公开(公告)号：KR1020030052688A

公开(公告)日：2003-06-27

申请号：KR1020010082715

申请日：2001-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  권병준 ,  고영준 ,  이세영 ,  주이석

IPC: G01N33/68

Abstract: PURPOSE: A VP7 antigen of African horse sickness virus(AHSV) and a diagnostic method of African horse sickness(AHS) using a monoclonal antibody therefor are provided, thereby rapidly and accurately diagnosing AHSV. CONSTITUTION: A diagnostic method of African horse sickness(AHS) using a monoclonal antibody to VP7 antigen of African horse sickness virus(AHSV) comprises the steps of: (1) adhering a monoclonal antibody for VP7 antigen of African horse sickness virus(AHSV) to a solid support; (2) binding the VP7 antigen of AHSV with the monoclonal antibody adhered to the solid support; (3) reacting a sample containing an antibody for a VP7 protein with the VP7 antigen of AHSV; (4) reacting a secondary antibody selected from the group consisting of an enzyme conjugated antibody, a radioactive material conjugated antibody and a fluorescent material conjugated antibody, with the antibody of the step 3; and (5) quantifying the antibody in the sample, wherein the monoclonal antibody for VP7 antigen of African horse sickness virus(AHSV) is produced from a hybridoma cell(KCTC 10136BP).

Abstract translation: 目的：提供非洲马病病毒（AHSV）的VP7抗原和使用其单克隆抗体的非洲马病诊断方法（AHS），从而快速准确地诊断AHSV。 构成：使用非洲马病病毒（AHSV）的VP7抗原单克隆抗体的非洲马病诊断方法（AHS）包括以下步骤：（1）将非洲马病病毒（AHSV）VP7抗原的单克隆抗体 坚定的支持; （2）将AHSV的VP7抗原与附着在固体支持物上的单克隆抗体结合; （3）将含有VP7蛋白抗体的样品与AHSV的VP7抗原反应; （4）将选自酶结合抗体，放射性物质缀合抗体和荧光物质缀合抗体的二抗与步骤3的抗体反应; 和（5）定量样品中的抗体，其中非洲马病病毒（AHSV）的VP7抗原单克隆抗体由杂交瘤细胞（KCTC 10136BP）产生。

公开(公告)号：KR100267748B1

公开(公告)日：2000-10-16

申请号：KR1019980017349

申请日：1998-05-14

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  권병준

IPC: C12N7/02

Abstract: PURPOSE: A method for purifying porcine infectious coronavirus is provided, thereby the porcine infectious coronavirus can be simply and rapidly purified without losing its activity, so that it can be used in producing vaccines or diagnosis kits. CONSTITUTION: The method for purifying porcine infectious coronavirus comprises the steps of: packing the column with crosslinked polysaccharides or sulfate ester of cellulose; washing the column with TEN buffer solution(pH 7.5) consisting of 10mM of Tris, 1mM of EDTA and 100mM of sodium chloride; homogenizing the column with TEN buffer solution; passing the porcine infectious coronavirus containing solution through the column to adsorb it to the column; washing the column with TEN buffer solution; and eluting the porcine infectious coronavirus with TEN buffer solution(pH 7.5) consisting of 10mM of Tris, 1mM of EDTA and 1.0 to 3.0M of sodium chloride.

Abstract translation: 目的：提供猪传染性冠状病毒的纯化方法，可以简单，快速地纯化猪感染性冠状病毒，而不会失去活性，可用于生产疫苗或诊断试剂盒。 构成：猪传染性冠状病毒的纯化方法包括以下步骤：用交联的多糖或纤维素硫酸酯包装柱; 用10mM Tris，1mM EDTA和100​​mM氯化钠组成的TEN缓冲溶液（pH7.5）洗涤柱子; 用TEN缓冲溶液均质柱; 将含有感染性冠状病毒的溶液通过柱吸附到柱上; 用TEN缓冲溶液洗涤柱; 并用10mM Tris，1mM EDTA和1.0-3.0M氯化钠组成的TEN缓冲溶液（pH7.5）洗脱猪感染性冠状病毒。

公开(公告)号：KR101648106B1

公开(公告)日：2016-08-17

申请号：KR1020140051354

申请日：2014-04-29

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 오윤이 ,  김정민 ,  권창희 ,  최은진 ,  송재영 ,  나진주

