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公开(公告)号：JPS6367144A
公开(公告)日：1988-03-25
申请号：JP21143386
申请日：1986-09-10
Applicant: AGENCY IND SCIENCE TECHN , MITSUBISHI MONSANTO CHEM
Inventor： KODAMA AKIRA , SAKAI TSUKASA , TSUDA KEISHIRO , YOKOYAMA KEIJI
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公开(公告)号：JPS6315152A
公开(公告)日：1988-01-22
申请号：JP15943286
申请日：1986-07-07
Applicant: AGENCY IND SCIENCE TECHN
Inventor： IIJIMA SEIICHIRO , MIZUTANI FUMIO , TANAKA YOSHIO , TANABE GIICHI , TSUDA KEISHIRO
IPC: G01N27/333 , G01N27/30 , G01N27/48
Abstract: PURPOSE:To easily discriminate the anion contained in an aqueous solution, by applying potential scanning to a polydivinyl ferrocene membrane in the aqueous solution to measure the peak potential of a current-potential curve. CONSTITUTION:A conductor (acting electrode) 3 covered with a polydivinyl ferrocene membrane, a reference electrode 4 and an opposed electrode 5 are inserted in an aqueous electrolyte solution 2. Next, a potentiostat 6 and a function generator 7 are combined to perform potential scanning at a predetermined scanning speed. The obtained current-potential curve is recorded on an X-Y recording needle, and anode peak potential and cathode peak potential are read. Since said potentials show different values by the anion contained in the aqueous solution, the kind of the anion contained in an unknown specimen can be discriminated from said anode and cathode peak potentials.
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公开(公告)号：JPS62228168A
公开(公告)日：1987-10-07
申请号：JP5181586
申请日：1986-03-10
Applicant: AGENCY IND SCIENCE TECHN
Inventor： YOKOYAMA NAOKI , NAKAO YUKIMICHI , OHASHI SHINICHI , KAERIYAMA KYOJI , TSUDA KEISHIRO , SUDA MASAO , IMAI TOMOYUKI
Abstract: PURPOSE:To safely and economically detect protein immobilized on a membrane with high sensitivity, by a simple means wherein the membrane having protein immobilized thereon is treated with a palladium colloid solution with predetermined pH stabilized by a surfactant and a base metal is applied to the treated membrane by an electroless plating. CONSTITUTION:A membrane such as a nitrocellulose having protein immobilized thereon is treated with a palladium colloid solution with pH 5 or less stabilized by an anionic surfactant such as sodium dodecylbenzenesulfonate and a base metal is subsequently applied to the treated membrane by electroless plating. The content of palladium in the palladium colloid solution is 0.01-30mmol/l and the content of the surfactant is 0.001-5wt%. The pH of said colloid solution is pref. adjusted by an acid such as acetic acid or hydrochloric acid. The membrane having protein immobilized thereon is immersed in the palladium colloid solution to be stained and, thereafter, the electroless plating of the base metal is applied to said membrane to enhance detection sensitivity.
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公开(公告)号：JPS62181782A
公开(公告)日：1987-08-10
申请号：JP2533386
申请日：1986-02-06
Applicant: AGENCY IND SCIENCE TECHN
Inventor： SHIMIZU TAKASHI , SUZUKI YASUZO , TANAKA YOSHIO , TSUDA KEISHIRO
Abstract: PURPOSE:To increase the enzymic activity, by the presence of a given amount of dimethyl sulfoxide in a reaction solution in enzymic reaction using pyruvic acid kinase. CONSTITUTION:Dimethyl solfoxide in a volume to give a concentration within the range of 5-20vol% is added to a reaction solution in enzymic reaction using pyruvic acid kinase. The above-mentioned enzymic reaction is carried out at 6-9pH and 20-37 deg.C using further 5-200mmol/l potassium chloride and 1-10mmol/l magnesium chloride as additives. According to this method, since the enzymic activity of the pyruvic acid kinase can be increased by about 50%, the amount of the pyruvic acid kinase to be used can be remarkably reduced in various enzymic reactions and the method is economical.
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公开(公告)号：JPS62115287A
公开(公告)日：1987-05-26
申请号：JP25566085
申请日：1985-11-14
Applicant: AGENCY IND SCIENCE TECHN
Inventor： IWAKURA MASAHIRO , FURUSAWA KIYOTAKA , OHASHI SHINICHI , TSUDA KEISHIRO
Abstract: PURPOSE:A recombinant plasmid containing a gene to code 1st-29th peptide fragments among bovine growth hormone release factor peptide having secretion adjusting activity of growth hormone. CONSTITUTION:Novel recombinant plasmid pGRF29-28 consisting of 5406 pairs of bases having DNA sequence partially shown by formula II, a recombinant plasmid which can provide a host with ampicillin resistance, codes a peptide wherein a peptide sequence consisting of 6 amino acids of methionine-isoleucine- isoleucine-glutamic acid-glycine-alginine is fused to an amino end side of 1st-29th peptide fragment among bovine growth hormone release factor peptide by cleavage with restriction enzyme BclI and can free a DNA sequence consisting of 107 pairs of bases shown by formula I.
