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公开(公告)号：KR1020030062126A
公开(公告)日：2003-07-23
申请号：KR1020020002561
申请日：2002-01-16
Applicant: (주)케비젠
IPC: C12N15/53
CPC classification number: C12N9/0069 , C12P7/02 , C12Y113/11063
Abstract: PURPOSE: Mutant genes of human beta, beta-carotene 15, 15'-dioxygenase having improved enzyme activity are provided, so that enzyme proteins expressed from the mutant genes can be used in various fields related to the vitamin A production. CONSTITUTION: Mutant genes of human beta, beta-carotene 15, 15'-dioxygenase are produced by error-prone PCR using a human beta, beta-carotene 15, 15'-dioxygenase gene as templates, and have the enzyme activity of the expressed protein of 1100 pmol/h. A method for producing vitamin A comprises digesting beta-carotene using the expressed human beta, beta-carotene 15, 15'-dioxygenase. A method for producing vitamin A comprises transforming a host cell using the mutant genes of human beta, beta-carotene 15, 15'-dioxygenase, and culturing the transformed host cell in a medium, wherein the host cell is a plant cell, a prokaryotic cell, a yeast cell, an insect cell, a mammal cell or a poultry cell.
Abstract translation: 目的：提供具有改善的酶活性的人β，β-胡萝卜素15,15'-双加氧酶的突变基因，使得从突变基因表达的酶蛋白可用于与维生素A生产相关的各种领域。 构成：使用人β，β-胡萝卜素15,15'-双加氧酶基因作为模板，通过易错PCR产生人β，β-胡萝卜素15,15'-双加氧酶的突变基因，并具有表达的酶活性 蛋白质为1100 pmol / h。 生产维生素A的方法包括使用表达的人β，β-胡萝卜素15,15'-双加氧酶消化β-胡萝卜素。 生产维生素A的方法包括使用人β，β-胡萝卜素15,15'-双加氧酶的突变基因转化宿主细胞，并在培养基中培养转化的宿主细胞，其中宿主细胞是植物细胞，原核 细胞，酵母细胞，昆虫细胞，哺乳动物细胞或家禽细胞。
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22.发明授权
公开(公告)号：KR100758664B1
公开(公告)日：2007-09-13
申请号：KR1020060076966
申请日：2006-08-16
Applicant: (주)케비젠
IPC: B01J13/02
CPC classification number: B01J13/02
Abstract: A method of preparing a microcapsule having improved dispersibility and stability as compared with conventional technologies of capsules containing an unsaturated fatty acid, the microcapsule prepared by the method, and articles comprising the microcapsule are provided. A method of preparing a microcapsule containing an unsaturated fatty acid comprises the steps of: (a) mixing and gelatinizing polyglycerin fatty acid ester and one or more gums consisting of xanthan gum, guar gum and locust bean gum in sterilized water, adding an unsaturated fatty acid into the mixed and gelatinized solution, and homogenizing the mixed solution, thereby preparing a first coating material; (b) mixing and gelatinizing starch or modified starch, gelatin or casein, and polyglycerin fatty acid ester in sterilized water, injecting the first coating material into the mixed and gelatinized solution, and homogenizing the mixed solution, thereby preparing a second coating material; and (c) spraying the second coating material in sterilized cooling water. The unsaturated fatty acid is arachidonic acid, DHA(docosahexaenoic acid), EPA(Eicosapentaenoic acid), DPA(Docosapentaenoic acid), or CLA(conjugated linolenic acid).
