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    흑염소 반추위 미생물 유래의 섬유소분해효소 ｃｅｌ―ＫＧ２７ 유전자 및 이의 용도
    51.
    发明公开
    흑염소 반추위 미생물 유래의 섬유소분해효소 ｃｅｌ―ＫＧ２７ 유전자 및 이의 용도 有权
    来自黑山羊RUMEN MICROORGANISM的细胞因子CEL-KG27基因及其用途

    公开(公告)号：KR1020140064541A

    公开(公告)日：2014-05-28

    申请号：KR1020120131968

    申请日：2012-11-20

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 이경태 ,  김태헌 ,  김경운 ,  변미정 ,  안정현 ,  김영주 ,  안현승 ,  김지훈

    IPC: C12N15/56 ,  C12N9/42 ,  C12N15/63 ,  A23K1/16

    Abstract: The present invention relates to a fibrinogenase cel-KG27 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG27 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG27 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG27 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.

    Abstract translation: 本发明涉及一种来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG27基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因的cel-KG27基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG27基因，通过提供纤维蛋白原酶cel-KG27基因和蛋白质产物，可以用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。

    3Ｄ8 ｓｃＦｖ 유전자가 도입된 형질전환 닭 및 그 제조방법
    54.
    发明公开
    3Ｄ8 ｓｃＦｖ 유전자가 도입된 형질전환 닭 및 그 제조방법 无效
    含有3D8 SCFV基因的转基因鸡肉及其生产方法

    公开(公告)号：KR1020120079389A

    公开(公告)日：2012-07-12

    申请号：KR1020110000639

    申请日：2011-01-04

    Applicant: 대한민국(농촌진흥청장) ,  성균관대학교산학협력단 ,  아주대학교산학협력단

    Inventor： 변승준 ,  권명희 ,  이석찬 ,  김점순 ,  김경운 ,  김성우 ,  류재규 ,  박수봉 ,  전익수

    IPC: C12N15/85 ,  C12N15/12 ,  A01K67/027 ,  C12Q1/70

    Abstract: PURPOSE: A transgenic chicken and a method for producing the same are provided to ensure anti-viral ability and to be used as a model animal for preventing viral diseases. CONSTITUTION: A vector for transformation contains a promoter which is operable in birds and a 3D8 scFv gene which is operatively linked to the promoter. The promoter is a chicken beta-actin gene promoter. The 3D8 scFv gene further contains a reporter gene. The reporter gene is HA-tag. A method for producing a transgenic chicken comprises: a step of preparing the vector; a step of transducing the vector to chicken fertilized eggs; and a step of hatching the fertilized eggs.

    Abstract translation: 目的：提供转基因鸡及其制备方法以确保抗病毒能力，并用作预防病毒性疾病的示范动物。 构成：用于转化的载体包含可在鸟类中操作的启动子和与启动子有效连接的3D8scFv基因。 启动子是鸡β-肌动蛋白基因启动子。 3D8 scFv基因还含有报告基因。 报告基因是HA标签。 一种生产转基因鸡的方法包括：制备载体的步骤; 将载体转导到鸡受精卵的步骤; 和孵化受精卵的步骤。

    단백질 과발현 카세트를 포함하는 유전자 타겟팅 넉인 벡터, 이의 제조 방법 및 이 벡터가 도입된 이종간 이식용 형질전환 복제동물
    55.
    发明公开
    단백질 과발현 카세트를 포함하는 유전자 타겟팅 넉인 벡터, 이의 제조 방법 및 이 벡터가 도입된 이종간 이식용 형질전환 복제동물 有权
    含有过表达CASSETTE的基因靶向插入载体，构建其的方法和用于XENOTRANSPLANTATION的载体的转基因克隆动物

    公开(公告)号：KR1020120055409A

    公开(公告)日：2012-05-31

    申请号：KR1020100117142

    申请日：2010-11-23

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 오건봉 ,  황성수 ,  임기순 ,  곽태욱 ,  우제석 ,  양병철 ,  고응규 ,  김경운 ,  박수봉 ,  강만종

    IPC: C12N15/85 ,  C12N15/54 ,  A01K67/027

    Abstract: PURPOSE: A gene targeting knock-in vector including protein over-revelation cassette, a manufacturing method thereof, and a transformation cloned animal for heterograft in which the vector is introduced are provided to manufacture a transformation reproduction miniature reproduction pig in which a graft rejection is controlled by effectively knock-out and knock-in at the same time. CONSTITUTION: A gene targeting knock-in vector successively including protein over-revelation cassette comprises a first area having base sequences which are homologous in gene of mammal, a cassette for protein over-revelation, and a second area having homologous base sequences in gene of mamma. The gene of mammal is alpha 1,3- galactosyltransferase gene. The mammal is a pig. The pig is a NH-miniature pig. The first area is a left arm including a part of intron 8 of the alpha 1,3- galactosyltransferase or Exxon 9. A size of the first area is 2kb~6kb.

