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    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardware therefor
    111.
    发明公开
    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardware therefor 审中-公开
    使用多个宽带滤光器和相关联的硬件，根据它们的光谱为像素分类成组的方法

    公开(公告)号：EP1207376A1

    公开(公告)日：2002-05-22

    申请号：EP02001413.0

    申请日：1998-08-24

    Applicant: Applied Spectral Imaging Ltd.

    Inventor： Katzir, Nir ,  Garini, Yuval ,  Wine, David ,  Cabib, Dario

    IPC: G01J3/28

    CPC classification number: G01N21/6428 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2021/6441 ,  G02B21/06

    Abstract: A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters (114) and wide-band emission filters (116); (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity (122) as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (d) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.

    Abstract translation: 像素分类成雅丁它们与单一荧光团或从荧光团的多个所选择的荧光团的组合关联的像素组的方法，每一个具有表征激发和发射光谱，并指定激发和发射峰的荧光团，该方法包括 （a）提供对宽带激发滤波器（114）和宽谱带发射滤波器（116）的多个的步骤; （B）每个具有通过宽带激发滤波器之一过滤的光的像素的令人兴奋的荧光团，并穿过其配对的发射滤光器之后作为检索记录发射光强度（122）; （C）重复对所有对过滤器的多个步骤（b），搜索并每个像素是通过尺寸的复数，尺寸等于对的多个数目的数目的向量表示的 过滤器; （D）使用算法的评估每个荧光团在每个像素的所述多个的存在，从而每一个像素分类成一组像素gemäß到其用荧光团的单荧光团或组合的关联。

    Methods and apparati for spectral imaging using interferometers of the low-finesse translating Fabry-Perot type
    115.
    发明公开
    Methods and apparati for spectral imaging using interferometers of the low-finesse translating Fabry-Perot type 失效
    Verfahren und Vorrichtungen zur spektralen Abbildung mittels Fabry-Perot-Interferometern

    公开(公告)号：EP0957345A2

    公开(公告)日：1999-11-17

    申请号：EP99111903.3

    申请日：1993-07-22

    Applicant: Applied Spectral Imaging Ltd.

    Inventor： Cabib, Dario ,  Friedman, Zvi ,  Lipson, Stephen G. ,  Buckwald Robert A.

    IPC: G01J3/28

    CPC classification number: G01J3/2823 ,  G01J3/26 ,  G01J2003/2866

    Abstract: A method and an apparatus of analyzing an optical image of a scene to determine the spectral intensity of each pixel thereof, comprising: collecting incident light from the scene; passing the light through an interferometer which outputs modulated light corresponding to a predetermined set of linear combinations of the spectral intensity of the light emitted from each pixel; focusing the light outputted from the interferometer on a two dimensional detector array; and processing the output of the detector array to determine the spectral intensity of each pixel thereof; the interferometer being of the low-finesse translating Fabry-Perot type in which the optical path difference is varied to modulate the light by translating an element of the interferometer, such that at each instant each detector sees a different point of the scene and its signal is a linear combination of the spectral content of the light coming from each pixel, and that when the scanner completes one interferometer scan, the scene will have been scanned at all relevant linear combinations of the spectral content.

    Abstract translation: 一种分析场景的光学图像以确定其每个像素的光谱强度的方法和装置，包括：从场景收集入射光; 使光通过干涉仪，所述干涉仪输出对应于从每个像素发射的光的光谱强度的预定的一组线性组合的调制光; 将从干涉仪输出的光聚焦在二维检测器阵列上; 并且处理检测器阵列的输出以确定其每个像素的光谱强度; 该干涉仪具有通过转换干涉仪的元件来改变光程差以调制光的低精度翻译法布里 - 珀罗型，使得在每一时刻每个检测器看到场景的不同点及其信号 是来自每个像素的光的光谱含量的线性组合，以及当扫描仪完成一个干涉仪扫描时的光谱内容的线性组合，将以频谱内容的所有相关线性组合扫描场景。

    METHOD FOR CHROMOSOMES CLASSIFICATION
    116.
    发明公开
    METHOD FOR CHROMOSOMES CLASSIFICATION 失效
    分类程序染色体

    公开(公告)号：EP0920537A1

    公开(公告)日：1999-06-09

    申请号：EP98913120.0

    申请日：1998-03-27

    Applicant: Applied Spectral Imaging Ltd.

    Inventor： GARINI, Yuval ,  KATZIR, Nir ,  WINE, David ,  CABIB, Dario

    IPC: G01N33 ,  C12M1 ,  C12M3 ,  C12N15 ,  C12Q1 ,  G01J3 ,  G01N1 ,  G01N21 ,  G01N37 ,  G02B21 ,  G06K9

    CPC classification number: G01J3/12 ,  C12Q1/6816 ,  G01J3/02 ,  G01J3/1256 ,  G01J3/26 ,  G01J3/2823 ,  G01J3/4406 ,  G01J3/453 ,  G01J2003/2866 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/5005 ,  G01N33/56966 ,  G01N33/582 ,  G01N2021/6423 ,  G01N2021/6441 ,  G06K9/00127 ,  G06K9/0014 ,  G06K9/76

    Abstract: A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) using the pixels belonging to the other L-K chromosomes or portions of chromosomes for calculating the other L-K internal reference vectors, thereby finding all of the L internal reference vectors. A method for classification of L chromosomes or portions of chromosomes of a cell similarly painted using the above method for finding L internal reference vectors, and using the L reference vectors for classification of each of the pixels into one of L classification classes. And, images presenting color chromosomes.

    METHOD FOR INTERFEROMETER BASED SPECTRAL IMAGING OF MOVING OBJECTS
    117.
    发明公开
    METHOD FOR INTERFEROMETER BASED SPECTRAL IMAGING OF MOVING OBJECTS 失效
    对象的方法干涉测量光谱图表

    公开(公告)号：EP0874973A1

    公开(公告)日：1998-11-04

    申请号：EP97926494.0

    申请日：1997-05-12

    Applicant: Applied Spectral Imaging Ltd.

    Inventor： CABIB, Dario ,  BUCKWALD, Robert, A.

    IPC: G02B ,  A61B3 ,  G01B9 ,  G01J3

    CPC classification number: G01J3/45

    Abstract: A method for spatial registration and spectral correction for interferometer based spectral imaging which can be used to obtain spectral images of a moving object, the method comprising the steps of (a) using an interferometer based spectral imager for acquiring spatial and spectral information of the moving object; and (b) correcting the spatial and spectral information for movements of the moving object via a spatial registration and spectral correction procedures for obtaining corrected spatial and spectral information.

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