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公开(公告)号:KR101316741B1
公开(公告)日:2013-10-08
申请号:KR1020120011866
申请日:2012-02-06
Applicant: 주식회사농심
Abstract: 본 발명의 일 측면은 클라우딘류 단백질을 생성하는 인간 세포 라인을 이용하여 미각 세포의 밀착연접(tight junction)의 구조를 변화시키는 염미 강화물질을 선별하는 염미 강화물질 선별 시스템을 제공한다.
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公开(公告)号:KR101239624B1
公开(公告)日:2013-03-07
申请号:KR1020100138569
申请日:2010-12-30
Applicant: 주식회사농심
Abstract: 본 발명은 고 프로테아제 및 글루타미나아제 활성을 가진 균주를 선정하여 식물성 단백질을 잡균의 오염 없이 아스퍼질러스 오리제(
Aspergillus
oryzae )의 액상 배양액과 혼합하여 가수분해 공정을 실시함으로써 감칠맛이 우수한 식물성 단백질의 가수분해물을 제조하고자 한다.-
公开(公告)号:KR1020130090609A
公开(公告)日:2013-08-14
申请号:KR1020120011866
申请日:2012-02-06
Applicant: 주식회사농심
Abstract: PURPOSE: A high throughput system for screening salt enhancing materials is provided to reduce the content of NaCl of foods without affecting salty taste and to resolve technical problems of a study using gustatory cells. CONSTITUTION: A high throughput system for screening salt enhancing materials selects salt enhancing materials which change a tight junction structure of gustatory cells using a human cell line. The human cell line generates claudin proteins and is selected among: prostate cancer cell lines such as LNCaP, ALVA41, DU145, and PC3; ovarian cancer cell lines such as PE04, OVCA429, OVCAR3, OVCA433, CAOV3, and MCAS; colon cancer cell lines such as T84; kidney cancer cell lines such as MDCK-II; and pancreatic cancer cell lines such as PANC-1. [Reference numerals] (AA) Intensity of fluorescence (340/380); (BB) Functionality score
Abstract translation: 目的:提供筛选盐增强材料的高通量系统,以减少食物中NaCl的含量,而不影响咸味,并解决使用味觉细胞的研究的技术问题。 构成:用于筛选盐增强材料的高通量系统选择盐增强材料,其使用人细胞系改变味觉细胞的紧密结合结构。 人细胞系产生克鲁丁蛋白,选自:前列腺癌细胞系如LNCaP,ALVA41,DU145和PC3; 卵巢癌细胞系如PE04,OVCA429,OVCAR3,OVCA433,CAOV3和MCAS; 结肠癌细胞系如T84; 肾癌细胞系如MDCK-II; 和胰腺癌细胞系如PANC-1。 (AA)荧光强度(340/380); (BB)功能得分
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公开(公告)号:KR1020120076841A
公开(公告)日:2012-07-10
申请号:KR1020100138569
申请日:2010-12-30
Applicant: 주식회사농심
Abstract: PURPOSE: A producing method of vegetable protein hydrolysate having excellent savory taste is provided to mix vegetable protein with culture fluid of aspergillus oryzae without the contamination by other microorganisms. CONSTITUTION: A producing method of vegetable protein hydrolysate comprises the following steps: grinding aspergillus oryzae into 0.5-3mm; culturing aspergillus in culture fluid; preparing a dispersed solution of vegetable protein; injecting the aspergillus oryzae into the dispersed solution of the vegetable protein for fermenting the vegetable protein; and refining the fermented vegetable protein, and pulverizing to obtain powder. The culture fluid contains 5-20g/L of organic nitrogen source, 5-20g/L of sterilized glucose, 5-20g/L of enzyme extract, 1-10g/L of potassium phosphate, and 0.1-2g/L of magnesium sulfate. The vegetable protein is soybean protein, bean protein, corn protein, or rice protein having the average particle size of 10-100 micrometers.
Abstract translation: 目的:提供具有优良口味的植物蛋白水解产物的生产方法,将植物蛋白与米曲霉培养液混合而不受其他微生物的污染。 构成:植物蛋白水解产物的制备方法包括以下步骤:将米曲霉加工成0.5-3mm; 在培养液中培养曲霉菌; 制备植物蛋白的分散溶液; 将米曲霉注射到用于发酵植物蛋白质的植物蛋白质的分散溶液中; 精制发酵的植物蛋白,粉碎,得到粉末。 培养液含有5-20g / L有机氮源,5-20g / L灭菌葡萄糖,5-20g / L酶提取物,1-10g / L磷酸钾和0.1-2g / L硫酸镁 。 植物蛋白是平均粒径为10-100微米的大豆蛋白,豆蛋白,玉米蛋白或大米蛋白。
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