라텍스 비드를 이용한 네오스포라병의 진단 시약 및 진단키트
    11.
    发明授权
    라텍스 비드를 이용한 네오스포라병의 진단 시약 및 진단키트 有权
    诊断代理和使用LATEX珠子的新生儿手套

    公开(公告)号:KR100951056B1

    公开(公告)日:2010-04-07

    申请号:KR1020070109076

    申请日:2007-10-29

    Abstract: 본 발명은 라텍스 비드를 이용한 네오스포라병의 진단 시약 및 진단 키트에 관한 것이다. 보다 구체적으로 본 발명은 네오스포라 캐니눔(
    Neospora

    caninum ) 항원으로 감작시킨 라텍스 비드를 포함하는 네오스포라병 진단 시약, 이를 포함하는 네오스포라병 진단 키트 및 이를 이용한 네오스포라병 진단 방법에 관한 것이다.
    본 발명에 따른 네오스포라 원충 항원 및 라텍스 비드를 이용한 진단 시약은 네오스포라병에 대한 정확한 진단을 고가의 장비없이 간편하게 수행할 수 있는 효과가 있다.
    네오스포라병, 라텍스 비드, 진단

    황련 및 연교추출물을 이용한 사료첨가제의 추출방법
    13.
    发明公开
    황련 및 연교추출물을 이용한 사료첨가제의 추출방법 有权
    使用酚醛树脂和果汁提取物及其提取方法的饲料添加剂

    公开(公告)号:KR1020080095988A

    公开(公告)日:2008-10-30

    申请号:KR1020070040731

    申请日:2007-04-26

    CPC classification number: A23K10/30 A23K40/00

    Abstract: A feed additive using a coptic rhizome and forsythia fruit extract is provided to accelerate the growth of animal, to reinforce an immune function and to prevent diseases, by adding predetermined amount of extracts obtained from Forsythiae Fructus and Coptidis Rhizoma and supplying it to domestic animals, thereby substituting antibiotics. A feed additive using a coptic rhizome and forsythia fruit extract comprises Coptis japonica extract 50 weight%, forsythia extract 10 weight%, and dextrose 30 weight% and Glycyrrhiza extract 10 weight%.

    Abstract translation: 提供使用科普特根和连翘果提取物的饲料添加剂,通过加入预定量的连翘和黄连提取物提取动物,增加免疫功能,增强免疫功能,预防疾病,并将其提供给家畜, 从而代替抗生素。 使用科普特根和连翘果提取物的饲料添加剂包括50重量%的黄连提取物,10重量%的连翘提取物和30重量%的葡萄糖和10重量%的甘草提取物。

    벌 바이러스에 대한 난황항체 및 이의 제조방법
    16.
    发明公开
    벌 바이러스에 대한 난황항체 및 이의 제조방법 有权
    用于乙型病毒的EGG-YOLK抗体及其生产方法

    公开(公告)号:KR1020130077295A

    公开(公告)日:2013-07-09

    申请号:KR1020110145925

    申请日:2011-12-29

    Abstract: PURPOSE: An immunoglobulin yolk according to a bee virus is provided to be used as a controlling method of environment-friendly bee virus by inducing an immune reaction in the bee and to replace chemical controlling method or a conventional post antigen detecting method. CONSTITUTION: A manufacturing method of immunoglobulin yol according to a bee virus comprises 1) a step of inoculating the bee virus as an antigen to an egg, 2) a step fo collecting yolk from the egg, and 3) a step of separating the immunoglobulin yolk according to the bee virus from the yolk. The inoculation is performed 0.1ml to 0.5ml for the first inoculation and for two weeks apart, 2-4 times are inoculated. A composition for the bee virus prevention comprises the bee virus immunoglobulin yolk.

    Abstract translation: 目的:提供一种根据蜂病毒的免疫球蛋白,通过诱导蜜蜂中的免疫反应,替代化学治疗方法或常规的后抗原检测方法,将其用作环境友好的蜂病毒的控制方法。 构成:根据蜂病毒的免疫球蛋白的制造方法包括:1)将作为抗原的蜂病毒接种于卵的步骤,2)从卵收集蛋黄的步骤,3)分离免疫球蛋白的步骤 根据来自蛋黄的蜂病毒的蛋黄。 接种0.1ml至0.5ml进行第一次接种,间隔两周,接种2-4次。 用于预防蜂病毒的组合物包括蜂病毒免疫球蛋白蛋黄。

    벌 애벌레를 이용한 벌 바이러스의 배양 방법 및 이를 이용한 벌 바이러스 치료물질 스크리닝 방법
    17.
    发明公开
    벌 애벌레를 이용한 벌 바이러스의 배양 방법 및 이를 이용한 벌 바이러스 치료물질 스크리닝 방법 无效
    使用BEE LARVA的BEE病毒培养方法和使用其的BEE病毒的治疗剂的筛选方法

