公开(公告)号：EP1463796A4

公开(公告)日：2006-03-08

申请号：EP02786834

申请日：2002-11-27

Applicant: FLUIDIGM CORP

Inventor： UNGER MARC A ,  MANGER IAN DAVID ,  LUCERO MICHAEL ,  YI YONG ,  MIYASHITA-LIN EMILY ,  WIENECKE ANJA ,  FACER GEOFFREY

IPC: G01N33/53 ,  B01F13/00 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/25 ,  C12Q1/68 ,  C40B40/02 ,  C40B50/06 ,  C40B60/12 ,  C40B60/14 ,  F04B43/02 ,  G01N1/00 ,  G01N1/28 ,  G01N27/447 ,  G01N35/00 ,  G01N37/00 ,  C12M1/36 ,  C12N11/16 ,  C12P19/34 ,  G01N15/06

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经被预先沉积。 一旦将样品引入反应部位，试剂就会停止。 这些装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：EP1336097A4

公开(公告)日：2006-02-01

申请号：EP01979990

申请日：2001-10-12

Applicant: FLUIDIGM CORP

Inventor： MANGER IAN DAVID ,  HAO CUNSHENG CASEY ,  UNGER MARC ALEXANDER

IPC: B01L3/00 ,  A61L15/38 ,  A61L15/40 ,  B01L3/02 ,  B81B1/00 ,  B81C1/00 ,  G01N30/72 ,  H01J49/04

CPC classification number: G01N1/10 ,  B01L3/0241 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0481 ,  G01N30/7266 ,  H01J49/0018 ,  H01J49/165 ,  Y10T436/117497 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: This invention provides a microfluidic sample injection apparatus (14, 100) for injecting a fluid sample into an analytical device (120) and a method for using the same. The microfluidic sample injection apparatus (14, 100) comprises a microfluidic device (100) and an integrated sample injection capillary (14) which is in fluid communication with a fluid flow channel of the microfluidic device (100).

Abstract translation: 本发明提供微流体装置及其使用方法。 本发明的微流体装置包括第一弹性层，弹性层内的流体流动通道; 以及用于将流体样品从流体流动通道提供到分析装置的装置。 本发明还提供了一种包括这种微流体装置和分析装置的分析装置。

公开(公告)号：EP3140428A4

公开(公告)日：2018-02-28

申请号：EP15789313

申请日：2015-05-08

Applicant: FLUIDIGM CORP

Inventor： WEST JASON A A ,  FOWLER BRIAN ,  CHARN TZE-HOWE ,  JOHNSON CHRISTIAN F ,  UNGER MARC A

IPC: C12Q1/68

CPC classification number: C12Q1/6855 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q2565/501 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2537/162 ,  C12Q2565/543 ,  C12Q2535/122

Abstract: This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device.

公开(公告)号：EP2971121A4

公开(公告)日：2016-11-30

申请号：EP14763446

申请日：2014-03-14

Applicant: FLUIDIGM CORP

Inventor： JONES ROBERT C

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/682 ,  G01N33/532 ,  G01N2458/10 ,  C12Q2521/101 ,  C12Q2531/113 ,  C12Q2537/125 ,  C12Q2545/114

Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. The methods employ proximity extension assays for detection of a target nucleic acids of interest, e.g., a target RNA. The method can additionally be used in multiplex assays with a protein proximity extension assay to detect protein.

公开(公告)号：EP1796824A4

公开(公告)日：2016-10-19

申请号：EP05766055

申请日：2005-05-02

Applicant: FLUIDIGM CORP

Inventor： GOODSAID FEDERICO ,  UNGER MARC A ,  HUANG JIANG ,  QUAN EMERSON ,  GROSSMAN ROBERT ,  LAM PHILLIP ,  CHOU HOU-PU ,  KIMBALL JAKE ,  PIEPRZYK MARTIN ,  FACER GEOFFREY

IPC: G01N21/00 ,  B01J3/00 ,  B01J4/00 ,  B01J10/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  G01N15/06 ,  G01N31/22

CPC classification number: B01L7/52 ,  B01J19/0046 ,  B01J2219/00353 ,  B01J2219/004 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00576 ,  B01J2219/00587 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0638

公开(公告)号：EP2569447A4

公开(公告)日：2013-11-27

申请号：EP11780930

申请日：2011-05-16

Applicant: FLUIDIGM CORP ,  PIEPRZYK MARTIN

Inventor： PIEPRZYK MARTIN ,  JONES ROBERT C ,  LIVAK KENNETH J ,  MAY ANDREW ,  MIR ALAIN ,  QIN JIAN ,  RAMAKRISHNAN RAMESH ,  SPURGEON SANDRA ,  WANG JUN ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  G01N33/52 ,  G06F19/18

CPC classification number: C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6893 ,  G01N2800/368 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2537/16 ,  C12Q2563/173

公开(公告)号：EP2376227A4

公开(公告)日：2012-07-18

申请号：EP09836688

申请日：2009-12-07

Applicant: FLUIDIGM CORP

Inventor： FOWLER BRIAN

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00398 ,  B01J2219/00418 ,  B01L7/525 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2300/1805 ,  B01L2400/0427

公开(公告)号：EP1730489A4

公开(公告)日：2012-03-07

申请号：EP05712041

申请日：2005-01-25

Applicant: FLUIDIGM CORP

Inventor： GROSSMAN ROBERT ,  UNGER MARC ,  LAM PHILLIP ,  CHOU HOU-PU ,  KIMBALL JAKE ,  PIEPRZYK MARTIN

IPC: G01N15/06 ,  B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  B01L9/00 ,  B01L99/00 ,  C30B7/00 ,  C30B29/58 ,  G01N33/00 ,  G01N33/48

CPC classification number: C12Q1/68 ,  B01L3/0293 ,  B01L3/06 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/5635 ,  B01L3/565 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2400/0487 ,  B01L2400/0655 ,  C30B7/00 ,  C30B29/58 ,  Y10T436/11

Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

公开(公告)号：EP1499706A4

公开(公告)日：2010-11-03

申请号：EP03721509

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： CHOU HOU-PU ,  DARIDON ANTOINE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  JAVADI SHERVIN ,  LIAU YISH-HANN ,  MANGER IAN D ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  THRONDSET WILLIAM

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

CPC classification number: C12M1/34 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0655 ,  C12M21/06 ,  C12M23/16 ,  G01N15/1484 ,  G01N33/4833 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2015/1493 ,  G02B21/32

Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system. Accordingly, these combinations may allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others, as single particles, mixed groups of particles, arrays of particles, heterogeneous particle sets, and/or homogeneous particle sets, among others, in series and/or in parallel. In addition, these combinations may enable microfluidic systems to be reused. Furthermore, these combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

公开(公告)号：EP2970849A4

公开(公告)日：2016-11-02

申请号：EP14763560

申请日：2014-03-14

Applicant: FLUIDIGM CORP

Inventor： WEST JASON A A ,  FOWLER BRIAN

IPC: C12M1/22 ,  G01N33/50 ,  G01N33/569

CPC classification number: G01N35/08 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/10 ,  B01L2300/12 ,  B01L2400/0487 ,  C12M23/24 ,  C12M29/04 ,  G01N15/1056 ,  G01N15/1484 ,  G01N33/5005 ,  G01N33/5097 ,  G01N33/56961 ,  G01N33/56966 ,  G01N2015/1081

Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.