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公开(公告)号：KR1020040041475A
公开(公告)日：2004-05-17
申请号：KR1020020070532
申请日：2002-11-11
Applicant: 박근형
IPC: A23L2/04
Abstract: PURPOSE: An enzyme treated welsh onion extract composition containing a natural antioxidant active material obtained by enzymatic treatment of Welsh onion(Allium fistulosum) and then hot air drying or freeze drying thereof is provided. It can be effectively used as an antioxidant functional material having disease preventing and aging inhibiting function. CONSTITUTION: A Welsh onion extract is subjected to an enzymatic treatment with Celluclast(Novo Co.) and Viscozyme(Novo Co.) and then hot air-dried or freeze-dried. The Welsh onion extract composition contains an antioxidant material such as 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, 3,4-dihydroxycinnamic acid having antioxidant activity.
Abstract translation: 目的：提供含有通过酶处理威尔士葱（葱属葱）获得的天然抗氧化活性物质，然后热空气干燥或冷冻干燥的酶处理的威尔士洋葱提取物组合物。 它可以有效地用作具有预防疾病和衰老抑制功能的抗氧化功能材料。 构成：将威尔士洋葱提取物用Celluclast（Novo Co.）和Viscozyme（Novo Co.）进行酶处理，然后热空气干燥或冷冻干燥。 威尔士洋葱提取物组合物含有抗氧化剂，如4-羟基苯甲酸，4-羟基-3-甲氧基苯甲酸，3,4-二羟基苯甲酸，3,4,5-三羟基苯甲酸，具有抗氧化活性的3,4-二羟基肉桂酸 。
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62.发明公开항산화 활성을 갖는 4-하이드록시벤조산,4-하이드록시-3-메톡시벤조산, 3,4-디하이드록시벤조산,3,4,5-트리하이드록시벤조산, 3,4-디하이드록시시나믹산등의 천연 항산화 활성물질을 포함한 항산화 기능을 갖는복분자착즙조제물 및 제조방법 无效含有4-羟基苯甲酸，4-羟基-3-甲氧基苯甲酸，3,4-二羟基苯甲酸，3,4,5-三羟基苯甲酸和3,4-二羟基琥珀酸的抗氧化功能的RUBUS COREANUM果汁组合物及其制备方法
公开(公告)号：KR1020040019806A
公开(公告)日：2004-03-06
申请号：KR1020020052643
申请日：2002-08-30
Applicant: 박근형
Abstract: PURPOSE: Provided are a Rubus coreanum fruit juice composition which has antioxidative function and contains, as natural antioxidants, 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, and 3,4-dihydroxycinnamic acid and a preparation method thereof. CONSTITUTION: A Rubus coreanum fruit juice composition having antioxidative function is characterized by containing, as natural antioxidative matters, 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, and 3,4-dihydroxycinnamic acid which have radical scavenging activity and antioxidative function.
Abstract translation: 目的：提供具有抗氧化功能的Rubus coreanum果汁组合物，作为天然抗氧化剂含有4-羟基苯甲酸，4-羟基-3-甲氧基苯甲酸，3,4-二羟基苯甲酸，3,4,5-三羟基苯甲酸 和3,4-二羟基肉桂酸及其制备方法。 构成：具有抗氧化功能的芦荟果汁组合物的特征在于含有作为天然抗氧化剂的4-羟基苯甲酸，4-羟基-3-甲氧基苯甲酸，3,4-二羟基苯甲酸，3,4,5-三羟基苯甲酸 ，和具有自由基清除活性和抗氧化功能的3,4-二羟基肉桂酸。
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公开(公告)号：KR100388357B1
公开(公告)日：2003-06-19
申请号：KR1020000072613
申请日：2000-12-01
Applicant: 박근형
IPC: C07D213/62
Abstract: PURPOSE: A novel natural antimicrobial substance isolated from the stalk and leaf of Hovenia dulcis Thunb and an isolating method thereof are provided. Therefore, the novel natural antimicrobial substance, hovenidine B6, having pyridine structure can be found. CONSTITUTION: The novel natural antimicrobial substance, hovenidine B6 is isolated from the stalk and leaf of Hovenia dulcis Thunb by the steps of: heat-extracting the stalk and leaf of Hovenia dulcis Thunb; centrifuging the extract and freeze-drying the supernatant to obtain the extract powder; concentrating the methanol filtrate under reduced pressure to obtain methanol extract; adding a weak alkali buffer and ethylacetate into the methanol extract to divide a liquid fraction and an ethylacetate neutral fraction, and adding ethylacetate into the liquid fraction to obtain ethylacetate acid fraction; subjecting the above fractions to silica gel adsorption column chromatography using ethylacetate-methanol solution to obtain an active fraction; subjecting the active fraction to gel filtration chromatography using Sephadex LH-20 column; and subjecting the purified fraction to reverse HPLC using methanol-water solvent to isolate hovenidine B6.
