公开(公告)号：WO2017083609A1

公开(公告)日：2017-05-18

申请号：PCT/US2016/061476

申请日：2016-11-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： BULLOCH, Kyle ,  DILLER, Thomas

IPC: G01N27/447

CPC classification number: G01N27/44747 ,  G01N27/44704 ,  G01N27/44782

Abstract: An apparatus (200) for preparation of an electrophoresis slab gel may include a base (220) having an opening configured to receive a cassette (100) configured to contain an electrophoresis slab gel, a clamping mechanism (240) configured to move relative to the base (220) between an open position in which the clamping mechanism (240) permits insertion of a cassette into the base (220), and a closed position in which the clamping mechanism (240) is configured to clamp a cassette received in the base, a compressible pad (260) operatively coupled to the clamping mechanism in a position to compress against a cassette (100) received in the base (220) in the closed position of the clamping mechanism (240). The cassette (100) may include a first plate (11) and a second plate (12), and a spacer mechanism (13) separate from each of the first and second plates, the spacer mechanism configured to be positioned between the inner faces of the first plate and the second plate. When subjected to a clamping force exerted on the outer faces of the first and second plates, the spacer mechanism is configured to maintain a separation distance between the inner faces of the first and second plates, and provide a seal to prevent leakage of an electrophoresis gel solution introduced between the plates.

Abstract translation: 用于制备电泳平板凝胶的装置（200）可以包括：具有开口的基座（220），所述开口构造成接收配置为容纳电泳平板凝胶的盒（100）;夹紧机构（ 240），所述闭合位置构造成相对于所述基座（220）在所述夹紧机构（240）允许将盒子插入所述基座（220）的打开位置与所述夹紧机构（240）配置于其中的闭合位置之间移动 以夹紧容纳在底座中的盒子;在夹紧机构（240）的关闭位置中，可操作地联接到夹紧机构的可压缩垫（260）压靠容纳在基座（220）中的盒子（100） 。 盒（100）可包括第一板（11）和第二板（12）以及与第一板和第二板中的每一个分离的间隔机构（13），间隔机构构造成定位在第一板 第一板和第二板。 当受到施加在第一和第二板的外表面上的夹紧力时，间隔机构被构造成保持第一和第二板的内表面之间的分离距离，并且提供密封以防止电泳凝胶的泄漏 解决方案介绍之间的板块。

公开(公告)号：WO2017069781A1

公开(公告)日：2017-04-27

申请号：PCT/US2015/057217

申请日：2015-10-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MARTIN, Philip

IPC: C12N1/06 ,  C12N15/10 ,  C12M1/00 ,  B01D61/14

CPC classification number: C12N1/06 ,  B01D61/147 ,  C12M47/04 ,  C12M47/06 ,  C12N15/1017

Abstract: A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.

Abstract translation: 涉及细胞悬液的细胞溶解工作流程，所述细胞悬液沿第一流动方向从滤器的第一侧朝向其第二侧穿过过滤器。 过滤器捕获第一侧上的多个细胞。 裂解溶液沿与第一方向相反的第二流动方向通过过滤器，由此移除捕集在过滤器上的多个细胞，并将多个细胞重新悬浮在过滤器的第一侧上的裂解溶液中。 裂解溶液裂解多个细胞以产生细胞裂解物。 细胞裂解物以第一流动方向通过过滤器。

公开(公告)号：WO2017027620A1

公开(公告)日：2017-02-16

申请号：PCT/US2016/046399

申请日：2016-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： COLBURN, Joel ,  RATH, Mousumi ,  GHANBARI, Sarah

IPC: B01L3/00 ,  G01N1/28

CPC classification number: G01N1/04 ,  B01L3/50825 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0838 ,  C12Q1/6806 ,  G01N1/2813 ,  G01N1/286 ,  G01N1/34 ,  G01N2001/028 ,  G01N2001/045 ,  G01N2001/284 ,  G01N2001/2886 ,  C12Q2521/327 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2531/113 ,  C12Q2565/601 ,  C12Q2535/122

Abstract: A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. The substrate includes an upper portion, a lower portion, and an intermediate portion disposed between the upper portion and the lower portion. The lower portion is disposed below the upper portion and comprises a bottom surface configured to receive a biological sample. The intermediate portion is characterized by a first substrate diameter and the lower portion is characterized by a second substrate diameter that is less than the first substrate diameter.

Abstract translation: 用于保持生物样品的载体包括基底。 衬底被构造成接合第一样品室，所述第一样品室包括由第一开口直径或第二样品室表征的第一开口，所述第一开口包括第二开口，第二开口的特征在于第二开口直径大于第一开口直径。 基板包括上部，下部和设置在上部和下部之间的中间部。 下部设置在上部下方，并且包括构造成接纳生物样品的底面。 中间部分的特征在于第一基底直径，下部的特征在于第二基底直径小于第一基底直径。

公开(公告)号：WO2016209753A1

公开(公告)日：2016-12-29

申请号：PCT/US2016/038305

申请日：2016-06-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MELQUIST, Penny ,  GEE, Kyle

IPC: G01N33/50 ,  G01N33/58 ,  C07D311/16 ,  C07D311/54 ,  C07F9/655 ,  C09B57/02

CPC classification number: G01N33/5005 ,  C09B57/02 ,  G01N33/582

Abstract: Described herein are compounds, methods, and kits for short- and long-term tracking of cell proliferation, differentiation, and/or function. The compounds disclosed herein are coumarin- based cell-tracking reagents that fluoresce in the blue portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, good retention within cells and low toxicity toward cells.

