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公开(公告)号:KR1020130090609A
公开(公告)日:2013-08-14
申请号:KR1020120011866
申请日:2012-02-06
Applicant: 주식회사농심
Abstract: PURPOSE: A high throughput system for screening salt enhancing materials is provided to reduce the content of NaCl of foods without affecting salty taste and to resolve technical problems of a study using gustatory cells. CONSTITUTION: A high throughput system for screening salt enhancing materials selects salt enhancing materials which change a tight junction structure of gustatory cells using a human cell line. The human cell line generates claudin proteins and is selected among: prostate cancer cell lines such as LNCaP, ALVA41, DU145, and PC3; ovarian cancer cell lines such as PE04, OVCA429, OVCAR3, OVCA433, CAOV3, and MCAS; colon cancer cell lines such as T84; kidney cancer cell lines such as MDCK-II; and pancreatic cancer cell lines such as PANC-1. [Reference numerals] (AA) Intensity of fluorescence (340/380); (BB) Functionality score
Abstract translation: 目的:提供筛选盐增强材料的高通量系统,以减少食物中NaCl的含量,而不影响咸味,并解决使用味觉细胞的研究的技术问题。 构成:用于筛选盐增强材料的高通量系统选择盐增强材料,其使用人细胞系改变味觉细胞的紧密结合结构。 人细胞系产生克鲁丁蛋白,选自:前列腺癌细胞系如LNCaP,ALVA41,DU145和PC3; 卵巢癌细胞系如PE04,OVCA429,OVCAR3,OVCA433,CAOV3和MCAS; 结肠癌细胞系如T84; 肾癌细胞系如MDCK-II; 和胰腺癌细胞系如PANC-1。 (AA)荧光强度(340/380); (BB)功能得分
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公开(公告)号:KR101316741B1
公开(公告)日:2013-10-08
申请号:KR1020120011866
申请日:2012-02-06
Applicant: 주식회사농심
Abstract: 본 발명의 일 측면은 클라우딘류 단백질을 생성하는 인간 세포 라인을 이용하여 미각 세포의 밀착연접(tight junction)의 구조를 변화시키는 염미 강화물질을 선별하는 염미 강화물질 선별 시스템을 제공한다.
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