    公开(公告)号:KR1020130077232A

    公开(公告)日:2013-07-09

    申请号:KR1020110145828

    申请日:2011-12-29

    Abstract: PURPOSE: A culturing method of bee virus using a bee larva is provided to be usefully used on screening effective treatment by evaluating effect of the therapeutic agent according to the various bee virus using effectively cultured bee virus which was hard to culture in-vitro. CONSTITUTION: A culturing method of bee virus is to infect the bee virus to artificially cultivated bee larva. The articifial culturing is performed on the bee larva at 35-40 degree Celsius and 80-90% humidity. The culturing comprises 5-10% of D-glucose, 5-10% of D-fructose, 1-3% of enzyme extract, 25-35% of grace medium, and 80-100 micro liters of solution comprised of 40-50% of royal jelly. A bee virus treating material screening method using the artificially cultured larva comprises 1) a step of artificially culturing the bee larva in-vitro, 2) a step of culturing a separated bee virus to the larva from 1), and 3) a step of measuring concentration of virus after treating the bee virus therapeutic agent. [Reference numerals] (AA) Virus infected group; (BB) Thymol and chlorine dioxide treated group; (CC) Virus non-infected group; (DD) Survival rate (%); (EE) Cultivated days

    Abstract translation: 目的:提供使用蜜蜂幼虫的蜂毒病毒培养方法,通过使用有效培养的体外培养的蜜蜂病毒评估各种蜂病毒治疗剂的效果,有效地用于筛选有效的治疗。 构成:蜜蜂病毒的培养方法是将蜜蜂病毒感染到人工培养的蜜蜂幼虫。 在35-40摄氏度和80-90%湿度的蜜蜂幼虫进行表达培养。 培养包括5-10%的D-葡萄糖,5-10%的D-果糖,1-3%的酶提取物,25-35%的宽带培养基和80-100微升的溶液组成的40-50 %的蜂王浆。 使用人造培养的幼虫的蜂病毒处理材料筛选方法包括1)在体外人工培养蜜蜂幼体的步骤,2)从1)培养分离的蜜蜂病毒至幼虫的步骤,以及3)步骤 治疗蜂病毒治疗剂后测定病毒浓度。 (附图标记)(AA)病毒感染组; (BB)百里酚和二氧化氯处理组; (CC)病毒非感染组; (DD)生存率(%); (EE)耕种日

    소 타일레리아병에 대한 면역원성이 증진된 융합단백질, 및 그의 제조방법 및 용도
    20.
    发明公开
    소 타일레리아병에 대한 면역원성이 증진된 융합단백질, 및 그의 제조방법 및 용도 有权
    具有增强免疫原性的熔化蛋白,其制备方法及其用途的融合蛋白

    公开(公告)号:KR1020030053857A

    公开(公告)日:2003-07-02

    申请号:KR1020010083880

    申请日:2001-12-24

    Abstract: PURPOSE: A fusion protein having enhanced immunogenicity to bovine theileriosis, a preparation process thereof, and the use thereof are provided, thereby preventing the infection of bovine theileriosis. CONSTITUTION: A fusion protein having enhanced immunogenicity to bovine theileriosis is prepared by fusion of heat shock protein(HSP) with the amino-terminal of the surface antigen p33 of Theileria sergenti, wherein Theileria sergenti is Korean isolated Theileria sergenti, Sungwhan stock; the surface antigen p33 has the nucleotide sequence of SEQ ID NO: 1; and the heat shock protein(HSP) has the nucleotide sequence of SEQ ID NO: 2. A process for preparing the fusion protein having enhanced immunogenicity to bovine theileriosis comprises the steps of: constructing a recombinant vector containing the gene encoding the fusion protein of heat shock protein(HSP) and the surface antigen p33 of Theileria sergenti; preparing a transformed E. coli with the recombinant vector; and culturing the transformed E. coli.

    Abstract translation: 目的:提供具有增强的牛鳞状细胞病毒免疫原性的融合蛋白及其制备方法及其用途,从而防止牛皮肤病的感染。 构成:通过将热休克蛋白(HSP)与Theileria sergenti的表面抗原p33的氨基末端融合制备具有增强的牛鳞状细胞病毒免疫原性的融合蛋白,其中Theileria sergenti是韩国分离的Theileria sergenti,Sungwhan stock; 表面抗原p33具有SEQ ID NO:1的核苷酸序列; 热休克蛋白(HSP)具有SEQ ID NO:2的核苷酸序列。一种制备具有增强的牛鳞状细胞病免疫原性的融合蛋白的方法,包括以下步骤:构建含有编码热能融合蛋白的基因的重组载体 休克蛋白(HSP)和沙门氏菌的表面抗原p33; 用重组载体制备转化的大肠杆菌; 并培养转化的大肠杆菌。

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