Abstract translation: 本文描述了用于短期和长期跟踪细胞增殖，分化和/或功能的化合物，方法和试剂盒。 本文公开的化合物是基于香豆素的细胞跟踪试剂，其在UV / VIS光谱的蓝色部分中发荧光，并提供明亮的荧光强度，均匀的细胞染色，细胞内的良好保留和对细胞的低毒性。

公开(公告)号：WO2016183478A1

公开(公告)日：2016-11-17

申请号：PCT/US2016/032442

申请日：2016-05-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KOLLER, Christian ,  ALLEN, Michael D.

IPC: G06F19/22

CPC classification number: C12Q1/6874 ,  G06F19/22

Abstract: Methods, system, and kits are provided for sample identification, and, more specifically, for designing, and/or making, and/or using sample discriminating codes or barcodes for identifying sample nucleic acids or other biomolecules or polymers. For example, a plurality of flowspace codewords may be generated, the codewords comprising a string of characters. A location for at least one padding character within the flowspace codewords may be determined. The padding character may be inserted into the flowspace codewords at the determined location. After the inserting, a plurality of the flowspace codewords may be selected based on satisfying a predetermined minimum distance criteria, wherein the selected codewords correspond to valid base space sequences according to a predetermined flow order. And the barcode sequences corresponding to the selected codewords may be manufactured.

Abstract translation: 提供方法，系统和试剂盒用于样品鉴定，更具体地说，用于设计和/或制作和/或使用用于鉴定样品核酸或其他生物分子或聚合物的样品鉴别代码或条形码。 例如，可以生成多个流空间码字，码字包括一串字符。 可以确定流行空间码字中的至少一个填充字符的位置。 填充字符可以被插入到所确定的位置处的流空间码字中。 在插入之后，可以基于满足预定的最小距离标准来选择多个流空间码字，其中所选择的码字根据预定的流顺序对应于有效的基本空间序列。 并且可以制造对应于所选择的码字的条形码序列。

公开(公告)号：WO2016172628A1

公开(公告)日：2016-10-27

申请号：PCT/US2016/029046

申请日：2016-04-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MAJUMDAR, Nivedita Sumi ,  BANERJEE, Swapnonil

IPC: G06F19/24

CPC classification number: C12Q1/6851 ,  G06F17/18 ,  G06F19/24

Abstract: A method for performing digital polymerase chain reaction (dPCR) is provided. The method comprises partitioning a biological sample volume including a plurality of target nucleic acids into a plurality of partitions. At least one partition includes at least one target nucleic acid. The method further includes determining a model for volume variation of the plurality of partitions and determining a fraction of partitions including at least one target nucleic acid. The method includes generating a concentration of target nucleic acids in the biological sample based on the model for volume variation and the fraction of partitions including at least one target nucleic acid.

Abstract translation: 提供了一种执行数字聚合酶链反应（dPCR）的方法。 该方法包括将包括多个靶核酸的生物样品体积分成多个分区。 至少一个分区包括至少一个靶核酸。 该方法还包括确定多个分区的体积变化的模型并确定包括至少一个靶核酸的分区的一部分。 该方法包括基于用于体积变化的模型和包括至少一种靶核酸的分区部分产生生物样品中靶核酸的浓度。

公开(公告)号：WO2016127090A1

公开(公告)日：2016-08-11

申请号：PCT/US2016/016832

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MARKS, Jeffrey

IPC: G01N21/27 ,  G01N21/64

CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2201/04 ,  G01N2201/068 ,  G01N2201/12746

Abstract: The present teachings relate to a method and system for normalizing spectra across multiple instruments. The method (800) comprises at least one reference instrument and a test instrument. Each instrument comprises at least one excitation filter and at least one emission filter arranged in pairs. Each instrument further comprises a pure dye plate comprising a plurality of wells. Each well contains a plurality of dyes where each dye comprises a fluorescent component. Fluorescent spectra are obtained from each instrument (805, 820) for each dye across multiple filter combinations to contribute to a pure dye matrix Mref for the reference instrument and pure dye matrix M for the test instrument. The pure dye spectra can then be multiplied by correction factors (840) for each filter pair to result in corrected pure dye spectra, then normalized (845) and the multicomponenting data can be extracted (850).

