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公开(公告)号：KR1020130054497A
公开(公告)日：2013-05-27
申请号：KR1020110117784
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting CPMMV(cowpea mild mottle virus) is provided to minimize damage due to viral diseases in crops and to enhance production. CONSTITUTION: An oilgonucleotide probe set for diagnosing or detecting CPMMV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 578-587 and complementary oligonucleotides thereof. A method for diagnosing or detecting CPMMV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA using the RNA as a template; a step of hybridizing the cDNA with the probe set; and a step of identifying the hybridization. [Reference numerals] (AA) Green pea; (BB) Host: Green pea
Abstract translation: 目的：提供用于诊断或检测CPMMV（cow豆轻度斑驳病毒）的探针，以尽量减少由于作物病毒性疾病引起的损伤，并提高产量。 构成：用于诊断或检测CPMMV的石油核苷酸探针组含有选自序列号578-587的寡核苷酸和其互补寡核苷酸的一种或多种寡核苷酸。 一种用于诊断或检测CPMMV的方法包括：从样品中分离RNA的步骤; 使用RNA作为模板合成cDNA的步骤; 将cDNA与探针组杂交的步骤; 以及鉴定杂交的步骤。 （附图标记）（AA）绿豆; （BB）主持人：绿豆
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公开(公告)号：KR1020130054496A
公开(公告)日：2013-05-27
申请号：KR1020110117760
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting PSV(peanut stunt virus) is provided to minimize damage due to viral diseases in crops and to enhance production. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting PSV contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 1025-1034, 1045-1049, and 2988, and complementary oligonucleotides thereof. A method for diagnosing or detecting PSV comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA; a step of hybridizing the cDNA with the probe set; and a step of detecting hybridization of the cDNA and the probe set. [Reference numerals] (AA) Cowpea; (BB) Host: cowpea; (CC) Kidney bean; (DD) Host: Kidney bean
Abstract translation: 目的：提供用于诊断或检测PSV（花生特技病毒）的探针，以尽量减少作物病毒性疾病引起的损伤，并提高产量。 构成：用于诊断或检测PSV的寡核苷酸探针组含有选自序列号1025-1034,1045-1049和2988的寡核苷酸及其互补寡核苷酸的一种或多种寡核苷酸。 用于诊断或检测PSV的方法包括：从样品中分离RNA的步骤; 合成cDNA的步骤; 将cDNA与探针组杂交的步骤; 以及检测cDNA和探针组杂交的步骤。 （AA）Cow豆; （BB）主持人：cow豆; （CC）芸豆; （DD）主机：芸豆
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公开(公告)号：KR1020130054494A
公开(公告)日：2013-05-27
申请号：KR1020110117752
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing or detecting BBWV2(Broad bean wilt virus2) is provided to minimize damage due to viral diseases in crops and to increase product yield. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting BBWV2 contains one or more oligonucleotides selected from the group consisting of oligonucleotides in sequence numbers 1143-1152 and 1158-1164 and complementary oligonucleotides thereof. A method for diagnosing or detecting BBWV2 comprises: a step of isolating RNA from a sample; a step of synthesizing cDNA using RNA as a template; a step of hybridizing the probe set with cDNA; and a step of detecting hybridization.
