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    CHEMICAL PROCESSING DEVICE
    42.
    发明专利
    CHEMICAL PROCESSING DEVICE 未知

    公开(公告)号：CA2324244A1

    公开(公告)日：1999-09-23

    申请号：CA2324244

    申请日：1999-03-17

    Applicant: CEPHEID

    Inventor： MCMILLAN WILLIAM A ,  PETERSEN KURT E ,  NORTHRUP M ALLEN ,  KOVACS GREGORY T A

    IPC: B03C5/00 ,  B01D57/02 ,  B01J19/00 ,  B01L3/00 ,  B01L99/00 ,  G01N27/447 ,  G01N30/60

    Abstract: A sample-processing device (150) comprises a unitary body (152), preferably a molded polymeric part, having formed therein a reaction chamber (154) for chemically reacting a sample, a separation region (158) for separating components of the sample, and a transition region (156) connecting the reaction chamber (154) to the separation region (158). The reaction chamber (154), transition region (156), and separation region (158) are formed in and enclosed by the unitary body (152). Additionally, the transition region (156) includes at least one flow restrictor (180) for controlling the flow of fluid between the reaction chamber (154) and the separation region (158). Further, the portion of the unitary body (152) defining the transition region (156) has lower thermal conduction than the portion of the body defining the reaction chamber (154) so that the transition region (156) thermally isolates the reaction chamber (154) from the separation region (156). In a preferred embodiment, the reaction chamber (154) is an amplification chamber for amplifying nucleic acid in the sample, and the separation region (158) comprises an electrophoresis column or capillary containing a suitable matrix material, such as electrophoresis gel or buffer, for separating nucleic acid fragments in the sample. Electrodes (167, 168, 169) are embedded in the body (152) for forcing the sample to flow from the reaction chamber (154) to the separation region (158). The unitary body (152) may also be surrounded by external, functional components such as an optical detector (186) for detecting separated components of the sample.

    CARTUCHO PARA REALIZAR UNA REACCION QUIMICA.
    46.
    发明专利
    CARTUCHO PARA REALIZAR UNA REACCION QUIMICA. 未知

    公开(公告)号：ES2272289T3

    公开(公告)日：2007-05-01

    申请号：ES00937894

    申请日：2000-05-30

    Applicant: CEPHEID

    Inventor： PETERSEN KURT E ,  MCMILLAN WILLIAM A ,  POURAHMADI FRAZAD ,  CHANG RONALD ,  DORITY DOUGLAS B

    IPC: B01L3/00 ,  G01N33/48 ,  B01J19/00 ,  B01J19/24 ,  B01L7/00 ,  C12M1/00 ,  C12M1/26 ,  G01N33/483 ,  G01N35/08

    Abstract: Cartucho para realizar una reacción química que comprende un cuerpo que tiene al menos un primer canal y un segundo canal [80, 81] ubicados en su interior, que se caracterizan porque: dicho cartucho comprende además un recipiente de reacción [40] que se extiende desde el cuerpo, teniendo el recipiente de reacción [40]: (i) una cámara de reacción [42]; (ii) un puerto de entrada [41] conectado a la cámara de reacción [42] a través de un canal de entrada [50]; y (iii) un puerto de salida [43] conectado a la cámara de reacción a través de un canal de salida [52]; en el que la puerta de entrada [41] del recipiente [40] está conectado al primer canal [80] en el cuerpo y en el que la puerta de salida [43] del recipiente [40] está conectado al segundo canal [81] del cuerpo.

    50.
    发明专利
    未知

    公开(公告)号：AT228242T

    公开(公告)日：2002-12-15

    申请号：AT99925705

    申请日：1999-05-19

    Applicant: CEPHEID

    Inventor： CHRISTEL LEE A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  MCMILLAN WILLIAM A ,  KOVACS GREGORY T A ,  YOUNG STEVEN J ,  CHANG RONALD ,  DORITY DOUGLAS B ,  HEBERT RAYMOND ,  KINTZ GREGORY J

    IPC: G01N21/64 ,  G01N21/01 ,  G01N21/03

    Abstract: An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel (2) having a chamber (10) for holding the mixture. The apparatus also includes a heat-exchanging module (37) having a pair of opposing thermal plates (34A, 34B) for receiving the vessel (2) between them and for heating and/or cooling the mixture contained in the vessel. The module (37) also includes optical excitation and detection assemblies (46, 48) positioned to optically interrogate the mixture. The excitation assembly (46) includes multiple light sources (100) and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly (48) includes multiple detectors (102) and a second set of filters for detecting light emitted from the chamber (10) in multiple emission wavelength ranges. The optics assemblies (46, 48) thus provide a multi-channel system for detcting a plurality of different target analytes in the mixture.

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