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公开(公告)号：KR1020100066652A
公开(公告)日：2010-06-18
申请号：KR1020080125050
申请日：2008-12-10
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A medium for selective culture of brucella is provided to selectively culture and isolate brucella in a short time and to research in infection analysis and dynamic study. CONSTITUTION: A medium for selective culture of brucella contains 0.01-1.0 weight% of erythritol as a carbon source. The medium additionally contains neutral red. The medium is tryptic soy agar in which dextrose is removed. The medium also contains: bacitracin or vancomycin as an antibacterial agent to gram-positive bacteria; azotrenam or nalidixic acid as an antibacterial agent to gram-negative bacteria; or amphotericine B or nystatin as an antifungal agent.
Abstract translation: 目的：提供选择性培养布鲁氏菌的培养基，以便在短时间内选择性培养和分离布鲁杆菌，并进行感染分析和动态研究。 构成：用于选择性培养布鲁氏菌的培养基含有0.01-1.0重量％的赤藓糖醇作为碳源。 该介质还含有中性红。 培养基是去除葡萄糖的胰蛋白酶大豆琼脂。 培养基还含有：杆菌肽或万古霉素作为革兰氏阳性菌的抗菌剂; azotrenam或萘啶酸作为革兰氏阴性细菌的抗菌剂; 或两性霉素B或制霉菌素作为抗真菌剂。
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公开(公告)号：KR100951056B1
公开(公告)日：2010-04-07
申请号：KR1020070109076
申请日：2007-10-29
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 라텍스 비드를 이용한 네오스포라병의 진단 시약 및 진단 키트에 관한 것이다. 보다 구체적으로 본 발명은 네오스포라 캐니눔(
Neospora
caninum ) 항원으로 감작시킨 라텍스 비드를 포함하는 네오스포라병 진단 시약, 이를 포함하는 네오스포라병 진단 키트 및 이를 이용한 네오스포라병 진단 방법에 관한 것이다.
본 발명에 따른 네오스포라 원충 항원 및 라텍스 비드를 이용한 진단 시약은 네오스포라병에 대한 정확한 진단을 고가의 장비없이 간편하게 수행할 수 있는 효과가 있다.
네오스포라병, 라텍스 비드, 진단-
公开(公告)号：KR1020090088169A
公开(公告)日：2009-08-19
申请号：KR1020080013557
申请日：2008-02-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , (주)래피젠
IPC: G01N33/50 , G01N30/02 , G01N33/68 , G01N33/543
Abstract: A diagnosis kit of neosporosis using immunochromatography is provided to simply and economically diagnose neosporosis without expensive equipment. A diagnosis kit using immunochromatography comprises a neospora canium antigen which is adhered on a film. The antigen is Neospora caninum Kr2 strain. The film is a nitrocellulose film. The diagnosis further comprises anti-bovine immunoglobulin G on the film.
Abstract translation: 提供使用免疫色谱法的新孢子虫病诊断试剂盒，用于简单且经济地诊断新孢子虫病，无需昂贵的设备。 使用免疫色谱法的诊断试剂盒包括粘附在膜上的新孢子虫抗原。 抗原是新孢子虫Kr2株。 该膜是硝酸纤维素膜。 该诊断还包括抗牛免疫球蛋白G。
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公开(公告)号：KR1020090028213A
公开(公告)日：2009-03-18
申请号：KR1020070093644
申请日：2007-09-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K36/344 , A61P37/02
Abstract: A manufacturing method of extract of codonopsis lanceolata is provided to increase immunomodulation enhancement of pathogenic bacteria, to be useful as immunostimulator preventing and treating efficiently disease of the animal, an adjuvant improving effect of vaccine and an adjuvant treatment making therapeutic efficacy reinforced. A manufacturing method of extract of codonopsis lanceolata comprises steps of: obtaining the first extracts and residue by heating powder of leaf and stem of the dried Condonopsis lanceolata Trautv into distilled water, separating the first extracts separately, putting it into residue, heating it obtaining the second extraction extracts; mixing the first extracts of the obtained Condonopsis lanceolata Trautv stem and leaf and the second extracts of Condonopsis lanceolata Trautv stem and leaf, putting ethanol into the mixing extract, obtaining precipitated polysaccharide by stationing the obtained solution and centrifuging the precipitated polysaccharide; and center-rinsing the separated polysaccharide to the ethanol, drying the washed material, dissolving dried material with distilled water, filtering the obtained solution and obtaining undiluted solution.