Abstract translation: 提供了与含有不饱和脂肪酸的胶囊，通过该方法制备的微胶囊和包含微胶囊的制品的常规技术相比，具有改进的分散性和稳定性的微胶囊的制备方法。 一种制备含有不饱和脂肪酸的微胶囊的方法包括以下步骤：（a）将聚甘油脂肪酸酯和一种或多种由黄原胶，瓜尔胶和刺槐豆胶组成的胶在无菌水中混合和胶凝，加入不饱和脂肪 酸混合并糊化溶液，并均化混合溶液，从而制备第一涂料; （b）将淀粉或改性淀粉，明胶或酪蛋白和聚甘油脂肪酸酯在无菌水中混合和凝胶化，将第一涂料注入混合糊化溶液中，均化混合溶液，从而制备第二涂料; 和（c）将第二涂层材料喷洒到灭菌的冷却水中。 不饱和脂肪酸是花生四烯酸，DHA（二十二碳六烯酸），EPA（二十碳五烯酸），DPA（Docosapentaenoic acid）或CLA（共轭亚麻酸））。
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公开(公告)号：KR100654959B1
公开(公告)日：2006-12-06
申请号：KR1020050026100
申请日：2005-03-29
Abstract: 본 발명은 산수유, 구기자, 복분자, 해송자, 진주가루 추출물을 함유하는 미백용 화장료 조성물을 제공한다.
본 발명의 미백용 화장료 조성물은 티로시나제 활성을 억제하는 효과가 있고, 멜라닌 생성을 억제하는 효과가 있으며, 세포독성이 낮아 피부에 자극이 없어 안전하므로 피부 미백용 화장품에 유용하게 사용될 수 있다.-
公开(公告)号：KR100516377B1
公开(公告)日：2005-09-26
申请号：KR1020030023164
申请日：2003-04-12
Applicant: (주)케비젠
IPC: C12N1/20
CPC classification number: C12P7/6427 , A23C9/1234 , A23C11/106 , A23L33/12 , A23L33/135 , A23P10/30 , A23Y2300/59 , A61K35/744 , A61K35/745 , C12R1/13
Abstract: 본 발명은 공역화 리놀레인산(CLA)을 생산할 수 있는 신규한 균주에 관한 것이다.
본 발명의 균주에는 비피도박테리움 브레베(
Bifidobacterium breve ) CBG-C2 균주, 비피도박테리움 슈도카르테눌라툼(
Bifidobacterium pseudocartenulatum ) CBG-C4 균주 및 엔테로코커스 패시움(
Enterococcus faecium ) CBG-C5 균주가 포함된다.
본 발명의 균주는 CLA 생산 능력이 우수하며, CLA를 생성하여 배지로 분비하는 한편 균체 내에 축적할 수 있다. 또한 본 발명의 균주는 위산이나 담즙 등의 산과 항생물질에 대해 강한 내성을 지닌다.
본 발명의 균주를 포함하는 조성물은 수용성 다당류로 이루어진 피복물질 내에 본 발명의 균주와 CLA를 포함하는 캡슐제 형태로 제조되어, 기능성 식품 및 의약품으로 사용될 수 있다.-
公开(公告)号：KR100464061B1
公开(公告)日：2004-12-30
申请号：KR1020020026615
申请日：2002-05-15
Applicant: (주)케비젠
IPC: C12P19/02
Abstract: PURPOSE: A method for producing tagatose by immobilization of arabinose isomerase is provided. Tagatose is cheaply and effectively mass-produced by maintaining the enzyme activity for a long time. CONSTITUTION: A method for producing tagatose by immobilization of arabinose isomerase comprises binding arabinose isomerase to resin, bridging arabinose isomerase with glutaraldehyde to immobilize the arabinose isomerase, and reacting the immobilized arabinose isomerase with substrate, wherein the concentration of arabinose isomerase is 10 mg/ml; the resin is sodium alginate, alumina or silica; the sodium alginate is used by dissolving 1.5 to 4.0% of sodium alginate in water; the alumina is used by dissolving 60 to 70% of alumina in 50 mM tris buffer solution; the silica is used by dissolving 60 to 70% of silica in 50 mM tris buffer solution; substrate is galactose; the galactose is used by dissolving 45 to 55% of galactose in pH 7 to 8 tris buffer solution; and the reaction temperature is 55 to 65 deg. C.
