동물의 면역능을 증강시키는 더덕 추출물, 그 제조방법 및용도
    3.
    发明公开
    동물의 면역능을 증강시키는 더덕 추출물, 그 제조방법 및용도 有权
    提取动物免疫功能强度的方法和用途

    公开(公告)号:KR1020090028213A

    公开(公告)日:2009-03-18

    申请号:KR1020070093644

    申请日:2007-09-14

    Abstract: A manufacturing method of extract of codonopsis lanceolata is provided to increase immunomodulation enhancement of pathogenic bacteria, to be useful as immunostimulator preventing and treating efficiently disease of the animal, an adjuvant improving effect of vaccine and an adjuvant treatment making therapeutic efficacy reinforced. A manufacturing method of extract of codonopsis lanceolata comprises steps of: obtaining the first extracts and residue by heating powder of leaf and stem of the dried Condonopsis lanceolata Trautv into distilled water, separating the first extracts separately, putting it into residue, heating it obtaining the second extraction extracts; mixing the first extracts of the obtained Condonopsis lanceolata Trautv stem and leaf and the second extracts of Condonopsis lanceolata Trautv stem and leaf, putting ethanol into the mixing extract, obtaining precipitated polysaccharide by stationing the obtained solution and centrifuging the precipitated polysaccharide; and center-rinsing the separated polysaccharide to the ethanol, drying the washed material, dissolving dried material with distilled water, filtering the obtained solution and obtaining undiluted solution.

    Abstract translation: 提供密码子提取物的制造方法,以增加病原菌的免疫调节增强作用,有效地用于预防和治疗动物有效疾病的免疫刺激剂,疫苗的辅助改善效果和辅助治疗,增强治疗功效。 密码子提取物的制造方法包括以下步骤:通过将干燥的马铃薯干燥杆菌的叶和茎的粉末加热到蒸馏水中获得第一提取物和残留物,分离第一提取物,将其放入残渣中,加热得到 第二提取提取物; 混合所得到的Condonopsis lanceolata Trautv茎和叶的第一提取物和Condonopsis lanceolata Trautv茎和叶的第二提取物,将乙醇加入到混合提取物中,通过沉淀所得溶液并离心沉淀的多糖得到沉淀的多糖; 将分离的多糖中心漂洗至乙醇,干燥洗涤过的物质,用蒸馏水溶解干燥物,过滤得到的溶液,得到未稀释溶液。

    동물의 면역능을 증강시키는 지네 추출물, 그 제조방법 및용도
    4.
    发明公开
    동물의 면역능을 증강시키는 지네 추출물, 그 제조방법 및용도 无效
    提取动物强力强度的准确性及其使用方法

    公开(公告)号:KR1020090028212A

    公开(公告)日:2009-03-18

    申请号:KR1020070093643

    申请日:2007-09-14

    CPC classification number: A61K35/648

    Abstract: A manufacturing method of Scolopendra subspinipes extract making immunogenicity of animal is provided to be useful as immunostimulator preventing and treating efficiently disease of the animal, an adjuvant improving effect of vaccine and an adjuvant treatment making therapeutic efficacy reinforced. A manufacturing method of Scolopendra subspinipes extract making immunogenicity of animal comprises steps of: put the Scolopendra subspinipes powder into distilled water; obtaining extract by heating obtained solution, filtering obtained extract using filter paper firstly, filtering filtered material using filter paper secondly and sterilizing filtered material.

    Abstract translation: 提供了提供动物免疫原性的Scolopendra subspinipes提取物的制造方法,可用作有效预防和治疗动物疾病的免疫刺激剂,疫苗的辅助改善效果和辅助治疗,增强治疗功效。 Scolopendra subspinipes提取物制备动物免疫原性的方法包括以下步骤:将Scolopendra subspinipes粉末放入蒸馏水中; 通过加热获得的溶液获得提取物,首先使用滤纸过滤获得的提取物,然后用滤纸过滤过滤材料并对过滤材料进行灭菌。

    캔디다 사케 용균 추출물을 포함하는 면역 증강제
    5.
    发明公开
    캔디다 사케 용균 추출물을 포함하는 면역 증강제 无效
    免疫球蛋白包括坎地迪沙星的提取物

    公开(公告)号:KR1020090013600A

    公开(公告)日:2009-02-05

    申请号:KR1020070077855

    申请日:2007-08-02

    CPC classification number: A61K36/06

    Abstract: An immunostimulant containing the lystic extract of Candida sake is provided to increase activity of neutrophil and lymphocyte as major immune cells of animals, thereby protecting animals from diseases. The immunostimulant contains the lystic extract of Candida sake which is prepared by cultivating Candida sake in yeast malt extract agar broth at 30 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Candida sake suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.

    Abstract translation: 提供含有念珠菌精液提取物的免疫刺激剂,以增加嗜中性粒细胞和淋巴细胞作为动物主要免疫细胞的活性,从而保护动物免受疾病。 免疫刺激剂含有通过在30℃下在酵母麦芽提取物琼脂培养液中培育念珠菌精油而制备的念珠菌清酒。 培养72小时,将培养液以3000Xg离心30分钟并收集,用pH7.2的磷酸盐缓冲盐水(PBS)冲洗收集的沉淀物,并将漂洗的沉淀悬浮于PBS中,加入5%含有50mM Tris -HCl,0.15M NaCl和4%Triton x-114作为增溶剂加入到念珠菌悬浮液中,在室温下搅拌混合物2-3天以溶解链霉菌,并将溶液的pH调节至6.5-7.2 ,并干燥恒温室中的溶液。

    동물의 면역능을 증강시키는 더덕 추출물, 그 제조방법 및용도
    10.
    发明授权
    동물의 면역능을 증강시키는 더덕 추출물, 그 제조방법 및용도 有权
    提取动物免疫强度的党参提取物,方法与用途

    公开(公告)号:KR100951055B1

    公开(公告)日:2010-04-06

    申请号:KR1020070093644

    申请日:2007-09-14

    Abstract: 본 발명은 동물의 면역능을 증강시키는 더덕 추출물 및 그 제조방법과 용도에 관한 것이다. 보다 상세하게는 더덕줄기 및 잎을 음지에서 건조시켜 마쇄하여 열탕용 봉지에 넣고 증류수 중에 가열하여 1차 추출한 후, 추출물은 별도로 분리하고 잔류물에 재차 증류수를 넣고 열탕하여 2차 추출하여 추출물을 얻는 단계와, 1차 및 2차 추출물을 혼합하여 추출물에 2배량의 에탄올을 가하여 4∼6℃ 냉장실에서 24시간 정치시켜 침전된 다당체를 원심분리하는 단계와, 분리된 다당체를 순수 에탄올로 원심 세척한 후 35∼38℃ 항온실에서 건조하여 증류수로 1mg/ml 농도로 용해시켜 세균여과지로 여과하여 원액을 얻는 단계로 구성된다.
    본 발명의 더덕추출물은 동물의 주요 면역세포인 호중구의 각종 활성과 싸이토카인 생성을 증강시키고 병원성세균의 공격접종에 대한 비특이적인 방어능을 증진시키는 효과가 있으므로 동물용 의약품으로써 면역증강제. 백신보좌제 첨가물 그리고 보조치료제 등으로 광범위하게 활용될 수 있을 것이다.

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