Abstract translation: 提供密码子提取物的制造方法，以增加病原菌的免疫调节增强作用，有效地用于预防和治疗动物有效疾病的免疫刺激剂，疫苗的辅助改善效果和辅助治疗，增强治疗功效。 密码子提取物的制造方法包括以下步骤：通过将干燥的马铃薯干燥杆菌的叶和茎的粉末加热到蒸馏水中获得第一提取物和残留物，分离第一提取物，将其放入残渣中，加热得到 第二提取提取物; 混合所得到的Condonopsis lanceolata Trautv茎和叶的第一提取物和Condonopsis lanceolata Trautv茎和叶的第二提取物，将乙醇加入到混合提取物中，通过沉淀所得溶液并离心沉淀的多糖得到沉淀的多糖; 将分离的多糖中心漂洗至乙醇，干燥洗涤过的物质，用蒸馏水溶解干燥物，过滤得到的溶液，得到未稀释溶液。
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公开(公告)号：KR1020090028212A
公开(公告)日：2009-03-18
申请号：KR1020070093643
申请日：2007-09-14
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K35/648
CPC classification number: A61K35/648
Abstract: A manufacturing method of Scolopendra subspinipes extract making immunogenicity of animal is provided to be useful as immunostimulator preventing and treating efficiently disease of the animal, an adjuvant improving effect of vaccine and an adjuvant treatment making therapeutic efficacy reinforced. A manufacturing method of Scolopendra subspinipes extract making immunogenicity of animal comprises steps of: put the Scolopendra subspinipes powder into distilled water; obtaining extract by heating obtained solution, filtering obtained extract using filter paper firstly, filtering filtered material using filter paper secondly and sterilizing filtered material.
Abstract translation: 提供了提供动物免疫原性的Scolopendra subspinipes提取物的制造方法，可用作有效预防和治疗动物疾病的免疫刺激剂，疫苗的辅助改善效果和辅助治疗，增强治疗功效。 Scolopendra subspinipes提取物制备动物免疫原性的方法包括以下步骤：将Scolopendra subspinipes粉末放入蒸馏水中; 通过加热获得的溶液获得提取物，首先使用滤纸过滤获得的提取物，然后用滤纸过滤过滤材料并对过滤材料进行灭菌。
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Patent Agency Ranking
公开(公告)号：KR1020090013600A
公开(公告)日：2009-02-05
申请号：KR1020070077855
申请日：2007-08-02
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: A61K36/06
Abstract: An immunostimulant containing the lystic extract of Candida sake is provided to increase activity of neutrophil and lymphocyte as major immune cells of animals, thereby protecting animals from diseases. The immunostimulant contains the lystic extract of Candida sake which is prepared by cultivating Candida sake in yeast malt extract agar broth at 30 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Candida sake suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.
Abstract translation: 提供含有念珠菌精液提取物的免疫刺激剂，以增加嗜中性粒细胞和淋巴细胞作为动物主要免疫细胞的活性，从而保护动物免受疾病。 免疫刺激剂含有通过在30℃下在酵母麦芽提取物琼脂培养液中培育念珠菌精油而制备的念珠菌清酒。 培养72小时，将培养液以3000Xg离心30分钟并收集，用pH7.2的磷酸盐缓冲盐水（PBS）冲洗收集的沉淀物，并将漂洗的沉淀悬浮于PBS中，加入5％含有50mM Tris -HCl，0.15M NaCl和4％Triton x-114作为增溶剂加入到念珠菌悬浮液中，在室温下搅拌混合物2-3天以溶解链霉菌，并将溶液的pH调节至6.5-7.2 ，并干燥恒温室中的溶液。
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公开(公告)号：KR1020090012575A
公开(公告)日：2009-02-04
申请号：KR1020070076507
申请日：2007-07-30
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K31/70 , A61K36/48 , A61K36/185 , A61P37/02
CPC classification number: A61K38/168 , Y10S514/885
Abstract: Glycine max (L.) Merrill lectin is provided to reinforce immunogenicity of animals, to improve effect of an immunoadjuvant and a vaccine preventing and treating disease of the animals efficiently and to increase defence ability aspecific to attack inoculation of pathogenic bacteria. An immunoadjuvant comprises Glycine max (L.) Merrill lectin as an active ingredient. Additionally, the Glycine max (L.) Merrill lectin is used for a vaccine throne and an adjuvant treatment as the active ingredient.
Abstract translation: 提供大豆（L.）美林凝集素以增强动物的免疫原性，提高免疫佐剂的效果和预防和治疗动物疾病的疫苗，并提高对病原菌接种的防御能力。 免疫佐剂包含甘氨酸（L.）美林凝集素作为活性成分。 另外，甘氨酸（L.）美林凝集素用作疫苗王位和辅助治疗作为活性成分。
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公开(公告)号：KR1020080095988A
公开(公告)日：2008-10-30
申请号：KR1020070040731
申请日：2007-04-26
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A feed additive using a coptic rhizome and forsythia fruit extract is provided to accelerate the growth of animal, to reinforce an immune function and to prevent diseases, by adding predetermined amount of extracts obtained from Forsythiae Fructus and Coptidis Rhizoma and supplying it to domestic animals, thereby substituting antibiotics. A feed additive using a coptic rhizome and forsythia fruit extract comprises Coptis japonica extract 50 weight%, forsythia extract 10 weight%, and dextrose 30 weight% and Glycyrrhiza extract 10 weight%.
