公开(公告)号：KR100755509B1

公开(公告)日：2007-09-04

申请号：KR1020060048321

申请日：2006-05-29

Applicant: 대한민국(농촌진흥청장) ,  충북대학교 산학협력단

Inventor： 이윤정 ,  이상범 ,  유재홍 ,  이상민 ,  김한명 ,  최두회 ,  류갑희 ,  김승환 ,  원항연 ,  사동민 ,  김충우

IPC: C12N1/20 ,  C05F11/08

Abstract: A novel strain Azospirillum brasilense CW301 with nitrogen fixing ability as well as growth enhancing effect is provided to transfer the fixed nitrogen to plants and increase nitrogen absorption of the plants, so that the strain is used as a substitute for chemical fertilizer. A strain Azospirillum brasilense CW301(KACC 91224P) has nitrogen fixing ability as well as growth enhancing effect and is isolated from wheat. The biofertilizer contains Azospirillum brasilense CW301(KACC 91224P) and is prepared by culturing Azospirillum brasilense CW301(KACC 91224P). The growth of plants is promoted by soaking seeds of plants in the cultured medium of Azospirillum brasilense CW301(KACC 91224P), wherein the plant is tomato, Chinese cabbage, lettuce, cucumber, red pepper, Welsh onion or crown daisy.

Abstract translation: 提供了一种具有氮固定能力和生长增强效果的新型菌株Azospirillum brasilense CW301，用于将固定氮转移到植物中并增加植物的氮吸收，从而将该菌株用作化肥的替代品。 Azospirillum brasilense CW301（KACC 91224P）具有固氮能力和生长增强效果，并从小麦中分离出来。 生物肥料含有Azospirillum brasilense CW301（KACC 91224P），并通过培养拟南芥（Azospirillum brasilense）CW301（KACC 91224P）制备。 通过将植物的种子浸泡在巴西青蒿（CWA）（KACC 91224P）的培养基中，其中植物是番茄，大白菜，莴苣，黄瓜，红辣椒，威尔士洋葱或冠状菊花，​​促进植物的生长。

公开(公告)号：KR1020150134004A

公开(公告)日：2015-12-01

申请号：KR1020140060752

申请日：2014-05-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 인트론바이오테크놀로지

Inventor： 이경기 ,  엄재구 ,  김성희 ,  박최규 ,  이윤정 ,  강현미 ,  이은경 ,  송병민 ,  김혜령 ,  최준구 ,  윤성준 ,  최윤혁 ,  박지성 ,  전수연 ,  강상현

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/686

Abstract: 본발명은개선된검출정확성을제공해줄 수있는핵산검사기반의조류인플루엔자바이러스검출및 H5형, H7형, 및 H9형동시감별검출키트에관한것이다. 본발명에따른조류인플루엔자바이러스검출및 H5형, H7형, 및 H9형동시감별검출키트는조류인플루엔자바이러스의목적유전자에서의짧은보존서열로인한문제점을극복할수 있는서열번호 1 내지서열번호 16으로특징지어지는특이프라이머들이적용되고, 또한기존조류인플루엔자바이러스검출용다중유전자증폭에서의민감도저하및 위음성발생문제를회피하기위하여 8개의튜브로구성된다중튜브방식이적용된것을특징으로한다. 본발명의조류인플루엔자바이러스검출및 H5형, H7형및 H9형을동시감별검출키트는조류인플루엔자바이러스검출과임상적으로주요한조류인플루엔자바이러스 H5형, 조류인플루엔자 H7형및 조류인플루엔자 H9형의동시감별검출에있어유용하게활용될수 있다.

Abstract translation: 本发明涉及用于禽流感病毒的试剂盒和基于具有改进的检测精度的核酸测试的H5-，H7-和H9-型同时差异检测。 根据本发明的试剂盒应用特征为SEQ ID NO：1至16的特异性引物，其可以克服由于禽流感病毒的靶基因中短的保守序列引起的问题; 并应用8管形成的多管法，以避免禽流感病毒检测现有多基因扩增中灵敏度降低和假阴性产生的问题。 根据本发明的试剂盒可以有用地用于禽流感病毒检测和H5型禽流感病毒，H7型禽流感病毒和H9型禽流感病毒的同时差异检测，这在临床上是重要的。

