公开(公告)号：KR1020110052295A

公开(公告)日：2011-05-18

申请号：KR1020090109275

申请日：2009-11-12

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 조윤상 ,  오진식 ,  조동희 ,  황인영 ,  허문 ,  정석찬 ,  양창범 ,  장영부 ,  강성일 ,  하건우 ,  오연경 ,  정승교

IPC: G01N33/68 ,  C07K14/35 ,  C12R1/32

Abstract: PURPOSE: A method for diagnosing cattle tuberculosis using intradermal diagnostic composition is provided to easily prepare a large amount of intradermal diagnostic composition and to ensure diagnosis accuracy. CONSTITUTION: An intradermal diagnostic composition for testing cattle tuberculosis contains recombinant proteins of Esat6, HspX, PhoS, MPB70, MPB64, MPB83, Ag85, and SahH. A method for diagnosing tuberculosis of mammal except for human is performed using the intradermal diagnostic composition containing the recombinant proteins. The recombinant protein is prepared by isolating from Mycobacterium bovis and performing PCR.

Abstract translation: 目的：提供使用皮内诊断组合物诊断牛结核病的方法，以容易地制备大量皮内诊断组合物并确保诊断准确性。 构成：用于测试牛结核的皮内诊断组合物含有Esat6，HspX，PhoS，MPB70，MPB64，MPB83，Ag85和SahH的重组蛋白。 使用含有重组蛋白质的皮内诊断组合物进行用于诊断除人以外的哺乳动物结核病的方法。 通过从牛分枝杆菌中分离并进行PCR来制备重组蛋白。

公开(公告)号：KR1020110044343A

公开(公告)日：2011-04-29

申请号：KR1020090100960

申请日：2009-10-23

Applicant: 서울대학교산학협력단 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이문한 ,  강가영 ,  강빛나 ,  조영식 ,  오진식 ,  하건우 ,  오연경 ,  정승교 ,  한창훈 ,  나병승 ,  문서정

IPC: G01N33/12 ,  G01N33/573

Abstract: PURPOSE: A method for distinguishing frozen-thawed meat and refrigerated meat is provided to ensure simple and easy use without expensive equipment. CONSTITUTION: A composition for distinguishing frozen-thawed meat and refrigerated meat contains an antibody with respect to aspartate aminotransferase(AST) allozyme. AST allozyme is mitochondria AST. The meat is beef, pork, or chicken. A method for distinguishing frozen-thawed meat and refrigerated meat comprises: a step of preparing meat juice as a biological sample; and a step of performing electrophoresis of the meat juice to detect AST allozyme.

Abstract translation: 目的：提供一种区分冷冻解冻肉和冷藏肉的方法，以确保简单易用，无需昂贵的设备。 构成：用于区分冷冻解冻的肉和冷冻肉的组合物含有针对天冬氨酸氨基转移酶（AST）同种异体酶的抗体。 AST等位酶是线粒体AST。 肉是牛肉，猪肉或鸡肉。 一种区分冷冻解冻肉和冷冻肉的方法包括：制备肉汁作为生物样品的步骤; 以及进行肉汁的电泳以检测AST同种异体酶的步骤。