IPC: C12N15/51 ,  C12N15/85 ,  A61K39/29

CPC classification number: Y02A50/464

Abstract: 본발명은 A형간염바이러스항원및 E형간염바이러스항원동시생산방법및 생산된항원을함유하는간염예방백신및 진단키트에관한것으로, 구체적으로 A형간염바이러스항원으로써 VP1 단백질의일부와 E형간염바이러스항원으로써 ORF2 단백질의일부를곤충세포에서동시에생산하는방법, 이렇게생산된항원을함유함으로써두 가지바이러스에대한항체생성을유도하는예방백신, 및상기항원을함유함으로써피검체에존재하는두 가지바이러스에대한항체를검출할수 있는진단키트에관한것이다. 본발명에따르면 A형간염바이러스항원및 E형간염바이러스항원을동시에안정적으로생산할수 있다. 또한, 생산된항원은이들두 가지바이러스의감염을동시에예방하기위한백신이나감염여부를진단하기위한키트로유용하게사용할수 있다. 상기바이러스들이가축으로부터감염될수 있는만큼, 본발명의백신과키트를사용하여가축을관리하면바이러스보유가능성을차단할수 있어사람에게전이되는것을사전에방지할수 있을것으로판단되며, 심각한간염을일으키는동시감염도예방할수 있어질병관리에큰 도움을줄 것으로기대된다.

公开(公告)号：KR1020150125049A

公开(公告)日：2015-11-09

申请号：KR1020140051354

申请日：2014-04-29

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 오윤이 ,  김정민 ,  권창희 ,  최은진 ,  송재영 ,  나진주

IPC: C12N15/51 ,  C12N15/85 ,  A61K39/29

CPC classification number: Y02A50/464 ,  C07K14/02 ,  A61K39/29 ,  C12N15/85

Abstract: 본발명은 A형간염바이러스항원및 E형간염바이러스항원동시생산방법및 생산된항원을함유하는간염예방백신및 진단키트에관한것으로, 구체적으로 A형간염바이러스항원으로써 VP1 단백질의일부와 E형간염바이러스항원으로써 ORF2 단백질의일부를곤충세포에서동시에생산하는방법, 이렇게생산된항원을함유함으로써두 가지바이러스에대한항체생성을유도하는예방백신, 및상기항원을함유함으로써피검체에존재하는두 가지바이러스에대한항체를검출할수 있는진단키트에관한것이다. 본발명에따르면 A형간염바이러스항원및 E형간염바이러스항원을동시에안정적으로생산할수 있다. 또한, 생산된항원은이들두 가지바이러스의감염을동시에예방하기위한백신이나감염여부를진단하기위한키트로유용하게사용할수 있다. 상기바이러스들이가축으로부터감염될수 있는만큼, 본발명의백신과키트를사용하여가축을관리하면바이러스보유가능성을차단할수 있어사람에게전이되는것을사전에방지할수 있을것으로판단되며, 심각한간염을일으키는동시감염도예방할수 있어질병관리에큰 도움을줄 것으로기대된다.

Abstract translation: 本发明涉及一种同时产生甲型肝炎病毒抗原和戊型肝炎病毒抗原的方法，以及预防肝炎疫苗和含有产生抗原的诊断试剂盒。 特别地，本发明涉及：作为甲型肝炎病毒抗原的VP1蛋白的一部分和来自昆虫细胞的作为戊型肝炎病毒抗原的部分ORF2蛋白的方法。 用于通过含有产生的抗原诱导产生针对两种病毒的抗原的预防疫苗; 以及用于通过含有抗原来检测存在于受试者中的两种病毒的抗体的诊断试剂盒。 根据本发明，能够同时且稳定地制造甲型肝炎病毒抗原和戊型肝炎病毒抗原。 此外，产生的抗原可以有用地用作同时预防两种病毒感染的疫苗，或用作诊断感染的试剂盒。 病毒可以从家畜感染，因此当本发明的疫苗和试剂盒用于管理家畜时，可以阻止病毒的保存。 因此，可以预先防止向人的过渡。 也可以预防同时感染引起严重肝炎的感染，因此预期本发明将有助于控制疾病。

公开(公告)号：KR1020140081399A

公开(公告)日：2014-07-01

申请号：KR1020120151098

申请日：2012-12-21

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 나진주 ,  권창희 ,  오윤이 ,  송재영

IPC: C12N15/51 ,  C07K14/005 ,  C12N15/866 ,  G01N33/53

Abstract: The present invention relates to a recombinant cell for expressing a capsid protein of swine hepatitis E virus. The present invention can be effectively used as a vaccine against swine hepatitis E virus by producing the capsid protein remarkably similar to the state of swine hepatitis E virus infection, which is difficult to artificially culture within a laboratory. Considering the difficulty in performing artificial culturing of the virus, the capsid protein can be remarkably useful in precise disease diagnosis since it can be used in a final diagnosis antibody test method.

