公开(公告)号：WO2003085379A3

公开(公告)日：2003-10-16

申请号：PCT/US2003/009997

申请日：2003-04-01

Applicant: FLUIDIGM CORPORATION ,  CHOU, Hou-Pu ,  DARIDON, Antoine ,  FARRELL, Kevin ,  FOWLER, Brian ,  HAO, Cunsheng (Casey) ,  JAVADI, Shervin ,  LIAU, Yish-Hann ,  MANGER, Ian, D. ,  NASSEF, Hany, Ramez ,  NORTON, Pierce ,  THRONDSET, William

Inventor： CHOU, Hou-Pu ,  DARIDON, Antoine ,  FARRELL, Kevin ,  FOWLER, Brian ,  HAO, Cunsheng (Casey) ,  JAVADI, Shervin ,  LIAU, Yish-Hann ,  MANGER, Ian, D. ,  NASSEF, Hany, Ramez ,  NORTON, Pierce ,  THRONDSET, William

IPC: C12M1/34

Abstract: The invention provides systems (2000), including microfluidic mechanisms, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any number of suitable times in the system to allow particles to be sorted, cultured, mixed, treated, and/or assayed, among others. These combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.

公开(公告)号：WO2003048295A1

公开(公告)日：2003-06-12

申请号：PCT/US2002/038363

申请日：2002-11-27

Applicant: FLUIDIGM CORPORATION ,  UNGER, Marc, A. ,  MANGER, Ian, David ,  LUCERO, Michael ,  YI, Yong ,  MIYASHITA-LIN, Emily ,  WIENECKE, Anja ,  FACER, Geoffrey

Inventor： UNGER, Marc, A. ,  MANGER, Ian, David ,  LUCERO, Michael ,  YI, Yong ,  MIYASHITA-LIN, Emily ,  WIENECKE, Anja ,  FACER, Geoffrey

IPC: C12M1/36

CPC classification number: C12M1/38 ,  B01F13/0059 ,  B01J2219/00337 ,  B01J2219/00353 ,  B01J2219/00403 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/0072 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/123 ,  B01L2400/06 ,  C12Q1/68 ,  C12Q1/6844 ,  C40B60/14 ,  F16K11/20 ,  G01N35/00029 ,  G01N35/0098 ,  G01N2035/00366 ,  Y10T137/2076 ,  Y10T137/2496 ,  Y10T137/86292 ,  Y10T137/87249 ,  Y10T436/2575 ,  C12Q2565/501

Abstract: A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Abstract translation: 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。

公开(公告)号：WO2002060582A3

公开(公告)日：2002-08-08

申请号：PCT/US2001/045256

申请日：2001-11-16

Applicant: FLUIDIGM CORPORATION ,  CHOU, Hou-Pu ,  MANGER, Ian, David ,  UNGER, Marc, A. ,  YI, Yong ,  FERNANDEZ, Dave

Inventor： CHOU, Hou-Pu ,  MANGER, Ian, David ,  UNGER, Marc, A. ,  YI, Yong ,  FERNANDEZ, Dave

IPC: B01L3/02

Abstract: The present invention provides microfluidic devices, systems and methods for using the same, which facilitate the introduction of fluid to and from a microfluidic channel located within the microfluidic devices.

公开(公告)号：WO2021168439A1

公开(公告)日：2021-08-26

申请号：PCT/US2021/019101

申请日：2021-02-22

Applicant: FLUIDIGM CORPORATION

Inventor： PHELAN, Michael L. ,  KUBU, Christopher J. ,  FOWLER, Brian ,  SUN, Gang ,  PATEL, Nikita ,  RAMALINGAM, Naveen

IPC: C12P19/34 ,  B01F11/00 ,  B01F11/02

Abstract: Described herein are methods, kits and systems for sample enrichment, multi-step library preparation, sample normalization, detection of sample biomolecules and combinations thereof. Enrichment and multi-step library preparation is described in the context of microfluidic workflows. Sample barcoding methods and kits are described for increasing sample throughput while reducing background in negative samples. Integrated microfluidic devices comprising sample processing unit cells coupled to an array of reaction sites are provided for integrated workflows.

