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11.发明授权인체 리포코틴을 E. coli에서 생산시 가용성인 상태로 생산 정제하는 방법 및 인체 리포코틴 cDNA 서열을 함유하는 벡터 失效当在大肠杆菌中生产时，以可溶状态生产和纯化人脂皮质蛋白的方法和生产含有人脂皮质蛋白cDNA序列的载体的方法
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Patent Agency Ranking
公开(公告)号：KR1019890003715B1
公开(公告)日：1989-09-30
申请号：KR1019870006560
申请日：1987-06-27
Applicant: 한국과학기술연구원
IPC: C12N15/00
Abstract: A fragment having -300 base pairs was obtained from a plasmid, PILM 7, cut by XbaI and PvuII. On the other hand, a fragment having - 4,500 base pairs was obtained from aplasmid, pNK2, treated with XbaI and PvuII. Both of the fragments were ligated and inserted into a proper strain to get pNKM 21. pNKM 21 was inserted into M 5248 strain to get KCTC 8258 P strain followed by culturing it to give a interleukin-2 contg. serine at 125-position instead of cystein.
Abstract translation: 从由XbaI和PvuII切割的质粒PILM7获得具有-300个碱基对的片段。 另一方面，从用XbaI和PvuII处理的质粒pNK2获得具有-4,500个碱基对的片段。 将两个片段连接并插入适当的菌株中以获得pNKM21。将pNKM 21插入M 5248菌株中以获得KCTC 8258P菌株，然后培养以产生白细胞介素-2对照。 丝氨酸位于125位，而不是半胱氨酸。
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公开(公告)号：KR1019950009839B1
公开(公告)日：1995-08-29
申请号：KR1019920007594
申请日：1992-05-04
Applicant: 한국과학기술연구원
IPC: C12N15/12
Abstract: The plasmids pHT2 8505P (I) and pHT21 8506P (II) for human lipocortin-1 (III) are prepared by (1) isolating gene of (III) from plasmid pLCI-S KCTC 84189 (KR Appl. 89-223), (2) and adding trc promoter for IPTG inducing. (III) which inhibit for phospholipase A2 to prostaglandin and arachidonic acid formation is useful for anti-inflammatory and rheumatoid fever treatment.
Abstract translation: 通过（1）从质粒pLCI-S KCTC 84189（KR Appl.89-223）分离（III）的基因（（III））分离人脂细胞素-1（III）的质粒pHT2 8505P（I）和pHT21 8506P（II） 2）并加入诱导IPTG的trc启动子。 抑制磷脂酶A2对前列腺素和花生四烯酸形成的抑制剂（III）可用于抗炎和类风湿热治疗。
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