Abstract translation: 提供使用科普特根和连翘果提取物的饲料添加剂，通过加入预定量的连翘和黄连提取物提取动物，增加免疫功能，增强免疫功能，预防疾病，并将其提供给家畜， 从而代替抗生素。 使用科普特根和连翘果提取物的饲料添加剂包括50重量％的黄连提取物，10重量％的连翘提取物和30重量％的葡萄糖和10重量％的甘草提取物。
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公开(公告)号：KR1020080032809A
公开(公告)日：2008-04-16
申请号：KR1020060098780
申请日：2006-10-11
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
CPC classification number: Y02A50/473 , A61K35/74 , A61K36/06 , C12N1/16 , C12N1/20
Abstract: A method for preparing a cell lysis composition of bacterial and yeast cell wall is provided to improve yield of cell wall, and selectively employ antigen substances and immune-enhancing substances in the cell wall by using biochemical and chemical treatment, so that the composition is useful as vaccine antigen, heterologous delivery system, immune enhancer, vaccine assistant and complex feed additive. A method for preparing a cell lysis composition of bacterial and yeast cell wall comprises the steps of: culturing gram positive bacteria, gram negative bacteria and yeast; treating the cultured gram positive bacteria, gram negative bacteria and yeast biochemically and chemically by using transparent outer membrane extraction, TNT(Tris-HCl, sodium chloride, Triton X-114) method, NaOH-EDTA(sodium hydroxide-(ethylene dinitrilo) tetraacetic acid), TMC(Tris-HCl, magnesium chloride, calcium chloride) method or TET(Tris-HCl, EDTA, Triton X-100) method; and extracting useful ingredients of cell wall from the treated gram positive bacteria, gram negative bacteria and yeast, wherein the gram negative bacteria include Escherichia coli 055 and Bordetella pertussis; the gram positive bacteria include Staphylococcus aureus Cowan 1 and Erysipelothrix rhusiopathiae T2; and the yeast is Saccharomyces cerevisiae or Candida albicans. Further, the useful ingredients of cell wall are selected from a group consisting of a transparent cell outer membrane, a cell wall and bacteriolysant.
Abstract translation: 提供细菌和酵母细胞壁细胞裂解组合物的制备方法，以提高细胞壁的产量，并通过生物化学和化学处理选择性地在细胞壁中使用抗原物质和免疫增强物质，使组合物有用 作为疫苗抗原，异源递送系统，免疫增强剂，疫苗助剂和复合饲料添加剂。 制备细菌和酵母细胞壁的细胞裂解组合物的方法包括以下步骤：培养革兰氏阳性菌，革兰氏阴性细菌和酵母; 通过使用透明的外膜提取，TNT（Tris-HCl，氯化钠，Triton X-114）方法，NaOH-EDTA（氢氧化钠 - （亚乙基二硝基）四乙酸）处理培养的革兰氏阳性细菌，革兰氏阴性细菌和酵母生物化学和化学 酸），TMC（Tris-HCl，氯化镁，氯化钙）法或TET（Tris-HCl，EDTA，Triton X-100）法; 并从经处理的革兰氏阳性细菌，革兰氏阴性细菌和酵母提取细胞壁的有用成分，其中革兰氏阴性细菌包括大肠杆菌055和百日咳博德特氏菌; 革兰氏阳性细菌包括金黄色葡萄球菌Cowan 1和Erysipelothrix rhusiopathiae T2; 酵母是酿酒酵母或白色念珠菌。 此外，细胞壁的有用成分选自透明细胞外膜，细胞壁和细菌悬液。
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70.发明授权동물혈청을 첨가하지 않은 돈단독균 배지 및 그 배지에서 배양한 돈단독균으로 제조되는 생균 및 사균백신 有权不含动物血清和猪丹毒的白纹丝杆菌血管病毒培养基用在该培养基上培养的细菌制备的活的和灭活的疫苗
公开(公告)号：KR100769356B1
公开(公告)日：2007-11-01
申请号：KR1020060007212
申请日：2006-01-24
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명자는 돈단독균의 발육능을 향상시키기 위하여 개발한 배지에서의 돈단독균 발육성과 이 배지에서 배양한 돈단독균으로 만든 불활화백신의 마우스에서의 면역원성을 시험하여 다음과 같은 결과를 얻었다.
돈단독 생균수는 기존의 브레인 하트 인퓨전(Brain Heart Infusion, BHI) 배지에 5-10% 소혈청을 첨가하여 배양하는 것보다 약 10배 이상의 균수 향상을 보였다.
37℃에서 18-20시간 배양후 배양배지의 pH가 7.0으로 매우 안정적인 pH를 나타내었다.
상기의 개발 배지에서 배양한 돈단독균으로 제조한 돈단독불활화 백신의 마우스에서의 항체형성능은 양호하였다.
돈단독 배양배지, 동물혈청 무첨가
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