Abstract:
본 발명은 라텍스 비드를 이용한 네오스포라병의 진단 시약 및 진단 키트에 관한 것이다. 보다 구체적으로 본 발명은 네오스포라 캐니눔( Neospora
caninum ) 항원으로 감작시킨 라텍스 비드를 포함하는 네오스포라병 진단 시약, 이를 포함하는 네오스포라병 진단 키트 및 이를 이용한 네오스포라병 진단 방법에 관한 것이다. 본 발명에 따른 네오스포라 원충 항원 및 라텍스 비드를 이용한 진단 시약은 네오스포라병에 대한 정확한 진단을 고가의 장비없이 간편하게 수행할 수 있는 효과가 있다. 네오스포라병, 라텍스 비드, 진단
Abstract:
A diagnosis kit of neosporosis using immunochromatography is provided to simply and economically diagnose neosporosis without expensive equipment. A diagnosis kit using immunochromatography comprises a neospora canium antigen which is adhered on a film. The antigen is Neospora caninum Kr2 strain. The film is a nitrocellulose film. The diagnosis further comprises anti-bovine immunoglobulin G on the film.
Abstract:
A feed additive using a coptic rhizome and forsythia fruit extract is provided to accelerate the growth of animal, to reinforce an immune function and to prevent diseases, by adding predetermined amount of extracts obtained from Forsythiae Fructus and Coptidis Rhizoma and supplying it to domestic animals, thereby substituting antibiotics. A feed additive using a coptic rhizome and forsythia fruit extract comprises Coptis japonica extract 50 weight%, forsythia extract 10 weight%, and dextrose 30 weight% and Glycyrrhiza extract 10 weight%.
Abstract:
본 발명은 엔테로코커스 에스피 58 (KACC 91099) 및 이의 용도에 관한 것으로서, 상기 균주는 내산성, 내담즙성 및 생체 안전성이 인정되고, 병원성 세균 억제활성을 가지며, 장내에서 독립된 균총(Niche)으로서의 활성과 이에 근거한 길항 작용을 통한 병원성 균총의 증식을 감소시켜 질병을 예방하는 효과가 있다.
Abstract:
PURPOSE: An immunoglobulin yolk according to a bee virus is provided to be used as a controlling method of environment-friendly bee virus by inducing an immune reaction in the bee and to replace chemical controlling method or a conventional post antigen detecting method. CONSTITUTION: A manufacturing method of immunoglobulin yol according to a bee virus comprises 1) a step of inoculating the bee virus as an antigen to an egg, 2) a step fo collecting yolk from the egg, and 3) a step of separating the immunoglobulin yolk according to the bee virus from the yolk. The inoculation is performed 0.1ml to 0.5ml for the first inoculation and for two weeks apart, 2-4 times are inoculated. A composition for the bee virus prevention comprises the bee virus immunoglobulin yolk.
Abstract:
PURPOSE: A culturing method of bee virus using a bee larva is provided to be usefully used on screening effective treatment by evaluating effect of the therapeutic agent according to the various bee virus using effectively cultured bee virus which was hard to culture in-vitro. CONSTITUTION: A culturing method of bee virus is to infect the bee virus to artificially cultivated bee larva. The articifial culturing is performed on the bee larva at 35-40 degree Celsius and 80-90% humidity. The culturing comprises 5-10% of D-glucose, 5-10% of D-fructose, 1-3% of enzyme extract, 25-35% of grace medium, and 80-100 micro liters of solution comprised of 40-50% of royal jelly. A bee virus treating material screening method using the artificially cultured larva comprises 1) a step of artificially culturing the bee larva in-vitro, 2) a step of culturing a separated bee virus to the larva from 1), and 3) a step of measuring concentration of virus after treating the bee virus therapeutic agent. [Reference numerals] (AA) Virus infected group; (BB) Thymol and chlorine dioxide treated group; (CC) Virus non-infected group; (DD) Survival rate (%); (EE) Cultivated days
Abstract:
본 발명은 면역크로마토그래피를 이용한 네오스포라병의 진단 키트에 관한 것이다. 보다 구체적으로는, 세포 배양된 국내 분리주 네오스포라 원충의 항원을 이용하여 면역크로마토그래피법을 이용한 혈청학적 진단법에 관한 것으로, 본 발명에 따르면, 막에 항원을 결합시킨 후 가검 혈청과의 반응여부를 육안적으로 관찰하여 판단하기 때문에, 본 발명에 의해 고가의 장비를 필요로 하지 않는 간편하고 경제적 진단법을 제공할 수 있다. 소, 유·사산, 네오스포라병, 면역크로마토그래피, 진단방법
Abstract:
본 발명은 엔테로코커스 에스피 58 (KACC 91099) 및 이의 용도에 관한 것으로서, 상기 균주는 내산성, 내담즙성 및 생체 안전성이 인정되고, 병원성 세균 억제활성을 가지며, 장내에서 독립된 균총(Niche)으로서의 활성과 이에 근거한 길항 작용을 통한 병원성 균총의 증식을 감소시켜 질병을 예방하는 효과가 있다.
Abstract:
PURPOSE: A fusion protein having enhanced immunogenicity to bovine theileriosis, a preparation process thereof, and the use thereof are provided, thereby preventing the infection of bovine theileriosis. CONSTITUTION: A fusion protein having enhanced immunogenicity to bovine theileriosis is prepared by fusion of heat shock protein(HSP) with the amino-terminal of the surface antigen p33 of Theileria sergenti, wherein Theileria sergenti is Korean isolated Theileria sergenti, Sungwhan stock; the surface antigen p33 has the nucleotide sequence of SEQ ID NO: 1; and the heat shock protein(HSP) has the nucleotide sequence of SEQ ID NO: 2. A process for preparing the fusion protein having enhanced immunogenicity to bovine theileriosis comprises the steps of: constructing a recombinant vector containing the gene encoding the fusion protein of heat shock protein(HSP) and the surface antigen p33 of Theileria sergenti; preparing a transformed E. coli with the recombinant vector; and culturing the transformed E. coli.
Abstract translation:目的:提供具有增强的牛鳞状细胞病毒免疫原性的融合蛋白及其制备方法及其用途,从而防止牛皮肤病的感染。 构成:通过将热休克蛋白(HSP)与Theileria sergenti的表面抗原p33的氨基末端融合制备具有增强的牛鳞状细胞病毒免疫原性的融合蛋白,其中Theileria sergenti是韩国分离的Theileria sergenti,Sungwhan stock; 表面抗原p33具有SEQ ID NO:1的核苷酸序列; 热休克蛋白(HSP)具有SEQ ID NO:2的核苷酸序列。一种制备具有增强的牛鳞状细胞病免疫原性的融合蛋白的方法,包括以下步骤:构建含有编码热能融合蛋白的基因的重组载体 休克蛋白(HSP)和沙门氏菌的表面抗原p33; 用重组载体制备转化的大肠杆菌; 并培养转化的大肠杆菌。