Abstract translation: 本教导涉及用于在多个仪器上归一化光谱的方法和系统。 方法（800）包括至少一个参考仪器和测试仪器。 每个仪器包括至少一个激励滤波器和成对布置的至少一个发射滤波器。 每个仪器还包括包含多个孔的纯染料板。 每个孔含有多种染料，其中每种染料包含荧光组分。 对于每种染料，通过多个过滤器组合从每个仪器（805,820）获得荧光光谱，以有助于参考仪器的纯染料矩阵Mref和测试仪器的纯染料矩阵M. 然后可以将纯染料光谱乘以每个过滤器对的校正因子（840），以产生校正的纯染料光谱，然后归一化（845），并且可以提取多组分数据（850）。

公开(公告)号：WO2016127032A1

公开(公告)日：2016-08-11

申请号：PCT/US2016/016730

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： WESSEL, Thomas ,  CHU, Yong ,  FREUDENTHAL, Jacob ,  WOO, David

IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68 ,  G06F19/26

CPC classification number: G01N21/6428 ,  G01N21/274 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G06F19/24

Abstract: A method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves (102, 202). The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves (104, 204) and determining, for each fluorescence threshold of the set, a first set of cycle threshold (C t ) values of amplification curves generated from the samples of the first quantity and a second set of C t values of amplification curves generated from the samples of the second quantity (106, 206). The method includes calculating if the first and second quantities are sufficiently distinguishable based on C t values at each of the plurality of fluorescence thresholds (108, 208-218).

Abstract translation: 提供了一种验证仪器的方法。 该方法包括从验证板接收放大数据以产生多个放大曲线（102,202）。 验证板包括第一数量和第二数量的样本，并且每个扩增曲线包括指数区域。 该方法还包括基于多个扩增曲线（104,204）的指数区域来确定一组荧光阈值，并且针对该组的每个荧光阈值确定放大曲线的第一组周期阈值（Ct）值 从第一数量的样本和从第二数量（106,206）的样本生成的第二组扩增曲线的Ctval值产生。 该方法包括基于多个荧光阈值（108,208-218）中的每一个的Ct值来计算第一和第二量是否足够可区分。

公开(公告)号：WO2016100895A1

公开(公告)日：2016-06-23

申请号：PCT/US2015/066836

申请日：2015-12-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MOZHAYSKIY, Vadim ,  FU, Yutao ,  HUBBELL, Earl

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1058 ,  C12Q1/6825 ,  C12Q2525/173 ,  C12Q2525/204 ,  C12Q2535/122 ,  C12Q2545/101 ,  C12Q2565/518 ,  C12Q2565/607

Abstract: A method for preparing a homopolymer recalibration panel includes: extracting, from a set of amplicons used in sequencing-by-synthesis, a set of candidate amplicons satisfying a first set of criteria, wherein the first set of criteria includes amplicons known to belong to high-confidence regions of a reference genome with no variants; and selecting, from the set of candidate amplicons, a reduced set of amplicons satisfying a second set of criteria, wherein the second set of criteria includes amplicons that together comprise at least a minimal threshold number of homopolymers of each homopolymer length between a predetermined minimal homopolymer length and a predetermined maximal homopolymer length for one or more of homopolymer types A, T, C, and G.

Abstract translation: 制备均聚物重校准面板的方法包括：从一组合成中使用的扩增子提取一组满足第一组标准的候选扩增子，其中第一组标准包括已知属于高分子的扩增子 没有变体的参考基因组的自信区域; 以及从所述候选扩增子集合中选择满足第二组标准的缩减的扩增子集，其中所述第二组标准包括一起包括至少最小阈值数量的预定最小均聚物之间的每个均聚物长度的均聚物的最小阈值数的扩增子 长度和一种或多种均聚物类型A，T，C和G的预定最大均聚物长度。

公开(公告)号：WO2016065249A1

公开(公告)日：2016-04-28

申请号：PCT/US2015/057095

申请日：2015-10-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： COLLINS, Scott P. ,  BRAU, Christopher D. ,  JONES, Nephi D.

IPC: B01D11/04 ,  G01N1/40 ,  B01D17/04

CPC classification number: B01D11/0423 ,  B01D11/043 ,  B01D11/0449 ,  B01D11/0457 ,  G01N1/34 ,  G01N2001/4061

Abstract: A sample purification system includes a container assembly bounding a sample purification compartment and having an upper end and an opposing lower end, the sample purification compartment comprising mixing zones and settling zones. A plurality of shielding elements are positioned within the sample purification compartment so as to at least partially separate adjacent mixing zones and settling zones or separate adjacent mixing zones, the mixing zones being in fluid communication with the settling zones. A mixing element is disposed within each mixing zone. An acoustic wave settler is aligned with a portion of the container assembly, the acoustic wave settler being configured to emit an acoustic wave through the portion of the container assembly and a mixture disposed therein, the acoustic wave coalescing fluid phase droplets disposed in the mixture to increase the buoyancy or density of the fluid phase droplets.

Abstract translation: 样品净化系统包括界定样品净化室并具有上端和相对的下端的容器组件，样品净化室包括混合区和沉降区。 多个屏蔽元件位于样品净化室内，以便至少部分地分离相邻的混合区域和沉降区域或分离相邻的混合区域，混合区域与沉降区域流体连通。 混合元件设置在每个混合区内。 声波沉降器与容器组件的一部分对准，声波沉降器被配置为通过容器组件的一部分和布置在其中的混合物发射声波，声波将布置在混合物中的流体相液滴聚结到 增加流体相液滴的浮力或密度。