合酶和在测序和DNA复制和扩增中使用聚合酶的方法以及聚合酶活性的动力学模型和使用该模型的计算机实现的方法。
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Patent Agency Ranking6.发明专利
公开(公告)号：ES2614078T3
公开(公告)日：2017-05-29
申请号：ES15168606
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Abstract: Una composición que comprende una ADN polimerasa recombinante, ADN polimerasa recombinante que comprende una secuencia de aminoácidos que es al menos el 80 % idéntica a la SEQ ID NO: 1, ADN polimerasa recombinante que comprende un resto de ácido glutámico, un resto de ácido aspártico, un resto de histidina, un resto de lisina, un resto de arginina o un resto de tirosina en la posición A484, en la que la identificación de las posiciones es con respecto a la SEQ ID NO: 1, y ADN polimerasa recombinante que muestra actividad polimerasa.
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公开(公告)号：AU2009251883B2
公开(公告)日：2014-09-11
申请号：AU2009251883
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： JIA LEI , BJORNSON KEITH , PARK INSIL , CLARK SONYA , CHRISTIANS FRED , EMIG ROBIN , HE MOLLY , PELUSO PAUL , LEE HAROLD , BIBILLO AREK
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
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公开(公告)号：CA2720046C
公开(公告)日：2018-07-24
申请号：CA2720046
申请日：2009-03-30
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： CLARK SONYA , BIBILLO AREK , PELUSO PAUL , CHRISTIANS FRED , HE MOLLY , PARK INSIL , LEE HAROLD , BJORNSON KEITH , JIA LEI , EMIG ROBIN
Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
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公开(公告)号：ES2648313T3
公开(公告)日：2017-12-29
申请号：ES16157104
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC
Inventor： HANZEL DAVID , OTTO GEOFF , MURPHY DEVON , PELUSO PAUL , PHAM THANG , RANK DAVID , MITSIS PAUL , CHRISTIANS FRED , BIBILLO AREK , PARK INSIL , CLARK SONYA , LYLE JOHN
IPC: C12Q1/68
Abstract: Una composición que comprende: una ADN polimerasa recombinante, comprendiendo dicha polimerasa recombinante una secuencia de aminoácidos que es al menos un 70 % idéntica a la SEQ ID NO:1 y comprende una sustitución de aminoácido en un sitio correspondiente a K512 respecto a la SEQ ID NO:1; y un análogo nucleotídico que está marcado en un grupo fosfato, en el que el resto de base del análogo se selecciona del grupo que consiste en adenina, timina, guanina y citidina.
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公开(公告)号：CA2633524A1
公开(公告)日：2007-07-05
申请号：CA2633524
申请日：2006-12-21
Applicant: PACIFIC BIOSCIENCES CALIFORNIA
Inventor： BIBILLO AREK , CHRISTIANS FRED C , PARK INSIL , LYLE JOHN , OTTO GEOFF , PHAM THANG , RANK DAVID R , MURPHY DEVON , CLARK SONYA , MITSIS PAUL , PELUSO PAUL , HANZEL DAVID K
IPC: C12Q1/68
Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
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