公开(公告)号：KR1020120051196A

公开(公告)日：2012-05-22

申请号：KR1020100112506

申请日：2010-11-12

Applicant: 주식회사 바이오노트 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 조영식 ,  하건우 ,  오진식 ,  강동석 ,  오연경 ,  박정애 ,  한선호 ,  구복경 ,  정석찬 ,  허문 ,  정갑수 ,  황인영 ,  조윤상

IPC: C07K16/28 ,  G01N33/53

Abstract: PURPOSE: A kit for diagnosing bovine tuberculosis containing monoclonal antibodies and polyclonal antibodies is provided to effectively measure tuberculosis attack. CONSTITUTION: A monoclonal antibody which specifically binds to an epitope is denoted by an amino acid sequence of sequence number 8 in a bovine interferon gamma receptor-binding region. The monoclonal antibody is produced from hydridoma cells(deposit number KCTC 11784BP). A kit for diagnosing bovine contains monoclonal antibody or antigen binding fragment and anti-bovine interferon gamma polyclonal antibody.

Abstract translation: 目的：提供用于诊断含有单克隆抗体和多克隆抗体的牛结核的试剂盒，以有效测量结核病的发作。 构成：特异性结合表位的单克隆抗体由牛干扰素γ受体结合区中的序列号8的氨基酸序列表示。 单克隆抗体由Hydridoma细胞（保藏号KCTC 11784BP）产生。 用于诊断牛的试剂盒含有单克隆抗体或抗原结合片段和抗牛干扰素γ多克隆抗体。

公开(公告)号：KR101263913B1

公开(公告)日：2013-05-14

申请号：KR1020100112506

申请日：2010-11-12

Applicant: 주식회사 바이오노트 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 조영식 ,  하건우 ,  오진식 ,  강동석 ,  오연경 ,  박정애 ,  한선호 ,  구복경 ,  정석찬 ,  허문 ,  정갑수 ,  황인영 ,  조윤상

IPC: C07K16/28 ,  G01N33/53

Abstract: 소의인터페론감마에특이적으로결합하는단일클론항체및 폴리클론항체를포함하는우결핵진단키트및 이를이용한우결핵진단방법에관한것이다. 일구체예에따른소의인터페론감마에특이적으로결합하는단일클론항체, 폴리클론항체를포함하는우결핵진단키트및 이를이용한우결핵진단방법에따르면, 소과동물의우결핵발병여부를효율적으로측정할수 있다.

公开(公告)号：KR1020110052295A

公开(公告)日：2011-05-18

申请号：KR1020090109275

申请日：2009-11-12

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 조윤상 ,  오진식 ,  조동희 ,  황인영 ,  허문 ,  정석찬 ,  양창범 ,  장영부 ,  강성일 ,  하건우 ,  오연경 ,  정승교

IPC: G01N33/68 ,  C07K14/35 ,  C12R1/32

Abstract: PURPOSE: A method for diagnosing cattle tuberculosis using intradermal diagnostic composition is provided to easily prepare a large amount of intradermal diagnostic composition and to ensure diagnosis accuracy. CONSTITUTION: An intradermal diagnostic composition for testing cattle tuberculosis contains recombinant proteins of Esat6, HspX, PhoS, MPB70, MPB64, MPB83, Ag85, and SahH. A method for diagnosing tuberculosis of mammal except for human is performed using the intradermal diagnostic composition containing the recombinant proteins. The recombinant protein is prepared by isolating from Mycobacterium bovis and performing PCR.

Abstract translation: 目的：提供使用皮内诊断组合物诊断牛结核病的方法，以容易地制备大量皮内诊断组合物并确保诊断准确性。 构成：用于测试牛结核的皮内诊断组合物含有Esat6，HspX，PhoS，MPB70，MPB64，MPB83，Ag85和SahH的重组蛋白。 使用含有重组蛋白质的皮内诊断组合物进行用于诊断除人以外的哺乳动物结核病的方法。 通过从牛分枝杆菌中分离并进行PCR来制备重组蛋白。

公开(公告)号：KR1020110044343A

公开(公告)日：2011-04-29

申请号：KR1020090100960

申请日：2009-10-23

Applicant: 서울대학교산학협력단 ,  대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이문한 ,  강가영 ,  강빛나 ,  조영식 ,  오진식 ,  하건우 ,  오연경 ,  정승교 ,  한창훈 ,  나병승 ,  문서정

IPC: G01N33/12 ,  G01N33/573

Abstract: PURPOSE: A method for distinguishing frozen-thawed meat and refrigerated meat is provided to ensure simple and easy use without expensive equipment. CONSTITUTION: A composition for distinguishing frozen-thawed meat and refrigerated meat contains an antibody with respect to aspartate aminotransferase(AST) allozyme. AST allozyme is mitochondria AST. The meat is beef, pork, or chicken. A method for distinguishing frozen-thawed meat and refrigerated meat comprises: a step of preparing meat juice as a biological sample; and a step of performing electrophoresis of the meat juice to detect AST allozyme.

Abstract translation: 目的：提供一种区分冷冻解冻肉和冷藏肉的方法，以确保简单易用，无需昂贵的设备。 构成：用于区分冷冻解冻的肉和冷冻肉的组合物含有针对天冬氨酸氨基转移酶（AST）同种异体酶的抗体。 AST等位酶是线粒体AST。 肉是牛肉，猪肉或鸡肉。 一种区分冷冻解冻肉和冷冻肉的方法包括：制备肉汁作为生物样品的步骤; 以及进行肉汁的电泳以检测AST同种异体酶的步骤。