公开(公告)号：US09305757B2

公开(公告)日：2016-04-05

申请号：US14369344

申请日：2012-12-06

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/42 ,  H01J49/26 ,  H01J49/06

CPC classification number: H01J49/06 ,  H01J49/26 ,  H01J49/4225 ,  H01J49/4255

Abstract: A method is provided for processing ions in a multipole ion trap, comprising generating RF radial confinement fields within a first and second multipole rod set positioned in tandem, a ratio of q value exhibited by the second rod set relative to the first rod set being greater than one for any m/z, said RF axial confinement fields within the first and second rod sets interacting in an interaction region between the first and second rod sets so as to produce a fringing field; transmitting ions through said first rod set towards said second rod set; and increasing the radial oscillation amplitude of at least a portion of the ions within said first rod set such that at least a portion of said ions having an increased radial oscillation amplitude are repulsed by said fringing field.

Abstract translation: 提供了一种用于处理多极离子阱中的离子的方法，包括在串联定位的第一和第二多极棒组内产生RF径向限制场，第二棒组相对于第一棒组显示的q值的比值更大 所述第一和第二杆组内的所述RF轴向限制场在所述第一和第二杆组之间的相互作用区域中相互作用以产生边缘场; 将离子通过所述第一杆组传递到所述第二杆组; 并且增加所述第一棒组中的离子的至少一部分的径向振荡振幅，使得具有增加的径向振荡幅度的所述离子的至少一部分被所述边缘场排斥。

公开(公告)号：US20160079048A1

公开(公告)日：2016-03-17

申请号：US14944467

申请日：2015-11-18

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Ronald F. Bonner ,  Stephen A. Tate

IPC: H01J49/00

CPC classification number: H01J49/0045 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/04

Abstract: Systems and methods are used to rapidly screening samples. A fast sample introduction device that is non-chromatographic is instructed to supply each sample of a plurality samples to a tandem mass spectrometer using a processor. The fast sample introduction device can include a flow injection analysis device, an ion mobility analysis device, or a rapid sample cleanup device. The tandem mass spectrometer is instructed to perform fragmentation scans at two or more mass selection windows across a mass range of each sample of the plurality of samples using the processor. The two or more mass selection windows across the mass range can have fixed or variable window widths. The tandem mass spectrometer can be instructed to obtain a mass spectrum of the mass range before instructing the tandem mass spectrometer to perform the fragmentation scans.

公开(公告)号：US09202677B2

公开(公告)日：2015-12-01

申请号：US14401032

申请日：2013-04-19

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Stephen A. Tate ,  Ronald F. Bonner

IPC: H01J49/40 ,  B01D59/44 ,  H01J49/00

CPC classification number: H01J49/0045 ,  G01N30/7233 ,  G01N30/8631 ,  G06F19/703 ,  H01J49/0009 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004

Abstract: Systems and methods are provided for analyzing a sample using overlapping measured mass selection window widths. A mass range of a sample is divided into two or more target mass selection window widths using a processor. The two or more target widths can have the same width or variable widths. A tandem mass spectrometer is instructed to perform two or more fragmentation scans across the mass range using the processor. Each fragmentation scan of the two or more fragmentation scans includes a measured mass selection window width. The two or more measured widths of the two or more fragmentation scans can have the same width or variable widths. At least two of the two or more measured mass selection window widths overlap. The overlap in measured mass selection window widths corresponds to at least one target mass selection window width.

Abstract translation: 提供了系统和方法，用于使用重叠的测量质量选择窗口宽度来分析样本。 使用处理器将样本的质量范围分为两个或更多个目标质量选择窗口宽度。 两个或多个目标宽度可以具有相同的宽度或可变宽度。 指示串联质谱仪使用处理器在质量范围内执行两次或更多次碎片扫描。 两个或多个碎片扫描的每个碎片扫描包括测量的质量选择窗口宽度。 两个或更多个碎片扫描的两个或多个测量宽度可以具有相同的宽度或可变宽度。 两个或更多个测量质量选择窗口宽度中的至少两个重叠。 测量的质量选择窗口宽度中的重叠对应于至少一个目标质量选择窗口宽度。

公开(公告)号：US20150332904A1

公开(公告)日：2015-11-19

申请号：US14443933

申请日：2013-11-21

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell

IPC: H01J49/00

CPC classification number: H01J49/005 ,  H01J49/0031

Abstract: Systems and methods are provided for reducing the time period of a CID event of an MS3 experiment and making the overall fragmentation event more generic. A CID event of an MS3 experiment performed on a sample by a mass spectrometer is divided into two time periods using a processor. At the beginning of a first time period of the CID event, the mass spectrometer is instructed to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage. At the beginning of a second time period of the CID event, the mass spectrometer is instructed to both close the pulse valve and apply a second CID voltage. The mass spectrometer is pumped down during the second time period. The overlap in time of the pump down and CID reduces the overall time period of the CID event.

