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    COMBINATORIAL NUCLEOBASE OLIGOMERS COMPRISING UNIVERSAL BASE ANALOGUES AND METHODS FOR MAKING AND USING SAME
    11.
    发明申请
    COMBINATORIAL NUCLEOBASE OLIGOMERS COMPRISING UNIVERSAL BASE ANALOGUES AND METHODS FOR MAKING AND USING SAME 审中-公开
    包含通用基础模拟物的组合核酸寡聚体及其制备和使用方法

    公开(公告)号：WO2004111072A3

    公开(公告)日：2005-06-30

    申请号：PCT/US2004018800

    申请日：2004-06-10

    Applicant: APPLERA CORP ,  LIVAK KENNETH J ,  MULLAH K BASHAR

    Inventor： LIVAK KENNETH J ,  MULLAH K BASHAR

    IPC: B01J19/00 ,  C07H21/00 ,  C07H21/04 ,  C07K1/04 ,  C07K14/00 ,  C12N15/11 ,  C12P19/34 ,  C12Q1/68 ,  C40B40/06 ,  C40B50/00

    CPC classification number: C12Q1/6811 ,  C07H21/00 ,  C07H21/04 ,  C12Q2525/197 ,  C12Q2525/117

    Abstract: The invention relates to insulating combinatorial nucleobase oligomers that comprise universal base analogs, where the oligomers are formed by the ligation of two or more oligomer "blocks" via a covalent linkage. Universal bases may serve to insulate specifically binding nucleobases from the effects of the covalent linker region joining two oligomer blocks together, so that the universal bases at least partially negate the Tm penalty caused by the covalent linkage, effective to reduce the required minimal length of the oligomer blocks and the combinatorial oligomer. The resulting insulating nucleobase combinatorial oligomers find use in any hybridization-based application, including use as probes and primers. The combinatorial oligomers of the present invention provide advantages over existing combinatorial oligomer systems currently known in the art.

    Abstract translation: 本发明涉及包含通用碱基类似物的绝缘组合核碱基寡聚体，其中通过共价键连接两个或多个低聚物“嵌段”形成低聚物。 通用碱基可以用于将特异性结合的核碱基与连接两个低聚物嵌段的共价连接体区域的作用绝缘，使得通用碱基至少部分地消除由共价键引起的Tm损失，有效地减少所需的最小长度 低聚物嵌段和组合低聚物。 所得到的绝缘核碱基组合寡聚体可用于任何基于杂交的应用，包括用作探针和引物。 本发明的组合寡聚体提供优于现有技术中已知的组合式低聚物体系的优点。

    SINGLE-CELL TRANSCRIPT SEQUENCING
    15.
    发明专利
    SINGLE-CELL TRANSCRIPT SEQUENCING 未知

    公开(公告)号：CA3027423A1

    公开(公告)日：2018-01-18

    申请号：CA3027423

    申请日：2017-07-12

    Applicant: FLUIDIGM CORP

    Inventor： LIVAK KENNETH J ,  FEKETE RICHARD A

    IPC: C12Q1/68

    Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

    MULTIPLEX AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACID VARIATIONS
    18.
    发明专利
    MULTIPLEX AMPLIFICATION FOR THE DETECTION OF NUCLEIC ACID VARIATIONS 未知

    公开(公告)号：CA2786916A1

    公开(公告)日：2011-07-21

    申请号：CA2786916

    申请日：2011-01-14

    Applicant: UNIV BRITISH COLUMBIA ,  FLUIDIGM CORP

    Inventor： LIVAK KENNETH J ,  HEYRIES KEVIN ,  HANSEN CARL L G ,  PETRIV OLEH

    IPC: C12Q1/68 ,  C12P19/34 ,  C40B30/04 ,  G01N33/50

    Abstract: Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.

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