Abstract translation: 本发明涉及用于表达猪戊型肝炎病毒衣壳蛋白的重组细胞。 通过产生与实验室难以人工培养的猪戊型肝炎病毒感染状态非常相似的衣壳蛋白，可以有效地利用本发明作为针对猪型戊型肝炎病毒的疫苗。 考虑到难以进行病毒的人造培养，因为可以在最终的诊断抗体试验方法中使用，所以衣壳蛋白质可以在精确的疾病诊断中显着有用。

公开(公告)号：KR1020130077958A

公开(公告)日：2013-07-10

申请号：KR1020110146641

申请日：2011-12-30

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  오윤이 ,  이경기 ,  김연희 ,  송재영

IPC: C07K16/08 ,  G01N33/569 ,  G01N33/53 ,  A61K39/12

Abstract: PURPOSE: A porcine circovirus 2 (PCV2) capsid protein is provided to produce a large amount of antigens, to diagnose circovirus 2 capsid antibody, and to produce a vaccine. CONSTITUTION: A method for detecting a porcine circovirus antibody comprises a step of using a porcine circovirus 2 capsid protein as an antigen. The capsid protein has an amino acid sequence of sequence number 2. The amino acid sequence is encoded by a base sequence of sequence number 1. The method is performed by a fluorescent antibody test or enzyme immunoassay. A vaccine for preventing porcine circovirus 2 contains the capsid protein as an antigen.

Abstract translation: 目的：提供猪圆环病毒2（PCV2）衣壳蛋白以产生大量抗原，诊断圆环病毒2衣壳抗体并产生疫苗。 构成：检测猪圆环病毒抗体的方法包括使用猪圆环病毒2衣壳蛋白作为抗原的步骤。 衣壳蛋白具有序列号2的氨基酸序列。氨基酸序列由序列号1的碱基序列编码。该方法通过荧光抗体测试或酶免疫测定进行。 用于预防猪圆环病毒2的疫苗含有衣壳蛋白作为抗原。

公开(公告)号：KR1020130077295A

公开(公告)日：2013-07-09

申请号：KR1020110145925

申请日：2011-12-29

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 강승원 ,  최세은 ,  노진형 ,  권창희 ,  고경봉

IPC: C07K16/08 ,  A61K39/395 ,  C12N5/07

Abstract: PURPOSE: An immunoglobulin yolk according to a bee virus is provided to be used as a controlling method of environment-friendly bee virus by inducing an immune reaction in the bee and to replace chemical controlling method or a conventional post antigen detecting method. CONSTITUTION: A manufacturing method of immunoglobulin yol according to a bee virus comprises 1) a step of inoculating the bee virus as an antigen to an egg, 2) a step fo collecting yolk from the egg, and 3) a step of separating the immunoglobulin yolk according to the bee virus from the yolk. The inoculation is performed 0.1ml to 0.5ml for the first inoculation and for two weeks apart, 2-4 times are inoculated. A composition for the bee virus prevention comprises the bee virus immunoglobulin yolk.

Abstract translation: 目的：提供一种根据蜂病毒的免疫球蛋白，通过诱导蜜蜂中的免疫反应，替代化学治疗方法或常规的后抗原检测方法，将其用作环境友好的蜂病毒的控制方法。 构成：根据蜂病毒的免疫球蛋白的制造方法包括：1）将作为抗原的蜂病毒接种于卵的步骤，2）从卵收集蛋黄的步骤，3）分离免疫球蛋白的步骤 根据来自蛋黄的蜂病毒的蛋黄。 接种0.1ml至0.5ml进行第一次接种，间隔两周，接种2-4次。 用于预防蜂病毒的组合物包括蜂病毒免疫球蛋白蛋黄。

公开(公告)号：KR1020130077232A

公开(公告)日：2013-07-09

申请号：KR1020110145828

申请日：2011-12-29

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 강승원 ,  최세은 ,  노진형 ,  권창희 ,  이희수