公开(公告)号：WO2020150236A1

公开(公告)日：2020-07-23

申请号：PCT/US2020/013509

申请日：2020-01-14

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： LOBODA, Alexander ,  SANDKUIJL, Daaf ,  CAREW, Adam

IPC: C12Q1/6872 ,  G01N27/62 ,  G01N33/48 ,  H01J49/00

Abstract: As described herein, one or more parameters of a direct ionization imaging mass spectrometer (IMS) may be set to obtain a desired plasma and deliver it to a mass detector. Depending on the application, certain parameters may be predetermined (e.g., a spot size given a desired resolution) and, as described herein, other parameters can be adjusted to obtain the desired plasma properties. Also included is sample preparation suitable for direct ionization IMS and/or other imaging modalities.

公开(公告)号：WO2020092236A1

公开(公告)日：2020-05-07

申请号：PCT/US2019/058342

申请日：2019-10-28

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： LOBODA, Alexander ,  CAREW, Adam

IPC: H01J49/04 ,  G01N1/28 ,  G01N15/10 ,  H01J49/10 ,  G01N21/68 ,  G01N21/73 ,  H01J49/105 ,  H05H1/30

Abstract: We describe in this application the analysis of samples using elemental or mass spectrometry and the analysis of samples, such as biological samples by suspension mass cytometry or imaging mass cytometry and an inductively coupled plasma torch with reverse vortex flow for elemental analysis and a method of operating an ICP torch configured to interface with a spectrometer.

公开(公告)号：WO2020055813A1

公开(公告)日：2020-03-19

申请号：PCT/US2019/050333

申请日：2019-09-10

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： SANDKUIJL, Daaf ,  LOBODA, Alexander ,  CAREW, Adam ,  LAKSHMAN, Minalini

IPC: H01J49/04 ,  G01N1/28 ,  H01J49/02 ,  H01J49/06 ,  H01J49/26

Abstract: This disclosure relates to systems and methods for high speed modulation sample imaging. Disclosed herein are systems and methods for performing imaging mass cytometry, including analysis of labelling atoms by elemental (e.g., atomic) mass spectrometry. Aspects include a sampling system having, and method of using, a femtosecond (fs) laser and/or laser scanning. Alternatively or in addition, aspects include systems and methods for co-registering other imaging modalities with imaging mass cytometry.

公开(公告)号：WO2020055810A1

公开(公告)日：2020-03-19

申请号：PCT/US2019/050330

申请日：2019-09-10

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： SANDKUIJL, Daaf ,  LOBODA, Alexander ,  CAREW, Adam ,  ASKARPOUR, Khashayar

IPC: G01N1/04 ,  G02B21/00 ,  H01J49/04 ,  H01J49/10

Abstract: The invention relates to systems and methods for autofocusing in imaging mass spectrometry. The present invention provides improvements over current IMS and IMG apparatus and methods through an autofocus component including plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so reducing the number of unsuccessful autofocus attempts.

公开(公告)号：WO2019200307A1

公开(公告)日：2019-10-17

申请号：PCT/US2019/027306

申请日：2019-04-12

Applicant: FLUIDIGM CANADA INC. ,  FLUIDIGM CORPORATION

Inventor： BARANOV, Vladimir ,  LOBODA, Alexander ,  SULLIVAN, Michael

IPC: G01N33/50 ,  G01N33/532 ,  G01N33/543 ,  G01N33/544 ,  G01N33/566 ,  G01N33/58 ,  G01N30/72

Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.

公开(公告)号：WO2019036723A1

公开(公告)日：2019-02-21

申请号：PCT/US2018/047095

申请日：2018-08-20

Applicant: FLUIDIGM CORPORATION

Inventor： QIN, Jian ,  CHEN, Peilin ,  RAMAKRISHNAN, Ramesh

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Disclosed herein is a method and kit thereof for isolating nucleic acids from wax-embedded samples using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids using ethanol, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.