Abstract translation: 目的：提供用于诊断或检测BBWV2（蚕豆枯萎病毒2）的探针，以尽量减少作物病毒性疾病造成的损伤，并提高产品产量。 构成：用于诊断或检测BBWV2的寡核苷酸探针组含有选自序列号1143-1152和1158-1164的寡核苷酸及其互补寡核苷酸的一种或多种寡核苷酸。 一种用于诊断或检测BBWV2的方法包括：从样品中分离RNA的步骤; 使用RNA作为模板合成cDNA的步骤; 探针组与cDNA杂交的步骤; 和检测杂交的步骤。
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公开(公告)号：KR1020130054484A
公开(公告)日：2013-05-27
申请号：KR1020110117611
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/04 , C12Q1/6837
Abstract: PURPOSE: A probe set for diagnosing Genus Carmovirus is provided to minimize viral diseases in crops and to enhance product yields. CONSTITUTION: An oligonucleotide probe set for diagnosing or detecting Genus Carmovirus contains one or more oligonucleotides selected from a group consisting of oligonucleotides of sequence numbers 674, 682-685, 691-692, 730-734, 746-748, 1452-1458, 1665-1672, 2371, and 2745-2754, and complementary oligonucleotides thereof. A method for diagnosing or detecting Genus Carmovirus comprises: a step of isolating RNA from a sample which is suspected to have Genus Carmovirus infection; a step of synthesizing cDNA; a step of hybridizing the cDNA and the probe set; and a step of detecting hybridization. [Reference numerals] (AA) Jangyeobkong; (BB) Host: Jangyeobkong
Abstract translation: 目的：提供用于诊断卡门诺病毒属的探针，以尽量减少作物中的病毒性疾病，并提高产品产量。 构成：用于诊断或检测属Carmovirus属的寡核苷酸探针组含有一种或多种选自序列号674,682-685,691-692,730-734,746-748,1452-1458,1665的寡核苷酸的寡核苷酸 -1672,2731和2745-2754及其互补寡核苷酸。 用于诊断或检测属Carmovirus的方法包括：从怀疑具有Carmovirus类病毒感染的样品中分离RNA的步骤; 合成cDNA的步骤; cDNA和探针组杂交的步骤; 和检测杂交的步骤。 （附图标记）（AA）Jangyeobkong; （BB）主持人：Jangyeobkong
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公开(公告)号：KR1020130033076A
公开(公告)日：2013-04-03
申请号：KR1020110096920
申请日：2011-09-26
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/823 , A01H4/008 , C07K14/415 , C12N15/8205
Abstract: PURPOSE: A BrCYS7 promoter which is specific to receptable is provided to specifically induce gene expression in a certain tissue, and to effectively produce a gene product. CONSTITUTION: A promoter contains a nucleic acid sequence in sequence number 1. The promoter contains a nucleic acid sequence having 70% or more of sequence homology with the nucleic acid in sequence number 1. An expression vector contains the promoter, and is a pPBrCYS7 vector. A cell is transformed with the expression vector, and is an Agrobacterium sp. microorganism or a plant cell.
Abstract translation: 目的：提供特异性诱导的BrCYS7启动子，以特异性诱导特定组织中的基因表达，并有效产生基因产物。 构成：启动子含有序列号1中的核酸序列。启动子含有与序列号1中的核酸具有70％以上序列同源性的核酸序列。表达载体含有启动子，是pPBrCYS7载体 。 用表达载体转化细胞，是农杆菌属。 微生物或植物细胞。
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Patent Agency Ranking
公开(公告)号：KR1020100058827A
公开(公告)日：2010-06-04
申请号：KR1020080117379
申请日：2008-11-25
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A pollen-specific expression promoter is provided to reduce time and labor for inducing male sterility and to produce superior seeds. CONSTITUTION: A pollen-specific expression promoter PBrRF1 comprises a sequence of sequence number 1. A primer for amplifying the pollen-specific expression promoter PBrRF1 comprises oligonucleotides of sequence number 2 and sequence number 3. An expression vector pBGWFS7-PBrRF1 contains a promoter of sequence number 1. A method for preparing the expression vector pBGWFS7-PBrRF1 comprises: a step of isolating a promoter site of sequence number 1 from a Brassica campestris ssp. Pekinensis; a step of preparing a primer set of sequence numbers 2 and 3 from a genomic DNA isolated from the Arabidopsis thaliana; a step of isolating the promoter site through DNA amplification; a step of performing recombination of Pdonr221 vector and amplified PCR product to obtain a cloning vector of pDONR-PBrRF1 and pDONR-P35S; a step of performing LR recombination of pBGWFS7 vector containing a reporter system; and a step of transforming a plant body with the expression vector pBGWFS7-PBrRF1.