公开(公告)号：KR1020130116609A

公开(公告)日：2013-10-24

申请号：KR1020120039157

申请日：2012-04-16

Applicant: 바이오코아 주식회사 ,  건국대학교 산학협력단 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 황승용 ,  김지훈 ,  이원선 ,  송창선 ,  이동훈 ,  이윤정 ,  강현미 ,  최준구 ,  권준헌 ,  박최규

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/701 ,  C12Q1/04 ,  C12Q1/6837 ,  C12Q1/6844

Abstract: PURPOSE: A probe composition for detecting avian influenza virus is provided to simultaneously test the subtypes of HA gene and NA gene and pathogenicity and to quickly and accurately diagnose avian influenza virus. CONSTITUTION: A probe composition for determining serotype and pathogenicity of avian influenza virus contains: at least one probe selected among oligonucleotides with base sequences of sequence numbers 10-24 and complementary oligonucleotides thereof for determining the subtype of HA gene in avian influenza virus; at least one probe selected among oligonucleotides with base sequences of sequence numbers 27-46 and complementary oligonucleotides thereof for determining high pathogenicity of avian influenza virus; and at least one probe selected among oligonucleotides with base sequences of sequence numbers 47-63 and complementary oligonucleotides thereof for determining low pathogenicity of avian influenza virus.

Abstract translation: 目的：提供用于检测禽流感病毒的探针组合物，同时测试HA基因亚型和NA基因的致病性，并迅速准确地诊断禽流感病毒。 构成：用于测定禽流感病毒的血清型和致病性的探针组合物含有：选自具有序列号为10-24的碱基序列的寡核苷酸和其互补寡核苷酸的至少一种探针，用于确定禽流感病毒中HA基因的亚型; 选自具有序列号为27-46的碱基序列的寡核苷酸和其互补寡核苷酸的至少一个探针，用于确定禽流感病毒的高致病性; 和选自具有序列号47-63的碱基序列的寡核苷酸和其互补寡核苷酸中的至少一种探针，用于确定禽流感病毒的低致病性。

公开(公告)号：KR1020090109246A

公开(公告)日：2009-10-20

申请号：KR1020080034591

申请日：2008-04-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 유재홍 ,  이상범 ,  고현관 ,  이윤정 ,  류갑희

IPC: C12N1/20 ,  C12N1/00

Abstract: PURPOSE: A novel Pseudomonas stutzeri. NIST-1 strain with odor reduction effect is provided to relieve problem regarding odor in livestock industry and produce a bio fertilizer. CONSTITUTION: A method for producing a novel Pseudomonas stutzeri. NIST-1 strain with odor reduction comprises: a step of smearing feces of cattle on NB plate medium and culturing at 30°C for one to two days; a step of isolating strain and shaking at 30°C for 76 hours; and a step of screening best strain and isolating.

Abstract translation: 目的：一种新型的Pseudomonas stutzeri。 提供具有气味减少效果的NIST-1菌株，以减轻畜牧业中气味的问题，生产生物肥料。 构成：生产新型Pseudomonas stutzeri的方法。 具有气味减少的NIST-1菌株包括：在NB平板培养基上涂抹牛的粪便并在30℃下培养一至两天的步骤; 在30℃下分离菌株和振荡76小时的步骤; 并筛选最佳应变和分离的步骤。

公开(公告)号：KR100790801B1

公开(公告)日：2008-01-04

申请号：KR1020050060920

申请日：2005-07-06

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 성환우 ,  이윤정 ,  최준구 ,  이은경 ,  권준헌 ,  김재홍

IPC: C12N7/01 ,  C12N7/04

Abstract: 본 발명은 HA 단백질 분절부위 아미노산 배열의 모티프가 TSGR을 가지며, 국내유행 저병원성 조류인플루엔자 바이러스에 방어능력을 나타내는 백신제조용 H9N2 혈청형의 저병원성 조류인플루엔자 바이러스주를 제공한다. 상기 바이러스주를 백신제조용 원료로 사용하는 경우 양계산업에 있어 산란율 저하 및 폐사 등 지속적인 문제를 일으키는 H9N2 혈청형 저병원성 조류인플루엔자 예방에 활용이 가능하다.
저병원성 조류인플루엔자, H9N2, 백신

