公开(公告)号：US20090218028A1

公开(公告)日：2009-09-03

申请号：US12040819

申请日：2008-02-29

Applicant: Tzu-Yu Wang

Inventor： Tzu-Yu Wang

IPC: B29C65/00 ,  B32B5/16 ,  G01J3/44

CPC classification number: G01J3/44 ,  G01N21/658 ,  Y10T156/10 ,  Y10T428/25

Abstract: A surface-enhance Raman scattering (SERS) film is disposed on a portion of an asymmetrical optical coating of a core. The core has diameter in a range from about 10 nanometer (nm) to about 1,000 nm. The asymmetrical optical coating is in contact with a covering the core. The SERS film, the asymmetrical optical coating, and the core make up a particle. The particle is disposed on a mounting substrate.

Abstract translation: 表面增强拉曼散射（SERS）膜设置在芯的不对称光学涂层的一部分上。 芯的直径在约10纳米至约1000纳米的范围内。 不对称光学涂层与覆盖芯部接触。 SERS膜，不对称光学涂层和芯组成一个颗粒。 颗粒设置在安装基板上。

公开(公告)号：US07580126B2

公开(公告)日：2009-08-25

申请号：US11268590

申请日：2005-11-08

Applicant: David Tuschel

Inventor： David Tuschel

IPC: G01J3/44

CPC classification number: G01J3/2889 ,  G01J3/44 ,  G01N21/3563 ,  G01N21/3577 ,  G01N21/65

Abstract: The present disclosure describes methods and apparatus to produce a streaming image of a sample during a time period when an attribute of the sample is changing. The streaming image can be viewed in such a manner so as to be able to follow a visible change in an attribute of the sample. The sample may be undergoing nucleation, aggregation, or chemical interaction. The present disclosure also describes methods and apparatus to determine a change in an attribute of a sample by detecting, analyzing, and comparing spectra of the sample taken at different times during the time period when the attribute of the sample is changing. The sample may be undergoing nucleation, aggregation, or chemical interaction.

Abstract translation: 本公开描述了在样本的属性改变的时间段期间产生样本的流图像的方法和装置。 可以以这样的方式来观看流图像，以便能够跟踪样本的属性的可见变化。 样品可能经历成核，聚集或化学相互作用。 本公开还描述了通过检测，分析和比较在样本的属性正在改变的时间段期间在不同时间拍摄的样本的光谱来确定样本的属性变化的方法和装置。 样品可能经历成核，聚集或化学相互作用。

公开(公告)号：US07573570B2

公开(公告)日：2009-08-11

申请号：US11894402

申请日：2007-08-21

Applicant: Jingyun Zhang

Inventor： Jingyun Zhang

IPC: G01J3/02 ,  G01J3/44 ,  G01N21/64 ,  G01N21/65

CPC classification number: G01J3/44 ,  G01J3/02 ,  G01J3/021 ,  G01J3/0256 ,  G01J3/0272 ,  G01N2201/0221

Abstract: A compact Raman and fluorescence spectroscopy system that uses a microprism or micromirror based optical structure to accomplish the introduction of excitation radiation with compactness and simplified system configuration for portable or mobile spectroscopy applications. A microprism may be glued to a surface location of a focusing lens in the system to directly receive the illumination signal without intervening optical components. Alternatively, the microprism may be simply placed in close physical proximity of the focusing lens without being glued thereto. On the other hand, a micromirror may be used instead of the microprism. The illuminating photons received by the microprism or micromirror may be directly transferred to the sample under investigation via the focusing lens. The compact system may be made portable and may further include an on-board spectrometer with or without a display unit. For chemical detecting applications, a detector (e.g., a CCD array) may also be provided along with the spectrometer.

Abstract translation: 一种紧凑的拉曼和荧光光谱系统，其使用基于微棱镜或微镜的光学结构来实现便携式或移动光谱应用的致密性和简化的系统配置的激发辐射的引入。 微棱镜可以胶合到系统中的聚焦透镜的表面位置，以直接接收照明信号而无需中间的光学部件。 或者，微棱镜可以简单地放置在聚焦透镜的紧密物理附近而不粘合到聚焦透镜上。 另一方面，可以使用微镜而不是微棱镜。 由微棱镜或微反射镜接收的照明光子可以通过聚焦透镜被直接转移到被研究的样品。 紧凑型系统可以是便携式的，并且还可以包括具有或不具有显示单元的车载光谱仪。 对于化学检测应用，也可以与光谱仪一起提供检测器（例如，CCD阵列）。