IPC: C12N7/00 ,  C12N5/02 ,  G01N33/15

Abstract: PURPOSE: A culturing method of bee virus using a bee larva is provided to be usefully used on screening effective treatment by evaluating effect of the therapeutic agent according to the various bee virus using effectively cultured bee virus which was hard to culture in-vitro. CONSTITUTION: A culturing method of bee virus is to infect the bee virus to artificially cultivated bee larva. The articifial culturing is performed on the bee larva at 35-40 degree Celsius and 80-90% humidity. The culturing comprises 5-10% of D-glucose, 5-10% of D-fructose, 1-3% of enzyme extract, 25-35% of grace medium, and 80-100 micro liters of solution comprised of 40-50% of royal jelly. A bee virus treating material screening method using the artificially cultured larva comprises 1) a step of artificially culturing the bee larva in-vitro, 2) a step of culturing a separated bee virus to the larva from 1), and 3) a step of measuring concentration of virus after treating the bee virus therapeutic agent. [Reference numerals] (AA) Virus infected group; (BB) Thymol and chlorine dioxide treated group; (CC) Virus non-infected group; (DD) Survival rate (%); (EE) Cultivated days

Abstract translation: 目的：提供使用蜜蜂幼虫的蜂毒病毒培养方法，通过使用有效培养的体外培养的蜜蜂病毒评估各种蜂病毒治疗剂的效果，有效地用于筛选有效的治疗。 构成：蜜蜂病毒的培养方法是将蜜蜂病毒感染到人工培养的蜜蜂幼虫。 在35-40摄氏度和80-90％湿度的蜜蜂幼虫进行表达培养。 培养包括5-10％的D-葡萄糖，5-10％的D-果糖，1-3％的酶提取物，25-35％的宽带培养基和80-100微升的溶液组成的40-50 ％的蜂王浆。 使用人造培养的幼虫的蜂病毒处理材料筛选方法包括1）在体外人工培养蜜蜂幼体的步骤，2）从1）培养分离的蜜蜂病毒至幼虫的步骤，以及3）步骤 治疗蜂病毒治疗剂后测定病毒浓度。 （附图标记）（AA）病毒感染组; （BB）百里酚和二氧化氯处理组; （CC）病毒非感染组; （DD）生存率（％）; （EE）耕种日

公开(公告)号：KR101086245B1

公开(公告)日：2011-11-29

申请号：KR1020090020979

申请日：2009-03-12

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 고영준 ,  이향심 ,  허은정 ,  권창희

IPC: C07K16/08 ,  C12N5/20 ,  G01N33/53 ,  A61K39/42

Abstract: 본 발명은 수포성구내염바이러스 뉴저지형 당단백질에 결합하는 단일클론항체 및 이의 용도에 관한 것으로, 보다 자세하게는 수포성구내염바이러스 뉴저지형의 당단백질에 결합하는 단일클론항체, 이를 생산하는 융합세포주 및 상기 단일클론항체와 수포성구내염바이러스 뉴저지형의 당단백질을 이용하여 수포성구내염바이러스 뉴저지형에 특이적인 중화항체를 검출할 수 있는 수포성구내염 진단방법에 관한 것이다.
수포성구내염바이러스 뉴저지형, 단일클론항체

公开(公告)号：KR1020100006868A

公开(公告)日：2010-01-22

申请号：KR1020080067171

申请日：2008-07-10

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 정병열 ,  이경기 ,  박최규 ,  엄재구 ,  최정수 ,  송윤경 ,  강민수 ,  김애란 ,  이경우 ,  문운경 ,  권준헌 ,  권창희

IPC: G01N30/02 ,  G01N30/00

Abstract: PURPOSE: A diagnostic kit for rapid detection of rabies antibodies is provided to rapidly detect the existence according to rabies vaccination within 20 minutes without expensive equipment and to reduce the generation of rabies. CONSTITUTION: A diagnostic kit for rapid detection of rabies antibodies using immunochromatography has a nitrocellulose membrane(30) containing a test line(40) and a control line(50) on a backing card(70). A conjugate pad is overlapped at one side of the nitrocellulose membrane. A sample pad(10) is overlapped on the conjugate pad. An adsorbent pad(60) absorbing blood serum in which the reaction is completed on a backing card is installed at the other side of the nitrocellulose membrane.

Abstract translation: 目的：提供用于快速检测狂犬病抗体的诊断试剂盒，用于在20分钟内快速检测狂犬病疫苗的存在，无需昂贵的设备，并减少狂犬病的产生。 构成：用于使用免疫层析法快速检测狂犬病抗体的诊断试剂盒具有在背板（70）上含有测试线（40）和对照线（50）的硝酸纤维素膜（30）。 缀合垫在硝酸纤维素膜的一侧重叠。 样品垫（10）重叠在共轭垫上。 在硝酸纤维素膜的另一侧安装吸收血清的吸附垫（60），其中在背衬卡上完成反应。