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    MICROSTRUCTURES FOR THE MANIPULATION OF FLUID SAMPLES
    12.
    发明申请
    MICROSTRUCTURES FOR THE MANIPULATION OF FLUID SAMPLES 审中-公开
    用于操纵流体样本的微结构

    公开(公告)号：WO9909042A3

    公开(公告)日：1999-05-06

    申请号：PCT/US9816870

    申请日：1998-08-13

    Applicant: CEPHEID

    Inventor： CHRISTEL LEE ALLAN ,  KOVACS GREGORY T A ,  MCMILLAN WILLIAM A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  POURAHMADI FARZAD

    IPC: G01N31/20 ,  B01D15/08 ,  B01F5/04 ,  B01F5/06 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C07H1/00 ,  C07H21/00 ,  G01N1/34 ,  G01N27/447 ,  G01N33/53 ,  G01N37/00 ,  C12M1/26

    CPC classification number: B01L3/502753 ,  B01F5/0403 ,  B01F5/0645 ,  B01F13/0059 ,  B01J19/0093 ,  B01L3/502707 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/086 ,  C07H1/00 ,  G01N1/405

    Abstract: The invention presents a microfluidic device and method for separating a desired material, such as nucleic acid, from other materials in a fluid sample. In a preferred embodiment, the device comprises a microfabricated chip (20) having an inlet port (28), an outlet port (30), and an extraction chamber (26) in fluid communication with the ports. The chamber (26) has internal attachment surfaces for capturing the desired material from the fluid sample as the sample flows continuously through the chamber. The captured material may then be eluted by forcing an elution fluid to flow through the chamber (26), thus releasing the material from the internal surfaces into the elution fluid. The flow-through design of the device allows target material from a relatively large volume of fluid sample to be concentrated into a much smaller volume of elution fluid. The internal surfaces are preferably formed by an array of columns (32) integrally formed with a wall of the chamber (26) and extending into the chamber (26). The collumns (32) provide a large surface area for capturing the desired material. The device also preferably includes an integrated heater (34) for increasing elution efficiency.

    Abstract translation: 本发明提出了一种微流体装置和方法，用于从流体样本中的其他材料中分离出所需材料，如核酸。 在一个优选实施例中，该装置包括具有与端口流体连通的入口端口（28），出口端口（30）和抽取腔室（26）的微制造芯片（20）。 腔室（26）具有内部连接表面，用于在样品连续流过腔室时从流体样品中捕获所需材料。 然后可以通过迫使洗提流体流过室（26）来洗提捕获的材料，从而将材料从内表面释放到洗提流体中。 该装置的流通式设计允许来自相对较大体积的流体样品的目标物质被浓缩成小得多的洗脱液体积。 内表面优选由与腔室（26）的壁整体形成并延伸到腔室（26）中的一列柱（32）形成。 柱（32）为捕获所需材料提供了大的表面积。 该设备还优选包括用于提高洗脱效率的集成加热器（34）。

    17.
    发明专利
    未知

    公开(公告)号：DK1179585T3

    公开(公告)日：2008-11-10

    申请号：DK01118242

    申请日：1998-12-24

    Applicant: CEPHEID

    Inventor： POURAHMADI FARZAD ,  CHING JESUS ,  CHANG RONALD ,  PETERSEN KURT E ,  MCMILLIAN WILLIAM A ,  CHRISTEL LEE A ,  KOVACS GREGORY T ,  NORTHRUP M ALLEN

    IPC: B01J19/00 ,  G01N31/20 ,  B01D11/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/33 ,  C12M1/34 ,  C40B60/14 ,  G01N1/28 ,  G01N30/60 ,  G01N33/50 ,  G01N35/08 ,  G01N37/00

    Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g. filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid. A preferred embodiment relates to a device for lysing components of a fluid sample, the device comprising inter alia, an ultrasonic transducer.

    20.
    发明专利
    未知

    公开(公告)号：DE60039640D1

    公开(公告)日：2008-09-04

    申请号：DE60039640

    申请日：2000-12-15

    Applicant: CEPHEID

    Inventor： CHANG RONALD ,  DORITY DOUGLAS B ,  CHRISTEL LEE A ,  PETERSEN KURT E

    IPC: B01L7/00 ,  B01J19/00 ,  B01L3/00 ,  B01L9/00 ,  C12Q1/68 ,  F28F13/00 ,  G01N21/03 ,  G01N35/00 ,  G01N35/04

    Abstract: An apparatus for controlling the temperature of a reaction mixture contained in a reaction vessel, wherein the vessel includes a reaction chamber and at least one port for adding fluid to the chamber, and wherein the chamber has at least one flexible wall, comprises a) a thermal surface for contacting the flexible wall; b) a platen for heat sealing the vessel to seal the port closed and increase the pressure in the chamber, wherein the pressure increase in the chamber is sufficient to force the flexible wall to conform to the thermal surface; and c) at least one thermal element for heating or cooling the thermal surface to induce a temperature change within the chamber.

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