Abstract translation: 目的：提供一种通过固定阿拉伯糖异构酶来生产塔格糖的方法。 塔格糖通过长期维持酶活性而廉价且有效地大量生产。 构成：通过固定阿拉伯糖异构酶生产塔格糖的方法包括将阿拉伯糖异构酶与树脂结合，将阿拉伯糖异构酶与戊二醛桥接以固定阿拉伯糖异构酶，并使固定的阿拉伯糖异构酶与底物反应，其中阿拉伯糖异构酶的浓度为10mg / ml ; 该树脂是海藻酸钠，氧化铝或二氧化硅; 海藻酸钠通过在水中溶解1.5至4.0％的海藻酸钠来使用; 通过在50mM tris缓冲溶液中溶解60-70％的氧化铝来使用氧化铝; 二氧化硅是通过将60-70％的二氧化硅溶解在50mM tris缓冲溶液中而使用的; 底物是半乳糖; 半乳糖通过在pH 7至8 Tris缓冲溶液中溶解45-55％半乳糖来使用; 反应温度为55〜65℃。 C。
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Patent Agency Ranking
公开(公告)号：KR100437102B1
公开(公告)日：2004-06-23
申请号：KR1020020001178
申请日：2002-01-09
Applicant: (주)케비젠
IPC: C07K5/00
Abstract: PURPOSE: Provided are a novel retinol derivative, its preparation method in higher yield, and its use. The novel retinol has excellent light-stability, and shows high reactivity to retinoic acid receptor α, while showing low reactivity to retinoic receptor β and γ. It can applied to medical products, cosmetics, soap, shampoo, functional foods, etc., for the prevention and improvement of skin aging. CONSTITUTION: The novel retinol derivative is characterized by carboester bond of a peptide material having COOH group, wherein the peptide material having COOH group is selected from -di, -tri, -poly peptide including N-L- α-aspartyl-L-phenylalanine 1-methylester(AMP;aspartame), N-protection group-aspartame, neotame and the like. Its manufacturing method comprises the steps of: reacting retinylacetate with methanolic solvent and inorganic slat at 25-40 deg.C in a dark room then extracting the reaction product with ether solvent; removing the solvent then followed by mixing a compound having OH group, natural or separated and purified retinol, diethylazodicarboxylate and triphenylphosphate with methylenechloride solvent, and reacting them at room temperature to obtain the ester derivative of retinol; and performing chromatography with reverse-phase, Merck Silicagel 60 RP 18(40-63) micro meter to separate pure ester derivative of retinol.
Abstract translation: 目的：提供一种新颖的视黄醇衍生物及其制备方法，其收率较高，并且其用途。 该新型视黄醇具有优异的光稳定性，并且对视黄酸受体α具有高反应性，而对视黄酸受体β具有低反应性; 和＆amp; gamma ;. 它可以应用于医疗产品，化妆品，肥皂，洗发水，功能性食品等，以预防和改善皮肤老化。 构成：新颖的视黄醇衍生物的特征在于具有COOH基团的肽材料的羧基酯键，其中具有COOH基团的肽材料选自包括NL-和α-天冬氨酰-L- 苯丙氨酸1-甲酯（AMP;天冬甜素），N-保护基 - 阿斯巴甜，纽甜等。 其制造方法包括以下步骤：在25-40℃，在黑暗的房间内将乙酸视黄醇酯与甲醇溶剂和无机薄片反应，然后用乙醚溶剂萃取反应产物; 然后除去溶剂，然后将具有OH基团的化合物，天然或分离纯化的视黄醇，偶氮二羧酸二乙酯和磷酸三苯酯与二氯甲烷溶剂混合，并在室温下反应，得到视黄醇的酯衍生物; 并用反相色谱（Merck Silicagel 60 RP 18（40-63）微米）进行色谱分离以分离视黄醇的纯酯衍生物。
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公开(公告)号：KR1020040020426A
公开(公告)日：2004-03-09
申请号：KR1020020052026
申请日：2002-08-30
Applicant: (주)케비젠
IPC: C07C403/20
Abstract: PURPOSE: Retinoid derivatives in cis form having substantially different chemical structure while having increased antitumor effects but reduced negative side effects compared with the conventional retinoid compounds, are provided. CONSTITUTION: The retinoid derivatives are represented by formula (I) and can be in cis form especially at the position of 9 and/or 13, wherein X is O, NH or S; R1 and R2 are the same or different, and independently -OH, -SH, -NH2, -COOH, -R(CH2)mCH3, -RCOCO(CH2)mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)m(CH)3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2(CH2)mCH4, -RPO2(OH)CH2(CH2)mCH3 or -RCOCH(NHCOCH3)CH2CH2CONH2; and R3 is H, wherein R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and m is an integer of 0 to 5.
