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公开(公告)号：AU2005299590A1
公开(公告)日：2006-05-04
申请号：AU2005299590
申请日：2005-10-27
Applicant: CEPHEID
Inventor： CHANG RONALD , DORITY DOUGLAS BRYAN , CHING JESUS , WONG WENDY WINGKEI , ZHANG JIAN PING , ATTA REUEL VAN , PAUL KENDRA LARA , WANG JAMES JIAN QUAN
Abstract: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
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公开(公告)号：CA2587085C
公开(公告)日：2017-08-08
申请号：CA2587085
申请日：2005-10-27
Applicant: CEPHEID
Inventor： CHANG RONALD , CHING JESUS , DORITY DOUGLAS BRYAN , ZHANG JIAN PING , WANG JAMES JIAN QUAN , WONG WENDY WINGKEI , PAUL KENDRA LARA , ATTA REUEL VAN
Abstract: The invention is directed to systems, methods and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use a s a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixture were simply combined together. In the aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
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公开(公告)号：ES2309022T3
公开(公告)日：2008-12-16
申请号：ES01118242
申请日：1998-12-24
Applicant: CEPHEID
Inventor： POURAHMADI FARZAD , MCMILLIAN WILLIAM A , CHING JESUS , CHANG RONALD , CHRISTEL LEE A , KOVACS GREGORY T
IPC: B01J19/00 , G01N31/20 , B01D11/00 , B01L3/00 , B01L7/00 , C12M1/00 , C12M1/26 , C12M1/33 , C12M1/34 , C40B60/14 , G01N1/28 , G01N30/60 , G01N33/50 , G01N35/08 , G01N37/00
Abstract: Cartucho para someter a lisis los componentes de una muestra fluida, presentando el cartucho: a) un orificio de entrada para la introducción de la muestra dentro del cartucho; b) una cámara para lisis que contiene una membrana o filtro para capturar los componentes a medida que la muestra fluye a través de la cámara de lisis; c) un orificio de salida para la salida de la muestra de la cámara de lisis; y d) perlas en la cámara de lisis para la rotura de los componentes; donde la cámara de lisis tiene una pared en la cual puede acoplarse un transductor ultrasónico para realizar la transferencia de energía ultrasónica a la cámara de lisis.
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公开(公告)号：CA2312102C
公开(公告)日：2007-09-04
申请号：CA2312102
申请日：1998-12-24
Applicant: CEPHEID
Inventor： PETERSEN KURT E , POURAHMADI FARZAD , NORTHRUP M ALLEN , CHING JESUS , KOVACS GREGORY T A , CHRISTEL LEE ALLAN , MCMILLAN WIILIAM A , CHANG RONALD
IPC: B01J19/00 , G01N31/20 , B01D11/00 , B01L3/00 , B01L7/00 , C12M1/00 , C12M1/26 , C12M1/33 , C12M1/34 , C12M1/42 , C40B60/14 , G01N1/28 , G01N30/60 , G01N33/50 , G01N35/08 , G01N37/00
Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g., filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41 A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid.
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公开(公告)号：AU2003200701B2
公开(公告)日：2005-12-08
申请号：AU2003200701
申请日：2003-02-27
Applicant: CEPHEID
Inventor： NORTHRUP M ALLEN , POURAHMADI FARZAD , CHING JESUS , CHRISTEL LEE A , PETERSEN KURT E , MCMILLAN WILLIAM A , CHANG RONALD , KOVACS GREGORY T A
IPC: B01J019/00 , B01L003/00 , C12M001/26
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Patent Agency Ranking
公开(公告)号：DE60014676T2
公开(公告)日：2005-11-17
申请号：DE60014676
申请日：2000-05-30
Applicant: CEPHEID SUNNYVALE
Inventor： PETERSEN E , TAYLOR T , POURAHMADI FARZAD , MCMILLAN A , CHANG RONALD , SAKAI H , CHING JESUS , BELGRADER PHILLIP , NORTHRUP ALLEN , DORITY B
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公开(公告)号：AU782343B2
公开(公告)日：2005-07-21
申请号：AU5170700
申请日：2000-05-30
Applicant: CEPHEID
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公开(公告)号：AU780073B2
公开(公告)日：2005-02-24
申请号：AU5301000
申请日：2000-05-30
Applicant: CEPHEID
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公开(公告)号：AT278771T
公开(公告)日：2004-10-15
申请号：AT00936386
申请日：2000-05-30
Applicant: CEPHEID
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公开(公告)号：AU8364801A
公开(公告)日：2002-01-24
申请号：AU8364801
申请日：2001-10-26
Applicant: CEPHEID
Inventor： POURAHMADI FARZAD , PETERSEN KURT E , NORTHRUP M ALLEN , KOVACS GREGORY T A , CHRISTEL LEE A , CHANG RONALD , CHING JESUS , MCMILLAN WILLIAM A
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