公开(公告)号：US07561265B2

公开(公告)日：2009-07-14

申请号：US11607491

申请日：2006-12-01

Applicant: Minoru Kobayashi ,  Taisuke Ota ,  Takahiro Ode

Inventor： Minoru Kobayashi ,  Taisuke Ota ,  Takahiro Ode

IPC: G01J3/30

CPC classification number: G01J3/44 ,  G01J3/02 ,  G01J3/021 ,  G01J3/0213 ,  G01J3/0262 ,  G01J2003/1243 ,  G01J2003/1247

Abstract: An optical microscope according to a first embodiment of the present invention includes: a laser light source; a Y-directional scanning unit moving the light beam in a Y direction; an objective lens; a X-directional scanning unit moving the light beam in a X direction; a beam splitter provided in an optical path from the Y-directional scanning unit to the sample, and separating outgoing light out of the light beam incident on the sample, which exits from the sample toward the objective lens from the light beam incident on the sample from the laser light source; a spectroscope having an entrance slit extending along the Y direction and spatially dispersing the outgoing light passed through the entrance slit in accordance with a wavelength of the light; and a detector detecting the outgoing light dispersed by the spectroscope.

Abstract translation: 根据本发明第一实施例的光学显微镜包括：激光源; Y方向扫描单元，沿Y方向移动光束; 物镜; X方向扫描单元，沿X方向移动光束; 设置在从Y方向扫描单元到样本的光路中的分束器，并将入射到样品上的光束从入射到样品上的光束从样品朝向物镜离开的光束分离出来 来自激光光源; 具有沿Y方向延伸的入口狭缝的分光镜，并且根据光的波长对通过入口狭缝的出射光进行空间上的分散; 以及检测器，其检测由分光镜分散的出射光。

公开(公告)号：US07548311B2

公开(公告)日：2009-06-16

申请号：US11475582

申请日：2006-06-27

Applicant: Kevin J. Knopp ,  Peidong Wang ,  Masud Azimi ,  Daryoosh Vakhshoori

Inventor： Kevin J. Knopp ,  Peidong Wang ,  Masud Azimi ,  Daryoosh Vakhshoori

IPC: G01J3/44

CPC classification number: G01J3/02 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/0227 ,  G01J3/0232 ,  G01J3/0272 ,  G01J3/0291 ,  G01J3/44 ,  G01J2003/1213 ,  G01N21/65 ,  G01N2021/656

Abstract: A Raman probe assembly comprises: a light source for generating laser excitation light; a camera for capturing an image; a light analyzer for analyzing a Raman signature; and a light path for (i) delivering the laser excitation light from the light source to the specimen so as to produce the Raman signature for the specimen, (ii) capturing an image of the specimen and directing that image to the camera, and (iii) directing the Raman signature of the specimen to the light analyzer. A method includes providing a Raman probe assembly carried by a remote controlled robot; navigating the remote control robot to a position adjacent to a specimen; opening a shutter/wiper disposed adjacent to a window of the Raman analyzer; using a camera to aim the probe body at the specimen; energizing a light source; and analyzing the return light passed to the light analyzer.

Abstract translation: 拉曼探针组件包括：用于产生激光激发光的光源; 用于捕获图像的相机; 用于分析拉曼签名的光分析器; 以及用于（i）将激光激发光从光源传送到样本以产生用于样本的拉曼特征的光路，（ii）拍摄样本的图像并将该图像引导到相机，以及（ iii）将样品的拉曼标记引导到光分析仪。 一种方法包括提供由遥控机器人携带的拉曼探针组件; 将远程控制机器人导航到与样本相邻的位置; 打开与拉曼分析仪的窗口相邻设置的快门/刮水器; 使用相机将探针主体对准样本; 激励光源; 并分析通过光分析器的返回光。

公开(公告)号：US07542137B2

公开(公告)日：2009-06-02

申请号：US11781602

申请日：2007-07-23

Applicant: Sangeeta Murugkar ,  Hanan Anis ,  Xiaoliang Sunney Xie ,  Conor Evans

Inventor： Sangeeta Murugkar ,  Hanan Anis ,  Xiaoliang Sunney Xie ,  Conor Evans

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01J3/44 ,  G01J3/4338 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/653

Abstract: The invention provides a system and method for automatic real-time monitoring for the presence of a pathogen in water using coherent anti-stokes Raman scattering (CARS) microscopy. Water sample trapped in a trapping medium is provided to a CARS imager. CARS images are provided to a processor for automatic analyzing for the presence of image artifacts having pre-determined features characteristic to the pathogen. If a match is found, a CARS spectrum is taken and compared to a stored library of reference pathogen-specific spectra for pathogen identification. The system enables automatic pathogen detection in flowing water in real time.