Abstract translation: 目的：提供具有与常规类视黄醇化合物相比具有显着不同化学结构同时具有增加的抗肿瘤作用但降低的副作用的顺式形式的类视黄醇衍生物。 构成：类视黄醇衍生物由式（I）表示，并且可以是顺式，特别是在9和/或13位，其中X是O，NH或S; R1和R2相同或不同，独立地是-OH，-SH，-NH2，-COOH，-R（CH2）mCH3，-RCOCO（CH2）mCH3，-RCO（CH2）mCHCH3CH3，-RCO（CH2）mNR4CH3 ，-RCOCHOH（CH2）mCH3，-RCOCH2（CH2）m（CH）3，-RCOCH2CHOH（CH2）mCH3，-RCOCH2（CH2）mCOOH，-RSO2CH2（CH2）mCH4，-RPO2（OH）CH2（CH2） 或-RCOCH（NHCOCH 3）CH 2 CH 2 CONH 2; 并且R 3是H，其中R是CH 2，O，NH或S，R 4是H或C 1 -C 6烷基，m是0-5的整数。
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公开(公告)号：KR1020030088749A
公开(公告)日：2003-11-20
申请号：KR1020020026615
申请日：2002-05-15
Applicant: (주)케비젠
IPC: C12P19/02
Abstract: PURPOSE: A method for producing tagatose by immobilization of arabinose isomerase is provided. Tagatose is cheaply and effectively mass-produced by maintaining the enzyme activity for a long time. CONSTITUTION: A method for producing tagatose by immobilization of arabinose isomerase comprises binding arabinose isomerase to resin, bridging arabinose isomerase with glutaraldehyde to immobilize the arabinose isomerase, and reacting the immobilized arabinose isomerase with substrate, wherein the concentration of arabinose isomerase is 10 mg/ml; the resin is sodium alginate, alumina or silica; the sodium alginate is used by dissolving 1.5 to 4.0% of sodium alginate in water; the alumina is used by dissolving 60 to 70% of alumina in 50 mM tris buffer solution; the silica is used by dissolving 60 to 70% of silica in 50 mM tris buffer solution; substrate is galactose; the galactose is used by dissolving 45 to 55% of galactose in pH 7 to 8 tris buffer solution; and the reaction temperature is 55 to 65 deg. C.
Abstract translation: 目的：提供通过阿拉伯糖异构酶固定生产塔格糖的方法。 通过长时间维持酶活性，塔提糖便宜而有效地批量生产。 构成：通过阿拉伯糖异构酶的固定制备塔格糖的方法包括将阿拉伯糖异构酶与树脂结合，用戊二醛桥接阿拉伯糖异构酶以固定阿拉伯糖异构酶，并使固定的阿拉伯糖异构酶与底物反应，其中阿拉伯糖异构酶的浓度为10mg / ml ; 树脂是藻酸钠，氧化铝或二氧化硅; 通过将1.5〜4.0％的藻酸钠溶于水中使用藻酸钠; 通过将60〜70％的氧化铝溶解在50mM tris缓冲溶液中来使用氧化铝; 通过将60〜70％的二氧化硅溶解在50mM tris缓冲溶液中来使用二氧化硅; 底物是半乳糖; 通过将45至55％的半乳糖溶解在pH7至8 tris缓冲溶液中来使用半乳糖; 反应温度为55〜65℃。 C。
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公开(公告)号：KR1020030075213A
公开(公告)日：2003-09-26
申请号：KR1020020014263
申请日：2002-03-16
Applicant: (주)케비젠
IPC: A61K9/50
CPC classification number: A61K9/7007 , A61K9/5021
Abstract: PURPOSE: A process of preparing a water-soluble film having a high solid content, a low viscosity at wide pH range and excellent dispersion stability without causing solidification is provided. Therefore, the film has excellent dispersion stability and prevents the anticancer substances from being released fast after the destruction of the resin film and is thus effectively used in controlled released drug delivery systems, skin absorbing agents, patch agents or the like. CONSTITUTION: A water-soluble film(1) is prepared by: forming a microcapsule by coating anticancer substances(3) with aminoaldehyde polycondensated resin; and then coating the microcapsule with a water-soluble polymer.
