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公开(公告)号：KR1020130046048A
公开(公告)日：2013-05-07
申请号：KR1020110110371
申请日：2011-10-27
Applicant: 강원대학교산학협력단
CPC classification number: C12N15/8205 , A01H4/001 , A01H4/005 , A01H4/008 , C12N5/0025 , C12N15/8209 , C12N15/8241 , C12N2500/34 , C12N2501/30
Abstract: PURPOSE: A method for producing a transgenic Miscanthus plant is provided to obtain Miscanthus plants with high transformation efficiency using callus. CONSTITUTION: A method for producing a transgenic Miscanthus plant comprises: a step of placing a Miscanthus seed on a callus-inducing medium and inducing Miscanthus callus; a step of co-culturing the induced Miscanthus callus and Agrobacterium tumefaciens containing a foreign gene-inserted plant expression vector; a step of culturing the co-cultured plant cells in a selective medium containing antibiotics; a step of elongating the selected Miscanthus cells in an elongation medium; and a step of inducing rooting of the elongated cells in a medium for inducing rooting.
Abstract translation: 目的：提供一种生产转基因芒草植物的方法，以使用愈伤组织获得高转化效率的芒草。 构成：用于生产转基因芒草植物的方法包括：将芒草种子置于愈伤组织诱导培养基上并诱导芒草的步骤; 共培养含有外源基因插入植物表达载体的诱导的芒草和根癌土壤杆菌的步骤; 在含有抗生素的选择培养基中培养共培养的植物细胞的步骤; 在延伸培养基中拉长所选的芒草细胞的步骤; 以及在用于诱导生根的培养基中诱导细长细胞的生根的步骤。
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公开(公告)号：KR101014202B1
公开(公告)日：2011-02-14
申请号：KR1020080031373
申请日：2008-04-03
Applicant: 강원대학교산학협력단
Abstract: 포도를 원적외선으로 40 내지 120℃에서 5 내지 60분간, 바람직하게는 70℃에서 30분간 조사하여 항산화 활성이 증가된 건포도를 생산하는 방법에 관한 것으로, 원적외선 처리된 포도를 이용하여 저장 중 산화를 억제하여 맛과 향이 우수한 건포도를 제조할 수 있다.
포도, 원적외선, 항산화, 건포도-
Patent Agency Ranking
公开(公告)号：KR1020090105739A
公开(公告)日：2009-10-07
申请号：KR1020080031373
申请日：2008-04-03
Applicant: 강원대학교산학협력단
Abstract: PURPOSE: A raisin with increased antioxidant activity and a preparation method thereof are provided to remove oxidized flavor during preservation, thereby exhibiting pleasing taste and flavor. CONSTITUTION: A method for preparing a raisin with increased antioxidant activity through a far-infrared drying process comprises the following steps of: irradiating far infrared rays to grapes; and drying them to obtain the raisin with increased antioxidant activity. The far infrared rays emit a wavelength within a range of 2 ~ 14 μm. The far infrared rays are irradiated at 40 ~ 120°C for 5-60 minutes. The increase in antioxidant activity represents the increase in polyphenol content. The polyphenol is selected from the group consisting of catechin, epicatechin, epigallocatechin gallate, gallic acid, resveratrol, epigallocatechin, and quercetin.
Abstract translation: 目的：提供抗氧化活性增强的葡萄干及其制备方法，以在保存过程中除去氧化味，从而表现出令人愉快的味道和风味。 构成：通过远红外干燥方法制备具有增加的抗氧化活性的葡萄干的方法包括以下步骤：将远红外线照射到葡萄; 并将其干燥以获得具有增加的抗氧化活性的葡萄干。 远红外线发射波长在2〜14μm的范围内。 远红外线在40〜120℃照射5-60分钟。 抗氧化活性的增加代表多酚含量的增加。 多酚选自儿茶素，表儿茶素，表没食子儿茶素没食子酸酯，没食子酸，白藜芦醇，表没食子儿茶素和槲皮素组成的组。
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公开(公告)号：KR100835710B1
公开(公告)日：2008-06-09
申请号：KR1020070095740
申请日：2007-09-20
Applicant: 강원대학교산학협력단
Abstract: 본 발명은 케나프 추출물과 이로부터 분리된 신규 캠프페롤 화합물 및 이의 식품으로서의 용도에 관한 것으로서, 더욱 상세하게는 케나프(
Hibiscus
cannabbinus L., Malvaceae) 추출물과 이로부터 분리된 신규 캠프페롤 화합물이 항균 및 항산화 활성을 나타냄을 밝히고, 이를 식품으로 적용하기 위한 새로운 용도에 관한 것이다.
케나프(Hibiscus cannabbinus L., Malvaceae), 캠프페롤 화합물-
公开(公告)号：KR100815439B1
公开(公告)日：2008-03-20
申请号：KR1020070014897
申请日：2007-02-13
Applicant: 강원대학교산학협력단
CPC classification number: A01H4/005 , A01H4/001 , A01H4/008 , C12N5/0025 , C12N2500/34 , C12N2501/30
Abstract: A method for mass-producing an Oplopanax elatus Nakai plantlet is provided to mass-propagate the Oplopanax elatus Nakai effectively from embryogenetic callus derived from an Oplopanax elatus Nakai tissue, thereby preserving endangered Oplopanax elatus Nakai. A method for mass-producing an Oplopanax elatus Nakai plantlet comprises the steps of: (a) explanting a tissue segment of Oplopanax elatus Nakai a cell tissue cultures in an MS culture medium where a plant growth control material including TDZ is added to induce embryogenetic callus; (b) culturing the induced embryogenetic callus in a liquid culture medium or a solid culture medium to induce somatic embryos; (c) culturing the induced somatic embryos in a liquid culture medium or a solid culture medium to propagate them; (d) culturing the propagated somatic embryos in a liquid culture medium or a solid culture medium having a pH of 5.0-5.75 to redifferentiate a plantlet; and (e) acclimatizing the redifferentiated plantlet in a soil where bed soil and sand are mixed in a ratio of 1:1, wherein the culture medium of the step(a) is an MS culture medium where 2,4-D or NAA is further added as the plant growth controlling material. Further, the MS culture medium comprises 10-30 mg/l of sucrose as carbon source.