公开(公告)号：KR1020070121468A

公开(公告)日：2007-12-27

申请号：KR1020060056569

申请日：2006-06-22

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김민철 ,  권용국 ,  조성준 ,  이윤정 ,  조영미 ,  권혁만 ,  권준헌 ,  김재홍

IPC: C12Q1/68

CPC classification number: C12N15/11 ,  C12Q1/686 ,  G01N33/569

Abstract: A differential diagnostic method of duck hepatitis virus(DHV) type 1 is provided to improve rapidness and accuracy of diagnosis, and diagnose common genes and different genes of duck hepatitis virus type 1 in the liver tissue separated from young ducks infected by the duck hepatitis virus type 1, and viruses causing other RNA viral diseases derived from fowls. Duck hepatitis virus type 1 gene has the nucleotide sequence consisting of 2C gene, capsid gene and 5'-UTR(untranslated region) gene, wherein the 2C gene has the nucleotide sequence selected from SEQ ID NOs:1, 4, 7, 10, 13, 16, 19, 22 and 25; the capsid gene has the nucleotide sequence selected from SEQ ID NOs:2, 5, 8, 11, 14, 17, 20, 23 and 26; and the 5'-UTR gene has the nucleotide sequence selected from SEQ ID NOs:3, 6, 9, 12, 15, 18, 21, 24 and 27. Duck hepatitis virus-specific primers have the nucleotide sequences of SEQ ID NOs:28 to 33 and specifically recognize the 2C gene, capsid gene and 5'-UTR gene, respectively. A differential diagnostic method of duck hepatitis virus(DHV) type 1 comprises the steps of: (1) homogenizing the liver tissue separated from young ducks infected by the duck hepatitis virus type 1 to float in 10% PBS(phosphate buffered saline); (2) obtaining the supernatant and separating RNA from the supernatant by RNA extraction method; (3) synthesizing cDNA(complementary DNA) from the separated RNA through reverse transcription method by using reverse transcriptase; (4) preparing primers specific to 2C gene, capsid gene and 5'-UTR gene from cDNA, and amplifying the genes through PCR(polymerase chain reaction); and (5) subjecting the PCR products to electrophoresis using 1.5% agarose gel.

Abstract translation: 提供1型鸭肝炎病毒（DHV）的鉴别诊断方法，以提高诊断的快速性和准确性，并在鸭肝炎病毒感染的幼鸭分离的肝组织中诊断1型鸭肝炎病毒的常见基因和不同基因 类型1，以及导致其他来源于家禽的RNA病毒病毒的病毒。 鸭肝炎病毒1型基因具有由2C基因，衣壳基因和5'-UTR（非翻译区）基因组成的核苷酸序列，其中2C基因具有选自SEQ ID NO：1,4,7,10， 13，16，19，22和25; 所述衣壳基因具有选自SEQ ID NO：2,5,8,11,14,17,20,23和26的核苷酸序列; 并且5'-UTR基因具有选自SEQ ID NO：3,6,9,12,15,18,21,24和27的核苷酸序列。鸭肝炎病毒特异性引物具有SEQ ID NO： 28〜33，分别特异性地识别2C基因，衣壳基因和5'-UTR基因。 鸭肝炎病毒（DHV）1型的鉴别诊断方法包括以下步骤：（1）将由1型鸭肝炎病毒感染的幼鸭分离的肝组织匀浆在10％PBS（磷酸盐缓冲盐水）中漂浮; （2）通过RNA提取方法获得上清液和上清液中的RNA; （3）使用逆转录酶通过逆转录法从分离的RNA合成cDNA（互补DNA）; （4）从cDNA制备特异于2C基因，衣壳基因和5'-UTR基因的引物，并通过PCR（聚合酶链式反应）扩增基因; 和（5）使用1.5％琼脂糖凝胶对PCR产物进行电泳。

公开(公告)号：KR100664995B1

公开(公告)日：2007-01-04

申请号：KR1020060002630

申请日：2006-01-10

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권용국 ,  김민철 ,  조성준 ,  이윤정 ,  조영미 ,  권준헌 ,  김재홍