Abstract translation: 目的：提供固体成分高，在宽pH范围内的低粘度，优异的分散稳定性而不引起凝固的水溶性薄膜的制造方法。 因此，该膜具有优异的分散稳定性，并且防止了抗癌物质在树脂膜破坏之后快速释放，因此有效地用于受控释放的药物递送系统，皮肤吸收剂，贴剂等中。 构成：通过以下方法制备水溶性薄膜（1）：通过用氨基醛缩聚树脂涂覆抗癌物质（3）形成微胶囊; 然后用水溶性聚合物涂布微胶囊。
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公开(公告)号：KR1020020060598A
公开(公告)日：2002-07-18
申请号：KR1020020001178
申请日：2002-01-09
Applicant: (주)케비젠
IPC: C07K5/00
CPC classification number: C07C403/08 , A23L33/155 , A61K8/671 , A61K31/07 , A61Q5/02 , A61Q19/005 , A61Q19/008 , A61Q19/10 , C07K5/04 , C07K19/00
Abstract: PURPOSE: Provided are a novel retinol derivative, its preparation method in higher yield, and its use. The novel retinol has excellent light-stability, and shows high reactivity to retinoic acid receptor α, while showing low reactivity to retinoic receptor β and γ. It can applied to medical products, cosmetics, soap, shampoo, functional foods, etc., for the prevention and improvement of skin aging. CONSTITUTION: The novel retinol derivative is characterized by carboester bond of a peptide material having COOH group, wherein the peptide material having COOH group is selected from -di, -tri, -poly peptide including N-L- α-aspartyl-L-phenylalanine 1-methylester(AMP;aspartame), N-protection group-aspartame, neotame and the like. Its manufacturing method comprises the steps of: reacting retinylacetate with methanolic solvent and inorganic slat at 25-40 deg.C in a dark room then extracting the reaction product with ether solvent; removing the solvent then followed by mixing a compound having OH group, natural or separated and purified retinol, diethylazodicarboxylate and triphenylphosphate with methylenechloride solvent, and reacting them at room temperature to obtain the ester derivative of retinol; and performing chromatography with reverse-phase, Merck Silicagel 60 RP 18(40-63) micro meter to separate pure ester derivative of retinol.
Abstract translation: 目的：提供一种新型视黄醇衍生物，其制备方法较高产率及其用途。 该新型视黄醇具有优异的光稳定性，对视黄酸受体α具有高反应性，而对视黄酸受体β和γ的反应性低。 适用于医疗用品，化妆品，肥皂，洗发水，功能性食品等，用于预防和改善皮肤老化。 构成：新型视黄醇衍生物的特征在于具有COOH基团的肽材料的碳键，其中具有COOH基团的肽材料选自-di，-tri， - 多肽，包括NL-α-天冬氨酰-L-苯丙氨酸1- 甲基酯（AMP;阿斯巴甜），N-保护基 - 阿斯巴甜，neotame等。 其制造方法包括以下步骤：将黑醋酸乙烯酯与甲醇溶剂和无机条板在25-40℃下在暗室中反应，然后用乙醚溶剂萃取反应产物; 除去溶剂，然后将具有OH基团的化合物，天然或分离的纯化的视黄醇，偶氮二甲酸二乙酯和磷酸三苯酯与二氯甲烷溶剂混合，并在室温下使其反应，得到视黄醇的酯衍生物; 并用反相色谱法，Merck Silicagel 60 RP 18（40-63）微米分离纯化的视黄醇酯衍生物。
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