Abstract:
PURPOSE: An H9 type avian influenza virus diagnosis kit by quick immunochromatography is provided to ensure quick and simple diagnosis without special equipment. CONSTITUTION: A kit for diagnosing H9 type avian influenza virus contains monoclonal antibodies. The monoclonal antibody is prepared from hybridoma cells of deposit number 11753BP, KCTC 11754BP, and KCTC 11755BP. A H9 type avian influenza diagnosis strip contains the monoclonal antibody. The diagnosis strip comprises a sample pad(1), a gold pad(2), nitrocellulose membrane(3), and moisture absorption pad(4). The gold pad contains a gold conjugate in which gold particles are conjugated with a monoclonal antibody produced from hybridoma cells of deposit number KCTC 11755BP.
Abstract:
PURPOSE: A kit for diagnosing bovine tuberculosis containing monoclonal antibodies and polyclonal antibodies is provided to effectively measure tuberculosis attack. CONSTITUTION: A monoclonal antibody which specifically binds to an epitope is denoted by an amino acid sequence of sequence number 8 in a bovine interferon gamma receptor-binding region. The monoclonal antibody is produced from hydridoma cells(deposit number KCTC 11784BP). A kit for diagnosing bovine contains monoclonal antibody or antigen binding fragment and anti-bovine interferon gamma polyclonal antibody.
Abstract:
PURPOSE: A method for diagnosing cattle tuberculosis using intradermal diagnostic composition is provided to easily prepare a large amount of intradermal diagnostic composition and to ensure diagnosis accuracy. CONSTITUTION: An intradermal diagnostic composition for testing cattle tuberculosis contains recombinant proteins of Esat6, HspX, PhoS, MPB70, MPB64, MPB83, Ag85, and SahH. A method for diagnosing tuberculosis of mammal except for human is performed using the intradermal diagnostic composition containing the recombinant proteins. The recombinant protein is prepared by isolating from Mycobacterium bovis and performing PCR.
Abstract:
PURPOSE: A method for distinguishing frozen-thawed meat and refrigerated meat is provided to ensure simple and easy use without expensive equipment. CONSTITUTION: A composition for distinguishing frozen-thawed meat and refrigerated meat contains an antibody with respect to aspartate aminotransferase(AST) allozyme. AST allozyme is mitochondria AST. The meat is beef, pork, or chicken. A method for distinguishing frozen-thawed meat and refrigerated meat comprises: a step of preparing meat juice as a biological sample; and a step of performing electrophoresis of the meat juice to detect AST allozyme.
Abstract:
본 발명은 조류 인플루엔자 바이러스 항체의 진단키트 및 이를 이용한 진단방법에 관한 것이다. 보다 구체적으로 본 발명은 조류 인플루엔자 바이러스의 핵단백질(Nucleoprotein)에 대한 단클론 항체가 흡착된 마이크로플레이트에 재조합된 핵단백질 항원이 결합된 인플루엔자 항원 흡착 플레이트 및 조류 인플루엔자 바이러스의 핵단백질에 대한 단클론 항체와 효소가 접합된 효소표지항체를 포함하는 것을 특징으로 하는 조류 인플루엔자 바이러스 항체의 진단키트 및 이를 이용하여 검사시료 내 조류 인플루엔자 바이러스 항체를 측정하는 것을 특징으로 하는 진단방법에 관한 것이다. 조류 인플루엔자, 핵단백질, 단클로 항체, 효소표지항체
Abstract:
본 발명은 조류 인플루엔자 바이러스 항체의 진단키트 및 이를 이용한 진단방법에 관한 것이다. 보다 구체적으로 본 발명은 조류 인플루엔자 바이러스의 핵단백질(Nucleoprotein)에 대한 단클론 항체가 흡착된 마이크로플레이트에 재조합된 핵단백질 항원이 결합된 인플루엔자 항원 흡착 플레이트 및 조류 인플루엔자 바이러스의 핵단백질에 대한 단클론 항체와 효소가 접합된 효소표지항체를 포함하는 것을 특징으로 하는 조류 인플루엔자 바이러스 항체의 진단키트 및 이를 이용하여 검사시료 내 조류 인플루엔자 바이러스 항체를 측정하는 것을 특징으로 하는 진단방법에 관한 것이다. 조류 인플루엔자, 핵단백질, 단클로 항체, 효소표지항체
Abstract:
A kit and a method for diagnosing a porcine epidemic diarrhea virus antigen are provided to diagnose the antigen conveniently and rapidly without a special testing equipment in a farm or a laboratory field. A kit for diagnosing a porcine epidemic diarrhea virus antigen is characterized in that a conjugate of a monoclonal antibody 6G15(deposition no. KCTC 10996BP) coupled to nucleocapside 58kDa of an epidemic diarrhea virus and a gold particle is attached to a reaction position of the porcine epidemic diarrhea virus antigen on a strip and a monoclonal antibody 9D11(deposition no. KCTC 10997BP) is attached to a position different from the coupling portion of the porcine epidemic diarrhea virus antigen and the monoclonal antibody 6G15. A method for diagnosing the porcine epidemic diarrhea virus antigen comprises the steps of: (a) loading a sample onto a detection pad on a strip; (b) reacting the conjugate of the monoclonal antibody 6G15 coupled to nucleocapside 58kDa of the epidemic diarrhea virus and the gold particle with the porcine epidemic diarrhea virus antigen in the sample; and (c) reacting the porcine epidemic diarrhea virus antigen, the monoclonal antibody 9D11 and the reaction product obtained from the step(b).