Abstract translation: 用途：提供一种从枳st leaf和叶中分离出的新型天然抗菌物质及其分离方法。 因此，可以发现具有吡啶结构的新型天然抗微生物物质hovenidine B6。 本发明的新颖天然抗菌物质Hovenidine B6是通过以下步骤从枳st st和叶中分离出来的：热榨枳st st和叶; 离心提取物并冷冻干燥上清液以获得提取物粉末; 减压浓缩甲醇滤液得到甲醇提取物; 向甲醇提取液中加入弱碱缓冲液和乙酸乙酯，分液体部分和乙酸乙酯中性部分，向液体部分中加入乙酸乙酯，得到乙酸乙酯酸部分; 使用乙酸乙酯 - 甲醇溶液将上述级分进行硅胶吸附柱色谱法以获得活性级分; 用Sephadex LH-20柱对活性部分进行凝胶过滤层析; 并使用甲醇 - 水溶剂使纯化级分进行反相HPLC以分离Hovenidine B6。
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公开(公告)号：KR1020010090122A
公开(公告)日：2001-10-18
申请号：KR1020000014753
申请日：2000-03-23
Applicant: 박근형
IPC: C07C50/02
Abstract: PURPOSE: A novel natural antibacterial compound separated from the root of Pulsatilla Koreana Nakai and its separation method are provided, wherein the compound is named after pulsaquinone and can be used as a lead compound for preparing the various active material. CONSTITUTION: The antibacterial compound named pulsaquinone is represented by the formula 1. The separation method comprises the steps of: vacuum-evaporating the methanol extract of the dry root of Pulsatilla Koreana Nakai to obtain the extract to collect the aqueous part; partitioning the extract with weak alkali buffer and ethyl acetate; controlling the aqueous part to be weak acidic; partitioning the aqueous part with ethyl acetate; eluting the active partition from the ethyl acetate extract by the silica gel adsorption chromatography with ethyl acetate and methanol with increasing the content of methanol gradually; eluting the active material from the active partition by the silica gel adsorption chromatography with ethyl acetate and chloroform; and obtaining pulsaquinone from the active material by carrying out the Sephadex LH-20 chromatography, the silica gel partition column chromatography, the reverse HPLC using methanol-water gradient system and the reverse HPLC in order.
Abstract translation: 目的：提供从白头翁根中分离的新型天然抗菌化合物及其分离方法，其中化合物以脉络嗪命名，可用作制备各种活性物质的铅化合物。 构成：称为脉络洛芬的抗菌化合物由式1表示。分离方法包括以下步骤：真空蒸发白芥白粉干根的甲醇提取物，得到提取物收集含水部分; 用弱碱缓冲液和乙酸乙酯分配提取物; 控制水性部分为弱酸性; 用乙酸乙酯分配水性部分; 用乙酸乙酯和甲醇通过硅胶吸附色谱法从乙酸乙酯萃取液中洗脱活性分配物，逐渐增加甲醇含量; 通过硅胶吸附色谱法用乙酸乙酯和氯仿从活性隔板中洗脱活性物质; 通过进行Sephadex LH-20色谱，硅胶分配柱层析，反相HPLC，使用甲醇 - 水梯度系统和反相HPLC，从活性物质中获得脉冲醌。
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Patent Agency Ranking
公开(公告)号：KR1020010083531A
公开(公告)日：2001-09-01
申请号：KR1020000007233
申请日：2000-02-16
Applicant: 박근형
IPC: A47K7/00
Abstract: PURPOSE: A tea leaf catechin contained wet tissue producing method is provided to protect a skin from aging and inflammation by adding catechins of tea leaves with antibiosis and acid resistance. CONSTITUTION: A wet tissue comprises an unwoven fabric or pulp with purified water, a keratin remover, a surfactant, a moisturizer, a perfume and a pH control agent. Herein, 0.5 to 2% of catechins of tea leaves is added to the soluble additions. 40ml of the soluble additions is included per 10 pieces of wet tissues so that 20mg to 80mg of catechins is included per wet tissue. Therefore, when using the wet tissue, a user expects a skin to be protected from aging and inflammation with the antibiosis and acid resistance of the catechins.
