中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

155 records (took 0.024 seconds)

    PEF-TU expression units
    72.
    发明专利
    PEF-TU expression units 未知

    公开(公告)号：ZA200605863B

    公开(公告)日：2008-01-30

    申请号：ZA200605863

    申请日：2006-07-17

    Applicant: BASF AG

    Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

    IPC: C07K14/34 ,  C12N20090101 ,  C12N1/00 ,  C12N15/63 ,  C12N15/67 ,  C12N15/77 ,  C12P20090101 ,  C12P1/00 ,  C12P13/08 ,  C12P13/12

    Abstract: Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42).

    73.
    发明专利
    未知

    公开(公告)号：NO20062447L

    公开(公告)日：2006-09-14

    申请号：NO20062447

    申请日：2006-05-30

    Applicant: BASF AG

    Inventor： KROGER BURKHARD ,  ZELDER OSKAR ,  SCHRODER HARTWIG ,  HAFNER STEFAN ,  KIEFER PATRICK ,  WITTMANN CHRISTOPH ,  KLOPPROGGE CORINNA ,  HEINZLE ELMAR

    IPC: C12P13/08 ,  C12P20060101

    Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i. e., glycerol kinase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of glycerol kinase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

    75.
    发明专利
    未知

    公开(公告)号：NO20062683L

    公开(公告)日：2006-09-11

    申请号：NO20062683

    申请日：2006-06-09

    Applicant: BASF AG

    Inventor： KROGER BURKHARD ,  ZELDER OSKAR ,  SCHRODER HARTWIG ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN

    IPC: C07K14/34 ,  C12N20060101 ,  C12N1/00 ,  C12N1/21 ,  C12N15/63 ,  C12N15/67 ,  C12N15/77 ,  C12P1/00 ,  C12P13/08 ,  C12P13/12

    Abstract: Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42).

    77.
    发明专利
    未知

    公开(公告)号：DE102004061846A1

    公开(公告)日：2006-07-13

    申请号：DE102004061846

    申请日：2004-12-22

    Applicant: BASF AG

    Inventor： HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA

    IPC: C12N15/11 ,  C12N1/21

    Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

    80.
    发明专利
    未知

    公开(公告)号：BR0317507A

    公开(公告)日：2005-11-16

    申请号：BR0317507

    申请日：2003-12-19

    Applicant: BASF AG

    Inventor： KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HOFNER STEFAN ,  LIEBL WOLFGANG

    IPC: C12P13/04

    Abstract: The invention relates to a method for producing an amino acid comprising culturing a microorganism of the genus Corynebacterium or Brevibacterium wherein said microorganism is partially or completely deficient in at least one of the gene loci of the the group which is formed by otsAB, treZ and treS, and subsequent isolation of the amino acid from the culture medium.

Patent Agency Ranking