Abstract translation: 提供了一种大规模生产Oplopanax elatus Nakai小植物的方法，有效地从Oplopanax elatus Nakai组织的胚胎发生愈伤组织中大量繁殖Oplopanax elatus Nakai，从而保存濒危的Oplopanax elatus Nakai。 一种大规模生产Oplopanax elatus Nakai小植物的方法包括以下步骤：（a）在MS培养基中移除Oplopanax elatus Nakai的组织段，其中加入包含TDZ的植物生长控制材料以诱导胚胎发生愈伤组织 ; （b）在液体培养基或固体培养基中培养诱导的胚胎发生愈伤组织以诱导体细胞胚; （c）在液体培养基或固体培养基中培养诱导的体细胞胚以繁殖它们; （d）在液体培养基或pH5.0-5.75的固体培养基中培养繁殖的体细胞胚以再分化植物; 和（e）将所述再分化的小植物驯化在比例为1:1的床土壤和沙子的土壤中，其中步骤（a）的培养基是MS培养基，其中2,4-D或NAA为 进一步添加作为植物生长控制材料。 此外，MS培养基包含10-30mg / l蔗糖作为碳源。
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公开(公告)号：KR1020070105812A
公开(公告)日：2007-10-31
申请号：KR1020060067626
申请日：2006-07-19
Applicant: 강원대학교산학협력단
IPC: A61K36/185 , A23L1/29
Abstract: An extract of Hibiscus cannabinus L.(Malvaceae) and a novel kaempferol compound isolated therefrom are provided to inhibit growth of microorganisms, oxidation and aging in the skin without side effects, so that they are useful in medicines, cosmetics and functional food for improving skin beauty. An extract of Hibiscus cannabinus L. having improved antimicrobial and antioxidizing effects is prepared by extracting Hibiscus cannabinus L. with a solvent selected from water, aliphatic alcohol or alcohol solution, hexane, chloroform and ethylacetate. A novel kaempferol compound represented by the formula(1) is isolated from the extract of Hibiscus cannabinus L. by extracting Hibiscus cannabinus L. with methanol, extracting the crude methanol extract with water, aliphatic alcohol, alcohol solution, hexane, chloroform or ethylacetate to obtain a water layer and an organic layer, and subjecting the water layer and organic layer to silica chromatography, gel filtration chromatography and HPLC(high performance liquid chromatography), sequentially.
Abstract translation: 提供木槿大麻提取物和从其中分离出的新型山奈酚化合物，以抑制微生物的生长，皮肤的氧化和老化，而不会有副作用，因此它们可用于改善皮肤的药物，化妆品和功能性食品 美丽。 通过用选自水，脂族醇或醇溶液，己烷，氯仿和乙酸乙酯的溶剂萃取木槿大麻制备具有改善的抗微生物和抗氧化作用的木槿提取物。 用甲醇提取木槿大麻，从木槿提取物中分离出由式（1）表示的新型山奈酚化合物，用水，脂肪醇，醇溶液，己烷，氯仿或乙酸乙酯提取粗甲醇提取物， 得到水层和有机层，依次将水层和有机层进行硅胶色谱，凝胶过滤色谱和HPLC（高效液相色谱）。
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公开(公告)号：KR1020070105811A
公开(公告)日：2007-10-31
申请号：KR1020060067602
申请日：2006-07-19
Applicant: 강원대학교산학협력단
Abstract: Healthy tea with kenaf leaves and a preparation method thereof are provided to facilitate the elution of active ingredients in extraction in hot water by treating the leaves with heat and far infrared ray in a fixed condition, thereby substituting the same for green tea. Healthy tea with kenaf leaves is prepared by the steps of: heating kenaf leaves at 60~100°C for 10~45min; and applying far infrared ray to the leaves at 1.6kw for 10~45min while keeping the temperature at 60~100°C. In the preparation process, large quantities of minerals and antioxidant ingredients are extracted from the kenaf leaves. The extract is especially rich in kaempferols as a polyphenol compound. In addition, the content of physiologically active ingredients in the kenaf leaves is higher than that in green tea leaves.
Abstract translation: 提供具有洋麻叶的健康茶及其制备方法，以便通过用固定状态的热和远红外线处理叶子以促进在热水中萃取中的活性成分的洗脱，从而将其替换为绿茶。 通过以下步骤制备具有红麻叶的健康茶：在60〜100℃下加热洋麻叶10〜45分钟; 并在1.6kw的条件下将远红外线应用10〜45min，同时保持温度在60〜100℃。 在制备过程中，从洋麻叶中提取大量的矿物质和抗氧化成分。 提取物特别富含作为多酚化合物的山奈酚。 此外，洋麻叶中生理活性成分的含量高于绿茶叶。
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