Abstract translation: 提供了系统和方法来减少MS3实验的CID事件的时间周期，并使整个碎片事件更为通用。 通过质谱仪对样品进行的MS3实验的CID事件使用处理器分成两个时间段。 在CID事件的第一时间段开始时，指示质谱仪打开脉冲阀以便脉冲碰撞气体并施加第一CID电压。 在CID事件的第二时间段开始时，指示质谱仪关闭脉冲阀并施加第二CID电压。 质谱仪在第二时间内被抽出。 泵送时间和CID的重叠减少了CID事件的总体时间。

公开(公告)号：US09188596B2

公开(公告)日：2015-11-17

申请号：US14104117

申请日：2013-12-12

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Subhakar Dey ,  Sasi Pillal ,  Brian L. Williamson ,  Subhasish Purkayastha

IPC: G01N33/82 ,  G01N30/72

CPC classification number: G01N33/82 ,  Y10T436/20 ,  Y10T436/200833 ,  Y10T436/203332 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

Abstract: Quantification of vitamin D2, vitamin D3, and the monohydroxy and diihydroxy metabolites of vitamin D2 and vitamin D3, can comprise labeling analytes with mass spectrometry (MS) tagging reagents and performing LC-MSMS analysis of the labeled analytes. The labeled analytes can include a labeled standard and can have distinct retention times on a reversed phase column, as well as distinct masses. Under high energy collisions, reporter groups can be generated. The intensity or the peak area detected for each reporter group can be used for quantitation. In some embodiments, a one-step tagging reagent is used that is a dienophile-containing, labeled m Diels Alder reagent.

Abstract translation: 维生素D 2，维生素D3和维生素D 2和维生素D3的单羟基和二羟基代谢物的定量可以包括用质谱法（MS）标记试剂标记分析物并对标记的分析物进行LC-MSMS分析。 标记的分析物可以包括标记的标准物，并且可以在反相柱上具有不同的保留时间，以及不同的质量。 在高能量碰撞下，可以产生记者组。 每个报道组检测到的强度或峰面积可用于定量。 在一些实施方案中，使用一种含有亲二烯体的标记的狄尔斯·阿德尔试剂的一步标签试剂。

公开(公告)号：US20150316507A1

公开(公告)日：2015-11-05

申请号：US14655263

申请日：2013-12-13

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John L. CAMPBELL ,  Yves LEBLANC

IPC: G01N27/62 ,  H01J49/04 ,  H01J49/00

CPC classification number: G01N27/624 ,  G01N27/622 ,  G01N33/92 ,  G01N2458/15 ,  H01J49/0031 ,  H01J49/0422 ,  Y10T436/24

Abstract: A method for analyzing a sample that contains a plurality of lipid isomers is described that involves forming one or more lipid metal ion adducts and transporting the one or more lipid metal ion adducts through a Mix of POPC+OPPC differential mobility spectrometer to cause separation of the one or more lipid metal ion adducts from each other. The lipid isomers can be chosen, for example, from fatty acids, glycerolipids, glycerophospholipids, sphingolipids, saccharolipids, polyketides, sterol lipids, and prenol lipids. Particular examples include phosphatidylcholine regioisomers such as 1-palmatoyl-2-oleoyl-sn-phosphatidylcholine (POPC) and 1-oleoyl-2-palmatoyl-sn-phosphatidylcholine and triacylglycerols containg palmetic and oleic acid groups. The metal chosen can include a cationization reagent that contains sodium, potassium, silver or lithium.

Abstract translation: 描述了分析含有多种脂质异构体的样品的方法，其涉及形成一种或多种脂质金属离子加合物，并通过POPC + OPPC差速迁移谱仪的混合物输送一种或多种脂质金属离子加合物，以使 一种或多种彼此之间的脂质金属离子加成物。 脂质异构体可以选自例如脂肪酸，甘油脂，甘油磷脂，鞘脂，蔗糖脂，聚酮化合物，甾醇脂质和牛油醇脂质。 具体实例包括磷脂酰胆碱区域异构体，例如1-棕榈酰-2-油酰-sn-磷脂酰胆碱（POPC）和1-油酰-2-棕榈酰-sn-磷脂酰胆碱和含有油酸和油酸基团的三酰基甘油。 所选择的金属可以包括含有钠，钾，银或锂的阳离子化试剂。

公开(公告)号：US09134273B2

公开(公告)日：2015-09-15

申请号：US14397236

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Yves LeBlanc

IPC: G01N33/50 ,  B01D59/44 ,  G01N27/62 ,  H01J49/00

CPC classification number: G01N27/622 ,  G01N27/624 ,  G01N33/50 ,  G01N2800/02 ,  H01J49/00

Abstract: Methods and systems for performing ion mobility spectrometry are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of biologically relevant acids that may be difficult to separate with conventional MS techniques. In various aspects, methods and systems in accordance with applicant's teachings can enable a differential mobility spectrometer to resolve biologically relevant acids through the use of CO2 as the drift gas in combination with Acetone.