Abstract translation: 目的：提供一种含茶叶儿茶素的湿纸巾生产方法，通过加入具有抗生素和耐酸性的茶叶儿茶素，保护皮肤免于衰老和炎症。 构成：湿纸巾包括无纺织物或纸浆与净化水，角蛋白去除剂，表面活性剂，保湿剂，香料和pH控制剂。 这里，将0.5〜2％的茶叶儿茶素加入到可溶性添加剂中。 每10个湿纸巾包含40ml的可溶性添加剂，每个湿纸巾包含20mg至80mg的儿茶素类。 因此，当使用湿纸巾时，使用者期望保护皮肤免受老化和炎症的反抗和儿茶素的耐酸性。
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公开(公告)号：KR100270712B1
公开(公告)日：2000-11-01
申请号：KR1019960046565
申请日：1996-10-17
Applicant: 박근형
Abstract: PURPOSE: A method for extracting benzoic acid having a strong effect on inhibiting the growth of a microorganism from a pine needle and a microorganism growth inhibiting agent composition containing the pine needle extracts are provided. Whereby, the obtained composition has low toxicity while having sterilizing and preservative action. CONSTITUTION: This extraction method of benzoic acid comprises the steps of: washing a pine needle with hexane and extracting with ethyl acetate; obtaining an ethyl acetate soluble acidic fraction by distributing the ethyl acetate extracts into ethyl acetate and a phosphoric acid buffer solution; fractioning the ethyl acetate soluble acidic fraction into hexane and an ethyl acetate mixed solvent; and performing silica absorption chromatography and Cephadex LH-20 column chromatography of hexane-ethyl acetate soluble fraction.
Abstract translation: 目的：提供一种提取具有抑制松针生长微生物的强烈作用的苯甲酸的方法和含有松针提取物的微生物生长抑制剂组合物。 由此得到的组合物具有低毒性，同时具有消毒和防腐作用。 构成：该苯甲酸的提取方法包括以下步骤：用己烷洗涤松针并用乙酸乙酯萃取; 通过将乙酸乙酯萃取液分配到乙酸乙酯和磷酸缓冲溶液中得到乙酸乙酯可溶性酸性部分; 将乙酸乙酯可溶性酸性部分分馏成己烷和乙酸乙酯混合溶剂; 并进行己烷 - 乙酸乙酯可溶级分的二氧化硅吸收色谱和Cephadex LH-20柱色谱。
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公开(公告)号：KR100213487B1
公开(公告)日：1999-08-02
申请号：KR1019970030878
申请日：1997-07-03
Applicant: 박근형
IPC: C12P1/00
Abstract: 본 발명은 청록색계 색소의 제조방법에 관한 것으로, 좀더 상세하게는 치자열매로 부터 조제된 치자황색소를 미생물의 배지에 첨가 배양하여 청록계 색소를 제조하는 방법에 관한 것이다.
본 발명의 목적은 미생물의 변환능력을 이용하여 치자 황색소로 부터 청록계의 색소를 제조하는 방법을 제공함에 있다.
즉, 본 발명은 스트렙토코거스속(Streptococcus sp.), 스타필로코커스속(Staphylococcus sp.), 락토바실러스속(Lactobacillus sp.), 비브리오 속(Vibrio sp.), 살모넬라속(Salmonella sp.), 슈도모나스 속 (Peudomonas sp.) 또는 바실루스속(Bacillus sp.)에 속하는 미생물배지에 치자황색소 추출물을 첨가 배양하여 청녹색계 색소를 제조하는 방법임을 특징으로 한다.
본 발명에 의하면 미생물에 의해 치자황색소가 청녹색계색소로 변환되어 청녹색계 색소를 제조할 수 있으며, 치자황색소의 청녹색계색소로의 변환은 미생물에 따라 변환능을 갖는 미생물과 변환능이 없는 미생물 로 나뉘어지고, 변환능이 있는 미생물도 균주에 따라 변환양상의 차이를 나타내어 미생물이 갖는 변환능력의 유무와 변환양상의 차이는 미생물의 식별 및 확인방법으로 이용될 수 있다.-
公开(公告)号：KR100196825B1
公开(公告)日：1999-06-15
申请号：KR1019960027725
申请日：1996-07-10
Applicant: 박근형
Abstract: 본 발명은 밤나무 잎을 원료로 한 차(茶) 및 음료의 제조방법에 관한 것으로, 밤나무 잎으로부터 중열 또는 발효, 풍냉, 건조, 분쇄, 미분말화 등의 과정을 거쳐 밤나무 잎으로 된 증제차, 중제유념차, 증제가루차, 발효유념차, 반발효유념차 등을 제조할 수 있으며, 이렇게 제조된 차의 열수 추출액을 음료화함으로써 밤나무 잎을 유용하게 이용할 수 있다.
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公开(公告)号：KR1019980030419A
公开(公告)日：1998-07-25
申请号：KR1019960049812
申请日：1996-10-29
Applicant: 박근형
IPC: A23B7/10
Abstract: 본 발명은 차엽의 카테킨을 포함하는 김치 산패 억제제 및 그의 제조 방법에 관한 것으로, 차엽을 열수로 추출하고, 추출액을 클로로포름으로 세척하고, 물 분획을 에틸아세테이트로 분배시킨 후 에틸아세테이트 분획을 농축하여 유기 용매를 제거하고, 농축물을 물에 녹여 동결건조시키는 단계를 포함하는 방법에 의해 제조된 카테킨은 김치에 첨가시 김치 발효 관련 미생물, 특히 김치산패 관련 미생물의 생육을 억제하여 김치의 산패를 효과적으로 억제할 수 있다.
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