公开(公告)号：WO2014149778A1

公开(公告)日：2014-09-25

申请号：PCT/US2014/020887

申请日：2014-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： FIFE, Keith ,  OWENS, Jordan ,  LI, Shifeng ,  BUSTILLO, James

IPC: G01N27/414

CPC classification number: G01N27/414 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N27/4145 ,  G01N27/4148

Abstract: In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A material defines an opening extending to the upper surface of the floating gate conductor. The material comprises a first dielectric underlying a second dielectric. A conductive element contacts the upper surface of the floating gate conductor and extends a distance along a sidewall of the opening, the distance defined by a thickness of the first dielectric.

Abstract translation: 在一个实施例中，描述了化学传感器。 化学传感器包括具有上表面的浮栅导体的化学敏感场效应晶体管。 材料限定了延伸到浮动栅极导体的上表面的开口。 该材料包括位于第二电介质下面的第一电介质。 导电元件接触浮栅导体的上表面，并沿开口的侧壁延伸一段距离，该距离由第一电介质的厚度限定。

公开(公告)号：WO2014130970A2

公开(公告)日：2014-08-28

申请号：PCT/US2014/018110

申请日：2014-02-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CHEN, Mingsong ,  AGUANNO, Mauro ,  BOO, Kuan Moon ,  SOH, Woon Liang Terence ,  FREUDENTHAL, Jacob ,  MARKS, Jeffrey

IPC: G01N21/27

CPC classification number: G01N21/6486 ,  G01N21/0332 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2201/061 ,  G01N2201/0624 ,  G01N2201/0627 ,  G01N2201/121

Abstract: An instrument for processing and/or measuring a biological process contains a sample processing system, an excitation source, an excitation optical system, an optical sensor, and an emission optical system. The sample processing system is configured to retain a first sample holder and a second sample holder, wherein the number of sample cells is different for each sample holder or a characteristic dimension for the first sample cells is different from that of the second sample holder. The instrument also includes an excitation source temperature controller comprising a temperature sensor that is coupled to the excitation source. The temperature controller is configured to produce a first target temperature when the first sample holder is retained by the instrument and to produce a second target temperature when the second sample holder is retained by the instrument.

Abstract translation: 用于处理和/或测量生物过程的仪器包括样品处理系统，激发源，激发光学系统，光学传感器和发射光学系统。 样品处理系统被配置为保持第一样品保持器和第二样品保持器，其中样品池的数量对于每个样品保持器是不同的，或者第一样品池的特征尺寸与第二样品保持器的特征尺寸不同。 仪器还包括激发源温度控制器，其包括耦合到激发源的温度传感器。 温度控制器被配置为当第一样品保持器被仪器保持时产生第一目标温度，并且当第二样品保持器被仪器保持时产生第二目标温度。

公开(公告)号：WO2014110172A1

公开(公告)日：2014-07-17

申请号：PCT/US2014/010735

申请日：2014-01-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LEONG, Harrison

IPC: G06F19/18 ,  G06F19/28

CPC classification number: G06F19/18 ,  G01N33/49 ,  G06F19/28

Abstract: In one exemplary embodiment, a computer-implemented method for determining a genetic result from a biological sample is provided. The method includes receiving nucleic acid amplification data of a biological sample, by a processor, from a biological instrument. The method further includes storing translation data, in a memory. The translation data includes a pattern of assay values associated with possible genetic results. The method further includes comparing the translation data with the nucleic acid amplification data, by the processor, to generate the genetic result of the biological sample. Moreover, the method includes displaying the genetic result, on a display, to a user.

Abstract translation: 在一个示例性实施例中，提供了用于确定生物样品的遗传结果的计算机实现的方法。 该方法包括通过处理器从生物仪器接收生物样品的核酸扩增数据。 该方法还包括将翻译数据存储在存储器中。 翻译数据包括与可能的遗传结果相关联的测定值的模式。 该方法还包括通过处理器比较翻译数据与核酸扩增数据，以产生生物样品的遗传结果。 此外，该方法包括将显示器上的遗传结果显示给用户。

公开(公告)号：WO2014043581A1

公开(公告)日：2014-03-20

申请号：PCT/US2013/059815

申请日：2013-09-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MAJUMDAR, Nivedita Sumi ,  WESSEL, Thomas ,  WOO, David C. ,  SIKORA, Marcin ,  JANAWAY, Gordon ,  RAIZADA, Shweta ,  LANKESTER, Joanna ,  MARKS, Jeffrey ,  WESSEL, Daniel

IPC: G06F19/24

CPC classification number: G06F19/20 ,  C12Q1/686 ,  G06F19/24

Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

Abstract translation: 提供了一种用于设计数字PCR（dPCR）实验的计算机实现方法。 该方法包括从用户接收优化类型的选择。 优化类型可以使动态范围最大化，例如最小化包括实验所需的反应位点，确定稀释因子或确定检测下限的底物数量。 该方法还包括从用户接收实验的精确度量，以及用于实验的反应部位中靶的最小浓度。 该方法还包括基于优化类型确定实验的一组dPCR实验设计因子。 然后将该组dPCR实验设计因子显示给用户。

公开(公告)号：WO2014039092A1

公开(公告)日：2014-03-13

申请号：PCT/US2013/031562

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  BORMANN CHUNG, Christina ,  MULERO, Julio ,  HENNESSY, Lori

Inventor： BORMANN CHUNG, Christina ,  MULERO, Julio ,  HENNESSY, Lori

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6879 ,  C12Q1/6888 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2525/101 ,  C12Q2525/204 ,  C12Q2537/143

Abstract: Novel Y-STR multiplex analysis designs, primer design, allelic ladders, methods of use and kits are disclosed, including the use of primer sets designed to provide amplicons for at least 11 Y-STR loci having a base pair size of less than about 220 bp, as well as the use of primer sets designed to provide amplicons for at least 22 Y-STR loci including at least 5 rapidly mutating loci.