Abstract translation: 本文提供了用于进行离子迁移光谱法的方法和系统。 根据申请人的教导的各个方面，所述方法和系统可以提供与常规MS技术难以分离的生物相关酸的分离。 在各个方面，根据申请人的教导的方法和系统可以使差示迁移谱仪能够通过使用CO 2作为与丙酮组合的漂移气体来分解生物相关的酸。

公开(公告)号：US20150228464A1

公开(公告)日：2015-08-13

申请号：US14617805

申请日：2015-02-09

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Eva Duchoslav ,  Ronald F. Bonner ,  Lyle Burton

IPC: H01J49/00

CPC classification number: H01J49/004 ,  H01J49/0036

Abstract: A precursor ion spectrum and one or more product ion spectra are received from a tandem mass spectrometer that analyzes a compound. For a selected precursor ion peak in the precursor ion spectrum, mass differences are calculated between other peaks and one or more limitations on the number of elements in the selected precursor ion are generated. For a precursor ion peak, a product ion spectrum is located from the one or more product ion spectra. For a selected product ion peak in the product ion spectrum, mass differences between other peaks are calculated and one or more limitations on the number of elements in the selected product ion are generated. Limitations on the number of elements from the precursor ion and the product ions are combined. One or more elemental compositions are generated from a mass of the selected precursor ion peak and the combined limitations.

Abstract translation: 从分析化合物的串联质谱仪接收前体离子谱和一个或多个产物离子光谱。 对于前体离子谱中所选择的前体离子峰，在其它峰之间计算质量差异，并且对所选择的前体离子中的元素数量产生一个或多个限制。 对于前体离子峰，产物离子光谱位于一个或多个产物离子光谱上。 对于产物离子谱中的选定产物离子峰，计算其它峰之间的质量差，并且产生对所选产物离子中元素数量的一个或多个限制。 来自前体离子和产物离子的元素数目的限制被组合。 从所选择的前体离子峰的质量和组合的限制产生一种或多种元素组合物。

公开(公告)号：US20150129757A1

公开(公告)日：2015-05-14

申请号：US14328550

申请日：2014-07-10

Applicant: DH Technologies Development PTE. LTD.

Inventor： Ronald F. Bonner ,  Stephen A. Tate

IPC: H01J49/00

CPC classification number: H01J49/0081 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/004 ,  H01J49/0045

Abstract: Systems and methods are used to analyze a sample using variable mass selection window widths. A tandem mass spectrometer is instructed to perform at least two fragmentation scans of a sample with different mass selection window widths using a processor. The tandem mass spectrometer includes a mass analyzer that allows variable mass selection window widths. The selection of the different mass selection window widths can be based on one or more properties of sample compounds. The properties may include a sample compound molecular weight distribution that is calculated from a molecular weight distribution of expected compounds or is determined from a list of molecular weights for one or more known compounds. The tandem mass spectrometer can also be instructed to perform an analysis of the sample before instructing the tandem mass spectrometer to perform the at least two fragmentation scans of the sample.

Abstract translation: 系统和方法用于使用可变质量选择窗口宽度来分析样本。 指示串联质谱仪使用处理器对具有不同质量选择窗口宽度的样品进行至少两次碎片扫描。 串联质谱仪包括质量分析器，允许质量选择窗口宽度可变。 不同质量选择窗口宽度的选择可以基于样品化合物的一种或多种性质。 性质可以包括根据预期化合物的分子量分布计算的样品化合物分子量分布，或由一种或多种已知化合物的分子量列表确定。 也可以指示串联质谱仪在指示串联质谱仪进行样品的至少两次碎片扫描之前对样品进行分析。

公开(公告)号：US20150076342A1

公开(公告)日：2015-03-19

申请号：US14395705

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John L. Campbell ,  Thomas Covey ,  Yves LeBlanc ,  Bradley B. Schneider

IPC: H01J49/16 ,  G01N27/62 ,  H01J49/00 ,  H01J49/04

CPC classification number: H01J49/165 ,  G01N27/622 ,  H01J49/0081 ,  H01J49/0431

Abstract: An electrospray ion source method and system is provided for detecting emitter failure comprising a liquid chromatography column suitable for chromatographic separation of a sample. The column can have an inlet for receiving the sample; and an outlet for ejecting the sample. A make-up flow channel is provided for introducing make-up flow of liquid to the sample post-column, wherein the make-up flow normalizes the spray current. An electrospray ionization source is provided having one or more electrospray ionization emitter nozzles for receiving the make-up flow containing sample. A power supply can provide a voltage to the one or more emitter nozzles, and a measurement device can measure and monitor the spray current.

Abstract translation: 提供了一种用于检测发射器故障的电喷雾离子源方法和系统，其包括适于样品色谱分离的液相色谱柱。 该柱可以具有用于接收样品的入口; 以及用于喷射样品的出口。 提供了补充流动通道，用于将液体的补充流引入样品后柱，其中补充流使标准化喷雾电流。 提供了具有一个或多个电喷雾电离发射器喷嘴的电喷雾离子源，用于接收含有补充流的样品。 电源可以向一个或多个发射器喷嘴提供电压，并且测量装置可以测量和监测喷射电流。