公开(公告)号：WO2023278847A1

公开(公告)日：2023-01-05

申请号：PCT/US2022/035963

申请日：2022-07-01

Applicant: FLUIDIGM CORPORATION

Inventor： SHEN, Zhongwei ,  HUKARI, Kyle Wisdom ,  CHEANG, Kum Hon

IPC: G02B21/06 ,  G01N31/22 ,  G01N21/00

Abstract: Systems and associated methods and techniques for illuminating and imaging a device, such as a microfluidic or microarray device, are described herein. An optical source that illuminates the planar surface at an oblique angle can be used with optical components, such as an offset optical shaping rod and a wedge prism, used to provide uniform illumination across the planar surface and allow the illumination to appropriately reach the target illumination area despite the geometric limitations imposed by the presence and position of imaging, microfluidic control, and/or thermal cycling components.

公开(公告)号：WO2021101893A1

公开(公告)日：2021-05-27

申请号：PCT/US2020/060892

申请日：2020-11-17

Applicant: FLUIDIGM CORPORATION

Inventor： SANDKUIJL, Daaf ,  CARPINO, Alan

IPC: G02B21/16 ,  G02B21/00 ,  G02B21/32 ,  G02B21/14

Abstract: A microscopy apparatus comprises a microscope comprising a stage configured to hold a tissue sample, a UV laser assembly configured to emit a UV laser beam to a viewing area of the tissue sample, and an IR laser assembly configured to emit an IR laser beam to the viewing area of the tissue sample. The UV and IR laser assemblies are oriented so as to emit the respective UV and IR laser beams in a same direction.

公开(公告)号：WO2020198614A1

公开(公告)日：2020-10-01

申请号：PCT/US2020/025296

申请日：2020-03-27

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： LI, Stephen K.H. ,  MAJONIS, Daniel ,  BARANOV, Vladimir ,  ORNATSKY, Olga ,  ALLO, Bedilu ,  BANDURA, Dmitry ,  THOM, Colin ,  STELZER, Greg ,  LOBODA, Alexander

IPC: A61K9/19 ,  G01N15/10 ,  G01N27/62 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532

Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.

公开(公告)号：WO2017106777A1

公开(公告)日：2017-06-22

申请号：PCT/US2016/067368

申请日：2016-12-16

Applicant: FLUIDIGM CORPORATION

Inventor： CHEN, Peilin

IPC: C12Q1/68

CPC classification number: B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00722 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301

Abstract: The present disclosure provides a "looping amplification" method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

Abstract translation: 本公开提供了“循环扩增”。 方法来增加核酸扩增的特异性。 这种增加的特异性有助于复用的程度比以前更高。

公开(公告)号：WO2013188748A1

公开(公告)日：2013-12-19

申请号：PCT/US2013/045848

申请日：2013-06-14

Applicant: FLUIDIGM CORPORATION

Inventor： LI, Nianzhen ,  UNGER, Marc

IPC: C12N5/10 ,  C12N5/02 ,  C12Q1/68 ,  C12M3/02

CPC classification number: C12N5/0618 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  C12M23/16 ,  C12N5/0619 ,  C12N15/111 ,  C12N2310/141 ,  C12N2320/12 ,  C12N2501/65 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2506/1307 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5026 ,  G01N33/57438

Abstract: This invention provides technology for transdifferentiating cells from one cell type to another. The cells are cultured with one or more vector-free gene regulator oligonucleotides concurrently or in succession, and then harvested when cell markers or the morphology of the culture shows that transdifferentiation is complete. Suitable gene regular oligonucleotides include microRNAs and messenger RNAs that encode a differentiation factor. Conditions for transdifferentiation can be optimized by dividing cells into different culture chambers of a microfluidic device. Cells are cultured with different additives in each chamber, and then compared. Transdifferentiated cells produced according to this invention can provide a consistent source of tissue for use in regenerative medicine.

Abstract translation: 本发明提供了将细胞从一种细胞类型转移到另一种细胞类型的技术。 细胞与一种或多种无载体的基因调节寡核苷酸同时或相继培养，然后当细胞标记物或培养物的形态显示转分化完成时收获细胞。 合适的基因正常寡核苷酸包括编码分化因子的微小RNA和信使RNA。 可以通过将细胞分成微流体装置的不同培养室来优化用于转分化的条件。 每个室中用不同的添加剂培养细胞，然后进行比较。 根据本发明生产的转分化细胞可提供用于再生医学的一致的组织来源。

公开(公告)号：WO2013130714A1

公开(公告)日：2013-09-06

申请号：PCT/US2013/028170

申请日：2013-02-28

Applicant: FLUIDIGM CORPORATION ,  FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

Inventor： FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

IPC: G01N15/10

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

Abstract translation: 描述了利用微流体的多单细胞捕获和处理的方法，系统和装置。 提供的工具和技术用于捕获，分配和/或操纵来自较大群体的细胞的各个细胞以及产生与每个单个细胞相关的遗传信息和/或反应。 可以使用不同的捕获配置来捕获单个细胞，然后在多室反应配置中处理每个单独的细胞。 一些实施方案可以提供已经从较大群体中划分的多个单个细胞的特异性靶扩增，全基因组扩增，全转录组扩增，实时PCR制备，拷贝数变异，预扩增，mRNA测序和/或单倍型 细胞。 一些实施例可以提供其他应用。 一些实施例可以被配置为用于对作为处理的一部分的各个单元或相关联的反应产物进行成像。 在某些情况下可以收获和/或进一步分析反应产物。

公开(公告)号：WO2012154876A1

公开(公告)日：2012-11-15

申请号：PCT/US2012/037155

申请日：2012-05-09

Applicant: FLUIDIGM CORPORATION ,  LIVAK, Kenneth J. ,  MYERS, Stacey ,  WANG, Xiaohui ,  WANG, Jun

Inventor： LIVAK, Kenneth J. ,  MYERS, Stacey ,  WANG, Xiaohui ,  WANG, Jun

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q2525/151

Abstract: The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction.