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Patent Agency Ranking
公开(公告)号：JPS6297687A
公开(公告)日：1987-05-07
申请号：JP23740585
申请日：1985-10-25
Applicant: AGENCY IND SCIENCE TECHN , HITACHI LTD
Inventor： ASAI MICHIHIKO , SAKAI TSUKASA , TSUDA KEISHIRO , TAKEMOTO KAZUNARI , KITO MAKOTO , KUROIWA MINORU , KOMATSU TAKASHI
Abstract: PURPOSE:To reduce the elution of an org. substance, by applying plasma treatment to the inside surface of each of the piping between apparatuses and the piping up to an area using ultrapure water to form a modified layer to said inside surface. CONSTITUTION:Primary pure water Wa supplied is subjected to sterilization treatment in an ultraviolet sterilization apparatus 1 and introduced into an ion exchange apparatus 2 through piping 4A to remove the dissolved ion in the pure water Wa. The pure water Wa after the removal of the ion is introduced into an ultrafiltration apparatus 3 through piping 4B and fine particles in the pure water Wa are removed to obtain ultrapure water Wb which is, in turn, supplied to a use area through piping 4C. Plasma treatment is applied to the inner surface of the piping main body 5 of each of the pipings 4A-4C by Ar-gas to form a modified layer 6. By this method, ultrapure water can be obtained.
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公开(公告)号：JPS61271990A
公开(公告)日：1986-12-02
申请号：JP11369285
申请日：1985-05-27
Applicant: AGENCY IND SCIENCE TECHN
Inventor： IWAKURA MASAHIRO , FURUSAWA KIYOTAKA , TSUDA KEISHIRO
Abstract: PURPOSE:A DNA sequence, consisting of a specific base sequence and containing a gene of an enzyme capable of catalyzing a reaction for reducing dihydrofolic acid and producing tetrahydrofolic acid. CONSTITUTION:A DNA sequence expressed by the formula. The DNA sequence consists of a part coding dihydrofolic acid reductase protein of E. coli (sequence A at the 1- - 477-position), part on the upstream side thereof (sequence B at the -56- - -1-position) and part on the downstream side thereof (sequence C consisting of TAA at the 478- - 480-translation stopping number). The sequences (A) and (C) are the same as the sequence derived from a chromosome DNA of E. coli, and the sequence (B) is derived from a chemically synthesized DNA. The sequence (B) consists of a cleavage site of a restriction enzyme, a site of translation stopping group corresponding to the reading frame of three gene translations and ribosome linking sites for E. coli (DNA sequence of AGGA) and for B. subtilis (DNA sequence of AAAGGAGG).
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公开(公告)号：JPS61226637A
公开(公告)日：1986-10-08
申请号：JP6809885
申请日：1985-03-30
Applicant: AGENCY IND SCIENCE TECHN
Inventor： MIZUTANI FUMIO , TANABE GIICHI , TSUDA KEISHIRO
Abstract: PURPOSE:To measure rapidly, easily and accurately the permeability of the film for a special chemical species in the aqueous solution including many components such as a fermentation liquid and a body fluid by obtaining the ratio of the time change of the response electric current output of the enzyme electrode and estimating the ratio of the time change of the density of the chemical type to transmit the film. CONSTITUTION:Through a film 1 to measure the permeability and the diffusion constant for a certain chemical species two aqueous solution phases 2 and 3 exist. Into the aqueous solution phase 3, the enzyme electrode is inserted in which a film 5 formed by fixing the oxidizing enzyme with the above- mentioned chemical species as one of the substrates at the surface of the enzyme electrode and the hydrogen peroxide electrode 4. The output electric current from the enzyme electrode is measured by an ammeter 6 and recorded by a recorder 7.
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公开(公告)号：JPS6387981A
公开(公告)日：1988-04-19
申请号：JP23400386
申请日：1986-09-30
Applicant: AGENCY IND SCIENCE TECHN
Inventor： IWAKURA MASAHIRO , KOKUBU TOMOKUNI , FURUSAWA KIYOTAKA , TSUDA KEISHIRO
Abstract: PURPOSE:To provide a recombinant plasmid containing integrated gene of dihydrofolic acid reductase (DHFR)-leucine encephaline fused protein, stably replicable in E.coli and capable of imparting said host with trimethoprim resistance and ampicillin resistance. CONSTITUTION:Plasmid pBSDHFR1 is cut with restriction enzymes EcoRI and PstI and separated by 1% agarose gel electrophoresis to obtain a small fragment DNA. Separately, plasmid pBR322 is cut with restriction enzyme BamHI and Pst and separated by 1% agarose gel electrophoresis to obtain a large fragment DNA. The DNA of formula I and formula II are synthesized, the 5'-terminal is converted to phosphoric acid group and the product is annealed to obtain a DNA coding leucine encephaline. The above 3 kinds of DNA molecules are linked together, introduced into E.coli C600 strain and cultured. The objective recombinant DNA containing the sequence of formula III coding a DHFR-leucine encephaline fused protein can be separated from the obtained colony.
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公开(公告)号：JPS6328394A
公开(公告)日：1988-02-06
申请号：JP17045986
申请日：1986-07-19
Applicant: AGENCY IND SCIENCE TECHN
Inventor： IWAKURA MASAHIRO , TSUDA KEISHIRO
Abstract: PURPOSE:The titled plasmid, obtained by replacing a dihydrofolate reductase gene part of plasmid pTP64-1 with a dihydrofolate reductase gene of Bacillus subtilis, stably replicable in a host and capable of imparting trimethoprim and ampicillin resistance. CONSTITUTION:A plasmid pTP64-1 is cleaved with a restriction enzyme to replace a dihydrofolate reductase part with dihydrofolate reductase of Bacillus subtilis to give the aimed plasmid pBSDHFR1. The resultant novel recombinant plasmid can be stably replicated in Escherichia coli (E. coli) to impart trimethoprim and ampicillin resistance to the E. coli which is a host. The plasmid has a size of 5504 base pairs and DNA sequence shown in the figure (No.501 and thereafter are omitted) and is useful in the fields of microbial, pharmaceutical industries.
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