Abstract translation: 公开了新颖的Y-STR多重分析设计，引物设计，等位基因梯度，使用方法和试剂盒，包括使用设计用于提供具有小于约220的碱基对大小的至少11个Y-STR基因座的扩增子的引物组 bp，以及设计用于为包含至少5个快速突变基因座的至少22个Y-STR基因座提供扩增子的引物组的使用。

公开(公告)号：WO2014003997A1

公开(公告)日：2014-01-03

申请号：PCT/US2013/044180

申请日：2013-06-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： TEBBS, Robert ,  CUMMINGS, Craig ,  BRZOSKA, Pius ,  MATHENY, Sharon ,  O'CONNELL, Catherine ,  FURTADO, Manohar ,  SERGEEV, Nikolay ,  BREVNOV, Maxim

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: The present disclosure describes compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as shiga toxin stx1 and stx2 and eae. Some embodiments relate to detection of STEC microorganisms including an E. coli O26, an E. coli O45, an E. coli O103, an E. coli O111, an E. coli O121 or an E. coli O145. In some embodiments, compositions, methods and kits can detect and identify individual serotypes of shiga toxin producing microorganisms. Workflows for multiple microbe detection and identification are also described.

Abstract translation: 本公开描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子的一种或多种微生物，例如志贺毒素stx1和stx2和eae。 一些实施方案涉及检测STEC微生物，包括大肠杆菌O26，大肠杆菌O45，大肠杆菌O103，大肠杆菌O111，大肠杆菌O121或大肠杆菌O145。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体血清型。 还描述了用于多个微生物检测和鉴定的工作流程。

公开(公告)号：WO2013188839A1

公开(公告)日：2013-12-19

申请号：PCT/US2013/046010

申请日：2013-06-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BORNARTH, Carole ,  LAU, Michael ,  STEVENS, Junko

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2547/107 ,  C12Q2549/101

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

Abstract translation: 本公开涉及用于扩增靶核酸的组合物，方法和试剂盒，同时使用包含至少两种不同热启动机制的双重热启动反应混合物减少非特异性扩增和不期望的扩增产物。

公开(公告)号：WO2013166339A1

公开(公告)日：2013-11-07

申请号：PCT/US2013/039351

申请日：2013-05-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： VASU, Sanjay, K. ,  CHIOU, Henry, C. ,  ROGERS, Jeffrey ,  CISNEROS, Maria ,  LI, Jingqiu ,  LIU, Chao, Yan ,  JONES, Meredith

IPC: C12N5/00

CPC classification number: C07K14/505 ,  C12N5/005 ,  C12N2500/30 ,  C12N2501/065 ,  C12N2501/999 ,  C12N2510/02 ,  C12N2511/00

Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

Abstract translation: 本发明一般涉及可用于将大分子和化合物（例如，核酸分子）引入细胞（例如真核细胞）中的细胞培养基（特别是无血清，非动物衍生的和/或化学上限定的培养基）。 根据本发明，这种引入可以在所述介质的存在下进行。 然后可以在培养基中培养含有这种引入的物质的细胞，并且可以测量或测定引入的细胞对细胞的影响。 特别地，本发明允许将核酸分子（例如，载体）引入细胞（特别是真核细胞）中，以及由细胞中核酸分子编码的蛋白质的表达。 本发明避免了每当与细胞（例如培养细胞与转染细胞）进行不同程序时改变细胞培养基的需要。 本发明还涉及可用于培养和转化/转染细胞的组合物和试剂盒。

公开(公告)号：WO2013165588A1

公开(公告)日：2013-11-07

申请号：PCT/US2013/031515

申请日：2013-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MERRIMAN, Barry ,  MOLA, Paul ,  GARDNER, Mark

IPC: C12Q1/68

CPC classification number: C12Q1/6874

Abstract: A method of genotyping includes applying a sample solution including a plurality of copies of a sample polynucleotide to an array of sensors. The sample polynucleotide includes a region associated with an allele. The method further includes measuring using a plurality of sensors of the array of sensors a characteristic of the region of the plurality of copies of the sample polynucleotide and determining using a computational circuitry and the measured characteristics a statistical value indicative of the allele.

Abstract translation: 基因分型的方法包括将包含多个样品多核苷酸拷贝的样品溶液应用于传感器阵列。 样品多核苷酸包括与等位基因相关的区域。 该方法还包括使用传感器阵列的多个传感器测量样品多核苷酸的多个拷贝区域的特征，并使用计算电路和所测量的特征来确定指示等位基因的统计值。

公开(公告)号：WO2013158982A1

公开(公告)日：2013-10-24

申请号：PCT/US2013/037352

申请日：2013-04-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CHEN, Caifu ,  McFARLAND, Casey, R. ,  KEYS, Jr., David, N.

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2527/119 ,  C12Q2563/159 ,  C12Q2565/607 ,  C12Q2565/301

Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

Abstract translation: 提供了检测靶核酸的方法。 该方法包括将样品分馏成多个样品体积，其中超过50％的级分每个样品体积含有不超过1个靶核酸分子，并且对多个样品体积进行扩增条件。 该方法还包括检测样品体积中的离子浓度的变化，其中存在靶核酸，用扩增的靶核酸计数分数，并测定样品中靶核酸的量。