Abstract translation: 本发明提供一种用于使用相干抗斯托克斯拉曼散射（CARS）显微镜自动实时监测水中病原体存在的系统和方法。 捕获介质中的水样被提供给CARS成像仪。 将CARS图像提供给处理器，用于自动分析具有对病原体特征的预定特征的图像伪影的存在。 如果发现匹配，则采集CARS光谱，并将其与用于病原体鉴定的参考病原体特异性光谱的存储库进行比较。 该系统实时实现流水中的自动病原体检测。

公开(公告)号：US07525653B1

公开(公告)日：2009-04-28

申请号：US11245486

申请日：2005-10-05

Applicant: William F. Hug ,  Ray D. Reid

Inventor： William F. Hug ,  Ray D. Reid

IPC: G01J3/44

CPC classification number: G01N21/65 ,  G01J3/10 ,  G01J3/36 ,  G01J3/44 ,  G01N21/645 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2201/0612 ,  G01N2201/067

Abstract: Spectroscopic chemical analysis methods and apparatus are disclosed which employ deep ultraviolet (e.g. in the 200 nm to 300 nm spectral range) electron beam pumped wide bandgap semiconductor lasers, incoherent wide bandgap semiconductor light emitting devices, and hollow cathode metal ion lasers to perform non-contact, non-invasive detection of unknown chemical analytes. These deep ultraviolet sources enable dramatic size, weight and power consumption reductions of chemical analysis instruments. Chemical analysis instruments employed in some embodiments include capillary and gel plane electrophoresis, capillary electrochromatography, high performance liquid chromatography, flow cytometry, flow cells for liquids and aerosols, and surface detection instruments. In some embodiments, Raman spectroscopic detection methods and apparatus use ultra-narrow-band angle tuning filters, acousto-optic tuning filters, and temperature tuned filters to enable ultra-miniature analyzers for chemical identification. In some embodiments Raman analysis is conducted simultaneously with native fluorescence spectroscopy to provide high levels of sensitivity and specificity in the same instrument.

Abstract translation: 公开了使用深紫外线（例如在200nm至300nm光谱范围内）的电子束泵浦宽带隙半导体激光器，非相干宽带隙半导体发光器件和空心阴极金属离子激光器的光谱化学分析方法和装置， 接触，未知化学分析物的非侵入性检测。 这些深紫外线源可以显着降低化学分析仪器的体积，重量和功耗。 在一些实施方案中使用的化学分析仪器包括毛细管和凝胶平面电泳，毛细管电色谱，高效液相色谱，流式细胞术，液体和气溶胶流动池以及表面检测仪器。 在一些实施例中，拉曼光谱检测方法和装置使用超窄带角度调谐滤波器，声光调谐滤波器和温度调节滤波器，以使超微型分析仪能够进行化学鉴定。 在一些实施方案中，拉曼分析与天然荧光光谱同时进行，以在同一仪器中提供高水平的灵敏度和特异性。

公开(公告)号：US20090099458A9

公开(公告)日：2009-04-16

申请号：US11570669

申请日：2005-06-20

Applicant: Mark Towler ,  Declan Lyons

Inventor： Mark Towler ,  Declan Lyons

IPC: A61B18/20

CPC classification number: A61B5/0075 ,  A61B5/0059 ,  A61B5/441 ,  A61B5/4504 ,  A61B5/4509 ,  G01J3/44

Abstract: Methods of diagnosing bone disease such as osteoporosis are provided. The methods comprise detecting changes in the physical or chemical structure of a keratinized tissue as correlates of disease. The methods include detecting changes in the hardness, modulus, or level of sulfur bonding, particularly the level of disulfide bonding, in a keratinized tissue sample such as nail, hair, or skin. Changes in these variables serve as diagnostic markers of bone diseases that are associated with changes in bone elasticity and bone density.