Abstract translation: 目的：提供花粉特异性表达启动子，以减少诱导雄性不育和产生优质种子的时间和劳动。 构成：花粉特异性表达启动子PBrRF1包含序列号1的序列。扩增花粉特异性表达启动子PBrRF1的引物包含序列号2和序列号3的寡核苷酸。表达载体pBGWFS7-PBrRF1含有序列的启动子 1。一种制备表达载体pBGWFS7-PBrRF1的方法，包括：分离序列号1的启动子位点与芸苔属芥菜的步骤。 大白菜; 从拟南芥分离的基因组DNA制备序列号2和3的引物组的步骤; 通过DNA扩增分离启动子位点的步骤; 进行Pdonr221载体和扩增的PCR产物重组的步骤，得到pDONR-PBrRF1和pDONR-P35S的克隆载体; 执行含有报告系统的pBGWFS7载体的LR重组的步骤; 以及用表达载体pBGWFS7-PBrRF1转化植物体的步骤。
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77.发明授权벼잎줄무늬병원균 또는 벼흰잎마름병원균 검출용 프라이머쌍 및 이를 이용한 멀티플렉스 ＰＣＲ 방법 有权用于检测黄单胞菌属（Xanthomonas oryzae pv。）的引物组。 oryzicola或黄单胞菌Xanthomonas oryzae pv。 米曲霉和多重PCR方法
公开(公告)号：KR100942390B1
公开(公告)日：2010-02-17
申请号：KR1020080008994
申请日：2008-01-29
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 벼잎줄무늬병원균 또는 벼흰잎마름병원균 검출용 프라이머 쌍 및 이를 이용한 멀티플렉스 PCR 방법에 관한 것으로, 본 발명의 프라이머 쌍은 벼잎줄무늬병원균 또는 벼흰잎마름병원균의 특이 서열과 관련되어, 신속, 정확하게 벼잎줄무늬병원균 또는 벼흰잎마름병원균을 검출하는데 유용하게 이용될 수 있으며, 또한 이를 이용한 멀티플렉스 PCR 방법은 식물체가 벼잎줄무늬병원균 및 벼흰잎마름병원균에 감염 되었는지 여부를 동시에 판별하는데 유용하게 이용될 수 있다.
벼잎줄무늬병원균, 벼흰잎마름병원균, 프라이머, 멀티플렉스-
公开(公告)号：KR1020060110880A
公开(公告)日：2006-10-26
申请号：KR1020050032626
申请日：2005-04-20
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/29
Abstract: A single expression genetic marker that is expressed according to the chromosomes of Brassica rapa genome is provided to discriminate chromosomes of Brassica rapa and quantitatively analyze the genetically modified crops of Brassica rapa. The single expression genetic marker that is expressed according to the chromosomes of Brassica rapa genome comprises 64 kinds of single expression genes located according to the related groups of Brassica rapa, having the nucleotide sequences of SEQ ID NO:1 to SEQ ID NO:64, wherein the single expression genetic marker comprises combinations of at least two genes selected from the 64 kinds of single expression genes of SEQ ID NO:1 to SEQ ID NO:64; and the single expression genes are identified by analyzing the distribution positions of single expression genes in the chromosomes of Brassica rapa.
Abstract translation: 提供根据芸苔属芸苔基因组染色体表达的单一表达遗传标记，以区分芸苔属的染色体，并定量分析芸苔属的转基因作物。 根据芸苔属芸苔基因组染色体表达的单表达遗传标记包含根据芸苔属相关组的64种单一表达基因，其具有SEQ ID NO：1至SEQ ID NO：64的核苷酸序列， 其中所述单一表达遗传标记包含选自SEQ ID NO：1至SEQ ID NO：64的64种单一表达基因的至少两种基因的组合; 并通过分析芸苔属染色体中单个表达基因的分布位置来鉴定单一表达基因。
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公开(公告)号：KR101869788B1
公开(公告)日：2018-06-21
申请号：KR1020160172791
申请日：2016-12-16
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N1/20 , A01N63/02 , C12P13/005 , C12R1/01
Abstract: 본발명은신규한류코노스톡시트레움 SY-3 균주및 이의용도에관한것으로, 더욱상세하게는생리작용을하는 GABA(gamma-aminobutyric acid, 감마아미노부티르산) 및 EPS(exopolysaccharide, 엑소폴리싸카라이드)의생성능을가지면서, 프로테아제(protease) 효소활성및 항균활성을갖는류코노스톡시트레움 SY-3 균주(KACC 81029BP), 상기균주또는이의배양액을유효성분으로포함하는미생물제제, 상기균주를배양하는단계를포함하는미생물제제의제조방법, 상기균주를포함하는발효식품, 상기균주를포함하는흰점박이꽃무지의진균병및 세균병방제용제제에관한것이다.
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公开(公告)号：KR101650560B1
公开(公告)日：2016-08-24
申请号：KR1020110117789
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 1307 내지 1311의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 LRSV (Lychnis ringspot virus) 올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 LRSV 진단또는검출용유전자칩, LRSV에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 LRSV를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 LRSV는검출용키트에관한것이다.
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