IPC: C12N15/52 ,  C12N15/10

Abstract: A method for diagnosing duck hepatitis virus type 1 is provided to reduce the diagnosis time, improve convenience of diagnosis, and enhance diagnosis accuracy by performing PCR using a primer set specific to duck hepatitis virus type 1. The method for diagnosing duck hepatitis virus type 1 comprises the steps of: (1) pulverizing the liver tissue infected by duck hepatitis virus and suspending the pulverized liver tissue in PBS(phosphate buffer saline); (2) extracting RNA from the supernatant by using LS reagent; (3) synthesizing cDNA from the extracted RNA by using reverse transcriptase(MMLV); (4) performing PCR using synthesized cDNA as a template using 3D gene-specific primers having the nucleotide sequences of SEQ ID NOs:6 and 7; and (5) subjecting the PCR product to electrophoresis by using 1.5% agarose gel, wherein the 3D gene is derived from duck hepatitis virus type 1 and has the nucleotide sequence selected from SEQ ID NO:1 to SEQ ID NO:5.

Abstract translation: 提供一种用于诊断1型鸭肝炎病毒的方法，通过使用特异于1型鸭肝炎病毒的引物组进行PCR，减少诊断时间，提高诊断方便性，提高诊断准确度。1型鸭肝炎病毒诊断方法 包括以下步骤：（1）粉碎鸭肝炎病毒感染的肝组织，并将粉碎的肝组织悬浮于PBS（磷酸盐缓冲盐水）中; （2）使用LS试剂从上清液中提取RNA; （3）使用逆转录酶（MMLV）从提取的RNA合成cDNA; （4）使用具有SEQ ID NO：6和7的核苷酸序列的3D基因特异性引物，使用合成的cDNA作为模板进行PCR; 和（5）使用1.5％琼脂糖凝胶对PCR产物进行电泳，其中3D基因来源于1型鸭型肝炎病毒，并具有选自SEQ ID NO：1至SEQ ID NO：5的核苷酸序列。

公开(公告)号：KR1020060132155A

公开(公告)日：2006-12-21

申请号：KR1020050052325

申请日：2005-06-17

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 성환우 ,  이윤정 ,  최준구 ,  이은경 ,  김재홍

IPC: A61K39/145 ,  A61K39/12

CPC classification number: A61K39/145 ,  A61K38/02 ,  Y10S514/888

Abstract: A low pathogenic avian influenza viral vaccine for preventing H5 subtype avian influenza viral infection is provided to enhance safety and medicinal efficacy of use, and prevent the infection of H5N1 type high pathogenic avian influenza virus safely. The method for preparing the low pathogenic avian influenza viral vaccine for preventing H5 subtype avian influenza viral infection comprises the steps of: (1) culturing a A/wild bird feces/Korea/CSM-2/02(H5N3) strain in a specific pathogen free egg(SPF egg) for 72 hours and collecting allantoic fluid; (2) measuring the titrate of the virus collected in step(1) through hemagglutination inhibition test; (3) concentrating the virus 10 times by centrifuging the virus at 18,000 rpm for 3 hours; (4) attenuating the virus by treating the concentrated virus with 0.1% formalin at 20 deg.C for 12 hours; and (5) mixing the attenuated virus solution with oil adjuvant ISA70 in a weight ratio of 3:7, wherein the low pathogenic avian influenza viral vaccine contains at least one protein encoded by the gene of SEQ ID NO:2 to SEQ ID NO:9.

Abstract translation: 提供用于预防H5亚型禽流感病毒感染的低致病性禽流感病毒疫苗，以提高使用的安全性和药用效力，并安全防止H5N1型高致病性禽流感病毒感染。 制备用于预防H5亚型禽流感病毒感染的低致病性禽流感病毒疫苗的方法包括以下步骤：（1）在特定病原体中培养A /野鸟粪/韩国/ CSM-2/02（H5N3）菌株 免费鸡蛋（SPF蛋）72小时并收集尿囊液; （2）通过血细胞凝集抑制试验测定步骤（1）中收集的病毒的滴度; （3）通过以18,000rpm将病毒离心3小时来浓缩病毒10次; （4）通过用0.1％福尔马林在20℃处理浓缩的病毒12小时来减毒病毒; 和（5）将减毒病毒溶液与油佐剂ISA70以3:7的重量比混合，其中低致病性禽流感病毒疫苗含有由SEQ ID NO：2至SEQ ID NO：2的基因编码的至少一种蛋白质。 9。