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    BETA-GALACTOSYL-UMBELLIFERONE-LABELED PROTEIN AND POLYPEPTIDE CONJUGATES
    12.
    发明专利
    BETA-GALACTOSYL-UMBELLIFERONE-LABELED PROTEIN AND POLYPEPTIDE CONJUGATES 未知

    公开(公告)号：CA1155838A

    公开(公告)日：1983-10-25

    申请号：CA414642

    申请日：1982-11-01

    Applicant: MILES LAB

    Inventor： CARRICO ROBERT J ,  NGO THAT T

    IPC: C07H15/26

    Abstract: A .beta.-galactosyl-umbelliferone-labeled conjugate of the formula: wherein -(NH)L is a protein or polypeptide, such as an immunoglobulin, bound through an amino group thereof, n is an integer from 2 through 10, m is an integer from 1 through 10, and p is on the average from 1 to the number of available amino groups in L. An intermediate in the synthesis of such labeled conjugate is also provided. The labeled conjugates are useful as reagents in specific binding assays (e.g., immunoassays) for determining the conjugated protein or polypeptide, or a specific binding analog or partner thereof, in liquid media.

    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME-CLEAVABLE SUBSTRATE AS LABEL
    13.
    发明专利
    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME-CLEAVABLE SUBSTRATE AS LABEL 未知

    公开(公告)号：CA1148080A

    公开(公告)日：1983-06-14

    申请号：CA396228

    申请日：1982-02-12

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  BURD JOHN F

    IPC: C12Q1/34 ,  G01N33/54

    Abstract: An improved homogeneous specific binding assay method for determining a ligand in a liquid medium, wherein a reaction mixture is formed by combining the liquid medium with reagent means, including a conjugate having a label component and a binding component, to form a binding reaction system having a bound-species and a free-species of the labeled conjugate, the label component of the conjugate comprising an enzyme substrateactive portion and an indicator portion whereby the conjugate is cleavable by an enzyme to produce a detectable indicator product, the labeled conjugate being substantially inactive as a substrate for the enzyme when in the bound-species, and wherein the cleaving enzyme is added to the reaction mixture and the resulting indicator product measured as a function of the amount of the ligand in said liquid medium. The improvement comprises employing as the label component of the conjugate, a residue having the formula: wherein R is a linking group through which the residue is covalently bound to the binding component, and employing .beta.-galactosidase as the cleaving enzyme whereby the .beta.-galactosyl group in the residue can be cleaved to release a detectable dye product.

    HOMOGENOUS SPECIFIC BINDING ASSAY METHOD AND COMPOSITION AND DEVICE FOR USE THEREIN
    15.
    发明专利
    HOMOGENOUS SPECIFIC BINDING ASSAY METHOD AND COMPOSITION AND DEVICE FOR USE THEREIN 未知

    公开(公告)号：CA1082577A

    公开(公告)日：1980-07-29

    申请号：CA249745

    申请日：1976-04-07

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  CHRISTNER JAMES E

    IPC: G01N33/542 ,  C07D209/48 ,  C07D237/32 ,  C07D493/10 ,  C07D495/04 ,  C07H19/20 ,  C07H19/207 ,  C07H21/00 ,  C12Q1/00 ,  G01N31/00 ,  G01N33/50 ,  G01N33/532 ,  G01N33/533 ,  G01N33/536 ,  G01N33/543 ,  G01N33/60 ,  G01N33/16

    Abstract: OE OF DISCLOSURE A test composition, device, and method for their use in a homogenous specific binding assay which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium The test composition and device comprise a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.

    DETECTION OF BACTERIA BY NUCLEIC ACID HYBRIDIZATION
    16.
    发明专利
    DETECTION OF BACTERIA BY NUCLEIC ACID HYBRIDIZATION 未知

    公开(公告)号：CA1231303A

    公开(公告)日：1988-01-12

    申请号：CA459077

    申请日：1984-07-17

    Applicant: MILES LAB

    Inventor： CARRICO ROBERT J ,  DERIEMER LESLIE H ,  GROSCH JOSEPHINE C ,  WILSON GARY A

    IPC: G01N33/50 ,  C12Q1/68 ,  G01N33/58

    Abstract: A nucleic acid hybridization method for detecting bacteria in a test sample, such as a mammalian body fluid, employing a polynucleotide probe having a base sequence which is homologous with at least one base sequence in the nucleic acid content or substantially all of the respective bacteria suspected to be present in the sample. The homologous base sequence in the probe preferably comprises at least a 10 base portion of one of the strands of a gene which codes for the synthesis of a nucleic acid or protein comprised in the mechanism of protein synthesis, e.g., tRNAs, rRNAs, and elongation factors. Resulting hybridization between the probe and single stranded nucleic acids released from bacteria in the sample indicates bacterial presence and can be detected by employing labeled probes or otherwise detecting duplex formation. The method provides a unique, direct and rapid means for detecting the presence of bacteria without the need for conventional microbiological culturing. In particular, the method can be applied conveniently as a screening test for bacterial presence in fluids which are normally sterile, such as urine or blood.

    FENCLOFENAC AS TBP BLOCKING AGENT IN IODOTHYRONINE IMMUNOASSAYS
    17.
    发明专利
    FENCLOFENAC AS TBP BLOCKING AGENT IN IODOTHYRONINE IMMUNOASSAYS 未知

    公开(公告)号：DE3273222D1

    公开(公告)日：1986-10-16

    申请号：DE3273222

    申请日：1982-10-25

    Applicant: MILES LAB

    Inventor： ATKINSON DAVID C ,  CARRICO ROBERT J ,  MORRIS DAVID L

    IPC: G01N33/78

    Abstract: An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or piasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.

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