    Abstract translation: 目的：提供靶向敲入载体的基因，包括蛋白质过度启动盒，其制备方法和引入载体的异种移植物的转化克隆动物，以制备其中移植排斥反应为 通过有效地敲除和敲门控制在同一时间。 构成：连续包含蛋白质超启录盒的靶向敲入载体的基因包括具有在哺乳动物基因中同源的碱基序列的第一区域，用于蛋白质过度启动的盒，以及在基因中具有同源碱基序列的第二区域 妈妈。 哺乳动物的基因是α-1,3-半乳糖基转移酶基因。 哺乳动物是猪。 猪是NH型微型猪。 第一区域是包含α-1,3-半乳糖基转移酶或Exxon 9的内含子8的一部分的左臂。第一区域的大小为2kb〜6kb。

    인간 과립구 콜로니 자극인자의 생물학적 활성 증가 변이체
    56.
    发明公开
    인간 과립구 콜로니 자극인자의 생물학적 활성 증가 변이체 有权
    具有增加的生物活性的突变C-CSF生产

    公开(公告)号：KR1020110078038A

    公开(公告)日：2011-07-07

    申请号：KR1020090134751

    申请日：2009-12-30

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 김성우 ,  변승준 ,  김경운 ,  이휘철 ,  고은미 ,  정희경 ,  박진기 ,  류재규 ,  박수봉

    IPC: C07K14/535 ,  C12N15/27 ,  C12N15/63 ,  A61K38/18

    Abstract: PURPOSE: A mutant human G-CSF with increased biological activity is provided to ensure superior biological activity and excellent economic effect with low dosage. CONSTITUTION: A mutant human G-CSF has amino acid modification by N-glycosylateion by formation of Asn-X-Ser/Thr(N-X-S/T) sequence in an amino acid residues: T1-P10 (T1-P-L-Q-P-A-S-S-L-P10); K40-Q70 (K40-L-C-H-P-E-E-L-V-L-L-G-H-S-L-G-I-P-W-A-P-L-S-S-C-P-S-Q-A-L-Q70); or L124-S142 (L124-G-M-A-P-A-L-Q-P-T-Q-G-A-M-P-A-F-A-S142). A pharmaceutical composition contains the mutant human G-CSF.

    Abstract translation: 目的：提供具有增加生物活性的突变型人类G-CSF，以确保优异的生物活性和低剂量的优异的经济效果。 构成：突变型人G-CSF通过在氨基酸残基：T1-P10（T1-P-L-Q-P-A-S-S-L-P10）中形成Asn-X-Ser / Thr（N-X-S / T）序列而进行N-糖基化的氨基酸修饰。 K40-Q70（K40-L-C-H-P-E-E-L-V-L-L-G-H-S-L-G-I-P-W-A-P-L-S-S-C-P-S-Q-A-L-Q70） 或L124-S142（L124-G-M-A-P-A-L-Q-P-T-Q-G-A-M-P-A-F-A-S142）。 药物组合物含有突变型人G-CSF。

    흑염소 반추위 미생물 유래의 엔도-1,4-베타-자일라나아제 cel10-KG99 유전자 및 이의 용도
    60.
    发明授权
    흑염소 반추위 미생물 유래의 엔도-1,4-베타-자일라나아제 cel10-KG99 유전자 및 이의 용도 有权
    源自黑山羊瘤胃微生物的内切-1,4-β-木聚糖酶cel10-KG99基因及其用途

    公开(公告)号：KR101816620B1

    公开(公告)日：2018-01-10

    申请号：KR1020150191015

    申请日：2015-12-31

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 이경태 ,  임다정 ,  김경운 ,  박미림

    IPC: C12N9/24 ,  C12N15/52 ,  C12N15/63 ,  A23K1/165 ,  C11D3/386 ,  C12P7/64

    Abstract: 본발명은흑염소반추위미생물유래의엔도-1,4-베타-자일라나아제 cel10-KG99 유전자및 이의용도에관한것으로, 보다구체적으로흑염소반추위미생물로부터선별한신규한엔도-1,4-베타-자일라나아제 cel10-KG99 유전자및 이의단백질산물을제공하고, 이를이용하여사료첨가제, 세제조성물및 바이오연료를제조하는방법에관한것이다.

    Abstract translation: 瘤胃的发明山羊日元微生物图β-1,4-二甲苯蛙cel10氮杂KG99基因和涉及它们的用途，hansingyu汉恩从山羊瘤胃微生物选择的并且更具体地如图1，图4的β- 贾尔斯拉纳cel10氮杂KG99基因及其蛋白质产物的服务，并且通过使用这一点，一种用于生产饲料添加剂，洗涤剂组合物和生物燃料的方法。

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