Abstract translation: 本发明提供了通过用核苷酸标签标记核苷酸序列来检测靶核苷酸序列的方法，提供了具有调节序列和核苷酸标签识别序列的融解温度Tm1的探针寡核苷酸; 在多核苷酸扩增反应中将探针寡核苷酸掺入标记的多核苷酸中，提供具有解链温度Tm2的调节性寡核苷酸，其包含与调节序列至少部分互补的序列片段，在PCR扩增中扩增标记的靶核酸序列 使用探针寡核苷酸作为引物的反应，检测扩增产物; 其中Tm1和Tm2高于与多核苷酸扩增反应相关的退火温度。

公开(公告)号：WO2010011852A1

公开(公告)日：2010-01-28

申请号：PCT/US2009/051568

申请日：2009-07-23

Applicant: FLUIDIGM CORPORATION ,  COHEN, David S.

Inventor： COHEN, David S.

IPC: C12Q1/68 ,  B01L3/00

CPC classification number: B29C41/02 ,  B01L3/502 ,  B01L3/502707 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  B29K2083/00 ,  B29L2009/00 ,  B29L2031/755 ,  B32B37/182 ,  B32B38/0008 ,  B32B2309/105 ,  B32B2383/00 ,  G01N30/6095

Abstract: METHOD AND SYSTEM FOR MANUFACTURING INTEGRATED FLUIDIC CHIPS ABSTRACT OF THE DISCLOSURE An integrated fluidic chip includes a substrate defined by a lateral surface area greater than 28 square inches. The integrated fluidic chip also includes a first elastomeric layer having a mold surface and a top surface. The mold surface of the first elastomeric layer is joined to a portion of the substrate. The first elastomeric layer includes a plurality of first channels extending normally from the substrate to a first dimension inside the first elastomeric layer. The integrated fluidic chip further includes a second elastomeric layer having a mold surface and a top surface. The mold surface of the second elastomeric layer is joined to at least a portion of the top surface of the first elastomeric layer.

Abstract translation: 用于制造集成流体液晶的方法和系统本发明摘要集成流体芯片包括由大于28平方英寸的侧表面积限定的基板。 集成流体芯片还包括具有模具表面和顶表面的第一弹性体层。 第一弹性体层的模具表面连接到基底的一部分。 第一弹性体层包括多个第一通道，其从基底正常延伸到第一弹性体层内的第一尺寸。 集成流体芯片还包括具有模具表面和顶表面的第二弹性体层。 第二弹性体层的模具表面连接到第一弹性体层的顶表面的至少一部分。

公开(公告)号：WO2009126826A1

公开(公告)日：2009-10-15

申请号：PCT/US2009/040104

申请日：2009-04-09

Applicant: FLUIDIGM CORPORATION ,  FACER, Geoffrey ,  FOWLER, Brian ,  QUAN, Emerson, Cheung ,  UNGER, Marc

Inventor： FACER, Geoffrey ,  FOWLER, Brian ,  QUAN, Emerson, Cheung ,  UNGER, Marc

IPC: B81B3/00

CPC classification number: F16K99/0015 ,  B01F5/02 ,  B01F13/0059 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0627 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0638 ,  B33Y80/00 ,  C12Q1/686 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N2021/0346 ,  Y10T137/0329 ,  Y10T137/2164 ,  Y10T137/2169 ,  Y10T137/2559 ,  Y10T137/2655 ,  Y10T137/2688 ,  Y10T137/87249

Abstract: Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.

Abstract translation: 多级微流体装置包括一个控制管线，可同时为样品和试剂管线起动阀门。 微流体装置被配置为在第二室中的第一室和第二试剂中含有第一试剂，其中第一试剂和第二试剂中的任一种或两者以所需或选择的压力包含。 微流体装置的操作包括将第二试剂从第二室传输到第一室，用于与第一试剂混合或接触。 诸如通道，阀，腔室的微流体装置特征可以至少部分地包含，嵌入或由弹性体块的一个或多个层或层形成或形成。

公开(公告)号：WO2008089493A2

公开(公告)日：2008-07-24

申请号：PCT/US2008/051728

申请日：2008-01-22

Applicant: FLUIDIGM CORPORATION ,  COHEN, David, S. ,  WANG, Jing ,  MAY, Andrew, P. ,  JONES, Robert, C. ,  NASSEF, Hany

Inventor： COHEN, David, S. ,  WANG, Jing ,  MAY, Andrew, P. ,  JONES, Robert, C. ,  NASSEF, Hany

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/5025 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0638 ,  B01L2400/0655 ,  B33Y80/00 ,  F16K99/0001 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/0318 ,  Y10T137/85938

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract translation: 新的高密度微流体装置和方法提供流体体积的精确计量和计量体积的有效混合。 将第一溶液引入与反应室流体连通的流动通道的段中。 第二溶液流过该段，使得第一溶液移入反应室，并且第二溶液的体积进入该室。 然后可以分离室，并且可以启动和/或检测室内的反应。 遗传分析的高通量方法可以比以前更高的精度进行。