公开(公告)号：KR1019980002247A

公开(公告)日：1998-03-30

申请号：KR1019960019826

申请日：1996-06-04

Applicant: 한국과학기술연구원 ,  주식회사농심

Inventor： 오태광 ,  박미자 ,  김형권 ,  배경숙 ,  최인재 ,  남희섭 ,  신재익 ,  이형재

IPC: C12N1/20

公开(公告)号：KR100127100B1

公开(公告)日：1997-12-29

申请号：KR1019940010758

申请日：1994-05-17

Applicant: 한국과학기술연구원

Inventor： 오태광 ,  이정기 ,  김형권 ,  김기현 ,  윤기홍 ,  성문희 ,  김금자 ,  신재익 ,  남희섭

IPC: C12N1/20

Abstract: PSEUDOMONAS SP. NS-83(KCTC 8600P) AND production of novel protein hydrolysis enzyme is disclosed in this invention. PSEUDOMONAS SP. NS-83(KCTC 8600P) is injected in PH6-8 of medium made by polypepton, extract of enzyme, extract of cow meat, glycol, KH2PO4, K2HPO4, MgSO4.7H2O and NaCl, cultivated for 6 hours at 35-40 degree Celsius. Thereafter, above-cultivated liquid is fermented in medium made by aqueous starch, phytonpepton, KH2PO4, K2HPO4, MgSO4.7H2O and NaCl, for 15 hours at 50 degree Celsius to produce protein hydrolysis enzyme having characteristics below; molecular weight : 32,000 dalton, optimum pH : 8.0, optimum temperature : 60deg.C.

Abstract translation: PSEUDOMONAS SP。 NS-83（KCTC 8600P）和新型蛋白质水解酶的生产在本发明中公开。 PSEUDOMONAS SP。 将NS-83（KCTC 8600P）注射到由多肽，酶提取物，牛肉提取物，二醇，KH 2 PO 4，K 2 HPO 4，MgSO 4·7H 2 O和NaCl制成的培养基的PH6-8中，在35-40摄氏度下培养6小时 。 此后，将上述培养液体在含水淀粉，植物磷酸盐，KH 2 PO 4，K 2 HPO 4，MgSO 4·7H 2 O和NaCl的培养基中在50℃下发酵15小时以产生具有以下特征的蛋白质水解酶; 分子量：32,000道尔顿，最适pH：8.0，最适温度：60℃。