신균주 바실러스 속(Bacillus sp.) NS-70(KCTC 8555P)와 이를 이용한 신규한 단백질 가수분해효소의 제조방법
    5.
    发明授权

    公开(公告)号:KR100127099B1

    公开(公告)日:1997-12-29

    申请号:KR1019940011237

    申请日:1994-05-24

    Abstract: The preparation method for the novel Bacillus sp. cell line NS-70 (KCTC 8555P) and the novel protease by using the bacillus sp. NS-70 includes the steps of: adding bacillus sp. cell line NS-70 (KCTC 8555P) to a culture containing polypeptone, yeast extract, beef extract, glycerol, KH2PO4, K2HPO4, MgSO4,7H2O and NaCl at pH 6 to 8 and fermenting the culture placed at 55 degrees centigrade for 6 hours in a culture containing phytonpeptone, K2HPO4, KH2HPO4, MgSO4,7H2O and NaCl at 50 degrees centigrade for 8-10 hours.

    Abstract translation: 新型芽孢杆菌的制备方法 细胞系NS-70(KCTC 8555P)和新型蛋白酶。 NS-70包括以下步骤:添加芽孢杆菌 细胞系NS-70(KCTC 8555P)在含有多胨,酵母提取物,牛肉提取物,甘油,KH 2 PO 4,K 2 HPO 4,MgSO 4·7H 2 O和NaCl的培养基中,pH6〜8,并将培养物放置在55℃下放置6小时 在50摄氏度下含有植物蛋白胨,K 2 HPO 4,KH 2 HPO 4,MgSO 4·7H 2 O和NaCl的培养物8-10小时。

    신균주 슈우도모나스 속(Pseudomonas sp.) NS-83(KCTC 8600P)와 이를 이용한 신규한 단백질 가수분해효소의 제조방법

    公开(公告)号:KR100127100B1

    公开(公告)日:1997-12-29

    申请号:KR1019940010758

    申请日:1994-05-17

    Abstract: PSEUDOMONAS SP. NS-83(KCTC 8600P) AND production of novel protein hydrolysis enzyme is disclosed in this invention. PSEUDOMONAS SP. NS-83(KCTC 8600P) is injected in PH6-8 of medium made by polypepton, extract of enzyme, extract of cow meat, glycol, KH2PO4, K2HPO4, MgSO4.7H2O and NaCl, cultivated for 6 hours at 35-40 degree Celsius. Thereafter, above-cultivated liquid is fermented in medium made by aqueous starch, phytonpepton, KH2PO4, K2HPO4, MgSO4.7H2O and NaCl, for 15 hours at 50 degree Celsius to produce protein hydrolysis enzyme having characteristics below; molecular weight : 32,000 dalton, optimum pH : 8.0, optimum temperature : 60deg.C.

    Abstract translation: PSEUDOMONAS SP。 NS-83(KCTC 8600P)和新型蛋白质水解酶的生产在本发明中公开。 PSEUDOMONAS SP。 将NS-83(KCTC 8600P)注射到由多肽,酶提取物,牛肉提取物,二醇,KH 2 PO 4,K 2 HPO 4,MgSO 4·7H 2 O和NaCl制成的培养基的PH6-8中,在35-40摄氏度下培养6小时 。 此后,将上述培养液体在含水淀粉,植物磷酸盐,KH 2 PO 4,K 2 HPO 4,MgSO 4·7H 2 O和NaCl的培养基中在50℃下发酵15小时以产生具有以下特征的蛋白质水解酶; 分子量:32,000道尔顿,最适pH:8.0,最适温度:60℃。

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