Abstract translation: 提供诊断骨质疏松症等骨疾病的方法。 所述方法包括检测角质化组织的物理或化学结构的变化作为疾病的相关性。 所述方法包括检测角化组织样品如指甲，毛发或皮肤中的硫键的硬度，模量或水平的变化，特别是二硫键的水平。 这些变量的变化用作与骨骼弹性和骨密度变化相关的骨骼疾病的诊断标记。

公开(公告)号：US07499153B2

公开(公告)日：2009-03-03

申请号：US10537074

申请日：2003-12-02

Applicant: Gerwin Jan Puppels ,  Rolf Wolthuis ,  Senada Koljenovic

Inventor： Gerwin Jan Puppels ,  Rolf Wolthuis ,  Senada Koljenovic

IPC: G01J3/44 ,  G01N21/64 ,  G01N21/65

CPC classification number: G01N21/65 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0086 ,  A61B5/441 ,  A61B5/4848 ,  G01J3/44 ,  G01N2021/656

Abstract: The invention is related to the instrument for measuring a Raman signal of tissue, the instrument comprising a laser, a signal detection unit for measuring the Raman signal, and a fiber optic probe, wherein the fiber optic probe comprises one or more optical fibers for directing laser light onto the tissue and for collecting light that is scattered by the tissue and guiding the collected light away from the tissue towards the signal detection unit, wherein the fiber or fibers for collecting light have substantially no Raman signal in one or more parts of the 2500-3700 cm−1 spectral region, and wherein the detection unit records the Raman signal scattered by the tissue in said spectral region. The invention enables ex vivo, in vitro and in vivo analysis and diagnosis of atherosclerotic plaque and detection of tumor tissue with great advantages over current state-of-the-art technology.

Abstract translation: 本发明涉及用于测量组织的拉曼信号的仪器，仪器包括激光器，用于测量拉曼信号的信号检测单元和光纤探针，其中光纤探针包括一根或多根光纤，用于引导 将激光照射到组织上并用于收集由组织散射的光并将收集的光引导离开组织朝向信号检测单元，其中用于收集光的光纤或纤维在一个或多个部分中基本上不具有拉曼信号 2500-3700cm -1光谱区域，并且其中所述检测单元记录由所述光谱区域中的组织散射的拉曼信号。 本发明使得动脉粥样硬化斑块的离体，体外和体内分析和诊断和肿瘤组织的检测具有优于现有技术的优点。

公开(公告)号：US20080319324A1

公开(公告)日：2008-12-25

申请号：US12206500

申请日：2008-09-08

Applicant: John Maier ,  Shona Stewart ,  Jeffrey Cohen

Inventor： John Maier ,  Shona Stewart ,  Jeffrey Cohen

IPC: A61B6/00

CPC classification number: G01J3/02 ,  A61B5/0059 ,  A61B5/417 ,  G01J3/0291 ,  G01J3/0294 ,  G01J3/28 ,  G01J3/2823 ,  G01J3/36 ,  G01J3/44 ,  G01J2003/2826 ,  G01N21/65 ,  G01N33/57415 ,  G01N2021/656 ,  G01N2201/1293 ,  G01N2201/1296 ,  Y02A90/26

Abstract: A system and method to provide a diagnosis of the breast disease state of a test breast sample. A database containing a plurality of reference Raman data sets is provided where each reference Raman data set has an associated known breast sample and an associated known breast disease state. A test breast sample is irradiated with substantially monochromatic light to generate scattered photons resulting in a test Raman data set. The test Raman data set is compared to the plurality of reference Raman data sets using a chemometric technique. Based on the comparison, a diagnosis of a breast disease state of the test breast sample is provided. The breast disease state includes invasive ductal carcinoma or invasive lobular carcinoma disease state.

Abstract translation: 一种用于提供测试乳房样品的乳腺疾病状态诊断的系统和方法。 提供了包含多个参考拉曼数据集的数据库，其中每个参考拉曼数据集具有相关联的已知乳房样本和相关联的已知乳腺疾病状态。 用基本上单色的光照射测试乳房样品以产生散射光子，得到测试拉曼数据集。 使用化学计量技术将测试拉曼数据集与多个参考拉曼数据集进行比较。 基于比较，提供了测试乳房样品的乳腺疾病状态的诊断。 乳腺疾病状态包括浸润性导管癌或侵袭性小叶癌疾病状态。