MACROPOROUS SUPPORT FOR CHEMICAL AMPLIFICATION REACTION AND ITS UTILIZATION

    公开(公告)号:JP2006223309A

    公开(公告)日:2006-08-31

    申请号:JP2006043178

    申请日:2006-02-20

    Abstract: PROBLEM TO BE SOLVED: To provide a method for amplifying a nucleic acid in high accuracy and in high repeatability, and to provide an apparatus therefor. SOLUTION: This method for amplifying the nucleic acid in the holes 2 of a macroporous support comprises (a) a process for supplying the prescribed components of a reaction mixture suitable for the amplification reaction to the first region having at least two holes 2 of the support and binding the components of the reaction mixture to the hole walls 1 of the support through non-covalent bonds to form a reaction region on the surfaces of the support, wherein the many separated holes 2 having diameters of 500 nm to 100 μm and a depth : opening aspect ratio of at least 10:1 are dispersed in the first surface and the second surface in a density of 10 4 to 10 8 /cm 2 , (b) a process for adding a sample containing the components of the reaction mixture required for the performance of the amplification reaction to the whole support, (c) a process for performing the amplification reaction, and (d) a process for detecting at least one amplification product. COPYRIGHT: (C)2006,JPO&NCIPI

    5.
    发明专利
    未知

    公开(公告)号:DE102007019552A1

    公开(公告)日:2008-10-30

    申请号:DE102007019552

    申请日:2007-04-25

    Abstract: A substrate with first and second main surfaces includes at least one channel extending from the first main surface to the second main surface. The at least one channel includes a first cross-sectional area at a first location and a second cross-sectional area at a second location. An electrically conductive first material is disposed in the at least one channel.

    8.
    发明专利
    未知

    公开(公告)号:DE102007019552B4

    公开(公告)日:2009-12-17

    申请号:DE102007019552

    申请日:2007-04-25

    Abstract: A substrate with first and second main surfaces includes at least one channel extending from the first main surface to the second main surface. The at least one channel includes a first cross-sectional area at a first location and a second cross-sectional area at a second location. An electrically conductive first material is disposed in the at least one channel.

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